Abstract:
:The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is one of the few glycosylated proteins found in Escherichia coli. Glycosylation is mediated by a specific heptosyltransferase encoded by the aah gene, but little is known about the role of this modification and the mechanism involved. In this study, we identified several peptides of AIDA-I modified by the addition of heptoses by use of mass spectrometry and N-terminal sequencing of proteolytic fragments of AIDA-I. One threonine and 15 serine residues were identified as bearing heptoses, thus demonstrating for the first time that AIDA-I is O-glycosylated. We observed that unglycosylated AIDA-I is expressed in smaller amounts than its glycosylated counterpart and shows extensive signs of degradation upon heat extraction. We also observed that unglycosylated AIDA-I is more sensitive to proteases and induces important extracytoplasmic stress. Lastly, as was previously shown, we noted that glycosylation is required for AIDA-I to mediate adhesion to cultured epithelial cells, but purified mature AIDA-I fused to GST was found to bind in vitro to cells whether or not it was glycosylated. Taken together, our results suggest that glycosylation is required to ensure a normal conformation of AIDA-I and may be only indirectly necessary for its cell-binding function.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Charbonneau ME,Girard V,Nikolakakis A,Campos M,Berthiaume F,Dumas F,Lépine F,Mourez Mdoi
10.1128/JB.00969-07subject
Has Abstractpub_date
2007-12-01 00:00:00pages
8880-9issue
24eissn
0021-9193issn
1098-5530pii
JB.00969-07journal_volume
189pub_type
杂志文章abstract::Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma, four less abundant species, and several minor species, alpha, beta, and gamma make up about 18, 18 and 36%, respectively, o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.3.972-982.1981
更新日期:1981-06-01 00:00:00
abstract::rpoS, a gene that encodes an alternative sigma factor (also known as katF), is critical for the ability of Yersinia enterocolitica grown at 37 degrees C, but not at 26 degrees C, to survive diverse environmental insults such as high temperature, hydrogen peroxide, osmolarity, and low pH. However, a Y. enterocolitica r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.18.5370-5373.1995
更新日期:1995-09-01 00:00:00
abstract::The characteristics of a system for the in vitro synthesis of mitochondrial deoxyribonucleic acid (mtDNA) in mitochondria isolated from Saccharomyces cerevisiae are described. In this system the exclusive product of the reaction is mtDNA. Under optimal conditions the initial rate of synthesis is close to the calculate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.973-982.1977
更新日期:1977-06-01 00:00:00
abstract::The kinetics of chromosome replication and cell division have been examined in recA mutants of Escherichia coli B/r containing F' plasmids of various sizes. Plasmid-mediated alterations in growth properties were detected only with the presence of the larger F' plasmids, and were reflected in decreased mean cell sizes ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1151-1157.1979
更新日期:1979-03-01 00:00:00
abstract::d-Desthiobiotin synthetase, an enzyme that catalyzes the synthesis of d-desthiobiotin from dl-7,8-diaminopelargonic acid and HCO(3) (-), was purified 100-fold from cells of a biotin mutant strain of Escherichia coli. Adenosine triphosphate and Mg(2+) were shown, especially in purified extracts, to be obligatory for en...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.2.726-733.1970
更新日期:1970-11-01 00:00:00
abstract::A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.512-521.1983
更新日期:1983-08-01 00:00:00
abstract::We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner. Unlike what is found for other paraquat-inducible genes, high concentrations of paraquat (200 microM) were required to increase the level of hmp expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.10.3164-3170.1997
更新日期:1997-05-01 00:00:00
abstract::A nicotinamide adenine dinucleotide-specific L-(+)-lactate dehydrogenase (LDH) (EC 1.11.27) from Actinomyces viscosus T-6-1600 was purified approximately 110-fold by a combination of diethylaminoethyl-cellulose and 0.5 M Agarose A column chromatography. The ldh was stable at 26 C, but was quite labile at temperatures ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.1126-1135.1975
更新日期:1975-06-01 00:00:00
abstract::We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.12.3447-3456.1996
更新日期:1996-06-01 00:00:00
abstract::Rhodococcus equi is a facultative intracellular opportunistic pathogen of immunocompromised people and a major cause of pneumonia in young horses. An effective live attenuated vaccine would be extremely useful in the prevention of R. equi disease in horses. Toward that end, we have developed an efficient transposon mu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.8.2644-2652.2003
更新日期:2003-04-01 00:00:00
abstract::Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.8.2179-2183.2000
更新日期:2000-04-01 00:00:00
abstract::It was shown previously that the disruption of the ahrC gene encoding a predicted ArgR family transcription factor results in a severe defect in biofilm formation in vitro, as well as a significant attenuation of virulence of Enterococcus faecalis strain OG1RF in multiple experimental infection models. Using transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00269-18
更新日期:2018-08-24 00:00:00
abstract::Nishida, S. (Kanazawa University, Kanazawa, Japan), and G. Nakagawara. Isolation of toxigenic strains of Clostridium novyi from soil. J. Bacteriol. 88:1636-1640. 1964.-The cultural conditions for toxin production by Clostridium novyi were investigated, and the optimal constituents of a proper medium were determined. A...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.6.1636-1640.1964
更新日期:1964-12-01 00:00:00
