Metabolism of nicotinamide adenine dinucleotide in human and bovine strainsof Mycobacterium tuberculosis.

abstract：:Adenylate cyclases (ACs) catalyze the formation of 3',5'-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC, was isolated from Rhizobium etli by phenotypic complementation of an Escherichia coli cya mutant. The functionality of the cyaC gene was corroborated by its ability to restore cAMP accumulation in an E....

journal_title：Journal of bacteriology

authors： Téllez-Sosa J,Soberón N,Vega-Segura A,Torres-Márquez ME,Cevallos MA

更新日期：2002-07-01 00:00:00

abstract：:Recent studies of anaerobic toluene catabolism have demonstrated a novel reaction for anaerobic hydrocarbon activation: the addition of the methyl carbon of toluene to fumarate to form benzylsuccinate. In vitro studies of the anaerobic benzylsuccinate synthase reaction indicate that the H atom abstracted from the tolu...

journal_title：Journal of bacteriology

authors： Beller HR,Spormann AM

更新日期：1998-10-01 00:00:00

abstract：:A modified 3-hydroxypropionate cycle has been proposed as the autotrophic CO2 fixation pathway for the thermoacidophilic crenarchaeon Metallosphaera sedula. The cycle requires the reductive conversion of 3-hydroxypropionate to propionyl-coenzyme A (propionyl-CoA). The specific activity of the 3-hydroxypropionate-, CoA...

journal_title：Journal of bacteriology

authors： Alber BE,Kung JW,Fuchs G

更新日期：2008-02-01 00:00:00

abstract：:Based on its genome sequence, the pathway of beta-oxidative fatty acid degradation in Salmonella enterica serovar Typhimurium LT2 has been thought to be identical to the well-characterized Escherichia coli K-12 system. We report that wild-type strains of S. enterica grow on decanoic acid, whereas wild-type E. coli str...

journal_title：Journal of bacteriology

authors： Iram SH,Cronan JE

更新日期：2006-01-01 00:00:00

abstract：:The extent of change in cytoplasmic proteins which accompanies yeast-to-mycelium morphogenesis of Candida albicans was analyzed by two-dimensional gel electrophoresis. Pure cultures of yeasts and true hyphae (i.e., without concomitant production of pseudohyphae) were grown in a synthetic low-sulfate medium. The two st...

journal_title：Journal of bacteriology

authors： Manning M,Mitchell TG

更新日期：1980-10-01 00:00:00

abstract：:The pelA and pelC genes from Erwinia chrysanthemi EC16 were sequenced and overexpressed in Escherichia coli cells. These genes and two others from the same strain that were characterized previously encode catalytically related pectate lyase proteins that are involved with the maceration and soft-rotting of plant tissu...

journal_title：Journal of bacteriology

authors： Tamaki SJ,Gold S,Robeson M,Manulis S,Keen NT

更新日期：1988-08-01 00:00:00

abstract：:Rotation of the bacterial flagellar motor is powered by a transmembrane gradient of protons or, in some species, sodium ions. The molecular mechanism of coupling between ion flow and motor rotation is not understood. The proteins most closely involved in motor rotation are MotA, MotB, and FliG. MotA and MotB are trans...

journal_title：Journal of bacteriology

authors： Zhou J,Sharp LL,Tang HL,Lloyd SA,Billings S,Braun TF,Blair DF

更新日期：1998-05-01 00:00:00

abstract：:Campylobacter jejuni is a leading food-borne pathogen causing gastroenteritis in humans. Although OxyR is a widespread oxidative stress regulator in many Gram-negative bacteria, C. jejuni lacks OxyR and instead possesses the metalloregulator PerR. Despite the important role played by PerR in oxidative stress defense, ...

journal_title：Journal of bacteriology

authors： Kim M,Hwang S,Ryu S,Jeon B

更新日期：2011-11-01 00:00:00

abstract：:The nth and nei genes of Escherichia coli affect the production of endonuclease III and endonuclease VIII, respectively, glycosylases/apurinic lyases that attack DNA damaged by oxidizing agents. Here, we provide evidence that oxidative lethal lesions are repaired by both endonuclease III and endonuclease VIII and that...

