Identification of the enzymatic basis for delta-aminolevulinic acid auxotrophy in a hemA mutant of Escherichia coli.

abstract：:The regulon of the sigma factor RpoS was defined in Geobacter sulfurreducens by using a combination of DNA microarray expression profiles and proteomics. An rpoS mutant was examined under steady-state conditions with acetate as an electron donor and fumarate as an electron acceptor and with additional transcriptional ...

journal_title：Journal of bacteriology

authors： Núñez C,Esteve-Núñez A,Giometti C,Tollaksen S,Khare T,Lin W,Lovley DR,Methé BA

更新日期：2006-04-01 00:00:00

abstract：:Membrane vesicles isolated from cells of bacillus subtilis W23 accumulate manganese in the presence of an energy source. The artificial electron donor system ascorbate and phenazine methosulfate or reduced nicotinamide adenine dinucleotide and phenazine methosulfate can supply the energy for the uptake. D-Lactate in t...

journal_title：Journal of bacteriology

authors： Bhattacharyya P

更新日期：1975-07-01 00:00:00

abstract：:The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a ...

journal_title：Journal of bacteriology

authors： Pradel E,Marck C,Boquet PL

更新日期：1990-02-01 00:00:00

abstract：:Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C. jejuni-mediated enteric disease is unclear; however, epitopes associated with the LOS are linked to the development of neurologic...

journal_title：Journal of bacteriology

authors： Oldfield NJ,Moran AP,Millar LA,Prendergast MM,Ketley JM

更新日期：2002-04-01 00:00:00

abstract：:Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...

journal_title：Journal of bacteriology

authors： Kreuzer KN,Cozzarelli NR

更新日期：1979-11-01 00:00:00

abstract：:The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...

journal_title：Journal of bacteriology

authors： Padhy RN,Hottat FG,Coene MM,Hoet PP

更新日期：1988-04-01 00:00:00

abstract：:Benzoate catabolism is thought to play a key role in aerobic bacterial degradation of biphenyl and polychlorinated biphenyls (PCBs). Benzoate catabolic genes were cloned from a PCB degrader, Rhodococcus sp. strain RHA1, by using PCR amplification and temporal temperature gradient electrophoresis separation. A nucleoti...

journal_title：Journal of bacteriology

authors： Kitagawa W,Miyauchi K,Masai E,Fukuda M

更新日期：2001-11-01 00:00:00

abstract：:Vibrio cholerae, the causative agent of the human diarrheal disease cholera, is a motile bacterium with a single polar flagellum. Motility has been implicated as a virulence determinant in some animal models of cholera, but the relationship between motility and virulence has not yet been clearly defined. We have begun...

journal_title：Journal of bacteriology

authors： Klose KE,Mekalanos JJ

更新日期：1998-01-01 00:00:00

abstract：:It previously has been proposed that the Escherichia coli SecB protein promotes the export of the maltose-binding protein (MBP) from the cytoplasm by preventing the folding of the precursor MBP (preMBP) into a translocation-incompetent conformation. The export of wild-type MBP is only partially blocked in SecB- cells....

journal_title：Journal of bacteriology

authors： Collier DN,Bassford PJ Jr

更新日期：1989-09-01 00:00:00

abstract：:Shigella spp. are the major cause of bacillary dysentery worldwide. The pathogenic process involves bacterial invasion and lysis of the phagocytic vacuole, followed by replication and movement within the cell cytoplasm and, ultimately, spread directly into adjacent cells. This study demonstrates that S. flexneri cytoc...

journal_title：Journal of bacteriology

authors： Way SS,Sallustio S,Magliozzo RS,Goldberg MB

更新日期：1999-02-01 00:00:00

abstract：:The regulatory properties of acetohydroxy acid synthetase (AHAS), the first enzyme in the biosynthetic pathway to valine and the second in the isoleucine pathway, were investigated in the fission yeast Schizosaccharomyces pombe. The enzyme was partially purified from crude extracts by protamine sulfate treatment, ammo...

journal_title：Journal of bacteriology

authors： McDonald RA,Satyanarayana T,Kaplan JG

更新日期：1973-04-01 00:00:00

abstract：:Gene conversion, defined as the nonreciprocal transfer of DNA, is one result of homologous recombination. Three steps in recombination could give rise to gene conversion: (i) DNA synthesis for repair of the degraded segment, (ii) Holliday junction migration, leading to heteroduplex formation, and (iii) repair of misma...

journal_title：Journal of bacteriology

authors： Castellanos M,Romero D

更新日期：2009-08-01 00:00:00

abstract：:Two riboflavin synthase activities (heavy and light) have been observed in earlier studies with Bacillus subtilis. The heavy enzyme is a complex of one molecule of light enzyme (consisting of three alpha subunits) and approximately 60 beta subunits (A. Bacher, R. Bauer, U. Eggers, H. Harders, and H. Schnepple, p. 729-...

journal_title：Journal of bacteriology

authors： Bacher A,Mailänder B

更新日期：1978-05-01 00:00:00

abstract：:All Escherichia coli strains so far examined possess a chromosomally encoded nanATEK-yhcH operon for the catabolism of sialic acids. These unique nine-carbon sugars are synthesized primarily by higher eukaryotes and can be used as carbon, nitrogen, and energy sources by a variety of microbial pathogens or commensals. ...

journal_title：Journal of bacteriology

authors： Kalivoda KA,Steenbergen SM,Vimr ER,Plumbridge J

更新日期：2003-08-01 00:00:00

abstract：:When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of isogenic mutants, with a beta-galactosidase gene transcriptionally fused to the inactivated target gene, was used to ide...

