Abstract:
:A search by subtractive hybridization for sequences present in only certain strains of Helicobacter pylori led to the discovery of a 2-kb transposable element to be called IS607, which further PCR and hybridization tests indicated was present in about one-fifth of H. pylori strains worldwide. IS607 contained two open reading frames (ORFs) of possibly different phylogenetic origin. One ORF (orfB) exhibited protein-level homology to one of two putative transposase genes found in several other chimeric elements including IS605 (also of H. pylori) and IS1535 (of Mycobacterium tuberculosis). The second IS607 gene (orfA) was unrelated to the second gene of IS605 and might possibly be chimeric itself: it exhibited protein-level homology to merR bacterial regulatory genes in the first approximately 50 codons and homology to the second gene of IS1535 (annotated as "resolvase," apparently due to a weak short recombinase motif) in the remaining three-fourths of its length. IS607 was found to transpose in Escherichia coli, and analyses of sequences of IS607-target DNA junctions in H. pylori and E. coli indicated that it inserted either next to or between adjacent GG nucleotides, and generated either a 2-bp or a 0-bp target sequence duplication, respectively. Mutational tests showed that its transposition in E. coli required orfA but not orfB, suggesting that OrfA protein may represent a new, previously unrecognized, family of bacterial transposases.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kersulyte D,Mukhopadhyay AK,Shirai M,Nakazawa T,Berg DEdoi
10.1128/jb.182.19.5300-5308.2000keywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
5300-8issue
19eissn
0021-9193issn
1098-5530journal_volume
182pub_type
杂志文章abstract::The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.973-976.1982
更新日期:1982-05-01 00:00:00
abstract::The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.9.2635-2638.2000
更新日期:2000-05-01 00:00:00
abstract::Fitz-James, Philip (University of Western Ontario, London, Canada). Electron microscopy of Bacillus megaterium undergoing isolation of its nucelar bodies. J. Bacteriol. 87:1202-1210. 1964.-The various steps of treatment leading to the isolation of nuclear bodies were followed by thin-section electron microscopy. Nucle...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.1202-1210.1964
更新日期:1964-05-01 00:00:00
abstract::Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.15.4700-4706.1991
更新日期:1991-08-01 00:00:00
abstract::In this communication we present evidence that indicates that 1,2-propanediol (1,2-PDL) is a positive effector of the transcription of the cobalamin (vitamin B12) biosynthetic (cob) operon and of the 1,2-PDL utilization (pdu) genes. The stimulatory effects of 1,2-PDL were demonstrated using Mu d-lac transcriptional fu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.7.2267-2272.1992
更新日期:1992-04-01 00:00:00
abstract::Escherichia coli cells are shown to be attracted to the l-amino acids alanine, asparagine, aspartate, cysteine, glutamate, glycine, methionine, serine, and threonine, but not to arginine, cystine, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, tryptophan, tyrosine, or valine. Bacteria grown in a pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.315-326.1972
更新日期:1972-10-01 00:00:00
abstract::In wild-type Escherichia coli the activity of the maltose transport system is dependent on a periplasmic maltose-binding protein. It has been possible, however, to isolate mutants in which transport activity is mediated by the membrane components of the system and is no longer dependent on the periplasmic binding prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.918-922.1986
更新日期:1986-03-01 00:00:00
abstract::A marked difference was found to exist between the nicotinamide adenine dinucleotide (NAD) glycohydrolase activity of human strains of Mycobacterium tuberculosis as compared with bovine strains. Human strains had from 6- to 20-fold higher NAD glycohydrolase activity than bovine strains. This finding explains the accum...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.2.600-603.1972
更新日期:1972-05-01 00:00:00
abstract::Triggering of germination in Bacillus megaterium QM B1551 spores with D-glucose was studied. First, the interaction of glucose with spores for less than 1 min resulted in triggering almost 90% of the spores after the glucose was removed by dilution. Therefore only a brief time is needed for glucose to trigger germinat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.442-445.1979
更新日期:1979-05-01 00:00:00
abstract::AdnA is a transcription factor in Pseudomonas fluorescens that affects flagellar synthesis, biofilm formation, and sand adhesion. To identify the AdnA regulon, we used a promoterless Tn5-lacZ element to study the phenotypes of insertion mutants in the presence and absence of AdnA. Of 12,000 insertions, we identified s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.2.453-460.2003
更新日期:2003-01-01 00:00:00
abstract::The emerging coverage of diverse habitats by metagenomic shotgun data opens new avenues of discovering functional novelty using computational tools. Here, we apply three different concepts for predicting novel functions within light-mediated microbial pathways in five diverse environments. Using phylogenetic approache...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.01084-08
更新日期:2009-01-01 00:00:00
abstract::Through hybridization of available probes, we have added nine genes to the macrorestriction map of the Haloferax mediterranei chromosome and five genes to the contig map of Haloferax volcanii. Additionally, we hybridized 17 of the mapped cosmid clones from H. volcanii to the H. mediterranei genome. The resulting 35-po...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.5.1405-1408.1995
