Abstract:
:An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ExoT exotoxins of Pseudomonas aeruginosa and to the YopE cytotoxin of different Yersinia species. The aexT gene was detected in all of the 12 A. salmonicida subsp. salmonicida strains tested but was absent from all other Aeromonas species. Recombinant AexT produced in Escherichia coli possesses enzymatic ADP-ribosyltransferase activity. Monospecific polyclonal antibodies directed against purified recombinant AexT detected the toxin produced by A. salmonicida subsp. salmonicida and cross-reacted with ExoS and ExoT of P. aeruginosa. AexT toxin could be detected in a wild type (wt) strain of A. salmonicida subsp. salmonicida freshly isolated from a fish with furunculosis; however, its expression required contact with RTG-2 rainbow trout gonad cells. Under these conditions, the AexT protein was found to be intracellular or tightly cell associated. No AexT was found when A. salmonicida subsp. salmonicida was incubated in cell culture medium in the absence of RTG-2 cells. Upon infection with wt A. salmonicida subsp. salmonicida, the fish gonad RTG-2 cells rapidly underwent significant morphological changes. These changes were demonstrated to constitute cell rounding, which accompanied induction of production of AexT and which led to cell lysis after extended incubation. An aexT mutant which was constructed from the wt strain with an insertionally inactivated aexT gene by allelic exchange had no toxic effect on RTG-2 cells and was devoid of AexT production. Hence AexT is directly involved in the toxicity of A. salmonicida subsp. salmonicida for RTG-2 fish cells.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Braun M,Stuber K,Schlatter Y,Wahli T,Kuhnert P,Frey Jdoi
10.1128/jb.184.7.1851-1858.2002keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
1851-8issue
7eissn
0021-9193issn
1098-5530journal_volume
184pub_type
杂志文章abstract::In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.413-420.1972
更新日期:1972-10-01 00:00:00
abstract::The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.5.1539-1541.1994
更新日期:1994-03-01 00:00:00
abstract::Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00715-08
更新日期:2008-12-01 00:00:00
abstract::cat-86 is a promoter-deficient plasmid gene that encodes chloramphenicol acetyltransferase (CAT). Insertion of a promoter at a site 144 base pairs 5' to the cat-86 coding sequence activates transcription of the gene and allows cat-86 to specify chloramphenicol-inducible CAT activity in Bacillus subtilis. Induction of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.967-972.1987
更新日期:1987-03-01 00:00:00
abstract::In some media, Bacillus subtilis can maintain a prolonged stationary growth phase; however, in other media, nutrient depletion triggers a complex differentiation that culminates in production of a dormant endospore. This differentiation requires the expression of many genes. We found that during the stationary phase i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.709-715.1990
更新日期:1990-02-01 00:00:00
abstract::A fragment of the Lactococcus lactis chromosome containing an open reading frame of 265 codons, denoted bglR, has been characterized. The polypeptide encoded by bglR shares 36 to 30% sequence identity with a family of regulatory proteins including ArbG from Erwinia chrysanthemi, BglG from Escherichia coli, and SacT an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5681-5685.1994
更新日期:1994-09-01 00:00:00
abstract::A RecA-independent chromosomal rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes which affects slo expression was identified. PCR analysis was used to demonstrate that this kind of rearrangement was found in several strains of different lineages. Chromosomal loci involved ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.1857-1869.2003
更新日期:2003-03-01 00:00:00
abstract::The gene for staphylococcal enterotoxin type E (entE) was cloned from Staphylococcus aureus into plasmid vector pBR322 and introduced into Escherichia coli. A staphylococcal enterotoxin type E-producing E. coli strain was isolated. The complete nucleotide sequence of the cloned structural entE gene and the N-terminal ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.2954-2960.1988
更新日期:1988-07-01 00:00:00
abstract::The genes encoding the rrn operons, the 4.5S and 6S RNAs, elements of protein secretion, and outer membrane proteins F and I, and regulatory as well as structural genes for exotoxin A, alkaline phosphatase, and alginate and tryptophan biosynthesis, were assigned on the SpeI/DpnI macrorestriction map of the Pseudomonas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.327-330.1992
更新日期:1992-01-01 00:00:00
abstract::Adsorption of phages T4, T5, and BF23 to previously starved Escherichia coli cells triggered the immediate release of respiratory control. A similar stimulation of respiration was induced after T4 ghost attachment, showing that this process was independent of the mechanism of DNA injection. Rather, this change in the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.179-182.1985
更新日期:1985-01-01 00:00:00
abstract::The ribG gene at the 5' end of the riboflavin operon of Bacillus subtilis and a reading frame at 442 kb on the Escherichia coli chromosome (subsequently designated ribD) show similarity with deoxycytidylate deaminase and with the RIB7 gene of Saccharomyces cerevisiae. The ribG gene of B. subtilis and the ribD gene of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.6.2022-2028.1997
更新日期:1997-03-01 00:00:00
abstract::If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.435-444.1975
更新日期:1975-10-01 00:00:00
abstract::Methanogenesis from the non-physiological C1 donors thioproline, thiazolidine, hexamethylenetetramine, formaldehyde (HCHO), and HOCH2-S-coenzyme M (CoM) was catalyzed by cell extracts of Methanobacterium thermoautotrophicum under a hydrogen atmosphere. Tetrahydromethanopterin (H4MPT) and HS-CoM were required in the re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.2.696-701.1985
更新日期:1985-02-01 00:00:00
abstract::We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.1.111-118.2002
