S-adenosylmethionine-binding properties of a bacterial phospholipid N-methyltransferase.

Abstract:

:The presence of the membrane lipid phosphatidylcholine (PC) in the bacterial membrane is critically important for many host-microbe interactions. The phospholipid N-methyltransferase PmtA from the plant pathogen Agrobacterium tumefaciens catalyzes the formation of PC by a three-step methylation of phosphatidylethanolamine via monomethylphosphatidylethanolamine and dimethylphosphatidylethanolamine. The methyl group is provided by S-adenosylmethionine (SAM), which is converted to S-adenosylhomocysteine (SAH) during transmethylation. Despite the biological importance of bacterial phospholipid N-methyltransferases, little is known about amino acids critical for binding to SAM or phospholipids and catalysis. Alanine substitutions in the predicted SAM-binding residues E58, G60, G62, and E84 in A. tumefaciens PmtA dramatically reduced SAM-binding and enzyme activity. Homology modeling of PmtA satisfactorily explained the mutational results. The enzyme is predicted to exhibit a consensus topology of the SAM-binding fold consistent with cofactor interaction as seen with most structurally characterized SAM-methyltransferases. Nuclear magnetic resonance (NMR) titration experiments and (14)C-SAM-binding studies revealed binding constants for SAM and SAH in the low micromolar range. Our study provides first insights into structural features and SAM binding of a bacterial phospholipid N-methyltransferase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Aktas M,Gleichenhagen J,Stoll R,Narberhaus F

doi

10.1128/JB.01539-10

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

3473-81

issue

14

eissn

0021-9193

issn

1098-5530

pii

JB.01539-10

journal_volume

193

pub_type

杂志文章
  • Effect of oxygen on liquid holding recovery of Bacteroides fragilis.

    abstract::Liquid holding recovery (LHR) in ultraviolet-irradiated Bacteroides fragilis cells occurred under aerobic conditions but was inhibited by anaerobic conditions. The increase in survival after aerobic LHR resulted in an increase in the shoulder regions of the ultraviolet survival curves. Maximum LHR was obtained after h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.1-7.1981

    authors: Jones DT,Woods DR

    更新日期:1981-01-01 00:00:00

  • Definition of a minimal plasmid stabilization system from the broad-host-range plasmid RK2.

    abstract::The stable inheritance of the broad-host-range plasmid RK2 is due at least in part to functions within a region located at coordinates 32.8 to 35.9 kb, termed the RK2 par locus. This locus encodes four previously identified genes in two operons (parCBA and parD; M. Gerlitz, O. Hrabak, and H. Schwab, J. Bacteriol. 172:...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.24.8119-8132.1992

    authors: Roberts RC,Helinski DR

    更新日期:1992-12-01 00:00:00

  • Molecular cloning and functional expression of alternative oxidase from Candida albicans.

    abstract::The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.13.4098-4102.1999

    authors: Huh WK,Kang SO

    更新日期:1999-07-01 00:00:00

  • Transcriptional phase variation of a type III restriction-modification system in Helicobacter pylori.

    abstract::Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.23.6615-6624.2002

    authors: de Vries N,Duinsbergen D,Kuipers EJ,Pot RG,Wiesenekker P,Penn CW,van Vliet AH,Vandenbroucke-Grauls CM,Kusters JG

    更新日期:2002-12-01 00:00:00

  • Streptococcus faecium ATCC 9790 penicillin-binding proteins and penicillin sensitivity are heavily influenced by growth conditions: proposal for an indirect mechanism of growth inhibition by beta-lactams.

    abstract::The effects of variations in growth conditions on the penicillin response of Streptococcus faecium ATCC 9790 were studied. Changes in the growth temperature and medium composition were found to cause striking changes in the bacterial generation time, cellular penicillin sensitivity (minimum inhibitory concentration), ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.2.916-923.1983

    authors: Fontana R,Canepari P,Satta G,Coyette J

    更新日期:1983-05-01 00:00:00

  • Galactose transport in Saccharomyces cerevisiae. I. Nonmetabolized sugars as substrates and inducers of the galactose transport system.

    abstract::The inducible galactose transport system in bakers' yeast carries out the facilitated diffusion of the nonmetabolized galactose analogues d-fucose and l-arabinose. This capacity depends on the activity of the Ga 2 gene. In some strains, d-fucose and l-arabinose are also gratuitous inducers. Mutants in which the induci...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1727-1731.1968

    authors: Cirillo VP

    更新日期:1968-05-01 00:00:00

  • Composition of the fractions separated by polyacrylamide gel electrophoresis of the lipopolysaccharide of a marine bacterium.

