16S ribosomal DNA amplification for phylogenetic study.

abstract：:The nucleotide sequence of a region located downstream of the nifB gene, both in the cyanobacterium Anabaena sp. strain PCC 7120 and in Rhizobium meliloti, has been determined. This region contains a gene (fdxN) whose predicted polypeptide product strongly resembles typical bacterial ferredoxins. Cyanobacteria have no...

journal_title：Journal of bacteriology

authors： Mulligan ME,Buikema WJ,Haselkorn R

更新日期：1988-09-01 00:00:00

abstract：:Purified Rickettsia prowazekii cells were able to transport L-proline. The influx of this amino acid had a Kt of 14 microM and a Vmax of about 64 pmol/min per mg of protein. Proline could not be transported by heat-killed or metabolically poisoned rickettsiae or at 0 degrees C. The uptake of proline was linear for alm...

journal_title：Journal of bacteriology

authors： Winkler HH,Daugherty RM

更新日期：1984-05-01 00:00:00

abstract：:The major cotransduction gap of the Escherichia coli chromosome extends from mini 31 to 34. We have inserted transposons through this gap which, by sequential transduction, link sbcA (min 29.8) with manA (min 35.7) and thus eliminate the gap. These results indicate that the length of DNA in the region, as measured by ...

journal_title：Journal of bacteriology

authors： Fouts KE,Barbour SD

更新日期：1982-01-01 00:00:00

abstract：:The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...

journal_title：Journal of bacteriology

authors： Clark JE,Ragsdale SW,Ljungdahl LG,Wiegel J

更新日期：1982-07-01 00:00:00

abstract：:Compared with the other class B multimodular penicillin- binding proteins (PBPs), the low-affinity PBP5 responsible for penicillin resistance in Enterococcus hirae R40, has an extended non-penicillin-binding module because of the presence of an approximately 110-amino-acid E-46(-)D-160 insert downstream from the membr...

journal_title：Journal of bacteriology

authors： Mollerach ME,Partoune P,Coyette J,Ghuysen JM

更新日期：1996-03-01 00:00:00

abstract：:Streptococcus bovis possesses two sugar phosphate phosphatases (Pases). Pase I is a soluble enzyme that is inhibited by the membrane fractions from lactose-grown cells and is insensitive to activation by S46D HPr, an analog of HPr(ser-P) of the sugar phosphotransferase system. Pase II is a membrane-associated enzyme t...

journal_title：Journal of bacteriology

authors： Cook GM,Ye JJ,Russell JB,Saier MH Jr

更新日期：1995-12-01 00:00:00

abstract：:Burkholderia thailandensis is a soil saprophyte that is closely related to the pathogen Burkholderia pseudomallei, the etiological agent of melioidosis in humans. The environmental niches and infection sites occupied by these bacteria are thought to contain only limited concentrations of oxygen, where they can generat...

journal_title：Journal of bacteriology

authors： Vitale A,Paszti S,Takahashi K,Toyofuku M,Pessi G,Eberl L

更新日期：2020-11-04 00:00:00

abstract：:Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and O282 genes were at si...

journal_title：Journal of bacteriology

authors： Stewart P,Kersten P,Vanden Wymelenberg A,Gaskell J,Cullen D

更新日期：1992-08-01 00:00:00

abstract：:The first generalized transducing bacteriophage reported for Bacillus megaterium has been characterized. Optimum conditions for lysate production and transduction procedures were established so that transducing frequencies of 8 x 10(-6) and higher are now possible. The phage, MP13, has a head diameter of 97 nm and a c...

journal_title：Journal of bacteriology

authors： Vary PS,Garbe JC,Franzen M,Frampton EW

更新日期：1982-03-01 00:00:00

abstract：:Yersinia enterocolitica transports YscM1 and YscM2 via the type III pathway, a mechanism that is required for the establishment of bacterial infections. Prior to host cell contact, YscM1 and YscM2 exert posttranscriptional regulation to inhibit expression of effector yop genes, which encode virulence factors that trav...

journal_title：Journal of bacteriology

authors： Cambronne ED,Sorg JA,Schneewind O

更新日期：2004-02-01 00:00:00

abstract：:The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive beta-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment. ...