更新日期:2002-01-01 00:00:00
abstract::Many gram-negative bacteria harbor a copper/zinc-containing superoxide dismutase (CuZnSOD) in their periplasms. In pathogenic bacteria, one role of this enzyme may be to protect periplasmic biomolecules from superoxide that is released by host phagocytic cells. However, the enzyme is also present in many nonpathogens ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00554-06
更新日期:2006-09-01 00:00:00
abstract::The nucleotide sequence of tnaB of the tryptophanase operon of Escherichia coli is presented. TnaB is a tryptophan-specific permease that is homologous to Mtr, a second tryptophan-specific permease, and to TyrP, a tyrosine-specific permease. Each member of this family appears to contain 11 membrane-spanning domains. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.10.3231-3234.1991
更新日期:1991-05-01 00:00:00
abstract::Sinorhizobium meliloti is a free-living soil bacterium which is capable of establishing a symbiotic relationship with the alfalfa plant (Medicago sativa). This symbiosis involves a network of bacterium-host signaling, as well as the potential for bacterium-bacterium communication, such as quorum sensing. In this study...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.13.3466-3475.2002
更新日期:2002-07-01 00:00:00
abstract::A transformable strain of Bacillus subtilis 6160, a derivative of B. subtilis 168, produces three kinds of casein hydrolytic enzymes (alkaline protease, neutral protease, and esterase) in a culture medium. B. natto IAM 1212 produces 15 to 20 times as much total proteolytic activity as does B. subtilis. Extracellular p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.1.82-91.1974
更新日期:1974-07-01 00:00:00
abstract::Forty years ago, Coulter and Talalay (A. W. Coulter and P. Talalay, J. Biol. Chem. 243:3238-3247, 1968) established the oxygenase-dependent pathway for the degradation of testosterone by aerobes. The oxic testosterone catabolic pathway involves several oxygen-dependent reactions and is not available for anaerobes. Sin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00331-11
更新日期:2011-09-01 00:00:00
abstract::Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708 was located on a 6.5-kb EcoRI fragment of the chromosomal DNA. This fragment was cloned into bacteriophage lambda gt11, and a 2.9-kb piece of this ins...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.15.4558-4569.1991
更新日期:1991-08-01 00:00:00
abstract::A new mutation in Escherichia coli, designated gcvA1, that results in noninducible expression of both gcv and a gcvT-lacZ gene fusion was isolated. A plasmid carrying the wild-type gcvA gene complemented the mutation and restored glycine-inducible gcv and gcvT-lacZ gene expression. These results suggest that gcvA enco...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.3.902-904.1993
更新日期:1993-02-01 00:00:00
abstract::Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary phase, strain 749/C organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.1002-1009.1969
更新日期:1969-11-01 00:00:00
abstract::We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. In fact, the addi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.795-800.1986
更新日期:1986-06-01 00:00:00
abstract::An IMP dehydrogenase gene was isolated from Candida albicans on a approximately 2.9-kb XbaI genomic DNA fragment. The putative Candida IMP dehydrogenase gene (IMH3) encodes a protein of 521 amino acids with extensive sequence similarity to the IMP dehydrogenases of Saccharomyces cerevisiae and various other organisms....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.7.2331-2338.1997
更新日期:1997-04-01 00:00:00
abstract::Altenbern, Robert A. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.). Growth of L forms of streptomycin-dependent Proteus mirabilis. J. Bacteriol. 85:619-622. 1963.-A streptomycin-dependent mutant of Proteus mirabilis produces L forms on penicillin-containing medium when dihydrostreptomycin is also p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.3.619-622.1963
更新日期:1963-03-01 00:00:00
abstract::We have sequenced a DNA fragment containing the pneumococcal bacteriophage HB-3 hbl gene, which codes for the phage lytic amidase. A remarkable nucleotide similarity (87.1%) between the lytA gene, coding for the pneumococcal amidase, the major autolysin of Streptococcus pneumoniae, and the hbl gene was found. This sim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.5064-5070.1990
更新日期:1990-09-01 00:00:00
abstract::Despite the fact that phosphoenolpyruvate carboxylase (PEPC) activity has been measured and in some cases even purified from some Archaea, the gene responsible for this activity has not been elucidated. Using sensitive sequence comparison methods, we detected a highly conserved, uncharacterized archaeal gene family th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7754-7762.2004
更新日期:2004-11-01 00:00:00
abstract::Seventeen strains of mycoplasmata representing 11 named species were compared serologically by three parameters: growth inhibition on agar, double immunodiffusion, and complement fixation. In growth-inhibition studies, a strain labeled Mycoplasma histotropicum was found related to and perhaps best classified as M. pul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1044-1055.1969
更新日期:1969-06-01 00:00:00
abstract::The relationships of five feedback inhibitors for the Bacillus licheniformis glutamine synthetase were investigated. The inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. Mixtures of l-glutamine and adenosine-5'-monophosphate (AMP) or histidine and AMP c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.1016-1024.1967
更新日期:1967-10-01 00:00:00
abstract::Integrating conjugative elements (ICEs) of the SXT/R391 family are major contributors to the spread of antibiotic resistance genes. These elements also catalyze their own diversity by promoting inter-ICE recombination through the action of the RecA-independent homologous recombination system that they encode. Here, we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02090-12
更新日期:2013-05-01 00:00:00