journal_title：Journal of bacteriology

authors： Saito Y,Uraki F,Nakajima S,Asaeda A,Ono K,Kubo K,Yamamoto K

更新日期：1997-06-01 00:00:00

abstract：:Vibrio cholerae is a highly motile bacterium which possesses a single polar flagellum as a locomotion organelle. Motility is thought to be an important factor for the virulence of V. cholerae. The genome sequencing project of this organism is in progress, and the genes that are highly homologous to the essential genes...

journal_title：Journal of bacteriology

authors： Kojima S,Yamamoto K,Kawagishi I,Homma M

更新日期：1999-03-01 00:00:00

abstract：:Streptococcus pyogenes (group A streptococcus [GAS]), a catalase-negative gram-positive bacterium, is aerotolerant and survives H2O2 exposures that kill many catalase-positive bacteria. The molecular basis of the H2O2 resistance is poorly known. Here, we demonstrate that serotype M49 GAS lacking the Rgg regulator is m...

journal_title：Journal of bacteriology

authors： Pulliainen AT,Hytönen J,Haataja S,Finne J

更新日期：2008-05-01 00:00:00

abstract：:Uptake of (35)S-hydroxyethylthiazole (4-methyl-5-hydroxyethylthiazole) by Escherichia coli intact cells was studied. Hydroxyethylthiazole was taken up in the presence and absence of glucose at the same rate. The uptake was almost proportional to a hydroxyethylthiazole concentration gradient up to 0.1 mM with no tenden...

journal_title：Journal of bacteriology

authors： Yamasaki H,Sanemori H,Yamada K,Kawasaki T

更新日期：1973-12-01 00:00:00

abstract：:We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed th...

journal_title：Journal of bacteriology

authors： Flache P,Baumeister R,Hillen W

更新日期：1992-04-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil-dwelling alphaproteobacterium that engages in a nitrogen-fixing root nodule symbiosis with leguminous plants. Cell surface polysaccharides are important both for adapting to stresses in the soil and for the development of an effective symbiotic interaction. Among the polysaccharides ch...

journal_title：Journal of bacteriology

authors： Barnett MJ,Long SR

更新日期：2018-01-10 00:00:00

abstract：:The halophilic bacterium Chromohalobacter salexigens synthesizes and accumulates compatible solutes in response to salt and temperature stress. (13)C-nuclear magnetic resonance analysis of cells grown in minimal medium at the limiting temperature of 45 degrees C revealed the presence of hydroxyectoine, ectoine, glutam...

journal_title：Journal of bacteriology

authors： García-Estepa R,Argandoña M,Reina-Bueno M,Capote N,Iglesias-Guerra F,Nieto JJ,Vargas C

更新日期：2006-06-01 00:00:00

abstract：:A protein carboxyl methyltransferase (EC 2.1.1.77) that recognizes age-damaged proteins for potential repair or degradation reactions has been found in all vertebrate tissues and cells examined to date. This enzyme catalyzes the transfer of methyl groups from S-adenosylmethionine to the carboxyl groups of D-aspartyl o...

journal_title：Journal of bacteriology

authors： Li C,Clarke S

更新日期：1992-01-01 00:00:00

abstract：:The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally altered cell wall (1----6)-beta-glucan, which results in resistance to K1 killer toxin. We isolated the canKRE1 gene from Ca...

journal_title：Journal of bacteriology

authors： Boone C,Sdicu A,Laroche M,Bussey H

更新日期：1991-11-01 00:00:00

abstract：:Fertility factor F confers bacterial conjugation, a process which involves at least 20 tra genes. Resistance plasmids such as R100, R6-5, and R1 have homology with F in the tra region. Conjugal transfer of these plasmids is, however, repressed, while transfer of F is constitutive. Repression of R transfer is due to th...

journal_title：Journal of bacteriology

authors： Yoshioka Y,Ohtsubo H,Ohtsubo E

更新日期：1987-02-01 00:00:00

abstract：:Helicobacter pylori urease requires nickel ions in the enzyme active site for catalytic activity. Nickel ions must, therefore, be actively acquired by the bacterium. NixA (high-affinity nickel transport protein)-deficient mutants of H. pylori retain significant urease activity, suggesting the presence of alternate nic...

journal_title：Journal of bacteriology

authors： Hendricks JK,Mobley HL

更新日期：1997-09-01 00:00:00

abstract：:During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...

