Activation of spo0A transcription by sigma H is necessary for sporulation but not for competence in Bacillus subtilis.

abstract：:Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...

journal_title：Journal of bacteriology

authors： Kovacs SA,O'Neil J,Watcharapijarn J,Moe-Kirvan C,Vijay S,Silva V

更新日期：1993-04-01 00:00:00

abstract：:The phosphomannose isomerase (pmi) gene of Escherichia coli was cloned on a broad-host-range cosmid vector and expressed in Pseudomonas aeruginosa at a low level. Plasmid pAD3, which harbors the E. coli pmi gene, contains a 6.2-kilobase-pair HindIII fragment derived from the chromosome of E. coli. Subcloning produced ...

journal_title：Journal of bacteriology

authors： Darzins A,Nixon LL,Vanags RI,Chakrabarty AM

更新日期：1985-01-01 00:00:00

abstract：:Ureaplasma urealyticum cells were lysed by osmotic shock or by digitonin. The membrane fraction contained four to ten times as much protein as the cytoplasmic fraction. These values are in large excess of those reported for classical mycoplasmas, suggesting that the Ureaplasma membrane fraction was heavily contaminate...

journal_title：Journal of bacteriology

authors： Masover GK,Razin S,Hayflick L

更新日期：1977-04-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter capsulatus contains two [NiFe]hydrogenases: an energy-generating hydrogenase, HupSL, and a regulatory hydrogenase, HupUV. The synthesis of HupSL is specifically activated by H(2) through a signal transduction cascade comprising three proteins: the H(2)-sensing HupUV protein, th...

journal_title：Journal of bacteriology

authors： Elsen S,Duché O,Colbeau A

更新日期：2003-12-01 00:00:00

abstract：:Rhodobacter sphaeroides cells containing an in-frame deletion within ccmA lack detectable soluble and membrane-bound c-type cytochromes and are unable to grow under conditions where these proteins are required. Only strains merodiploid for ccmABCDG were found after attempting to generate cells containing either a ccmG...

journal_title：Journal of bacteriology

authors： Cox RL,Patterson C,Donohue TJ

更新日期：2001-08-01 00:00:00

abstract：:Rabbits immunized with mucopeptide derived from cell wall debris of Streptococcus agalactiae by use of the formamide extraction technique developed specific antibodies for bovine heart, skeletal muscle, lymph nodes, and blood buffy coat extracts. ...

journal_title：Journal of bacteriology

authors： Dodd K,Norcross NL

更新日期：1967-02-01 00:00:00

abstract：:Transcription of the archaeal trkA gene homolog in Methanosarcina mazei S-6 was studied at the optimal growth temperature of 37 degrees C and after heat shock at 45 degrees C. Northern (RNA) blotting results (transcript size) and data from primer extension experiments to map the transcription initiation site indicate ...

journal_title：Journal of bacteriology

authors： Conway de Macario E,Macario AJ

更新日期：1995-11-01 00:00:00

abstract：:Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...

journal_title：Journal of bacteriology

authors： Ruppert C,Wimmers S,Lemker T,Müller V

更新日期：1998-07-01 00:00:00

abstract：:The genes for the beta, beta', and seven sigma factor subunits of RNA polymerase, for elongation factors EF-Tu1 and EF-Tu3, and for six rRNA operons were mapped on the combined genetic and physical map of the Streptomyces coelicolor chromosome. Like the previously mapped tRNA genes, the RNA polymerase and rRNA genes m...

journal_title：Journal of bacteriology

authors： van Wezel GP,Buttner MJ,Vijgenboom E,Bosch L,Hopwood DA,Kieser HM

更新日期：1995-01-01 00:00:00

abstract：:We have used Tn917lacZ to mutagenize the Bacillus subtilis chromosome and have isolated mutants that are defective in chemotaxis and motility. Mapping of the transposon inserts identified two new loci. Mutations in one of these loci generated mutants that had paralyzed flagella. Accordingly, we designate this a mot lo...

journal_title：Journal of bacteriology

authors： Zuberi AR,Ying CW,Parker HM,Ordal GW

更新日期：1990-12-01 00:00:00

abstract：:A major phenotypic trait of the Mycobacterium avium complex is the ability to produce rough and smooth colony variants. The chemical basis of this morphological variation is the loss of an antigenic surface structure, termed glycopeptidolipid (GPL), by rough variants. Using M. avium serovar 2 strain 2151 as a model sy...

journal_title：Journal of bacteriology

authors： Eckstein TM,Inamine JM,Lambert ML,Belisle JT

更新日期：2000-11-01 00:00:00

abstract：:The glnB gene from Bradyrhizobium japonicum, the endosymbiont of soybeans (Glycine max), was isolated and sequenced, and its expression was examined under various culture conditions and in soybean nodules. The B. japonicum glnB gene encodes a 12,237-dalton polypeptide that is highly homologous to the glnB gene product...

journal_title：Journal of bacteriology

authors： Martin GB,Thomashow MF,Chelm BK

更新日期：1989-10-01 00:00:00

abstract：:An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 was used to study genetic deletions between directly repeated sequences. The frequency of deletion was highest under conditions in which the DNA was actively replicating. Deletion frequency increased markedly with the length of the...

journal_title：Journal of bacteriology

authors： Kong D,Masker W

更新日期：1994-10-01 00:00:00

abstract：:In Escherichia coli K-12, the rcsA and rcsB gene products are positive regulators in expression of the slime polysaccharide colanic acid. We have previously demonstrated the presence of rcsA sequences in E. coli K1 and K5, strains with group II capsular K antigens, and shown that introduction of multicopy rcsA into th...

