Abstract:
:Mutations in any one of three genes, kdpA, -B, or -C, in Escherichia coli abolish the activity of Kdp, a multisubunit K+-ATPase that belongs to the P-type ATPase family of cation transporters. We found in this study that expression in vivo of a 135-amino-acid-long N-terminal fragment (KdpA'), less than one-quarter the length of native KdpA, was able to mediate an improvement in K+-limited growth rates in two different contexts, even in the absence of both KdpC and the ATPase subunit KdpB. The first context was when KdpA' was overexpressed in cells from a heterologous inducible promoter, and the second was when KdpA' was provided with a C-terminally altered extension (following a spontaneous genetic rearrangement). Our results suggest that KdpA' provides an incipient pathway for K+ translocation which can serve to transport K+ into the cells in response to the cytoplasmic membrane potential.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Sardesai AA,Gowrishankar Jdoi
10.1128/JB.183.11.3515-3520.2001keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
3515-20issue
11eissn
0021-9193issn
1098-5530journal_volume
183pub_type
杂志文章abstract::Magnetotactic bacteria synthesize specific organelles, the magnetosomes, which are membrane-enveloped crystals of the magnetic mineral magnetite (Fe(3)O(4)). The biomineralization of magnetite involves the uptake and intracellular accumulation of large amounts of iron. However, it is not clear how iron uptake and biom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00319-10
更新日期:2010-08-01 00:00:00
abstract::Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1144-1148.1977
更新日期:1977-06-01 00:00:00
abstract::Members of the genus Shewanella inhabit various environments; they are capable of synthesizing various types of low-melting-point fatty acids, including monounsaturated fatty acids (MUFA) and branched-chain fatty acids (BCFA) with and without eicosapentanoic acid (EPA). The genes involved in fatty acid synthesis in 15...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00498-08
更新日期:2009-04-01 00:00:00
abstract::Bishop, D. G. (University of Sydney, Sydney, Australia), and J. L. Still. Fatty acid metabolism in Serratia marcescens. J. Bacteriol. 82:370-375. 1961.-A study has been made of the oxidation of saturated fatty acids containing between two and 18 carbon atoms by whole cells of the bacterium, Serratia marcescens. This o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.3.370-375.1961
更新日期:1961-09-01 00:00:00
abstract::HypA and HypB are maturation proteins required for incorporation of nickel into the hydrogenase large subunit. To examine the functions of these proteins in nickel insertion, the hybF gene, which is a homolog of hypA essential for maturation of hydrogenases 1 and 2 from Escherichia coli, was overexpressed, and the pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.9.2603-2611.2004
更新日期:2004-05-01 00:00:00
abstract::The oral bacterium Streptococcus salivarius takes up lactose via a transporter called LacS that shares 95% identity with the LacS from Streptococcus thermophilus, a phylogenetically closely related organism. S. thermophilus releases galactose into the medium during growth on lactose. Expulsion of galactose is mediated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.23.6764-6772.2003
更新日期:2003-12-01 00:00:00
abstract::The molecular mechanisms of regulation of the genes involved in the biosynthesis of cysteine are poorly characterized in Bacillus subtilis and other gram-positive bacteria. In this study we describe the expression pattern of the B. subtilis cysH operon in response to sulfur starvation. A 6.1-kb polycistronic transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.20.5885-5892.2000
更新日期:2000-10-01 00:00:00
abstract::Salmonella enteritidis was injected intravenously, intraperitoneally, or subcutaneously into specific pathogen-free mice. The number of organisms in the blood, liver, spleen, peritoneal cavity, and draining inguinal lymph node was determined by daily enumeration. Opsonization of the organism with hyperimmune serum inc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.667-675.1969
更新日期:1969-02-01 00:00:00
abstract::The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.6.1867-1871.1997
更新日期:1997-03-01 00:00:00
abstract::The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.426-433.1982
更新日期:1982-02-01 00:00:00
abstract::Gough, Michael (Brown University, Providence, R.I.), and Seymour Lederberg. Methylated bases in the host-modified deoxyribonucleic acid of Escherichia coli and bacteriophage lambda. J. Bacteriol. 91:1460-1468. 1966.-The deoxyribonucleic acid (DNA) from strains of Escherichia coli and phage lambda was examined to deter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.4.1460-1468.1966
更新日期:1966-04-01 00:00:00
abstract::The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids, including an amino-terminal methionine. The protein was overexpressed in E. coli MC4100 by cloning the gene behind the l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.18.5971-5977.1992
更新日期:1992-09-01 00:00:00
abstract::Oceaniovalibus guishaninsula, as a representative of a new genus within the family Rhodobacteraceae, was isolated from surface seawater that was sulfidic. Here, we present the draft genome sequence of the type strain, JLT2003(T). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01874-12