journal_title：Journal of bacteriology

authors： Prágai Z,Eschevins C,Bron S,Harwood CR

更新日期：2001-04-01 00:00:00

abstract：:Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cyste...

journal_title：Journal of bacteriology

authors： Mansilla MC,de Mendoza D

更新日期：1997-02-01 00:00:00

abstract：:Most bacteria are likely to face osmotic challenges, but there is yet much to learn about how such environmental changes affect the architecture of bacterial cells. Here, we report a cell-biological study in model organisms of the genus Streptomyces, which are actinobacteria that grow in a highly polarized fashion to ...

journal_title：Journal of bacteriology

authors： Fuchino K,Flärdh K,Dyson P,Ausmees N

更新日期：2016-12-13 00:00:00

abstract：:The bacterial response to stress is controlled by two proteins, RelA and SpoT. RelA generates the alarmone (p)ppGpp under amino acid starvation, whereas SpoT is responsible for (p)ppGpp hydrolysis and for synthesis of (p)ppGpp under a variety of cellular stress conditions. It is widely accepted that RelA is associated...

journal_title：Journal of bacteriology

authors： Jiang M,Sullivan SM,Wout PK,Maddock JR

更新日期：2007-09-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic bacterial pathogen which poses a major threat to long-term-hospitalized patients and individuals with cystic fibrosis. The capacity of P. aeruginosa to form biofilms is an important requirement for chronic colonization of human tissues and for persistence in implanted medical...

journal_title：Journal of bacteriology

authors： Vallet I,Diggle SP,Stacey RE,Cámara M,Ventre I,Lory S,Lazdunski A,Williams P,Filloux A

更新日期：2004-05-01 00:00:00

abstract：:The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined. The pspF gene encodes a sigma54 transcriptional activator of the phage shock protein (psp) operon (pspA to pspE). Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL and goaG, convergently oriente...

journal_title：Journal of bacteriology

authors： Jovanovic G,Model P

更新日期：1997-05-01 00:00:00

abstract：:The McrB restriction system of Escherichia coli K-12 is responsible for the biological inactivation of foreign DNA that contains 5-methylcytosine residues (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986). Within the McrB region of the chromosome is the mcrB gene, which encodes a protein of ...

journal_title：Journal of bacteriology

authors： Ross TK,Achberger EC,Braymer HD

更新日期：1989-04-01 00:00:00

abstract：:The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique protein...

journal_title：Journal of bacteriology

authors： Chromy BA,Choi MW,Murphy GA,Gonzales AD,Corzett CH,Chang BC,Fitch JP,McCutchen-Maloney SL

更新日期：2005-12-01 00:00:00

abstract：:Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its ac...

journal_title：Journal of bacteriology

authors： Ramírez S,Moreno R,Zafra O,Castán P,Vallés C,Berenguer J

更新日期：2000-04-01 00:00:00

abstract：:Citrate metabolism in Leuconostoc mesenteroides subspecies mesenteroides is associated with the generation of a proton motive force by a secondary mechanism (C. Marty-Teysset, C. Posthuma, J. S. Lolkema, P. Schmitt, C. Divies, and W. N. Konings, J. Bacteriol. 178:2178-2185, 1996). The pathway consists of four steps: (...

journal_title：Journal of bacteriology

authors： Marty-Teysset C,Lolkema JS,Schmitt P,Diviès C,Konings WN

更新日期：1996-11-01 00:00:00

abstract：:A group of enzymes were prepared from the culture fluids of streptococci belonging to groups A, B, C, G, and L, and from a strain of Streptococcus sanguis. These streptococcal enzymes (designated St-sialidases) released a substance shown to belong to the sialic acid group from the specific substrate BSM-St, a sialomuc...

journal_title：Journal of bacteriology

authors： Hayano S,Tanaka A

更新日期：1969-03-01 00:00:00

abstract：:In this study, we report the DNA sequence and biological analysis of a mycobacterial mercury resistance operon encoding a novel Hg(2+) transporter. MerH was found to transport mercuric ions in Escherichia coli via a pair of essential cysteine residues but only when coexpressed with the mercuric reductase. ...

journal_title：Journal of bacteriology

authors： Schué M,Dover LG,Besra GS,Parkhill J,Brown NL

更新日期：2009-01-01 00:00:00

abstract：:Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promote...

journal_title：Journal of bacteriology

authors： Qi Y,Kobayashi Y,Hulett FM

更新日期：1997-04-01 00:00:00

abstract：:The spoVM gene encodes a 26-amino-acid polypeptide that is essential for spore formation in Bacillus subtilis. A transposon insertion within the spoVM open reading frame has been shown to encode a chimeric protein which is biologically inactive and produces a phenotype identical to that of a deletion and insertion mut...

journal_title：Journal of bacteriology

authors： Cutting S,Anderson M,Lysenko E,Page A,Tomoyasu T,Tatematsu K,Tatsuta T,Kroos L,Ogura T

更新日期：1997-09-01 00:00:00

abstract：:The presence of the membrane lipid phosphatidylcholine (PC) in the bacterial membrane is critically important for many host-microbe interactions. The phospholipid N-methyltransferase PmtA from the plant pathogen Agrobacterium tumefaciens catalyzes the formation of PC by a three-step methylation of phosphatidylethanola...

journal_title：Journal of bacteriology

authors： Aktas M,Gleichenhagen J,Stoll R,Narberhaus F

更新日期：2011-07-01 00:00:00

abstract：:Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...

journal_title：Journal of bacteriology

authors： Pi J,Wookey PJ,Pittard AJ

更新日期：1993-11-01 00:00:00