Abstract:
:The two-component regulatory system, composed of virA and virG, is indispensable for transcription of virulence genes within Agrobacterium tumefaciens. However, virA and virG are insufficient to activate transcription from virulence gene promoters within Escherichia coli cells, indicating a requirement for additional A. tumefaciens genes. In a search for these additional genes, we have identified the rpoA gene, encoding the alpha subunit of RNA polymerase (RNAP), which confers significant expression of a virB promoter (virBp)::lacZ fusion in E. coli in the presence of an active transcriptional regulator virG gene. We conducted in vitro transcription assays using either reconstituted E. coli RNAP or hybrid RNAP in which the alpha subunit was derived from A. tumefaciens. The two forms of RNAP were equally efficient in transcription from a sigma(70)-dependent E. coli galP1 promoter; however, only the hybrid RNAP was able to transcribe virBp in a virG-dependent manner. In addition, we provide evidence that the alpha subunit from A. tumefaciens, but not from E. coli, is able to interact with the VirG protein. These data suggest that transcription of virulence genes requires specific interaction between VirG and the alpha subunit of A. tumefaciens and that the alpha subunit from E. coli is unable to effectively interact with the VirG protein. This work provides the basis for future studies designed to examine vir gene expression as well as the T-DNA transfer process in E. coli.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lohrke SM,Nechaev S,Yang H,Severinov K,Jin SJdoi
10.1128/JB.181.15.4533-4539.1999keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
4533-9issue
15eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01869-06
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abstract::Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1621-1623.1975
更新日期:1975-12-01 00:00:00
abstract::We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no m...
journal_title:Journal of bacteriology
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doi:10.1128/jb.170.8.3513-3522.1988
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abstract::Beppu, Michiko (University of Tokyo, Tokyo, Japan), and Kei Arima. Decreased permeability as the mechanism of arsenite resistance in Pseudomonas pseudomallei. J. Bacteriol. 88:151-157. 1964.-The mechanism of arsenite resistance of Pseudomonas pseudomallei strain 54, isolated from soil, was studied by use of radioactiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.151-157.1964
更新日期:1964-07-01 00:00:00
abstract:UNLABELLED:Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02564-14
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.2.843-848.1975
更新日期:1975-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5539-5544.1988
更新日期:1988-12-01 00:00:00
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更新日期:2005-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.141-148.1988
更新日期:1988-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.107.3.806-814.1971
更新日期:1971-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.9.2619-2626.1994
更新日期:1994-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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更新日期:2014-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1975-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.7.2124-2131.1999
更新日期:1999-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1969-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.8.2425-2430.2001
更新日期:2001-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.918-923.1982
更新日期:1982-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.1.1-6.1973
更新日期:1973-04-01 00:00:00