Cloning and characterization of the DNA region responsible for Megacin A-216 production in Bacillus megaterium 216.

abstract：:Haloarchaea in their natural environment are exposed to hypersalinity, intense solar radiation, and desiccation, all of which generate high levels of oxidative stress. Previous work has shown that haloarchaea are an order of magnitude more resistant to oxidative stress than most mesophilic organisms. Despite this resi...

journal_title：Journal of bacteriology

authors： Gelsinger DR,DiRuggiero J

更新日期：2018-04-09 00:00:00

abstract：:The two-component regulatory system, composed of virA and virG, is indispensable for transcription of virulence genes within Agrobacterium tumefaciens. However, virA and virG are insufficient to activate transcription from virulence gene promoters within Escherichia coli cells, indicating a requirement for additional ...

journal_title：Journal of bacteriology

authors： Lohrke SM,Nechaev S,Yang H,Severinov K,Jin SJ

更新日期：1999-08-01 00:00:00

abstract：:In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. Th...

journal_title：Journal of bacteriology

authors： Oliver EJ,Mortlock RP

更新日期：1971-10-01 00:00:00

abstract：:A new pathway for aerobic benzoate oxidation has been postulated for Azoarcus evansii and for a Bacillus stearothermophilus-like strain. Benzoate is first transformed into benzoyl coenzyme A (benzoyl-CoA), which subsequently is oxidized to 3-hydroxyadipyl-CoA and then to 3-ketoadipyl-CoA; all intermediates are CoA thi...

journal_title：Journal of bacteriology

authors： Gescher J,Zaar A,Mohamed M,Schägger H,Fuchs G

更新日期：2002-11-01 00:00:00

abstract：:To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. cit...

journal_title：Journal of bacteriology

authors： Duret S,Danet JL,Garnier M,Renaudin J

更新日期：1999-12-01 00:00:00

abstract：:The gene encoding an extracellular isomalto-dextranase, designated imd, was isolated from the chromosomal DNA of Arthrobacter globiformis T6 and cloned and expressed in Escherichia coli. A single open reading frame consisting of 1,926 base pairs that encoded a polypeptide composed of a signal peptide of 39 amino acids...

journal_title：Journal of bacteriology

authors： Iwai A,Ito H,Mizuno T,Mori H,Matsui H,Honma M,Okada G,Chiba S

更新日期：1994-12-01 00:00:00

abstract：:DnaA is an AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load the replicative helicase. Dna...

journal_title：Journal of bacteriology

authors： Bonilla CY,Grossman AD

更新日期：2012-09-01 00:00:00

abstract：:On the basis of the constitutive phenotypes of two catM mutants of Acinetobacter calcoaceticus, the CatM protein was proposed to repress expression of two different loci involved in catechol degradation, catA and catBCIJFD (E. Neidle, C. Hartnett, and L. N. Ornston, J. Bacteriol. 171:5410-5421, 1989). In spite of its ...

journal_title：Journal of bacteriology

authors： Romero-Arroyo CE,Schell MA,Gaines GL 3rd,Neidle EL

更新日期：1995-10-01 00:00:00

abstract：:Flavobacterium johnsoniae cells move rapidly over surfaces by gliding motility. Gliding results from the movement of adhesins such as SprB and RemA along the cell surface. These adhesins are delivered to the cell surface by a Bacteroidetes-specific secretion system referred to as the type IX secretion system (T9SS). G...

journal_title：Journal of bacteriology

authors： Shrivastava A,Johnston JJ,van Baaren JM,McBride MJ

更新日期：2013-07-01 00:00:00

abstract：:The Escherichia coli K-12 hisT gene has been cloned, and its organization and expression have been analyzed on multicopy plasmids. The hisT gene, which encodes tRNA pseudouridine synthase I (PSUI), was isolated on a Clarke-Carbon plasmid known to contain the purF gene. The presence of the hisT gene on this plasmid was...

journal_title：Journal of bacteriology

authors： Marvel CC,Arps PJ,Rubin BC,Kammen HO,Penhoet EE,Winkler ME

更新日期：1985-01-01 00:00:00

abstract：:The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escherichia coli lacZ was forced to form tandem repeats by the selection of clones tha...

