Isolation of Klebsiella aerogenes mutants cis-dominant for glutamine synthetase expression.

abstract：:We describe here the identification and characterization of two Listeria monocytogenes (Tn917-LTV3) relA and hpt transposon insertion mutants that were impaired in growth after attachment to a model surface. Both mutants were unable to accumulate (p)ppGpp in response to amino acid starvation, whereas the wild-type str...

journal_title：Journal of bacteriology

authors： Taylor CM,Beresford M,Epton HA,Sigee DC,Shama G,Andrew PW,Roberts IS

更新日期：2002-02-01 00:00:00

abstract：:Type 3 fimbriae are adhesive organelles found in enterobacterial pathogens. The fimbriae promote biofilm formation on biotic and abiotic surfaces; however, the exact identity of the receptor for the type 3 fimbriae adhesin, MrkD, remains elusive. We analyzed naturally occurring structural and functional variabilities ...

journal_title：Journal of bacteriology

authors： Stahlhut SG,Chattopadhyay S,Kisiela DI,Hvidtfeldt K,Clegg S,Struve C,Sokurenko EV,Krogfelt KA

更新日期：2013-12-01 00:00:00

abstract：:Gram-negative Aggregatibacter actinomycetemcomitans can be distinguished (based on the promoter structure of the leukotoxin operon) into JP2 and non-JP2 genotypes, with the former found to be more pathogenic than the latter. Here we report the first complete genome sequence of a serotype b non-JP2 strain of A. actinom...

journal_title：Journal of bacteriology

authors： Chen C,Kittichotirat W,Chen W,Downey JS,Bumgarner R

更新日期：2012-04-01 00:00:00

abstract：:The major cotransduction gap of the Escherichia coli chromosome extends from mini 31 to 34. We have inserted transposons through this gap which, by sequential transduction, link sbcA (min 29.8) with manA (min 35.7) and thus eliminate the gap. These results indicate that the length of DNA in the region, as measured by ...

journal_title：Journal of bacteriology

authors： Fouts KE,Barbour SD

更新日期：1982-01-01 00:00:00

abstract：:The segrosome is the nucleoprotein complex that mediates accurate plasmid segregation. In addition to its multifunctional role in segrosome assembly, the ParG protein of multiresistance plasmid TP228 is a transcriptional repressor of the parFG partition genes. ParG is a homodimeric DNA binding protein, with C-terminal...

journal_title：Journal of bacteriology

authors： Zampini M,Derome A,Bailey SE,Barillà D,Hayes F

更新日期：2009-06-01 00:00:00

abstract：:Transcription of the gyrA and gyrB genes, which encode the subunits of DNA gyrase, increases in response to DNA relaxation. Previous studies have shown that a small segment of DNA extending from the -10 consensus hexamer to the start of transcription encodes the sequence determinants for this response. In this study, ...

journal_title：Journal of bacteriology

authors： Straney R,Krah R,Menzel R

更新日期：1994-10-01 00:00:00

abstract：:The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in various organisms, of which IIAGlc of Escherichia coli is the...

journal_title：Journal of bacteriology

authors： Gunnewijk MG,Postma PW,Poolman B

更新日期：1999-01-01 00:00:00

abstract：:Erwinia chrysanthemi 3937 secretes several pectinolytic enzymes, among which eight isoenzymes of pectate lyases with an endo-cleaving mode (PelA, PelB, PelC, PelD, PelE, PelI, PelL, and PelZ) have been identified. Two exo-cleaving enzymes, the exopolygalacturonate lyase, PelX, and an exo-poly-alpha-D-galacturonosidase...

journal_title：Journal of bacteriology

authors： Shevchik VE,Kester HC,Benen JA,Visser J,Robert-Baudouy J,Hugouvieux-Cotte-Pattat N

更新日期：1999-03-01 00:00:00

abstract：:Mutations in any one of three genes, kdpA, -B, or -C, in Escherichia coli abolish the activity of Kdp, a multisubunit K+-ATPase that belongs to the P-type ATPase family of cation transporters. We found in this study that expression in vivo of a 135-amino-acid-long N-terminal fragment (KdpA'), less than one-quarter the...

