Abstract:
:Compared with the other class B multimodular penicillin- binding proteins (PBPs), the low-affinity PBP5 responsible for penicillin resistance in Enterococcus hirae R40, has an extended non-penicillin-binding module because of the presence of an approximately 110-amino-acid E-46(-)D-160 insert downstream from the membrane anchor. Expression of pbp5 genes lacking various parts of the insert-encoding region gives rise to proteins that are inert in terms of penicillin binding, showing that during folding of the PBP, the insert plays a role in the acquisition of a correct penicillin-binding configuration by the G-364(-)Q-678 carboxy-terminal module.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Mollerach ME,Partoune P,Coyette J,Ghuysen JMdoi
10.1128/jb.178.6.1774-1775.1996subject
Has Abstractpub_date
1996-03-01 00:00:00pages
1774-5issue
6eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2773-2782.1989
更新日期:1989-05-01 00:00:00
abstract::The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive beta-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.803-804.1984
更新日期:1984-08-01 00:00:00
abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.446-452.1965
更新日期:1965-08-01 00:00:00
abstract::Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5325-5329.1988
更新日期:1988-11-01 00:00:00
abstract::Irradiated L cells infected with Rickettsia tsutsugamushi were studied under the electron microscope to define the morphological growth pattern of the organism. For 2 days after inoculation, no rickettsiae were found either extra- or intracellularly; after 2 days multiple rickettsiae appeared within the host cells wit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.2.879-892.1983
更新日期:1983-05-01 00:00:00
abstract::The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.975-982.1989
更新日期:1989-02-01 00:00:00
abstract::Brubaker, Robert R. (Fort Detrick, Frederick, Md.) and Michael J. Surgalla. Pesticins. II. Production of pesticin I and II. J. Bacteriol. 84:539-545. 1962.-Pesticin I was separated from pesticin I inhibitor by ion-exchange chromatography of cell-free culture supernatant fluids and by acid precipitation of soluble prep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.3.539-545.1962
更新日期:1962-09-01 00:00:00
abstract::The gene coding for alveolysin, the thiol-activated toxin produced by Bacillus alvei, has been cloned by means of an oligonucleotide based on the known N-terminal sequence of the secreted protein. The complete nucleotide sequence of the gene has been determined. The deduced amino acid sequence of alveolysin shows that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.12.7301-7305.1990
更新日期:1990-12-01 00:00:00
abstract::To investigate the involvement of bacterial catalases of the symbiotic gram-negative bacterium Rhizobium meliloti in the development of Medicago-Rhizobium functional nodules, we cloned a putative kat gene by screening a cosmid library with a catalase-specific DNA probe amplified by PCR from the R. meliloti genome. Nuc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.6802-6809.1996
更新日期:1996-12-01 00:00:00
abstract::In Escherichia coli cultures maximally inhibited with chloramphenicol, formation of polypeptides still continued at a slow, constant rate for at least 90 min. The rate of leucine incorporation was reduced to 0.5%, but methionine was only reduced to 2%, suggesting that chains are normally initiated with methionine but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.2.582-589.1974
更新日期:1974-05-01 00:00:00
abstract::Attaching and effacing (AE) pathogens colonize the gut mucosa using a type three secretion system (T3SS) and a suite of effector proteins. The locus of enterocyte effacement (LEE) is the defining genetic feature of the AE pathogens, encoding the T3SS and the core effector proteins necessary for pathogenesis. Extensive...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00336-17
更新日期:2017-12-20 00:00:00
abstract::Glycerol trinitrate (GTN) reductase, which enables Agrobacterium radiobacter to utilize GTN and related explosives as sources of nitrogen for growth, was purified and characterized, and its gene was cloned and sequenced. The enzyme was a 39-kDa monomeric protein which catalyzed the NADH-dependent reductive scission of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.24.7796-7802.1997
更新日期:1997-12-01 00:00:00
abstract::The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich regio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.14.4520-4527.1993
更新日期:1993-07-01 00:00:00
abstract::Antibiotic-resistant Acinetobacter baumannii causes infections that are extremely difficult to treat. A significant role in these resistance profiles is attributed to multidrug efflux pumps, especially those belonging to the resistance-nodulation-cell division (RND) superfamily of transporters. In this study, we analy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00049-18
更新日期:2018-06-11 00:00:00
abstract::Phosphoenolpyruvate carboxylase (EC 4.1.1.31) from Azotobacter vinelandii, like the corresponding enzyme from other organisms, is activated by acetyl coenzyme A and inhibited by l-aspartate. Both modifiers affect primarily the affinity of the enzyme for phosphoenolpyruvate. This is the first enzyme with a strictly ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.1.31-36.1971
更新日期:1971-04-01 00:00:00
