The mRNA for an inducible chloramphenicol acetyltransferase gene is cleaved into discrete fragments in Bacillus subtilis.

Abstract:

:cat-86 is a promoter-deficient plasmid gene that encodes chloramphenicol acetyltransferase (CAT). Insertion of a promoter at a site 144 base pairs 5' to the cat-86 coding sequence activates transcription of the gene and allows cat-86 to specify chloramphenicol-inducible CAT activity in Bacillus subtilis. Induction of cat-86 by chloramphenicol has been shown to result from a regulatory event that activates translation of cat-86 mRNA that is present in cells before the addition of inducer (E. J. Duvall and P. S. Lovett, Proc. Natl. Acad. Sci. USA 83:3939-3943, 1986). In the present study we show an unusual property of cat-86 mRNA. Full-length cat-86 transcripts, consisting of 920 nucleotides (nt), are cleaved in B. subtilis to yield two predominant fragmentation products: an 810-nt species that lacks sequences present at the 5' end of the 920-nt species and a 720-nt species that lacks sequences present at the 3' end of the 920-nt species. A third fragmentation product consisting of 620 nt may result from the cleavage of a single 920-nt transcript at both the 5' and 3' ends. The sequences which are missing from the 720- and 620-nt species suggest that these transcripts cannot be translated into functional CAT. The 810-nt species lacks sequences from the 5' regulatory region, and it is not yet certain whether or not translation of this species can be induced by chloramphenicol. The ratio of 920-nt molecules/720-nt molecules in rifampin-treated cells is increased when the cells are grown in chloramphenicol. Therefore, induction may partially stabilize full-length cat-86 transcripts against inactivation by a novel processing-like system.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ambulos NP Jr,Duvall EJ,Lovett PS

doi

10.1128/jb.169.3.967-972.1987

subject

Has Abstract

pub_date

1987-03-01 00:00:00

pages

967-72

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

杂志文章
  • Nucleotide sequence of Escherichia coli pabB indicates a common evolutionary origin of p-aminobenzoate synthetase and anthranilate synthetase.

    abstract::Biochemical and immunological experiments have suggested that the Escherichia coli enzyme p-aminobenzoate synthetase and anthranilate synthetase are structurally related. Both enzymes are composed of two nonidentical subunits. Anthranilate synthetase is composed of proteins encoded by the genes trp(G)D and trpE, where...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.57-62.1984

    authors: Goncharoff P,Nichols BP

    更新日期:1984-07-01 00:00:00

  • DNA microarray analysis of genome dynamics in Yersinia pestis: insights into bacterial genome microevolution and niche adaptation.

    abstract::Genomics research provides an unprecedented opportunity for us to probe into the pathogenicity and evolution of the world's most deadly pathogenic bacterium, Yersinia pestis, in minute detail. In our present work, extensive microarray analysis in conjunction with PCR validation revealed that there are considerable gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.15.5138-5146.2004

    authors: Zhou D,Han Y,Song Y,Tong Z,Wang J,Guo Z,Pei D,Pang X,Zhai J,Li M,Cui B,Qi Z,Jin L,Dai R,Du Z,Bao J,Zhang X,Yu J,Wang J,Huang P,Yang R

    更新日期:2004-08-01 00:00:00

  • Metabolism of D-arabinose by Aerobacter aerogenes: purification of the isomerase.

    abstract::In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.293-299.1971

    authors: Oliver EJ,Mortlock RP

    更新日期:1971-10-01 00:00:00

  • Expression of glnA in the cyanobacterium Synechococcus sp. strain PCC 7942 is initiated from a single nif-like promoter under various nitrogen conditions.

    abstract::The glnA mRNA, encoding glutamine synthetase, is differentially accumulated in the cyanobacterium Synechococcus sp. strain PCC 7942 in media containing different nitrogen sources. With the different nitrogen compounds, transcription of glnA initiated at a single site located -146 nucleotides upstream of the translatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.23.7727-7731.1993

    authors: Cohen-Kupiec R,Gurevitz M,Zilberstein A

    更新日期:1993-12-01 00:00:00

  • Differential regulation of synthesis of multiple forms of specific endoglucanases by Trichoderma reesei QM9414.

    abstract::A method consisting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent detection of endoglucanases by blotting with a polyclonal antibody against endoglucanase I was used to investigate the effect of induction and carbon catabolite derepression on the synthesis of multiple forms of endoglucana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.8.3689-3693.1988

    authors: Messner R,Gruber F,Kubicek CP

    更新日期:1988-08-01 00:00:00

  • Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids.

    abstract::Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The op...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.153-160.1979

    authors: Emerich DW,Ruiz-Argüeso T,Ching TM,Evans HJ

    更新日期:1979-01-01 00:00:00

  • Integration of rotation and piston motions in coiled-coil signal transduction.

    abstract::A coordinated response to a complex and dynamic environment requires an organism to simultaneously monitor and interpret multiple signaling cues. In bacteria and some eukaryotes, environmental responses depend on the histidine autokinases (HKs). For example, VirA, a large integral membrane HK from Agrobacterium tumefa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00459-07

    authors: Gao R,Lynn DG

    更新日期:2007-08-01 00:00:00

  • Importance of anaerobic superoxide dismutase synthesis in facilitating outgrowth of Escherichia coli upon entry into an aerobic habitat.