journal_title：Journal of bacteriology

authors： Sardesai AA,Gowrishankar J

更新日期：2001-06-01 00:00:00

abstract：:A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the...

journal_title：Journal of bacteriology

authors： Neway JO,Switzer RL

更新日期：1983-08-01 00:00:00

abstract：:Certain group VIIIB transition metal compounds were found to inhibit cell division in Escherichia coli, causing marked filamentous growth. Gram-negative bacilli were the most sensitive to this effect, whereas gram-positive bacilli responded only at near-toxic levels of the metal. None of the cocci tested showed any ap...

journal_title：Journal of bacteriology

authors： Rosenberg B,Renshaw E,Vancamp L,Hartwick J,Drobnik J

更新日期：1967-02-01 00:00:00

abstract：:Degenerate oligonucleotides based on the published Escherichia coli glutamate decarboxylase (GAD) protein sequence were used in a polymerase chain reaction to generate a DNA probe for the E. coli GAD structural gene. Southern blots showed that there were two cross-hybridizing GAD genes, and both of these were cloned a...

journal_title：Journal of bacteriology

authors： Smith DK,Kassam T,Singh B,Elliott JF

更新日期：1992-09-01 00:00:00

abstract：:The bacterioferritin (BFR) of Escherichia coli K-12 is an iron-storage hemoprotein, previously identified as cytochrome b1. The bacterioferritin gene (bfr) has been cloned, sequenced, and located in the E. coli linkage map. Initially a gene fusion encoding a BFR-lambda hybrid protein (Mr 21,000) was detected by immuno...

journal_title：Journal of bacteriology

authors： Andrews SC,Harrison PM,Guest JR

更新日期：1989-07-01 00:00:00

abstract：:Two activites causing nitrite disappearance are found in extracts of Neurospora; one, inducible by nitrate or nitrite and present only in nitrite-utilizing strains, catalyze the stoichiometric reduction of nitrite to ammonia; the other, present in all strains under all conditions, causes the disappearance of nitrite t...

journal_title：Journal of bacteriology

authors： Chang HC,Mulkins GJ,Dyer JC,Sorger GJ

更新日期：1975-08-01 00:00:00

abstract：:Grunberg, E. (Hoffman-La Roche, Inc., Nutley, N.J.), and R. Cleeland. Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate. J. Bacteriol. 92:23-27. 1966.-Washed cell suspensions of Proteus mirabilis, under the proper conditions, stained well with fluorescein isothiocyanate w...

journal_title：Journal of bacteriology

authors： Grunberg E,Cleeland R

更新日期：1966-07-01 00:00:00

abstract：:In a search for genes that produce hypersensitivity to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used. One of the mutant strains obtained was sensitive to Cd2+ and Zn2+. Sequence analysis showed that the TnphoA insertion was located in the dsbA gene coding for a periplasmic prote...

journal_title：Journal of bacteriology

authors： Rensing C,Mitra B,Rosen BP

更新日期：1997-04-01 00:00:00

abstract：:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

journal_title：Journal of bacteriology

authors： Wood MS,Lory C,Lessie TG

更新日期：1990-04-01 00:00:00

abstract：:Procedures were developed to facilitate the identification of genes that belong to a given regulon and characterization of their responses to the regulator. The regulon controlled by the Escherichia coli leucine-responsive regulatory protein (Lrp) was studied by isolating random transcriptional fusions to lacZ, using ...

journal_title：Journal of bacteriology

authors： Bhagwat SP,Rice MR,Matthews RG,Blumenthal RM

更新日期：1997-10-01 00:00:00

abstract：:The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 pr...

journal_title：Journal of bacteriology

authors： Mercer AA,Loutit JS

更新日期：1979-10-01 00:00:00

abstract：:After frog auricles have been in contact with a suspension of bacteria or bacteria-free supernatant fluid, newly synthesized bacterial ribonucleic acid (RNA) is recovered in animal cells. It appears that the presence of bacterial deoxyribonucleic acid (DNA)-dependent RNA polymerase is necessary for the transcription o...