abstract::Transferable plasmids play an important role in the dissemination of clindamycin-erythromycin resistance in Bacteroides fragilis. We previously described the isolation and properties of pBFTM10, a 14.9-kb ClnR transfer factor from B. fragilis TMP10. We also reported the isolation of a transfer-deficient deletion deriv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.23.7471-7480.1991
更新日期:1991-12-01 00:00:00
abstract::The antibacterial protein toxin colicin V is secreted from Escherichia coli cells by a dedicated export system that is a member of the multicomponent ATP-binding cassette (ABC) transporter family. At least three proteins, CvaA, CvaB, and TolC, are required for secretion via this signal sequence-independent pathway. In...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.21.6153-6159.1995
更新日期:1995-11-01 00:00:00
abstract::Rough variants of serovars from the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex were isolated in high frequency from pellicle growth of the wild-type strains. Rough morphology could be correlated with the lack of an outer cell wall sheath and its constituent C-mycoside glycopept...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.381-384.1982
更新日期:1982-04-01 00:00:00
abstract::Glucose-6-phosphate dehydrogenase was partially purified from both glucose-grown and iron-glucose-grown Thiobacillus ferrooxidans. The enzyme possesses a dual nucleotide specificity for either nicotinamide adenine dinucleotide phosphate (NADP) or nicotinamide adenine dinucleotide (NAD) and has a molecular weight of 11...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.343-352.1971
更新日期:1971-10-01 00:00:00
abstract::The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all prepar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.3.980-982.1968
更新日期:1968-03-01 00:00:00
abstract::Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.159-168.1983
更新日期:1983-07-01 00:00:00
abstract::Deoxyribonucleic acid (DNA), pulse labeled after ultraviolet irradiation of excision-defective mutants of Haemophilus influenzae, is of lower single strand molecular weight than that of unirradiated cells but approaches the size of DNA from unirradiated cells upon further incubation in growth medium. This gap-filling ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.176-181.1975
更新日期:1975-10-01 00:00:00
abstract::The Escherichia coli mutant Y16, which shows thermosensitive colony formation and filamentation with reduced amounts of penicillin-binding protein 3 (PBP3), has mutations in the ftsI gene encoding PBP3 and in the ftsH gene. The ftsI mutation markedly reduces the amount of PBP3 at 42 degrees C, whereas the amount of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.7.2416-2417.1992
更新日期:1992-04-01 00:00:00
abstract::The temperature-sensitive cyr1-2 mutant in Saccharomyces cerevisiae produces low levels of adenylate cyclase and cyclic AMP at 25 degrees C and is unable to synthesize repressible acid phosphatase at 25 degrees C. Suppressor mutants of cyr1-2 were isolated by detecting acid phosphatase activity. One of the dominant su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.7.2406-2408.1991
更新日期:1991-04-01 00:00:00
abstract::The direction of chromosome replication in a temperature-sensitive initiation mutant of Escherichia coli (CT28) is shown autoradiographically to be bidirectional. This mode of replication persists even when the rate of replication is reduced by slow growth in succinate minimal medium or in the presence of chlorampheni...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.346-352.1976
更新日期:1976-01-01 00:00:00
abstract::Onoue, Kaoru, (Kyushu University, Fukuoka, Japan), Masayasu Kitagawa, and Yuichi Yamamura. Chemical studies on cellular components of Bordetella pertussis. I. Purification and properties of agglutinogen. J. Bacteriol. 82:648-656. 1961.-A method is described for the preparation of the agglutinogen of Hemophilus pertuss...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.5.648-656.1961
更新日期:1961-11-01 00:00:00
abstract::Previously we have shown that flagella and the O-specific polysaccharide of lipopolysaccharide play a role in colonization of the potato root by plant growth-promoting Pseudomonas strains WCS374 and WCS358. In this paper, we describe a novel cell surface-exposed structure in Pseudomonas putida WCS358 examined with a s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.1955-1961.1996
更新日期:1996-04-01 00:00:00
abstract::Glycerol trinitrate (GTN) reductase, which enables Agrobacterium radiobacter to utilize GTN and related explosives as sources of nitrogen for growth, was purified and characterized, and its gene was cloned and sequenced. The enzyme was a 39-kDa monomeric protein which catalyzed the NADH-dependent reductive scission of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.24.7796-7802.1997
更新日期:1997-12-01 00:00:00
abstract::We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All strains tested could transport both of these opines, regardless of the presence or type of Ti plasmid (octopine or nopalin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.903-905.1991
更新日期:1991-01-01 00:00:00
abstract::Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.913-917.1971
更新日期:1971-03-01 00:00:00
abstract::One of the phosphatidyl glycerophosphate phosphatase genes of Escherichia coli, pgpA, was cloned, and its DNA sequence was determined. Its 507-base-pair open reading frame was consistent with the 18,000-molecular-weight product identified by a maxicell experiment. Between its possible promoter and methionine initiatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5110-5116.1988
更新日期:1988-11-01 00:00:00
abstract::Stocks, Peter K. (Louisiana State University, Baton Rouge), and C. S. McCleskey. Identity of the pink-pigmented methanol-oxidizing bacteria as Vibrio extorquens. J. Bacteriol. 88:1065-1070. 1964.-Pink-pigmented bacteria isolated from enrichment cultures of methane oxidizers were found to possess similar morphological,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.4.1065-1070.1964
更新日期:1964-10-01 00:00:00