    abstract::The sugar composition of lipopolysaccharide (LPS) isolated from whole cells of Alteromonas haloplanktis 214 (previously referred to as marine pseudomonas B-16, ATCC 19855), variant 3, of the lipid A, core, and side-chain fractions derived from it, and of the LPS fractions (LPS I, II, and III) obtained by subjecting it...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.158-167.1978

    authors: DiRienzo JM,MacLeod RA

    更新日期:1978-10-01 00:00:00

  • Prolonged stationary-phase incubation selects for lrp mutations in Escherichia coli K-12.

    abstract::Evolution by natural selection occurs in cultures of Escherichia coli maintained under carbon starvation stress. Mutants of increased fitness express a growth advantage in stationary phase (GASP) phenotype, enabling them to grow and displace the parent as the majority population. The first GASP mutation was identified...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.15.4361-4365.2000

    authors: Zinser ER,Kolter R

    更新日期:2000-08-01 00:00:00

  • Pediococcus cerevisiae mutant with altered transport of folates.

    abstract::A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.2.400-406.1975

    authors: Mandelbaum-Shavit F,Grossowicz N

    更新日期:1975-08-01 00:00:00

  • Genome sequence of Reyranella massiliensis, a bacterium associated with amoebae.

    abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01260-12

    authors: Pagnier I,Croce O,Robert C,Raoult D,La Scola B

    更新日期:2012-10-01 00:00:00

  • Evidence for extrusion of unfolded extracellular enzyme polypeptide chains through membranes of Bacillus amyloliquefaciens.

    abstract::The production of extracellular alpha-amylase and protease by protoplasts of Bacillus amyloliquefaciens has been achieved. The production of enzymically active protease was totally dependent on a high concentration of either Mg2+, Ca2+, or spermidine, but production of active alpha-amylase was not. This cation depende...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.806-814.1975

    authors: Sanders RL,May BK

    更新日期:1975-09-01 00:00:00

  • Coexpression of Escherichia coli obgE, Encoding the Evolutionarily Conserved Obg GTPase, with Ribosomal Proteins L21 and L27.

    abstract:UNLABELLED:Multiple essential small GTPases are involved in the assembly of the ribosome or in the control of its activity. Among them, ObgE (CgtA) has been shown recently to act as a ribosome antiassociation factor that binds to ppGpp, a regulator whose best-known target is RNA polymerase. The present study was aimed ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00159-16

    authors: Maouche R,Burgos HL,My L,Viala JP,Gourse RL,Bouveret E

    更新日期:2016-06-13 00:00:00

  • Membrane lipoteichoic acid is not a precursor to wall teichoic acid in pneumococci.

    abstract::The distribution of a pulse of teichoic acid-specific radiolabel between wall and membrane teichoic acids in pneumococci was constant over a subsequent chase period, suggesting that wall and membrane teichoic acids are biosynthesized simultaneously and independently. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.1.335-337.1975

    authors: Briles EB,Tomasz A

    更新日期:1975-04-01 00:00:00

  • Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters.

    abstract::We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3496-3499.1990

    authors: Farinha MA,Kropinski AM

    更新日期:1990-06-01 00:00:00

  • Interaction of the Gifsy-1 Xis protein with the Gifsy-1 attP sequence.

    abstract::The Gifsy-1 phage integrates site specifically into the Salmonella chromosome via an integrase-mediated site-specific recombination mechanism. Initial genetic analysis suggests that Gifsy-1 integrase-mediated excision of the Gifsy-1 phage is influenced by proteins encoded by both the Gifsy-1 and the Gifsy-2 phages. Ou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00577-07

    authors: Flanigan A,Gardner JF

    更新日期:2007-09-01 00:00:00

  • Purification of flagellar cores of Vibrio cholerae.

    abstract::A procedure is described for the purification of the cores of flagella sheared from Vibrio cholerae. V. cholerae is a monotrichous organism whose flagellar core (FC) is enclosed within a sheath. The purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.2.1121-1128.1977

    authors: Yang GC,Schrank GD,Freeman BA

    更新日期:1977-02-01 00:00:00

  • Formation of an Intramolecular Periplasmic Disulfide Bond in TcpP Protects TcpP and TcpH from Degradation in Vibrio cholerae.

    abstract:UNLABELLED:TcpP and ToxR coordinately regulate transcription of toxT, the master regulator of numerous virulence factors in Vibrio cholerae. TcpP and ToxR are membrane-localized transcription factors, each with a periplasmic domain containing two cysteines. In ToxR, these cysteines form an intramolecular disulfide bond...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00338-15

    authors: Morgan SJ,French EL,Thomson JJ,Seaborn CP,Shively CA,Krukonis ES

    更新日期:2015-11-16 00:00:00

  • Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron.

    abstract:UNLABELLED:The role of chromosomal toxin-antitoxin (TA) systems, which are ubiquitous within the genomes of free-living bacteria, is still debated. We have scanned the Vibrio cholerae N16961 genome for class 2 TA genes and identified 18 gene pair candidates. Interestingly, all but one are located in the chromosome 2 su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00108-15

    authors: Iqbal N,Guérout AM,Krin E,Le Roux F,Mazel D

    更新日期:2015-07-01 00:00:00

  • Reproduction and morphogenesis of Rickettsiella chironomi, an unusual intracellular procaryotic parasite of midge larvae.