journal_title：Journal of bacteriology

authors： Fields PI,Schreier HJ,Saha AL,Bernlohr RW

更新日期：1984-08-01 00:00:00

abstract：:A chromosomal insertion of transposon Tn917 partially restores the expression of protease and alpha-toxin activities to PM466, a genetically defined agr-null derivative of the wild-type Staphylococcus aureus strain RN6390. In co-transduction experiments, transposon-encoded erythromycin resistance and a protease- and a...

journal_title：Journal of bacteriology

authors： McNamara PJ,Milligan-Monroe KC,Khalili S,Proctor RA

更新日期：2000-06-01 00:00:00

abstract：:Izaki, Kazuo (University of Tokyo, Tokyo, Japan), Kan Kiuchi, and Kei Arima. Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bacteriol. 91:628-633. 1966.-A decrease in the uptake of tetracycline occurred concurrently with a rise in the level of resistanc...

journal_title：Journal of bacteriology

authors： Izaki K,Kiuchi K,Arima K

更新日期：1966-02-01 00:00:00

abstract：:YoeB is a bacterial toxin encoded by the yefM-yoeB toxin-antitoxin system found in various bacterial genomes. Here, we show that Staphylococcus aureus contains two YoeB homologues, both of which function as ribosome-dependent mRNA interferases to inhibit translation initiation in a manner identical to that of YoeB-ec ...

journal_title：Journal of bacteriology

authors： Yoshizumi S,Zhang Y,Yamaguchi Y,Chen L,Kreiswirth BN,Inouye M

更新日期：2009-09-01 00:00:00

abstract：:Although four of the five Salmonella pathogenicity islands (SPIs) have been characterized in detail for Salmonella enterica serovar Typhimurium, and the fifth has been characterized for Salmonella enterica serovar Dublin, there have been limited studies to examine them in detail in a range of pathogenic serovars of S....

journal_title：Journal of bacteriology

authors： Amavisit P,Lightfoot D,Browning GF,Markham PF

更新日期：2003-06-01 00:00:00

abstract：:Two nifA-like genes, designated anfA and vnfA, have been identified in Azotobacter vinelandii. The anfA gene is located upstream from the nitrogenase-3 structural gene cluster (anfHDGK) and is preceded by a sequence that is potentially part of a ntrA-dependent promoter. The product of anfA appears to be required for e...

journal_title：Journal of bacteriology

authors： Joerger RD,Jacobson MR,Bishop PE

更新日期：1989-06-01 00:00:00

abstract：:Strain C5.84 is a Tn5-751 insertion mutant of Aeromonas hydrophila which is unable to secrete extracellular proteins, instead accumulating them in the periplasm (B. Jiang and S.P. Howard, J. Bacteriol. 173:1241-1249, 1991). A 3.5-kb BglII fragment which complements this mutation was isolated from the chromosome of the...

journal_title：Journal of bacteriology

authors： Jahagirdar R,Howard SP

更新日期：1994-11-01 00:00:00

abstract：:Synthesis of heme, measured by incorporation of iron-59, and of bacteriochlorophyll was studied with wild-type and mutant strains of Rhodopseudomonas spheroides. The wild type formed heme from glycine and succinate at one-fortieth the rate of bacteriochlorophyll under anaerobic-light conditions. Added delta-aminolevul...

journal_title：Journal of bacteriology

authors： Lascelles J,Hatch TP

更新日期：1969-05-01 00:00:00

abstract：:The gene encoding the major NAD(P)H-flavin oxidoreductase (flavin reductase) of the luminous bacterium Vibrio fischeri ATCC 7744 was isolated by using synthetic oligonucleotide probes corresponding to the N-terminal amino acid sequence of the enzyme. Nucleotide sequence analysis suggested that the major flavin reducta...

journal_title：Journal of bacteriology

authors： Zenno S,Saigo K,Kanoh H,Inouye S

更新日期：1994-06-01 00:00:00

abstract：:Two Rhizobium phaseoli mutants, isolated previously by Tn5 mutagenesis, elicited infection threads which ceased development prematurely, usually within root hairs. These infection threads were wide, globular, and otherwise altered in morphology, compared with normal infection threads. Anatomy and division of the root ...

