Abstract:
:Specific changes in the chemical and microbial composition of Thermoanaerobium brockii fermentations were compared and related to alterations of process rates, end product yields, and growth parameters. Fermentation of starch as compared with glucose was associated with significant decreases in growth rate and intracellular fructose-1,6-bisphosphate concentration and with a dramatic increase in the ethanol/lactate product ratio. Glucose or pyruvate fermentation in the presence of acetone was correlated with increased substrate consumption, growth (both rate and yield), acetate yield, and quantitative reduction of acetone to isopropanol in lieu of normal reduced fermentation products (i.e., H2, ethanol, lactate). Acetone altered pyruvate phosphoroclastic activity of cell extracts in that H2, lactate, and ethanol levels decreased, whereas the acetate concentration increased. Glucose fermentation in the presence of exogenous hydrogen was associated with inhibition of endogenous H2 production and either increased ethanol/acetate product ratios and decreased growth at less than 0.5 atm (51 kPa) of H2 or total growth inhibition at 1.0 atm (102 kPA). The effects of exogenous hydrogen on glucose fermentation were totally reversed by the addition of acetone. Glucose fermentation in coculture with Methanobacterium thermoautotrophicum correlated with increased growth (both rate and yield), acetate yield, and the formation of methane in lieu of monoculture reduced products. In coculture, but not monoculture, T. brockii grew on ethanol as the energy source, and acetate and methane were the end products as a direct consequence of hydrogen consumption by the methanogen.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ben-Bassat A,Lamed R,Zeikus JGdoi
10.1128/JB.146.1.192-199.1981subject
Has Abstractpub_date
1981-04-01 00:00:00pages
192-9issue
1eissn
0021-9193issn
1098-5530journal_volume
146pub_type
杂志文章abstract::From the cell envelope preparation of Sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. The high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipop...
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journal_title:Journal of bacteriology
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doi:10.1128/JB.132.2.411-418.1977
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更新日期:1998-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.3.1380-1386.1974
更新日期:1974-12-01 00:00:00
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doi:10.1128/jb.171.6.2919-2924.1989
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更新日期:1996-10-01 00:00:00
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更新日期:1991-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1967-11-01 00:00:00
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更新日期:2004-10-01 00:00:00