journal_title：Journal of bacteriology

authors： Piggot PJ,Curtis CA

更新日期：1987-03-01 00:00:00

abstract：:Bacillus subtilis was exposed to ultraviolet light (UV) or X rays, and gene frequency analysis was used to study the location of initiation sites of postirradiation deoxyribonucleic acid (DNA) synthesis. It was found that DNA synthesis resumes primarily from the origin after UV exposure. With X irradiation, the origin...

journal_title：Journal of bacteriology

authors： Billen D,Hellerman G,Carreira L

更新日期：1972-01-01 00:00:00

abstract：:An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

journal_title：Journal of bacteriology

authors： Woldringh CL,van Iterson W

更新日期：1972-09-01 00:00:00

abstract：:The Marburg strain of Butyribacterium methylotrophicum did not grow on CO alone but did consume CO during growth on a variety of substrates in the presence of a 100% CO gas phase. We selected a strain (the CO strain) that grew vigorously on CO alone. The ability of the CO strain to grow on CO was stable through multip...

journal_title：Journal of bacteriology

authors： Lynd L,Kerby R,Zeikus JG

更新日期：1982-01-01 00:00:00

abstract：:The extracytoplasmic factor (ECF) sigma factor sigma(E) is one of the most studied sigma factors of Mycobacterium tuberculosis. It has been shown to be involved in virulence as well as in survival under conditions of high temperature, alkaline pH, and exposure to detergents and oxidative stress. Unlike many ECF sigma ...

journal_title：Journal of bacteriology

authors： Donà V,Rodrigue S,Dainese E,Palù G,Gaudreau L,Manganelli R,Provvedi R

更新日期：2008-09-01 00:00:00

abstract：:The endophytic diazotroph Azoarcus sp. strain BH72 is capable of infecting rice roots and of expressing the nitrogenase (nif) genes there. In order to study the genetic background for nitrogen fixation in strain BH72, the structural genes of nitrogenase (nifHDK) were cloned and sequenced. The sequence analysis reveale...

journal_title：Journal of bacteriology

authors： Egener T,Martin DE,Sarkar A,Reinhold-Hurek B

更新日期：2001-06-01 00:00:00

abstract：:Fimbriae recognizing sialyl galactosides (S fimbriae) were purified from an Escherichia coli strain. The S fimbriae were morphologically identical to type 1 and P fimbriae of E. coli and showed a hemagglutination that was abolished when erythrocytes were treated with neuraminidase. Hemagglutination by the purified fim...

journal_title：Journal of bacteriology

authors： Korhonen TK,Väisänen-Rhen V,Rhen M,Pere A,Parkkinen J,Finne J

更新日期：1984-08-01 00:00:00

abstract：:Entry into the host bacterial cell is one of the least understood steps in the life cycle of bacteriophages. The different envelopes of Gram-negative and Gram-positive bacteria, with a fluid outer membrane and exposing a thick peptidoglycan wall to the environment respectively, impose distinct challenges for bacteriop...

journal_title：Journal of bacteriology

authors： Jakutytė L,Baptista C,São-José C,Daugelavičius R,Carballido-López R,Tavares P

更新日期：2011-09-01 00:00:00

abstract：:H10407 is a strain of enterotoxigenic Escherichia coli (ETEC) that utilizes CFA/I pili to adhere to surfaces of the small intestine, where it elaborates toxins that cause profuse watery diarrhea in humans. Expression of the CFA/I pilus is positively regulated at the level of transcription by CfaD, a member of the AraC...

journal_title：Journal of bacteriology

authors： Pilonieta MC,Bodero MD,Munson GP

更新日期：2007-07-01 00:00:00

abstract：:An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, J. Olivares, and J. Sanjuán, J. Bacteriol. 180:4583-4590, 1998) was modified to improve its reliability and prevent selection of undesired false mob ...

journal_title：Journal of bacteriology

authors： Pérez-Mendoza D,Domínguez-Ferreras A,Muñoz S,Soto MJ,Olivares J,Brom S,Girard L,Herrera-Cervera JA,Sanjuán J