journal_title：Journal of bacteriology

authors： Stroun M,Anker P

更新日期：1973-04-01 00:00:00

abstract：:Bacterial chemotaxis is based on modulation of the probability to switch the direction of flagellar rotation. Responses to many stimuli are transduced by a two-component system via reversible phosphorylation of CheY, a small cytoplasmic protein that directly interacts with the switch complex at the flagellar motor. We...

journal_title：Journal of bacteriology

authors： Montrone M,Oesterhelt D,Marwan W

更新日期：1996-12-01 00:00:00

abstract：:A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. One pair of primers is capable of amplifying nearly full-length 16S ribosomal DNA (rDNA) ...

journal_title：Journal of bacteriology

authors： Weisburg WG,Barns SM,Pelletier DA,Lane DJ

更新日期：1991-01-01 00:00:00

abstract：:Staphylococcus lugdunensis is the only coagulase-negative Staphylococcus species with a locus encoding iron-regulated surface determinant (Isd) proteins. In Staphylococcus aureus, the Isd proteins capture heme from hemoglobin and transfer it across the wall to a membrane-bound transporter, which delivers it into the c...

journal_title：Journal of bacteriology

authors： Zapotoczna M,Heilbronner S,Speziale P,Foster TJ

更新日期：2012-12-01 00:00:00

abstract：UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

journal_title：Journal of bacteriology

authors： Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

更新日期：2015-07-01 00:00:00

abstract：:The essential gene efg, which complements ammonia-dependent growth (adgA) mutations in Rhodobacter capsulatus and is located at 38.1 min on the Escherichia coli chromosome, was found to code for NH3-dependent NAD synthetase. Crude extracts from a strain which overproduces the efg gene product contained up to 400 times...

journal_title：Journal of bacteriology

authors： Willison JC,Tissot G

更新日期：1994-06-01 00:00:00

abstract：:The stator proteins PomA and PomB form a complex that couples Na(+) influx to torque generation in the polar flagellar motor of Vibrio alginolyticus. This stator complex is anchored to an appropriate place around the rotor through a putative peptidoglycan-binding (PGB) domain in the periplasmic region of PomB (PomB(C)...

journal_title：Journal of bacteriology

authors： Li N,Kojima S,Homma M

更新日期：2011-08-01 00:00:00

abstract：:Leuconostoc mesenteroides subsp. mesenteroides is one of the most predominant lactic acid bacterial groups during kimchi fermentation. Here, we report the complete genome sequence of L. mesenteroides subsp. mesenteroides J18, which was isolated from kimchi. The genome of the strain consists of a 1,896,561-bp chromosom...

journal_title：Journal of bacteriology

authors： Jung JY,Lee SH,Lee SH,Jeon CO

更新日期：2012-02-01 00:00:00

abstract：:The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, ...

journal_title：Journal of bacteriology

authors： Futcher AB,Cox BS

更新日期：1984-01-01 00:00:00

abstract：:Xylella fastidiosa is a xylem-limited nonflagellated bacterium that causes economically important diseases of plants by developing biofilms that block xylem sap flow. How the bacterium is translocated downward in the host plant's vascular system against the direction of the transpiration stream has long been a puzzlin...

journal_title：Journal of bacteriology

authors： Meng Y,Li Y,Galvani CD,Hao G,Turner JN,Burr TJ,Hoch HC

更新日期：2005-08-01 00:00:00

abstract：:This paper describes the major transmutations of donor deoxyribonucleic acid (DNA) after uptake by competent Bacillus subtilis cells. Kinetic experiments confirm that after exposure to competent cells, donor DNA is converted to double-stranded fragments (DSF) which can be isolated as early as 30 s from the beginning o...

journal_title：Journal of bacteriology

authors： Davidoff-Abelson R,Dubnau D

更新日期：1973-10-01 00:00:00