abstract::Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.913-917.1971
更新日期:1971-03-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is a gram-negative bacterium and a common commensal organism of the upper respiratory tract in humans. NTHi causes a number of diseases, including otitis media, sinusitis, conjunctivitis, exacerbations of chronic obstructive pulmonary disease, and bronchitis. During the course...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01040-06
更新日期:2007-02-01 00:00:00
abstract::Binding sites for the Escherichia coli protein integration host factor (IHF) include a set of conserved bases that can be summarized by the consensus sequence WATCAANNNNTTR (W is dA or dT, R is dA or dG, and N is any nucleotide). However, additional 5'-proximal bases, whose common feature is a high dA+dT content, are ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.10.2999-3006.1994
更新日期:1994-05-01 00:00:00
abstract::In Escherichia coli, the additon of ethanol resulted in the synthesis of an increased proportion of phospholipids containing two unsaturated fatty acids. The addition of hexanol resulted in the opposite effect, an increase in the proportion of monounsaturated molecular species. The alcohol-induced changes were quantit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.3.1040-1044.1980
更新日期:1980-06-01 00:00:00
abstract::Acinetobacter baumannii is a Gram-negative bacterium causing nosocomial infections worldwide. To gain quick insight into the molecular basis of biofilm formation in A. baumannii, we determined the complete genome sequence of A. baumannii strain 1656-2, which forms sturdy biofilm and is resistant to multiple drugs. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06109-11
更新日期:2011-11-01 00:00:00
abstract::We have utilized transposon mutagenesis to obtain insertional mutations in Providencia stuartii that activate the chromosomal aac(2')-la gene. Two closely linked mini-Tn5Cm insertions were obtained in a locus designated aarA, and a single insertion was obtained in a separate locus, aarC. Nucleotide sequence analysis, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.5140-5144.1994
更新日期:1994-08-01 00:00:00
abstract::The dermatophyte Trichophyton schoenleinii elaborates an extracellular collagenase which is specific for native collagen and gelatin and is separable from an enzyme which is active against Azocoll. The fungal enzyme differs from the bacterial collagenases in its acid pH and small molecular weight. In addition, the fun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.43-46.1968
更新日期:1968-01-01 00:00:00
abstract::Mutations in the tol-pal genes induce pleiotropic effects such as release of periplasmic proteins into the extracellular medium and hypersensitivity to drugs and detergents. Other outer membrane defective strains such as tolC, lpp, and rfa mutations are also altered in their outer membrane permeability. In this study,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.18.4872-4878.1998
更新日期:1998-09-01 00:00:00
abstract::In Salmonella enterica serovar Typhimurium, the stationary-phase sigma factor sigma(S) (RpoS) is required for virulence, stress resistance, biofilm formation, and development of the rdar morphotype. This morphotype is a multicellular behavior characterized by expression of the adhesive extracellular matrix components ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00033-06
更新日期:2006-06-01 00:00:00
abstract::Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.12.3329-3337.2002
更新日期:2002-06-01 00:00:00
abstract::The oral cavity is home to a wide variety of bacterial species, both commensal, such as various streptococcal species, and pathogenic, such as Porphyromonas gingivalis, one of the main etiological agents of periodontal disease. Our understanding of how these bacteria ultimately cause disease is highly dependent upon u...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00790-17
更新日期:2018-05-09 00:00:00
abstract::We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. In fact, the addi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.795-800.1986
更新日期:1986-06-01 00:00:00
abstract::Lipid synthesis was studied in both sporulating (diploid) and nonsporulating (haploid) cells of Saccharomyces cerevisiae. Two phases of lipid synthesis occur in diploid cells transferred to sporulation medium. Phase I, which occurs during the first 12 h of exposure to sporulation medium, was also observed in the haplo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.3.1158-1163.1973
更新日期:1973-06-01 00:00:00
abstract::Erythrobacter litoralis has been known as a bacteriochlorophyll a-containing, aerobic, anoxygenic, phototrophic bacterium. Here we announce the complete genome sequence of E. litoralis HTCC2594, which is devoid of phototrophic potential. E. litoralis HTCC2594, isolated by dilution-to-extinction culturing from seawater...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00026-09
更新日期:2009-04-01 00:00:00
abstract::Tn5 was used to generate mutants that were deficient in the dissimilatory reduction of nitrite for Pseudomonas sp. strain G-179, which contains a copper nitrite reductase. Three types of mutants were isolated. The first type showed a lack of growth on nitrate, nitrite, and nitrous oxide. The second type grew on nitrat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.20.6653-6658.1992
更新日期:1992-10-01 00:00:00