Use of the beta-lactamase inhibitor clavulanic acid in the isolation of auxotrophic mutants of Bacillus licheniformis.

abstract：:Sigma 54 associates with bacterial core RNA polymerase and converts it into an enhancer-responsive enzyme. Deletion of the N-terminal 40 amino acids is known to result in loss of the ability to respond to enhancer binding proteins. In this work PCR mutagenesis and genetic screens were used to identify a small patch, f...

journal_title：Journal of bacteriology

authors： Syed A,Gralla JD

更新日期：1998-11-01 00:00:00

abstract：:The mutualistic symbiont Vibrio fischeri builds a symbiotic biofilm during colonization of squid hosts. Regulation of the exopolysaccharide component, termed Syp, has been examined in strain ES114, where production is controlled by a phosphorelay that includes the inner membrane hybrid histidine kinase RscS. Most stra...

journal_title：Journal of bacteriology

authors： Rotman ER,Bultman KM,Brooks JF 2nd,Gyllborg MC,Burgos HL,Wollenberg MS,Mandel MJ

更新日期：2019-04-09 00:00:00

abstract：:The mecA-27r gene from Staphylococcus aureus 27r encodes penicillin-binding protein 2a (PBP2a-27r), which causes this strain to be methicillin resistant. Removal or replacement of the N-terminal transmembrane domain had no effect on binding of penicillin, but removal of portions of the putative transglycosylase domain...

journal_title：Journal of bacteriology

authors： Wu CY,Alborn WE Jr,Flokowitsch JE,Hoskins J,Unal S,Blaszczak LC,Preston DA,Skatrud PL

更新日期：1994-01-01 00:00:00

abstract：:A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1...

journal_title：Journal of bacteriology

authors： Elseviers D,Gallagher P,Hoffman A,Weinberg B,Schwartz I

更新日期：1982-10-01 00:00:00

abstract：:Saccharopolyspora spinosa is a Gram-positive bacterium that produces spinosad, a well-known biodegradable insecticide that is used for agricultural pest control and has an excellent environmental and mammalian toxicological profile. Here, we present the first draft genome sequence of the type strain Saccharopolyspora ...

journal_title：Journal of bacteriology

authors： Pan Y,Yang X,Li J,Zhang R,Hu Y,Zhou Y,Wang J,Zhu B

更新日期：2011-06-01 00:00:00

abstract：:The ultrastructure of the cytoplasmic membrane and cell wall of two strains of Escherichia coli, Proteus morganii, P. vulgaris, Acinetobacter anitratum, Moraxella lacunata, Erwinia amylovora, Acinetobacter sp., and of a plant pathogen, unclassified gram-negative, fixed by the Ryter-Kellenberger procedure, was found to...

journal_title：Journal of bacteriology

authors： Silva MT,Sousa JC

更新日期：1973-02-01 00:00:00

abstract：:THREE PREVIOUSLY STUDIED R FACTORS WERE USED: 222/R4, controlling transmissible resistance to sulfonamide, streptomycin, chloromycetin, and tetracycline (SU(r) SM(r) CM(r) TC(r)); 222/R3, a derivative of 222/R4 (now termed 222/R3W) having lost TC(r); and R15, controlling infectious resistance to SU and SM only. Two ty...

journal_title：Journal of bacteriology

authors： Nisioka T,Mitani M,Clowes RC

更新日期：1970-07-01 00:00:00

abstract：:The spoVM gene encodes a 26-amino-acid polypeptide that is essential for spore formation in Bacillus subtilis. A transposon insertion within the spoVM open reading frame has been shown to encode a chimeric protein which is biologically inactive and produces a phenotype identical to that of a deletion and insertion mut...

journal_title：Journal of bacteriology

authors： Cutting S,Anderson M,Lysenko E,Page A,Tomoyasu T,Tatematsu K,Tatsuta T,Kroos L,Ogura T

更新日期：1997-09-01 00:00:00

abstract：:We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intracellular and extra...