更新日期:1995-03-01 00:00:00
abstract::The nucleic acid binding cold shock proteins (CSPs) and the cold-induced DEAD box RNA helicases have been proposed separately to act as RNA chaperones, but no experimental evidence has been reported on a direct cooperation. To investigate the possible interaction of the putative RNA helicases CshA and CshB and the CSP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.1.240-248.2006
更新日期:2006-01-01 00:00:00
abstract::In a previous study, we showed that in bacteroids, transcription of the inducible nod genes does not occur and expression of nodD is decreased by 65% (H. R. M. Schlaman, B. Horvath, E. Vijgenboom, R.J.H. Okker, and B. J. J. Lugtenberg, J. Bacteriol. 173:4277-4287, 1991). In the present study, we show, using gel retard...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6109-6116.1992
更新日期:1992-10-01 00:00:00
abstract::Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05454-11
更新日期:2011-10-01 00:00:00
abstract::The overlapping fdhA and fdhB genes of Methanobacterium formicicum, which encode the alpha and beta subunits, respectively, of formate dehydrogenase, were cotranscribed as part of an approximately 4.5-kb transcript. An additional gene (fdhC) upstream of fdhA was cotranscribed with fdhA and fdhB. The deduced amino acid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.4997-5004.1992
更新日期:1992-08-01 00:00:00
abstract::The filamentous bacterium Streptomyces coelicolor forms an aerial mycelium as a prerequisite to sporulation, which occurs in the aerial hyphae. Uncontrolled activity of the extracytoplasmic function sigma factor sigmaU blocks the process of aerial mycelium formation in this organism. Using a green fluorescent protein ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01759-07
更新日期:2008-02-01 00:00:00
abstract::Saccharomyces cerevisiae was grown in a continuous culture at a single dilution rate with input ammonia concentrations whose effects ranged from nitrogen limitation to nitrogen excess and glucose limitation. The rate of ammonia assimilation (in millimoles per gram of cells per hour) was approximately constant. Increas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.22.6672-6675.1995
更新日期:1995-11-01 00:00:00
abstract::A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.887-891.1976
更新日期:1976-03-01 00:00:00
abstract::We have investigated the evolutionary relationships between two facultatively anaerobic Magnetospirillum strains (AMB-1 and MGT-1) and fastidious, obligately microaerophilic species, such as Magnetospirillum magnetotacticum, using a molecular phylogenetic approach. Genomic DNA from strains MGT-1 and AMB-1 was used as ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.20.6689-6694.1993
更新日期:1993-10-01 00:00:00
abstract::When Mucor hiemalis NRRL 3103 was grown in soybean medium, only a small fraction of the proteinase produced by the organism appeared in the culture filtrate, whereas the bulk of the enzyme was bound to the mycelial surface. Optimal pH of the proteinase ranged from 3.0 to 3.5. Inclusion of sodium chloride or other ioni...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.6.1794-1799.1967
更新日期:1967-06-01 00:00:00
abstract::We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1 and PUT2, also required a functional GLN3 protein. In addition, GLN3 protein was required for expression of the DAL1, DA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.1.64-73.1993
更新日期:1993-01-01 00:00:00
abstract::Myxococcus xanthus RB5, a rough-colony-forming, nondispersed growing mutant of strain FBt, forms macroscopic, multicellular masses of radially oriented cells in shake cultures. The cells appear to be held together by slime fibrils. Physical and enzymatic methods to disrupt the spheres were unsuccessful as were attempt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.1.302-306.1975
更新日期:1975-04-01 00:00:00
abstract::A ubiquinone-deficient mutant, carrying mutations in two genes affecting ubiquinone biosynthesis, has been used, in comparison with a normal strain, to determine the sequence of some of the components of the electron transport chain of Escherichia coli. The amounts of cytochromes reduced during aerobic steady-state co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.477-484.1978
更新日期:1978-02-01 00:00:00
abstract::Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05851-11
更新日期:2011-10-01 00:00:00
abstract::Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.279-287.1984
更新日期:1984-10-01 00:00:00
abstract::In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD+- or NADP+-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. The predicted zwf gene product is 490 residues, which could form a tetramer with a molecular m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.7.1741-1749.1998
更新日期:1998-04-01 00:00:00
abstract::In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3429-3438.1992
更新日期:1992-06-01 00:00:00
abstract::The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 A reso...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1712-1718.2003
更新日期:2003-03-01 00:00:00
abstract::Phage KB1 has gained use as a generalized transducing phage for Salmonella typhimurium, including strains resistant to phage P22. Integration-defective mutants of KB1 have now been isolated; one of these, int-1, is recommended for transduction when nonlysogenic recombinants are desired. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.2.907-908.1974
更新日期:1974-02-01 00:00:00