Genetic determination of resistance to acriflavine, phenethyl alcohol, and sodium dodecyl sulfate in Escherichia coli.

abstract：:When Bacillus megaterium spores germinate in the absence of an exogenous carbon source, the first minutes of germination are accompanied by production of large amounts (approximately 70 nmol/mg of dry spores) of acetate and much smaller amounts of pyruvate and lactate. The majority of these compounds are excreted into...

journal_title：Journal of bacteriology

authors： Setlow B,Shay LK,Vary JC,Setlow P

更新日期：1977-11-01 00:00:00

abstract：:The mechanism of methyl-beta-D-thiogalactoside-phosphate (TMG-P) expulsion from Streptococcus pyogenes was studied. The expulsion elicited by glucose was not due to exchange vectorial transphosphorylation between the expelled TMG and the incoming glucose since more beta-galactoside was displaced than glucose taken up,...

journal_title：Journal of bacteriology

authors： Reizer J,Novotny MJ,Panos C,Saier MH Jr

更新日期：1983-10-01 00:00:00

abstract：:Nicotinamide adenine dinucleotide (NAD) and reduced NAD (NADH) levels have been measured in bacterial cultures. The cofactors were assayed by using the very sensitive cycling assay described previously by Cartier. Control experiments showed that the level of total NAD(H) falls during harvesting, and so samples were ta...

journal_title：Journal of bacteriology

authors： Wimpenny JW,Firth A

更新日期：1972-07-01 00:00:00

abstract：:Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...

journal_title：Journal of bacteriology

authors： Zagotta MT,Wilson DB

更新日期：1990-02-01 00:00:00

abstract：:Pasteurella multocida is the causative agent of a range of diseases with economic importance in production animals. Many systems have been employed to identify virulence factors of P. multocida, including in vivo expression technology (IVET), signature-tagged mutagenesis, and whole-genome expression profiling. In a pr...

journal_title：Journal of bacteriology

authors： Boucher DJ,Adler B,Boyce JD

更新日期：2005-04-01 00:00:00

abstract：:In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...

journal_title：Journal of bacteriology

authors： Matsuyama T,Bhasin A,Harshey RM

更新日期：1995-02-01 00:00:00

abstract：:Five clostridial species were found to ferment gluconate via 2-keto-3-deoxygluconate which subsequently is phosphorylated to yield 2-keto-3-deoxy-6-phosphogluconate (KDPG). This compound is then cleaved by KDPG aldolase. ...

journal_title：Journal of bacteriology

authors： Bender R,Andreesen JR,Gottschalk G

更新日期：1971-08-01 00:00:00

abstract：:We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and al...

journal_title：Journal of bacteriology

authors： Ou JT,Anderson TF

更新日期：1970-06-01 00:00:00

abstract：:Pseudomonas aeruginosa isolates from chronic lung infections often overproduce alginate, giving rise to the mucoid phenotype. Isolation of mucoid strains from chronic lung infections correlates with a poor patient outcome. The most common mutation that causes the mucoid phenotype is called mucA22 and results in a trun...

journal_title：Journal of bacteriology

authors： Cross AR,Raghuram V,Wang Z,Dey D,Goldberg JB

更新日期：2020-09-23 00:00:00

abstract：:CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...

journal_title：Journal of bacteriology

authors： Heilpern AJ,Waldor MK

更新日期：2003-02-01 00:00:00

abstract：:Listeria monocytogenes is a facultative intracellular gram-positive bacterium responsible for severe opportunistic infections in humans and animals. We had previously identified a gene encoding a putative UDP-N-acetylglucosamine 2-epimerase, a precursor of the teichoic acid linkage unit, in the genome of L monocytogen...

journal_title：Journal of bacteriology

authors： Dubail I,Bigot A,Lazarevic V,Soldo B,Euphrasie D,Dupuis M,Charbit A

更新日期：2006-09-01 00:00:00

abstract：:Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

journal_title：Journal of bacteriology

authors： Milunovic B,diCenzo GC,Morton RA,Finan TM

更新日期：2014-02-01 00:00:00

abstract：:Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...

journal_title：Journal of bacteriology

authors： Römermann D,Friedrich B

更新日期：1985-05-01 00:00:00

abstract：:A sucrose-inducible alpha-glucosidase activity that hydrolyzes sucrose in Candida albicans has been demonstrated previously. The enzyme is assayable in whole cells and was inhibited by both sucrose and maltose. A C. albicans gene (CASUC1) that affects sucrose utilization and alpha-glucosidase activity was cloned by ex...

journal_title：Journal of bacteriology

authors： Kelly R,Kwon-Chung KJ

更新日期：1992-01-01 00:00:00

abstract：:Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance w...

journal_title：Journal of bacteriology

authors： Zheng G,Yan LZ,Vederas JC,Zuber P

更新日期：1999-12-01 00:00:00

abstract：:The class 1 outer membrane protein encoded by the porA gene of Neisseria meningitidis is a candidate for a vaccine against meningococcal infection. The expression of class 1 outer membrane protein displays phase variation between three expression levels. Northern (RNA) blot and primer extension analysis revealed that ...

journal_title：Journal of bacteriology

authors： van der Ende A,Hopman CT,Zaat S,Essink BB,Berkhout B,Dankert J

更新日期：1995-05-01 00:00:00

abstract：:Physiological studies of a mutant of Escherichia coli lacking the three lytic transglycosylases Slt70, MltA, and MltB revealed that interference with murein turnover can prevent AmpC beta-lactamase induction. The triple mutant, although growing normally, shows a dramatically reduced rate of murein turnover. Despite th...

