The mRNA for an inducible chloramphenicol acetyltransferase gene is cleaved into discrete fragments in Bacillus subtilis.


:cat-86 is a promoter-deficient plasmid gene that encodes chloramphenicol acetyltransferase (CAT). Insertion of a promoter at a site 144 base pairs 5' to the cat-86 coding sequence activates transcription of the gene and allows cat-86 to specify chloramphenicol-inducible CAT activity in Bacillus subtilis. Induction of cat-86 by chloramphenicol has been shown to result from a regulatory event that activates translation of cat-86 mRNA that is present in cells before the addition of inducer (E. J. Duvall and P. S. Lovett, Proc. Natl. Acad. Sci. USA 83:3939-3943, 1986). In the present study we show an unusual property of cat-86 mRNA. Full-length cat-86 transcripts, consisting of 920 nucleotides (nt), are cleaved in B. subtilis to yield two predominant fragmentation products: an 810-nt species that lacks sequences present at the 5' end of the 920-nt species and a 720-nt species that lacks sequences present at the 3' end of the 920-nt species. A third fragmentation product consisting of 620 nt may result from the cleavage of a single 920-nt transcript at both the 5' and 3' ends. The sequences which are missing from the 720- and 620-nt species suggest that these transcripts cannot be translated into functional CAT. The 810-nt species lacks sequences from the 5' regulatory region, and it is not yet certain whether or not translation of this species can be induced by chloramphenicol. The ratio of 920-nt molecules/720-nt molecules in rifampin-treated cells is increased when the cells are grown in chloramphenicol. Therefore, induction may partially stabilize full-length cat-86 transcripts against inactivation by a novel processing-like system.


J Bacteriol


Journal of bacteriology


Ambulos NP Jr,Duvall EJ,Lovett PS




Has Abstract


1987-03-01 00:00:00












  • Comparison of the two isofunctional enol-lactone hydrolases from Acinetobacter calcoaceticus.

    abstract::The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Katagiri M,Wheelis ML

    更新日期:1971-05-01 00:00:00

  • Convenient transduction of recA with bacteriophage T4GT7.

    abstract::The generalized transducing phage T4GT7 grew well on recA strains of Escherichia coli and transduced recA into F- and Hfr strains of E. coli at high frequency. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Plakidou S,Moffat KG,Salmond GP,Mackinnon G

    更新日期:1984-09-01 00:00:00

  • Regulation of Aspergillus nidulans penicillin biosynthesis and penicillin biosynthesis genes acvA and ipnA by glucose.

    abstract::Expression of the Aspergillus nidulans penicillin biosynthesis genes acvA and ipnA, encoding delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase and isopenicillin N synthetase, respectively, was analyzed. The intergenic region carrying the divergently oriented promoters was fused in frame in both orientations ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brakhage AA,Browne P,Turner G

    更新日期:1992-06-01 00:00:00

  • O2R, a novel regulatory element mediating Rox1p-independent O(2) and unsaturated fatty acid repression of OLE1 in Saccharomyces cerevisiae.

    abstract::Fatty acid desaturation catalyzed by fatty acid desaturases requires molecular oxygen (O(2)). Saccharomyces cerevisiae cells derepress expression of OLE1 encoding Delta9 fatty acid desaturase under hypoxic conditions to allow more-efficient use of limited O(2). It has been proposed that aerobic conditions lead to repr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nakagawa Y,Sugioka S,Kaneko Y,Harashima S

    更新日期:2001-01-01 00:00:00

  • The legacy of HfrH: mutations in the two-component system CreBC are responsible for the unusual phenotype of an Escherichia coli arcA mutant.

    abstract::Strains derived from HfrH carrying the arcA2 null mutation exhibit a higher respiratory rate, enhanced glucose consumption, and a more-reduced intracellular redox state than arcA deletion mutants of a different lineage. The phenotype of the arcA2 mutants was due to the presence of a creC constitutive mutation introduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nikel PI,de Almeida A,Pettinari MJ,Méndez BS

    更新日期:2008-05-01 00:00:00

  • Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici.

    abstract::Lyons, A. J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and T. G. Pridham. Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici. J. Bacteriol. 83:370-380. 1962.-It is proposed that strain ATCC 3004 (IMRU 3004) be designated hen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: LYONS AJ Jr,PRIDHAM TG

    更新日期:1962-02-01 00:00:00

  • Role of PBP1 in cell division of Staphylococcus aureus.

    abstract::We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pereira SF,Henriques AO,Pinho MG,de Lencastre H,Tomasz A

    更新日期:2007-05-01 00:00:00

  • Control of EpsE, the phosphoglycosyltransferase initiating exopolysaccharide synthesis in Streptococcus thermophilus, by EpsD tyrosine kinase.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Minic Z,Marie C,Delorme C,Faurie JM,Mercier G,Ehrlich D,Renault P

    更新日期:2007-02-01 00:00:00

  • Mechanism of D-cycloserine action: alanine racemase from Escherichia coli W.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lambert MP,Neuhaus FC

    更新日期:1972-06-01 00:00:00

  • Transposition of Tn7 occurs at a single site on the Caulobacter crescentus chromosome.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ely B

    更新日期:1982-08-01 00:00:00

  • RNase III processing of intervening sequences found in helix 9 of 23S rRNA in the alpha subclass of Proteobacteria.