    abstract::The reproduction and morphogenesis of Rickettsiella chironomi, an unusual procaryotic parasite of midge larvae, was studied with electron microscopy. The morphogenic cycle was similar to that found in chlamydia and consisted of four major cell types: (i) medium-sized spherical initial bodies (ca. 1 micrometers in diam...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.2.995-1002.1980

    authors: Federici BA

    更新日期:1980-08-01 00:00:00

  • Expression of the iron-activated nspA and secY genes in Neisseria meningitidis group B by Fur-dependent and -independent mechanisms.

    abstract::Our whole-genome microarray studies of Neisseria meningitidis MC58 previously identified a set of 153 genes whose transcription was activated during growth in iron. In this study, Fur-mediated regulation of the iron-activated nspA gene was confirmed, whereas iron-activated regulation of the secY gene was demonstrated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01638-06

    authors: Shaik YB,Grogan S,Davey M,Sebastian S,Goswami S,Szmigielski B,Genco CA

    更新日期:2007-01-01 00:00:00

  • Expression of a gene in a 400-base-pair fragment of colicin plasmid ColE2-P9 is sufficient to cause host cell lysis.

    abstract::The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (molecular weight, 9,00...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.1.109-114.1983

    authors: Pugsley AP,Schwartz M

    更新日期:1983-10-01 00:00:00

  • Intracellular concentrations of 65 species of transcription factors with known regulatory functions in Escherichia coli.

    abstract::The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01579-14

    authors: Ishihama A,Kori A,Koshio E,Yamada K,Maeda H,Shimada T,Makinoshima H,Iwata A,Fujita N

    更新日期:2014-08-01 00:00:00

  • Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among.

    abstract::Weiss, Emilio (Naval Medical Research Institute, Bethesda, Md.) and Harry R. Dressler. Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among strains. J. Bacteriol. 83:409-414. 1962.-A further effort to produce genetically labeled Rickettsia pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.409-414.1962

    authors: WEISS E,DRESSLER HR

    更新日期:1962-02-01 00:00:00

  • Characterization of an ADP-ribosyltransferase toxin (AexT) from Aeromonas salmonicida subsp. salmonicida.

    abstract::An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.7.1851-1858.2002

    authors: Braun M,Stuber K,Schlatter Y,Wahli T,Kuhnert P,Frey J

    更新日期:2002-04-01 00:00:00

  • Biochemical characterization of UDP-Gal:GlcNAc-pyrophosphate-lipid β-1,4-Galactosyltransferase WfeD, a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating-unit synthesis.

    abstract::The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00737-10

    authors: Xu C,Liu B,Hu B,Han Y,Feng L,Allingham JS,Szarek WA,Wang L,Brockhausen I

    更新日期:2011-01-01 00:00:00

  • Annotated genome sequence of Lactobacillus pentosus MP-10, which has probiotic potential, from naturally fermented Aloreña green table olives.

    abstract::Lactobacillus pentosus MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of L...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05171-11

    authors: Abriouel H,Benomar N,Pulido RP,Cañamero MM,Gálvez A

    更新日期:2011-09-01 00:00:00

  • Composition of cardiolipin molecular species in Escherichia coli.

    abstract::The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1047-1051.1980

    authors: Yokota K,Kanamoto R,Kito M

    更新日期:1980-03-01 00:00:00

  • Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins.

    abstract::Type VI secretion systems (T6SS) are bacteriophage-derived macromolecular machines responsible for the release of at least two proteins in the milieu, which are thought to form an extracellular appendage. Although several T6SS have been shown to be involved in the virulence of animal and plant pathogens, clusters enco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00029-11

    authors: Bernard CS,Brunet YR,Gavioli M,Lloubès R,Cascales E

    更新日期:2011-05-01 00:00:00

  • Genetics of xanthan production in Xanthomonas campestris: the xanA and xanB genes are involved in UDP-glucose and GDP-mannose biosynthesis.

    abstract::The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectiv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.1.191-199.1992

    authors: Köplin R,Arnold W,Hötte B,Simon R,Wang G,Pühler A

    更新日期:1992-01-01 00:00:00

  • The gene yjcG, cotranscribed with the gene acs, encodes an acetate permease in Escherichia coli.

    abstract::We isolated an Escherichia coli mutant strain that suppresses the glycolate-negative phenotype of a strain deficient in both GlcA and LldP transporters of this compound. This suppressing phenotype was assigned to yjcG, a gene whose function was previously unknown, which was found to encode a membrane protein able to t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.21.6448-6455.2003

    authors: Gimenez R,Nuñez MF,Badia J,Aguilar J,Baldoma L

    更新日期:2003-11-01 00:00:00