journal_title：Journal of bacteriology

authors： Keenleyside WJ,Bronner D,Jann K,Jann B,Whitfield C

更新日期：1993-10-01 00:00:00

abstract：:Forty-nine human and environmental isolates of Mycobacterium intracellulare and Mycobacterium scrofulaceum were tested for their ability to grow on uric acid and a number of its degradation products. Nearly all (88 to 90%) strains used uric acid or allantoin as a sole nitrogen source; fewer (47 to 69%) used allantoate...

journal_title：Journal of bacteriology

authors： Falkinham JO 3rd,George KL,Parker BC,Gruft H

更新日期：1983-07-01 00:00:00

abstract：:Cells of Candida albicans WO-1 switch frequently and reversibly between two colony-forming phenotypes, white and opaque. In the white form, budding cells appear similar to those of most other strains of C. albicans, but in the opaque form, budding cells are larger, are bean shaped, and possess pimples on the wall. The...

journal_title：Journal of bacteriology

authors： Anderson J,Mihalik R,Soll DR

更新日期：1990-01-01 00:00:00

abstract：:A globomycin-resistant mutant of Escherichia coli was found to produce a precursor of the major outer membrane lipoprotein (prolipoprotein), in which the glycine residue at position 14 within the signal peptide was replaced by an aspartic acid residue. The same mutation has been reported by Lin et al. (Proc. Natl. Aca...

journal_title：Journal of bacteriology

authors： Lee N,Yamagata H,Inouye M

更新日期：1983-07-01 00:00:00

abstract：:Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and alpha-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thu...

journal_title：Journal of bacteriology

authors： McCullough J,Herskowitz I

更新日期：1979-04-01 00:00:00

abstract：:The whole-genome sequence of Mycobacterium bolletii M24, isolated from the bronchoalveolar lavage fluid of a Malaysian patient, is reported here. The circular chromosome of 5,507,730 bp helped to clarify the taxonomic position of this organism within the M. abscessus complex and revealed the presence of proteins poten...

journal_title：Journal of bacteriology

authors： Wong YL,Choo SW,Tan JL,Ong CS,Ng KP,Ngeow YF

更新日期：2012-08-01 00:00:00

abstract：:The role of the proton gradient and external pH in the motility and chemotaxis of Bacillus subtilis was investigated. Presence of a substantial proton gradient is not necessary for motility or chemotaxis, as long as the electrical potential is sufficient to maintain motility. Changes in the proton gradient do, however...

journal_title：Journal of bacteriology

authors： Miller JB,Koshland DE Jr

更新日期：1980-01-01 00:00:00

abstract：:Specific changes in the chemical and microbial composition of Thermoanaerobium brockii fermentations were compared and related to alterations of process rates, end product yields, and growth parameters. Fermentation of starch as compared with glucose was associated with significant decreases in growth rate and intrace...

journal_title：Journal of bacteriology

authors： Ben-Bassat A,Lamed R,Zeikus JG

更新日期：1981-04-01 00:00:00

abstract：:The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...

journal_title：Journal of bacteriology

authors： Ottemann KM,DiRita VJ,Mekalanos JJ

更新日期：1992-11-01 00:00:00

abstract：:Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...

journal_title：Journal of bacteriology

authors： Serrano M,Vieira F,Moran CP Jr,Henriques AO

更新日期：2008-12-01 00:00:00

abstract：:Bacteroides fragilis synthesizes eight distinct capsular polysaccharides, more than any described bacterium outside the order Bacteroidales. Here, we show that this organism also produces a high-molecular-weight extracellular polysaccharide (EPS). Expression of the EPS results in the formation of a large polysaccharid...

journal_title：Journal of bacteriology

authors： Chatzidaki-Livanis M,Coyne MJ,Roche-Hakansson H,Comstock LE

更新日期：2008-02-01 00:00:00

abstract：:Spores of all strains of Dictyostelium discoideum tested in this study germinated after a heat shock of 45 C for 30 min. Whereas the strains differed in their rates of germination, the rate for each strain was constant. A correlation existed between the rate of germination and the rate of vegetative growth when spores...

journal_title：Journal of bacteriology

authors： Cotter DA,Raper KB

更新日期：1968-11-01 00:00:00

abstract：:A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...

journal_title：Journal of bacteriology

authors： Day JM,Janssen GR

更新日期：2004-10-01 00:00:00

abstract：:Rod-shaped bacteria grow by a repetitive cycle of elongation followed by division, and the mechanisms responsible for these two processes have been studied for decades. However, little is known about what happens during the transition between the two activities. At least one event occurs after elongation ends and befo...

journal_title：Journal of bacteriology

authors： Potluri LP,Kannan S,Young KD

更新日期：2012-10-01 00:00:00

abstract：:Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for gluc...

journal_title：Journal of bacteriology

authors： Gumerov VM,Mardanov AV,Beletsky AV,Prokofeva MI,Bonch-Osmolovskaya EA,Ravin NV,Skryabin KG

更新日期：2011-05-01 00:00:00

abstract：:Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate...

journal_title：Journal of bacteriology

authors： KONDO E,MITSUHASHI S

更新日期：1964-11-01 00:00:00

abstract：:Escherichia coli O55 is an important antigen which is often associated with enteropathogenic E. coli clones. We sequenced the genes responsible for its synthesis and identified genes for O-antigen polymerase, O-antigen flippase, four enzymes involved in GDP-colitose synthesis, and three glycosyltransferases, all by co...

journal_title：Journal of bacteriology

authors： Wang L,Huskic S,Cisterne A,Rothemund D,Reeves PR

更新日期：2002-05-01 00:00:00