journal_title：Journal of bacteriology

authors： Kraft AR,Prabhu J,Ursinus A,Höltje JV

更新日期：1999-12-01 00:00:00

abstract：:Bacillus anthracis, the causative agent of anthrax, replicates as chains of vegetative cells by regulating the separation of septal peptidoglycan. Surface (S)-layer proteins and associated proteins (BSLs) function as chain length determinants and bind to the secondary cell wall polysaccharide (SCWP). In this study, we...

journal_title：Journal of bacteriology

authors： Liszewski Zilla M,Chan YG,Lunderberg JM,Schneewind O,Missiakas D

更新日期：2015-01-01 00:00:00

abstract：:Daniel, John W. (University of Wisconsin, Madison), and Karlee Babcock. Methionine metabolism of the myxomycete Physarum polycephalum. J. Bacteriol. 92:1028-1035. 1966.-Previous studies have shown that Physarum polycephalum requires exogenous methionine for growth, but not cysteine, folic acid, or vitamin B(12). Methi...

journal_title：Journal of bacteriology

authors： Daniel JW,Babcock K

更新日期：1966-10-01 00:00:00

abstract：:Peptide deformylase (PDF) is an essential bacterial metalloprotease involved in deformylation of N-formyl group from nascent polypeptide chains during protein synthesis. Iron-containing variants of this enzyme from Salmonella enterica serovar Typhimurium (sPDF) and Mycobacterium tuberculosis (mPDF), although inhibited...

journal_title：Journal of bacteriology

authors： Kumar S,Kanudia P,Karthikeyan S,Chakraborti PK

更新日期：2014-01-01 00:00:00

abstract：:Acclimation to high salt concentrations involves concerted changes in gene expression. For the majority of salt-regulated genes, the mechanism underlying the induction process is not known. The gene ggpS (sll1566), which encodes the glucosylglycerol-phosphate synthase responsible for the synthesis of the compatible so...

journal_title：Journal of bacteriology

authors： Klähn S,Höhne A,Simon E,Hagemann M

更新日期：2010-09-01 00:00:00

abstract：:Tn5 was used to generate mutants that were deficient in the dissimilatory reduction of nitrite for Pseudomonas sp. strain G-179, which contains a copper nitrite reductase. Three types of mutants were isolated. The first type showed a lack of growth on nitrate, nitrite, and nitrous oxide. The second type grew on nitrat...

journal_title：Journal of bacteriology

authors： Ye RW,Averill BA,Tiedje JM

更新日期：1992-10-01 00:00:00

abstract：:spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...

journal_title：Journal of bacteriology

authors： Siranosian KJ,Grossman AD

更新日期：1994-06-01 00:00:00

abstract：:An Escherichia coli F19 recA, nitrate reductase-deficient mutant was constructed by transposon mutagenesis and shown to be resistant to metronidazole. This mutant was a most suitable host for the isolation of Clostridium acetobutylicum genes on recombinant plasmids, which activated metronidazole and rendered the E. co...

journal_title：Journal of bacteriology

authors： Santangelo JD,Jones DT,Woods DR

更新日期：1991-02-01 00:00:00

abstract：:Polyamines are present in high concentrations in archaea, yet little is known about their synthesis, except by extrapolation from bacterial and eucaryal systems. S-Adenosylmethionine (AdoMet) decarboxylase, a pyruvoyl group-containing enzyme that is required for spermidine biosynthesis, has been previously identified ...

journal_title：Journal of bacteriology

authors： Kim AD,Graham DE,Seeholzer SH,Markham GD

更新日期：2000-12-01 00:00:00

abstract：:Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...

journal_title：Journal of bacteriology

authors： Vales LD,Chase JW,Murphy JB

更新日期：1979-07-01 00:00:00

abstract：:Genetically disabled lambda plac5 transducing phage derivatives were used to study the recB dependence of recombination during specialized transduction. The frequency of transduction was normalized to colony-forming units, and the end product of recombination was monitored by scoring for addition and substitution tran...

journal_title：Journal of bacteriology

authors： Porter RD,Welliver RA,Witkowski TA

更新日期：1982-06-01 00:00:00

abstract：:Chlamydia trachomatis is a human bacterial pathogen that multiplies only within an intracellular membrane-bound vacuole, the inclusion. C. trachomatis includes ocular and urogenital strains, usually causing infections restricted to epithelial cells of the conjunctiva and genital mucosa, respectively, and lymphogranulo...

journal_title：Journal of bacteriology

authors： Almeida F,Borges V,Ferreira R,Borrego MJ,Gomes JP,Mota LJ

更新日期：2012-12-01 00:00:00

abstract：:A mutant of Escherichia coli, previously shown to contain abnormal nucleoside triphosphate pools, was found to be defective in its ability to synthesize thymidine nucleotides. The defect is not in the enzyme thymidylate synthetase but in deoxycytidine triphosphate deaminase, an enzyme that supplies deoxyuridine monoph...

journal_title：Journal of bacteriology

authors： O'Donovan GA,Edlin G,Fuchs JA,Neuhard J,Thomassen E

更新日期：1971-02-01 00:00:00

abstract：:DNA sequence analysis of the modABCD operon of Escherichia coli revealed the presence of four open reading frames. The first gene, modA, codes for a 257-amino-acid periplasmic binding protein enunciated by the presence of a signal peptide-like sequence. The second gene (modB) encodes a 229-amino-acid protein with a po...

journal_title：Journal of bacteriology

authors： Maupin-Furlow JA,Rosentel JK,Lee JH,Deppenmeier U,Gunsalus RP,Shanmugam KT

更新日期：1995-09-01 00:00:00