journal_title：Journal of bacteriology

authors： Noel KD,Vandenbosch KA,Kulpaca B

更新日期：1986-12-01 00:00:00

abstract：:D-Ribulose 1,5-bisphosphate (RuBP) carboxylase has been purified from the photosynthetic extreme halophile Ectothiorhodospira halophila. Despite a growth requirement for almost saturating sodium chloride in the medium, both crude and homogeneous preparations of RuBP carboxylase obtained from this organism were inhibit...

journal_title：Journal of bacteriology

authors： Tabita FR,McFadden BA

更新日期：1976-06-01 00:00:00

abstract：:The DNA binding protein WhiA is conserved in Gram-positive bacteria and is present in the genetically simple cell wall-lacking mycoplasmas. The protein shows homology to eukaryotic homing endonucleases but lacks nuclease activity. WhiA was first characterized in streptomycetes, where it regulates the expression of key...

journal_title：Journal of bacteriology

authors： Bohorquez LC,Surdova K,Jonker MJ,Hamoen LW

更新日期：2018-03-26 00:00:00

abstract：:E2 (dihydrolipoamide acetyltransferase) and E3 (dihydrolipoamide dehydrogenase) of the Clostridium magnum acetoin dehydrogenase enzyme system were copurified in a three-step procedure from acetoin-grown cells. The denatured E2-E3 preparation comprised two polypeptides with M(r)s of 49,000 and 67,000, respectively. Mic...

journal_title：Journal of bacteriology

authors： Krüger N,Oppermann FB,Lorenzl H,Steinbüchel A

更新日期：1994-06-01 00:00:00

abstract：:In cells of Leuconostoc oenos, the fermentation of L-malic acid generates both a transmembrane pH gradient, inside alkaline, and an electrical potential gradient, inside negative. In resting cells, the proton motive force ranged from -170 mV to -88 mV between pH 3.1 and 5.6 in the presence Of L-malate. Membrane potent...

journal_title：Journal of bacteriology

authors： Salema M,Lolkema JS,San Romão MV,Lourero Dias MC

更新日期：1996-06-01 00:00:00

abstract：:H-NS is an abundant nucleoid-associated protein involved in the maintenance of chromosomal architecture in bacteria. H-NS also has a role in silencing the expression of a variety of environmentally regulated genes during growth under nonpermissive conditions. In this study we demonstrate a role for H-NS in the negativ...

journal_title：Journal of bacteriology

authors： Nye MB,Pfau JD,Skorupski K,Taylor RK

更新日期：2000-08-01 00:00:00

abstract：:Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellula...

journal_title：Journal of bacteriology

authors： Seto H,Tomasz A

更新日期：1975-01-01 00:00:00

abstract：:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

journal_title：Journal of bacteriology

authors： Wood MS,Lory C,Lessie TG

更新日期：1990-04-01 00:00:00

abstract：:Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles. ...

journal_title：Journal of bacteriology

authors： Hoffmann HP,Szabo A,Avers CJ

更新日期：1970-10-01 00:00:00

abstract：:Ribulose 1,5-bisphosphate carboxylase-oxygenase (RuBPC-O) activity was greatly enhanced when Rhodopseudomonas sphaeroides was grown in a mineral salts medium supplied with 1.5% CO2 in hydrogen. Analysis of cell extracts by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that cells growing on 1.5% C...

journal_title：Journal of bacteriology

authors： Jouanneau Y,Tabita FR

更新日期：1986-02-01 00:00:00

abstract：UNLABELLED:TcpP and ToxR coordinately regulate transcription of toxT, the master regulator of numerous virulence factors in Vibrio cholerae. TcpP and ToxR are membrane-localized transcription factors, each with a periplasmic domain containing two cysteines. In ToxR, these cysteines form an intramolecular disulfide bond...

journal_title：Journal of bacteriology

authors： Morgan SJ,French EL,Thomson JJ,Seaborn CP,Shively CA,Krukonis ES

更新日期：2015-11-16 00:00:00