    abstract::The manganese-containing isozyme of superoxide dismutase (MnSOD) is synthesized by Escherichia coli only during aerobiosis, in accordance with the fact that superoxide can be formed only in aerobic environments. In contrast, E. coli continues to synthesize the iron-containing isozyme (FeSOD) even in the absence of oxy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.24.7653-7658.1994

    authors: Kargalioglu Y,Imlay JA

    更新日期:1994-12-01 00:00:00

  • Structure of an acidic exopolysaccharide of Pseudomonas marginalis HT041B.

    abstract::The exopolysaccharide of Pseudomonas marginalis HT041B has been characterized as a 1,3-linked galactoglucan in which galactose and glucose are in the alpha- and beta-anomeric configurations, respectively. The polysaccharide is substituted with pyruvate at the 4 and 6 positions of galactose and with succinic acid at ei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.3.1760-1762.1989

    authors: Osman SF,Fett WF

    更新日期:1989-03-01 00:00:00

  • Regulation of Bacillus subtilis sigmaH (spo0H) and AbrB in response to changes in external pH.

    abstract::The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6778-6787.1997

    authors: Cosby WM,Zuber P

    更新日期:1997-11-01 00:00:00

  • Natural and immune human antibodies reactive with antigens of virulent Neisseria gonorrhoeae: immunoglobulins G, M, And A.

    abstract::Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for huma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.141-148.1967

    authors: Cohen IR

    更新日期:1967-07-01 00:00:00

  • Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.

    abstract::Bacillus subtilis contains three Fur homologs: Fur, PerR, and Zur. Despite significant sequence similarities, they respond to different stimuli and regulate different sets of genes. DNA target site comparisons indicate that all three paralogs recognize operators with a core 7-1-7 inverted repeat. The corresponding con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.21.6348-6357.2003

    authors: Fuangthong M,Helmann JD

    更新日期:2003-11-01 00:00:00

  • Isolation of Klebsiella aerogenes mutants cis-dominant for glutamine synthetase expression.

    abstract::We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glnA region and co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.671-679.1980

    authors: Rothstein DM,Magasanik B

    更新日期:1980-02-01 00:00:00

  • Electron microscopy of single-stranded structures in the DNA of competent Haemophilus influenzae cells.

    abstract::Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence developme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.565-571.1987

    authors: McCarthy D,Kupfer DM

    更新日期:1987-02-01 00:00:00

  • Characterization and functional analysis of atl, a novel gene encoding autolysin in Streptococcus suis.

    abstract::Streptococcus suis serotype 2 (S. suis 2) is an important swine and human pathogen responsible for septicemia and meningitis. A novel gene, designated atl and encoding a major autolysin of S. suis 2 virulent strain HA9801, was identified and characterized in this study. The Atl protein contains 1,025 amino acids with ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06231-11

    authors: Ju CX,Gu HW,Lu CP

    更新日期:2012-03-01 00:00:00

  • DE NOVO SYNTHESIS OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS.

    abstract::Welker, N. E. (Western Reserve University, Cleveland, Ohio and University of Illinois, Urbana), and L. Leon Campbell. De novo synthesis of alpha-amylase by Bacillus stearothermophilus. J. Bacteriol. 86:1202-1210. 1963.-The pH optimum for the synthesis of alpha-amylase by washed-cell suspensions was 6.7. alpha-Amylase ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.6.1202-1210.1963

    authors: WELKER NE,CAMPBELL LL

    更新日期:1963-12-01 00:00:00

  • Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica.

    abstract::A collection of 169 strains, including 91 obtained from cases of gastroenteritis and 41 from localized tissue infections and infections of the eye and ear, was submitted to an extensive nutritional, physiological, and morphological characterization. The nutritional and physiological data obtained from these strains, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.3.1144-1155.1973

    authors: Baumann P,Baumann L,Reichelt JL

    更新日期:1973-03-01 00:00:00

  • Symbiotic autoregulation of nifA expression in Rhizobium leguminosarum bv. viciae.

    abstract::NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae UPM791, the nifA gene is part of a gene cluster (orf71 orf79 fixW orf5 fixABCX nifAB) separated by 896 bp from an upstream and divergent truncated duplication of nifH (DeltanifH). Sy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.19.6586-6594.2004

    authors: Martínez M,Palacios JM,Imperial J,Ruiz-Argüeso T

    更新日期:2004-10-01 00:00:00

  • The Bacillus subtilis signaling protein SpoIVB defines a new family of serine peptidases.

    abstract::The protein SpoIVB plays a key role in signaling in the final sigma(K) checkpoint of Bacillus subtilis. This regulatory mechanism coordinates late gene expression during development in this organism and we have recently shown SpoIVB to be a serine peptidase. SpoIVB signals by transiting a membrane, undergoing self-cle...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.191-199.2002

    authors: Hoa NT,Brannigan JA,Cutting SM

    更新日期:2002-01-01 00:00:00

  • Comparison of the polypeptide composition of Escherichia coli outer membranes prepared by two methods.