更新日期：2004-09-01 00:00:00

abstract：:Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely lim...

journal_title：Journal of bacteriology

authors： McCusker JH,Haber JE

更新日期：1977-10-01 00:00:00

abstract：:The twisting and writhing during growth of single-cell filaments of Bacillus subtilis which lead to macrofiber formation was studied in both left- and right-handed forms of strains FJ7 and RHX. Filament bending, touching, and loop formation (folding), followed by winding up into a double-strand fiber, were documented....

journal_title：Journal of bacteriology

authors： Mendelson NH,Thwaites JJ,Kessler JO,Li C

更新日期：1995-12-01 00:00:00

abstract：:The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with p...

journal_title：Journal of bacteriology

authors： Yokota K,Kanamoto R,Kito M

更新日期：1980-03-01 00:00:00

abstract：:Two clindamycin-erythromycin resistance transfer factors of Bacteroides fragilis, pBF4 and pBFTM10, were analyzed for regions of DNA homology. Although the plasmids were derived from different clinical isolates of B. fragilis and have different sizes, they showed homology in the clindamycin-erythromycin resistance reg...

journal_title：Journal of bacteriology

authors： Shimell MJ,Smith CJ,Tally FP,Macrina FL,Malamy MH

更新日期：1982-11-01 00:00:00

abstract：:The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides ...

journal_title：Journal of bacteriology

authors： Reder A,Höper D,Gerth U,Hecker M

更新日期：2012-07-01 00:00:00

abstract：:A new bacteriocin, termed lactococcin A (LCN-A), from Lactococcus lactis subsp. cremoris LMG 2130 was purified and sequenced. The polypeptide contained no unusual amino acids and showed no significant sequence similarity to other known proteins. Only lactococci were killed by the bacteriocin. Of more than 120 L. lacti...

journal_title：Journal of bacteriology

authors： Holo H,Nilssen O,Nes IF

更新日期：1991-06-01 00:00:00

abstract：:The replication of the R factor Rts1 in Proteus mirabilis was examined by using the technique of CsCl density-gradient centrifugation. The proportion of Rts1 deoxyribonucleic acid (DNA) relative to the host chromosomal DNA (% R-DNA) was 7% in both exponential and stationary growth phases in Penassay Broth and suppleme...

journal_title：Journal of bacteriology

authors： Terawaki Y,Rownd R

更新日期：1972-02-01 00:00:00

abstract：:Nosocomial infections caused by antibiotic-resistant Klebsiella pneumoniae are emerging as a major health problem worldwide, while community-acquired K. pneumoniae infections present with a range of diverse clinical pictures in different geographic areas. In particular, an invasive form of K. pneumoniae that causes li...

journal_title：Journal of bacteriology

authors： Wu KM,Li LH,Yan JJ,Tsao N,Liao TL,Tsai HC,Fung CP,Chen HJ,Liu YM,Wang JT,Fang CT,Chang SC,Shu HY,Liu TT,Chen YT,Shiau YR,Lauderdale TL,Su IJ,Kirby R,Tsai SF

更新日期：2009-07-01 00:00:00

abstract：:Genetic exchange mechanisms, to our knowledge, have not been reported for Legionella pneumophila, and consequently, studies on the genetic organization of L. pneumophila have not appeared in the literature. Here, we describe gene transfer mediated by broad host range conjugative plasmids in Legionella spp. Escherichia...

journal_title：Journal of bacteriology

authors： Dreyfus LA,Iglewski BH

更新日期：1985-01-01 00:00:00

abstract：:The Mycobacterium tuberculosis gene Rv2747 encodes a novel 19-kDa ArgA that catalyzes the initial step in L-arginine biosynthesis, namely the conversion of L-glutamate to alpha-N-acetyl-L-glutamate. Initial velocity studies reveal that Rv2747 proceeds through a sequential kinetic mechanism, with K(m) values of 280 mM ...

journal_title：Journal of bacteriology

authors： Errey JC,Blanchard JS

更新日期：2005-05-01 00:00:00