更新日期:2012-12-01 00:00:00
abstract::Germination of dormant Bacillus subtilis spores with specific nutrient germinants is dependent on a number of inner membrane (IM) proteins, including (i) the GerA, GerB, and GerK germinant receptors (GRs) that respond to nutrient germinants; (ii) the GerD protein, essential for optimal GR function; and (iii) SpoVA pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00377-13
更新日期:2013-08-01 00:00:00
abstract::A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.495-501.1974
更新日期:1974-10-01 00:00:00
abstract::The emerging coverage of diverse habitats by metagenomic shotgun data opens new avenues of discovering functional novelty using computational tools. Here, we apply three different concepts for predicting novel functions within light-mediated microbial pathways in five diverse environments. Using phylogenetic approache...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.01084-08
更新日期:2009-01-01 00:00:00
abstract::Escherichia coli ssrA encodes a small stable RNA molecule, tmRNA, that has many diverse functions, including tagging abnormal proteins for degradation, supporting phage growth, and modulating the activity of DNA binding proteins. Here we show that ssrA plays a role in Salmonella enterica serovar Typhimurium pathogenes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.6.1558-1563.2000
更新日期:2000-03-01 00:00:00
abstract::Horizontal acquisition of novel chromosomal genes is considered to be a key process in the evolution of bacterial pathogens. However, the identification of gene presence or absence could be hindered by the inconsistencies in bacterial genome annotations. Here, we performed a cross-annotation of omnipresent core and mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00545-16
更新日期:2016-11-04 00:00:00
abstract::The Salmonella typhimurium pi-histidine episome was identified by Ames et al. (2) in an unstable partial revertant of a deletion mutation-containing strain, hisG203. HisG203 lacks the histidine operator, promoter, and part of the first structural gene. In this paper, we study some properties of pi factor and demonstra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.3.993-1000.1972
更新日期:1972-03-01 00:00:00
abstract::We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01387-06
更新日期:2007-01-01 00:00:00
abstract::IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00989-08
更新日期:2009-02-01 00:00:00
abstract::The cobB function of Salmonella typhimurium LT2 was defined in vivo as an alternative activity for the nicotinic acid mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme (CobT), which is involved in the assembly of the nucleotide loop of cobalamin in this bacterium (J. R. Trzebiatowski, G. A. O'T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.7016-7019.1996
更新日期:1996-12-01 00:00:00
abstract::We have isolated and determined the nucleotide sequence of a 1,626-base-pair fragment from R-plasmid R483 which encodes a trimethoprim-resistant dihydrofolate reductase. Analysis of the nucleotide sequence of this fragment revealed the presence of two open reading frames, each sufficient to encode polypeptides of appr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1001-1008.1983
更新日期:1983-09-01 00:00:00
abstract::In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.413-420.1972
更新日期:1972-10-01 00:00:00
abstract::Eleven conditional lethal dnaG(Ts) mutations were located by chemical cleavage of heteroduplexes formed between polymerase chain reaction-amplified DNAs from wild-type and mutant dnaG genes. This entailed end labeling one DNA strand of the heteroduplex, chemically modifying the strands with hydroxylamine or osmium tet...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.3.1268-1278.1991
更新日期:1991-02-01 00:00:00
abstract::Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3620-3630.1990
更新日期:1990-07-01 00:00:00
abstract::Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydroly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::Aronson, John N. (Arizona State University, Tempe), and Gerald R. Wermus. Effects of m-tyrosine on growth and sporulation of Bacillus species. J. Bacteriol. 90:38-46. 1965.-The aromatic amino acid analogue, dl-beta-(3-hydroxyphenyl)alanine (m-tyrosine), reduced sporulation of a strain of Bacillus subtilis to less than...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.1.38-46.1965
更新日期:1965-07-01 00:00:00
abstract::A catabolite-sensitive promoter was found to be involved in transcription of the heat shock regulatory gene rpoH encoding the sigma 32 protein. Expression of lacZ from the operon fusion, rpoHp-lacZ, was partially inhibited by glucose added to the broth medium. Dissection of the rpoH promoter region allowed us to local...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.5.2710-2715.1990
更新日期:1990-05-01 00:00:00
abstract::Phase-partitioning studies of the euryhaline bacterium Halomonas elongata demonstrated that the hydrophobic-hydrophilic nature of the cell surface changed as the bacterium grew in different NaCl concentrations. Mid-log-phase cells grown in a high (3.4 M) NaCl concentration were more hydrophilic than were cells grown i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.132-135.1988
更新日期:1988-01-01 00:00:00