journal_title：Journal of bacteriology

authors： Calvin NM,Hanawalt PC

更新日期：1988-06-01 00:00:00

abstract：:Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in the filaments were regularly distributed and septum formation was perfectly inhibited. This inhibition of cell division ...

journal_title：Journal of bacteriology

authors： Utsumi R,Tanabe H,Nakamoto Y,Kawamukai M,Sakai H,Himeno M,Komano T,Hirota Y

更新日期：1981-09-01 00:00:00

abstract：:A Bacillus subtilis secA mutant, secA12, which is blocked at an early stage of sporulation, is able to grow as well as the wild-type strain at all temperatures tested. Experiments with lacZ fusion genes showed that the induction of kinA expression, as well as the sporulation-specific transcription of the spo0A gene, w...

journal_title：Journal of bacteriology

authors： Asai K,Kawamura F,Sadaie Y,Takahashi H

更新日期：1997-01-01 00:00:00

abstract：:Phosphatidyl ethanolamine and lipopolysaccharide were extracted and purified from the cell envelope fractions of Escherichia coli and Salmonella typhimurium. The two components were studied separately and after recombination, by use of electron microscopy and monolayer techniques, and by measuring their ability to par...

journal_title：Journal of bacteriology

authors： Rothfield L,Horne RW

更新日期：1967-05-01 00:00:00

abstract：:Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilizatio...

journal_title：Journal of bacteriology

authors： Joshua CJ,Dahl R,Benke PI,Keasling JD

更新日期：2011-03-01 00:00:00

abstract：:The deduced amino acid sequences of the flagellins of Pseudomonas syringae pv. tabaci and P. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. The reason for the difference seems to depend on the posttranslational modification of the flagellins. To...

journal_title：Journal of bacteriology

authors： Takeuchi K,Taguchi F,Inagaki Y,Toyoda K,Shiraishi T,Ichinose Y

更新日期：2003-11-01 00:00:00

abstract：:The recF gene of Escherichia coli is known to encode an Mr-40,000 protein that is involved in DNA recombinationa nd postreplication DNA repair. To characterize the role of the recF gene product in these processes, the recF gene was cloned downstream of a tac promoter to facilitate overproduction of the recF gene produ...

journal_title：Journal of bacteriology

authors： Griffin TJ 4th,Kolodner RD

更新日期：1990-11-01 00:00:00

abstract：:Pep 5 and nisin are cationic peptide antibiotics which in addition to their membrane-disruptive action induce autolysis in staphylococci. To investigate the mechanism of lysis induction, the influence of the peptides on the activity of the N-acetylmuramoyl-L-alanine amidase of Staphylococcus simulans 22 was studied. I...

journal_title：Journal of bacteriology

authors： Bierbaum G,Sahl HG

更新日期：1987-12-01 00:00:00

abstract：:Freeze-etching was applied to preparations, with and without glycerol, of Acinetobacter sp. strain MJT/F5/199A, consisting of intact cells after normal growth or after incubation with chloramphenicol, spheroplasts, and isolated cell walls and outer membranes. Etched preparations show that a regular array of subunits f...

journal_title：Journal of bacteriology

authors： Sleytr UB,Thornley MJ

更新日期：1973-12-01 00:00:00

abstract：:Streptomyces coelicolor is the prototype for the investigation of antibiotic-producing and differentiating actinomycetes. As soil bacteria, streptomycetes can metabolize a wide variety of carbon sources and are hence vested with various specific permeases. Their activity and regulation substantially determine the nutr...

journal_title：Journal of bacteriology

authors： Bertram R,Schlicht M,Mahr K,Nothaft H,Saier MH Jr,Titgemeyer F

更新日期：2004-03-01 00:00:00

abstract：:Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...

journal_title：Journal of bacteriology

authors： Bladen HA,Hampp EG

更新日期：1964-05-01 00:00:00

abstract：:Bacillus subtilis undergoes a highly distinctive division during spore formation. It yields two unequal cells, the mother cell and the prespore, and septum formation is completed before the origin-distal 70% of the chromosome has entered the smaller prespore. The mother cell subsequently engulfs the prespore. Two diff...