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    journal_title:Journal of bacteriology

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    authors: Evguenieva-Hackenberg E,Klug G

    更新日期:2000-09-01 00:00:00

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    pub_type: 杂志文章


    authors: Franzetti B,Sohlberg B,Zaccai G,von Gabain A

    更新日期:1997-02-01 00:00:00

  • Preferential selection of deletion mutations of the outer membrane lipoprotein gene of Escherichia coli by globomycin.

    abstract::Globomycin is an antibiotic which inhibits the processing of the prolipoprotein. Eighty globomycin-resistant mutants were independently isolated from Escherichia coli K-12 which had a deletion mutation in chromosomal lipoprotein gene (lpp), but contained a plasmid carrying the wild-type lpp gene. Twenty-six of the mut...

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    authors: Zwiebel LJ,Inukai M,Nakamura K,Inouye M

    更新日期:1981-01-01 00:00:00

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    journal_title:Journal of bacteriology

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    abstract::A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

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    pub_type: 杂志文章


    authors: Taylor BF,Campbell WL,Chinoy I

    更新日期:1970-05-01 00:00:00

  • Characterization and virulence properties of Erwinia chrysanthemi lipopolysaccharide-defective, phi EC2-resistant mutants.

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    journal_title:Journal of bacteriology

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    authors: Schoonejans E,Expert D,Toussaint A

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    abstract::The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...

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    pub_type: 杂志文章


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    更新日期:1990-04-01 00:00:00

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    pub_type: 杂志文章


    authors: Klainer AS,Gorbach S,Weinstein L

    更新日期:1967-08-01 00:00:00

  • Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans.

    abstract::The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate syn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


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    更新日期:1992-11-01 00:00:00

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    abstract::Oceaniovalibus guishaninsula, as a representative of a new genus within the family Rhodobacteraceae, was isolated from surface seawater that was sulfidic. Here, we present the draft genome sequence of the type strain, JLT2003(T). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tang K,Liu K,Jiao N

    更新日期:2012-12-01 00:00:00

  • Alterations in the cytoplasmic membrane proteins of various chlorate-resistant mutants of Escherichia coli.

    abstract::Chlorate-resistant mutants corresponding to each known genetic locus (chlA, chlB, chlC, chlD, chlE) were isolated from Escherichia coli K-12. All these mutants showed decreased amounts of membrane-bound nitrate reductase, cytochrome b, and formic dehydrogenase, but all had normal succinic dehydrogenase activity. Prote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


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    更新日期:1971-10-01 00:00:00

  • New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis.

    abstract::To isolate strains with new recA mutations that differentially affect RecA protein functions, we mutagenized in vitro the recA gene carried by plasmid mini-F and then introduced the mini-F-recA plasmid into a delta recA host that was lysogenic for prophage phi 80 and carried a lac duplication. By scoring prophage indu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dutreix M,Moreau PL,Bailone A,Galibert F,Battista JR,Walker GC,Devoret R

    更新日期:1989-05-01 00:00:00

  • A novel two-component signaling system that activates transcription of an enterohemorrhagic Escherichia coli effector involved in remodeling of host actin.

    abstract::Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for worldwide outbreaks of bloody diarrhea, hemorrhagic colitis, and life-threatening hemolytic uremic syndrome. After colonizing the large intestine, EHEC forms attaching and effacing (AE) lesions on intestinal epithelial cells. These lesions cause dest...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Reading NC,Torres AG,Kendall MM,Hughes DT,Yamamoto K,Sperandio V

    更新日期:2007-03-01 00:00:00

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    abstract::A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sonstein SA,Hammel JM,Bondi A

    更新日期:1971-08-01 00:00:00

  • Identification and characterization of alcR, a gene encoding an AraC-like regulator of alcaligin siderophore biosynthesis and transport in Bordetella pertussis and Bordetella bronchiseptica.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Beaumont FC,Kang HY,Brickman TJ,Armstrong SK

    更新日期:1998-02-01 00:00:00


    abstract::Wallace, W. R. (Louisiana State University, Baton Rouge), and G. T. Dimopoullos. Catalase activity in Anaplasma marginale. J. Bacteriol. 91:309-311. 1965.-Extracts of erythrocytes infected with Anaplasma marginale were found to contain more catalase activity than normal erythrocytic preparations. The increase in catal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1965-08-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Scott KP,Martin JC,Campbell G,Mayer CD,Flint HJ

    更新日期:2006-06-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schneider K,Skovran E,Vorholt JA

    更新日期:2012-06-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ripio MT,Domínguez-Bernal G,Lara M,Suárez M,Vazquez-Boland JA

    更新日期:1997-03-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sakurada K,Ohta T,Hasegawa M

    更新日期:1992-11-01 00:00:00