    abstract::Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.425-427.1980

    authors: Chopra I,Shales SW

    更新日期:1980-10-01 00:00:00

  • Medium-dependent variation of deoxyribonucleic acid segregation in Escherichia coli.

    abstract::The degree to which deoxyribonucleic acid segregates nonrandomly has been investigated for Escherichia coli B/r growing in different media. The degree of nonrandom segregation observed is dependent on the medium, with segregation becoming less random as the growth rate decreases. This indicates that there must be some...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.118-127.1977

    authors: Cooper S,Weinberger M

    更新日期:1977-04-01 00:00:00

  • Effect of osmolarity on phagocytosis.

    abstract::Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.), William Shirley, and John S. Baumstark. Effect of osmolarity on phagocytosis. J. Bacteriol. 85:306-313. 1963.-The effect of a number of different compounds on phagocytosis was studied. Phagocytosis was monitored by morphological and biochemical means. It was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.2.306-313.1963

    authors: SBARRA AJ,SHIRLEY W,BAUMSTARK JS

    更新日期:1963-02-01 00:00:00

  • Loss of low-level antibiotic resistance in Neisseria gonorrhoeae due to env mutations.

    abstract::Mutations (env) which resulted in increased sensitivity of gonococci to diverse compounds were studied by transformation. Strains carrying an env mutation were more sensitive than wild-type strains to several antibiotics, dyes, and detergents. The env mutations resulted in complete phenotypic suppression of low-level ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.2.750-756.1975

    authors: Sarubbi FA Jr,Sparling PF,Blackman E,Lewis E

    更新日期:1975-11-01 00:00:00

  • Ferric reductase activity in Azotobacter vinelandii and its inhibition by Zn2+.

    abstract::Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.4031-4037.1989

    authors: Huyer M,Page WJ

    更新日期:1989-07-01 00:00:00

  • Time of enterotoxin formation and release during sporulation of Clostridium perfringens type A.

    abstract::Clostridium perfringens enterotoxin was detected intracellularly about 3 hr after the inoculation of vegetative cells into sporulation medium. The subsequent increase in intracellular enterotoxin concentration roughly paralleled but followed by 2.5 to 5 hr the increase in number of heat-resistant spores. The increase ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.932-936.1973

    authors: Duncan CL

    更新日期:1973-02-01 00:00:00

  • Host cell and ultraviolet reactivation of ultraviolet-irradiated Mycoplasmaviruses.

    abstract::The mycoplasma Acholeplasma laidlawii was shown to have mechanisms for both host cell and ultraviolet (UV) reactivation of UV-irradiated mycoplasmaviruses. Host cell reactivation was examined by comparing the survival abilities of UV-irradiated double-stranded deoxyribonucleic acid mycoplasmavirus plated on both untre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1424-1427.1977

    authors: Das J,Nowak JA,Maniloff J

    更新日期:1977-03-01 00:00:00

  • Novel missense mutations that affect the transport function of MalK, the ATP-binding-cassette subunit of the Salmonella enterica serovar typhimurium maltose transport system.

    abstract::We report on novel mutations in the malK gene of Salmonella enterica serovar Typhimurium, encoding the ATPase subunit of the maltose transporter (MalFGK(2)). Biochemical analysis suggests that (i) L86 might be involved in a signaling step during substrate translocation and (ii) E306 may be critical for the structural ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.5.1432-1436.2000

    authors: Hunke S,Landmesser H,Schneider E

    更新日期:2000-03-01 00:00:00

  • Upstream migration of Xylella fastidiosa via pilus-driven twitching motility.

    abstract::Xylella fastidiosa is a xylem-limited nonflagellated bacterium that causes economically important diseases of plants by developing biofilms that block xylem sap flow. How the bacterium is translocated downward in the host plant's vascular system against the direction of the transpiration stream has long been a puzzlin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.16.5560-5567.2005

    authors: Meng Y,Li Y,Galvani CD,Hao G,Turner JN,Burr TJ,Hoch HC

    更新日期:2005-08-01 00:00:00

  • EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY.

    abstract::LaBrec, Eugene H., Herman Schneider, Thomas J. Magnani, and Samuel B. Formal. Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. J. Bacteriol. 88:1503-1518. 1964.-A parent strain of Shigella flexneri 2a and a colonial mutant derived from it were studied in three animal models....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.5.1503-1518.1964

    authors: Labrec EH,Schneider H,Magnani TJ,Formal SB

    更新日期:1964-11-01 00:00:00

  • The C-terminus of substrates is critical but not sufficient for their degradation by the Pseudomonas aeruginosa CtpA protease.

    abstract::Bacterial carboxyl-terminal processing proteases (CTPs) are widely conserved and have been linked to important processes including signal transduction, cell wall metabolism, and virulence. However, the features that target proteins for CTP-dependent cleavage are unclear. Studies of the Escherichia coli CTP Prc suggest...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00174-20

    authors: Chung S,Darwin AJ

    更新日期:2020-06-01 00:00:00