Nuclear deoxyribonucleic acid-dependent ribonucleic acid polymerases from Saccharomyces cerevisiae.

Abstract:

:Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA polymerase I has a higher affinity for polydeoxy-adenylic-thymidylic acid and RNA polymerase II for denatured DNA. RNA polymerase I is active only with manganese. alpha-Amanitin inhibits only the activity of RNA polymerase II.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sebastian J,Bhargava MM,Halvorson HO

doi

10.1128/JB.114.1.1-6.1973

subject

Has Abstract

pub_date

1973-04-01 00:00:00

pages

1-6

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

114

pub_type

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