journal_title：Journal of bacteriology

authors： Bogush M,Xenopoulos P,Piggot PJ

更新日期：2007-05-01 00:00:00

abstract：:Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Joseph L. Melnick, and Fred Rapp. Effects of pancreatin on the growth of reovirus. J. Bacteriol. 92:155-160. 1966.-The influence of pancreatin and other proteolytic enzymes on the growth, plaque formation, and antigenicity of reovirus was studied. S...

journal_title：Journal of bacteriology

authors： Wallis C,Melnick JL,Rapp F

更新日期：1966-07-01 00:00:00

abstract：:Competent cell-deoxyribonucleic acid (DNA) interactions were examined using tritium-labeled homologous or heterologous native or denatured DNAs and competent Streptococcus sanguis Wicky cells (strain WE4). The DNAs used were extracted from WE4 cells, Escherichia coli B cells, and E. coli bacteriophages T2, T4, T6, and...

journal_title：Journal of bacteriology

authors： Ranhand JM

更新日期：1980-05-01 00:00:00

abstract：:Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share ...

journal_title：Journal of bacteriology

authors： Sutcliffe J,Blumenthal R,Walter A,Foulds J

更新日期：1983-11-01 00:00:00

abstract：:The induction of paraffin oxidation in intact cells of Pseudomonas aeruginosa was investigated. Oxidation of (14)C-heptane by cell-free extracts of adapted cells showed that the activity of whole cells is a reliable reflection of the synthesis of the first enzyme in the degradation of n-alkanes. Induction was signific...

journal_title：Journal of bacteriology

authors： van Eyk J,Bartels TJ

更新日期：1968-09-01 00:00:00

abstract：:In earlier studies from this laboratory, Xanthomonas campestris pv. glycines was found to exhibit a nutrition stress-related postexponential rapid cell death (RCD). The RCD was exhibited in protein-rich media but not in starch or other minimal media. This RCD in X. campestris pv. glycines was found to display features...

journal_title：Journal of bacteriology

authors： Raju KK,Gautam S,Sharma A

更新日期：2006-08-01 00:00:00

abstract：:Mitospores or cysts of Allomyces macrogynus do not take up the glucose analogue 2-deoxyglucose. Uptake of 2-deoxyglucose by germlings begins at 25 min into germination, the start of the rhizoid stage, and increases in rate by approximately 50-fold until 100 min into germination. The rate remains constant from 100 to 2...

journal_title：Journal of bacteriology

authors： Burke DD

更新日期：1975-11-01 00:00:00

abstract：:Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for gluc...

journal_title：Journal of bacteriology

authors： Gumerov VM,Mardanov AV,Beletsky AV,Prokofeva MI,Bonch-Osmolovskaya EA,Ravin NV,Skryabin KG

更新日期：2011-05-01 00:00:00

abstract：:Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

journal_title：Journal of bacteriology

authors： Milunovic B,diCenzo GC,Morton RA,Finan TM

更新日期：2014-02-01 00:00:00

abstract：:Two sets of genes for the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) were detected in the photosynthetic purple sulfur bacterium Chromatium vinosum by hybridization analysis with RuBisCO gene probes, cloned by using the lambda Fix vector, and designated rbcL-rbcS and rbcA-rbc...

journal_title：Journal of bacteriology

authors： Viale AM,Kobayashi H,Akazawa T

更新日期：1989-05-01 00:00:00

abstract：:During colonization of the alfalfa rhizosphere, Pseudomonas fluorescens F113 undergoes phenotypic variation, resulting in the appearance of colonies with different morphology. Among phenotypic variants, three isolates, C, F, and S were selected, with the C variant showing colony morphology identical to that of the ino...

journal_title：Journal of bacteriology

authors： Sánchez-Contreras M,Martín M,Villacieros M,O'Gara F,Bonilla I,Rivilla R

更新日期：2002-03-01 00:00:00