journal_title：Journal of bacteriology

authors： Daborn PJ,Waterfield N,Blight MA,Ffrench-Constant RH

更新日期：2001-10-01 00:00:00

abstract：:Wild-type strains of Escherichia coli K-12 are resistant to acriflavine. Gene acrA(+) which determines resistance to acriflavine is located near the lac region of the chromosome. This gene determines not only resistance to basic dyes but also resistance to phenethyl alcohol. Acriflavine resistance was transmitted, tog...

journal_title：Journal of bacteriology

authors： Nakamura H

更新日期：1968-10-01 00:00:00

abstract：:The par genes of unit-copy plasmids P1 and F promote equitable distribution of plasmid copies to daughter cells and can be considered to be functional analogs of eucaryotic centromeres. Composite plasmids were constructed which carry either two functional P1 par regions or one F and one P1 region. Unlike dicentric chr...

journal_title：Journal of bacteriology

authors： Austin SJ

更新日期：1984-05-01 00:00:00

abstract：:A selection procedure is described which permits a large number of Saccharomyces cerevisiae mutants to be screened for specific lesions in mitochondrial respiratory enzymes and the adenosine triphosphatase. The method has been used to isolate nuclear mutant strains with specific lesions in coenzyme QH2-cytochrome c re...

journal_title：Journal of bacteriology

authors： Tzagoloff A,Akai A,Needleman RB

更新日期：1975-06-01 00:00:00

abstract：:By transformation of dnaA null mutant host cells that are suppressed either by an rnh mutation or by chromosomal integration of a mini-R1 plasmid, it was shown that replication of miniplasmids composed of the NR1 minimal replicon had no absolute dependence upon DnaA protein. In addition, the suppression of the dnaA nu...

journal_title：Journal of bacteriology

authors： Tang XB,Womble DD,Rownd RH

更新日期：1989-10-01 00:00:00

abstract：:Aerosols of Mycoplasma pneumoniae were studied at several relative humidities at a controlled temperature of 27 C. Production of an experimentally reproducible aerosol required preatomization of the organism in its suspending fluid and was dependent on the type of fluid used in atomization as well as on the procedures...

journal_title：Journal of bacteriology

authors： Wright DN,Bailey GD,Hatch MT

更新日期：1968-10-01 00:00:00

abstract：:Plasmids for high-level expression of penicillin-binding protein 6 (PBP6) were constructed, giving rise to overproduction of PBP6 under the control of the lambda pR promoter in either the periplasmic or the cytoplasmic space. In contrast to penicillin-binding protein 5 (PBP5), the presence of high amounts of PBP6 in t...

journal_title：Journal of bacteriology

authors： van der Linden MP,de Haan L,Hoyer MA,Keck W

更新日期：1992-12-01 00:00:00

abstract：:Sigma 54 associates with bacterial core RNA polymerase and converts it into an enhancer-responsive enzyme. Deletion of the N-terminal 40 amino acids is known to result in loss of the ability to respond to enhancer binding proteins. In this work PCR mutagenesis and genetic screens were used to identify a small patch, f...

journal_title：Journal of bacteriology

authors： Syed A,Gralla JD

更新日期：1998-11-01 00:00:00

abstract：:The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P(spac) promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 ...

journal_title：Journal of bacteriology

authors： Pinho MG,Filipe SR,de Lencastre H,Tomasz A

更新日期：2001-11-01 00:00:00

abstract：:Bam35, a temperate double-stranded DNA bacteriophage with a 15-kb linear genome, infects gram-positive Bacillus thuringiensis cells. Bam35 morphology and genome organization resemble those of PRD1, a lytic phage infecting gram-negative bacteria. Bam35 and PRD1 have an outer protein coat surrounding a membrane that enc...

journal_title：Journal of bacteriology

authors： Gaidelyte A,Jaatinen ST,Daugelavicius R,Bamford JK,Bamford DH

更新日期：2005-05-01 00:00:00

abstract：:Four mutants specifically deficient in the activity of isocitrate lyase were independently isolated in the alkane yeast Saccharomycopsis lipolytica. Genetic analysis by means of protoplast fusion and mitotic haploidization revealed that the mutations were recessive and non-complementary at a single genetic locus, icl....

journal_title：Journal of bacteriology

authors： Matsuoka M,Himeno T,Aiba S

更新日期：1984-03-01 00:00:00