Importance of the E-46-D-160 polypeptide segment of the non-penicillin-binding module for the folding of the low-affinity, multimodular class B penicillin-binding protein 5 of Enterococus hirae.


:Compared with the other class B multimodular penicillin- binding proteins (PBPs), the low-affinity PBP5 responsible for penicillin resistance in Enterococcus hirae R40, has an extended non-penicillin-binding module because of the presence of an approximately 110-amino-acid E-46(-)D-160 insert downstream from the membrane anchor. Expression of pbp5 genes lacking various parts of the insert-encoding region gives rise to proteins that are inert in terms of penicillin binding, showing that during folding of the PBP, the insert plays a role in the acquisition of a correct penicillin-binding configuration by the G-364(-)Q-678 carboxy-terminal module.


J Bacteriol


Journal of bacteriology


Mollerach ME,Partoune P,Coyette J,Ghuysen JM




Has Abstract


1996-03-01 00:00:00












  • Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration.

    abstract::Microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. Here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (DSR) genes from closely and distantly related sulfate-reducing organisms to infer th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wagner M,Roger AJ,Flax JL,Brusseau GA,Stahl DA

    更新日期:1998-06-01 00:00:00

  • Genome sequence of Methyloversatilis universalis FAM5T, a methylotrophic representative of the order Rhodocyclales.

    abstract::Rhodocyclales are representative of versatile bacteria that are able to utilize a wide variety of organic compounds for growth, but only a few strains have been isolated in pure culture thus far. Here we present the genome sequence of Methyloversatilis universalis FAM5(T), the first cultivable methylotrophic member of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kittichotirat W,Good NM,Hall R,Bringel F,Lajus A,Médigue C,Smalley NE,Beck D,Bumgarner R,Vuilleumier S,Kalyuzhnaya MG

    更新日期:2011-09-01 00:00:00

  • Cloning, sequencing, and expression of bacteriophage BF23 late genes 24 and 25 encoding tail proteins.

    abstract::Two bacteriophage BF23 late genes, genes 24 and 25, were isolated on a 7.4-kb PstI fragment from the phage DNA, and their nucleotide sequences were determined. Gene 24 encodes a minor tail protein with the expected M(r) of 34,309, and gene 25 located 4 bp upstream of gene 24 encodes a major tail protein with the expec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nakayama S,Kaneko T,Ishimaru H,Moriwaki H,Mizobuchi K

    更新日期:1994-12-01 00:00:00

  • Perturbation of nifT expression in Klebsiella pneumoniae has limited effect on nitrogen fixation.

    abstract::In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Simon HM,Homer MJ,Roberts GP

    更新日期:1996-05-01 00:00:00

  • Independence of buoyant cell density and growth rate in Escherichia coli.

    abstract::The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kubitschek HE,Baldwin WW,Schroeter SJ,Graetzer R

    更新日期:1984-04-01 00:00:00

  • Purification and characterization of a molybdenum-pterin-binding protein (Mop) in Clostridium pasteurianum W5.

    abstract::A large-scale fractionation scheme purified the major molybdenum(Mo)-binding protein (Mop) from crude extracts of Clostridium pasteurianum, with a 10 and 0.2% yield of Mo and protein, respectively. The apparent molecular weight of the purified molybdoprotein is 5,700, as determined by sodium dodecyl sulfate-polyacryla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hinton SM,Merritt B

    更新日期:1986-11-01 00:00:00

  • Surface viscoelasticity of individual gram-negative bacterial cells measured using atomic force microscopy.

    abstract::The cell envelope of gram-negative bacteria is responsible for many important biological functions: it plays a structural role, it accommodates the selective transfer of material across the cell wall, it undergoes changes made necessary by growth and division, and it transfers information about the environment into th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vadillo-Rodriguez V,Beveridge TJ,Dutcher JR

    更新日期:2008-06-01 00:00:00

  • Population genetic analysis of Helicobacter pylori by multilocus enzyme electrophoresis: extensive allelic diversity and recombinational population structure.

    abstract::Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes encoding metabolic housekeeping enzymes by multilocus enzyme electrophoresis. Seventy-three distinct allele profiles, r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Go MF,Kapur V,Graham DY,Musser JM

    更新日期:1996-07-01 00:00:00


    abstract::Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1964-11-01 00:00:00

  • Dynamics of FtsZ assembly during sporulation in Streptomyces coelicolor A3(2).

    abstract::FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Grantcharova N,Lustig U,Flärdh K

    更新日期:2005-05-01 00:00:00

  • Carotenoids acts as reinforcers of the Acholeplasma laidlawii lipid bilayer.

    abstract::Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rottem S,Markowitz O

    更新日期:1979-12-01 00:00:00

  • Assembly site of bacteriophage f1 corresponds to adhesion zones between the inner and outer membranes of the host cell.

    abstract::Morphogenesis of the filamentous bacteriophage f1 occurred at adhesion zones between the inner and outer membranes of the host cell. Quantitation of adhesion zones in cells infected with mutant phage strains suggested that the phage gene I protein may be involved in the formation of adhesion zones for phage assembly. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lopez J,Webster RE

    更新日期:1985-09-01 00:00:00

  • Lysine catabolism in Streptomyces spp. is primarily through cadaverine: beta-lactam producers also make alpha-aminoadipate.

    abstract::Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces lividans. This pathway is used when lysine is supplied as the sole source of nitrogen for the organism. A second pathway...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Madduri K,Stuttard C,Vining LC

    更新日期:1989-01-01 00:00:00

  • Phospholipase C regulatory mutation of Pseudomonas aeruginosa that results in constitutive synthesis of several phosphate-repressible proteins.

    abstract::We describe here a new mutant of Pseudomonas aeruginosa PAO, strain D10C (genotype plcB), which produces phospholipase C and alkaline phosphatase constitutively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the extracellular proteins produced by this mutant in high- and low-Pi media revealed that the m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gray GL,Berka RM,Vasil ML

    更新日期:1982-06-01 00:00:00

  • Catabolism of indole-3-acetic acid and 4- and 5-chloroindole-3-acetic acid in Bradyrhizobium japonicum.

    abstract::Some strains of Bradyrhizobium japonicum have the ability to catabolize indole-3-acetic acid. Indoleacetic acid (IAA), 4-chloro-IAA (4-Cl-IAA), and 5-Cl-IAA were metabolized to different extents by strains 61A24 and 110. Metabolites were isolated and analyzed by high-performance liquid chromatography and conventional ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jensen JB,Egsgaard H,Van Onckelen H,Jochimsen BU

    更新日期:1995-10-01 00:00:00

  • An extracellular factor regulates expression of sdiA, a transcriptional activator of cell division genes in Escherichia coli.

    abstract::The sdiA gene codes for a protein that regulates expression of the ftsQAZ cluster of essential cell division genes of Escherichia coli. SdiA up-regulates the ftsQ2p promoter that initiates transcription into the ftsQAZ cluster. In this paper, we report that expression of sdiA is itself regulated by a factor that is re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: García-Lara J,Shang LH,Rothfield LI

    更新日期:1996-05-01 00:00:00

  • Regulation at the phosphoenolpyruvate branchpoint in Azotobacter vinelandii: phosphoenolpyruvate carboxylase.

    abstract::Phosphoenolpyruvate carboxylase (EC from Azotobacter vinelandii, like the corresponding enzyme from other organisms, is activated by acetyl coenzyme A and inhibited by l-aspartate. Both modifiers affect primarily the affinity of the enzyme for phosphoenolpyruvate. This is the first enzyme with a strictly ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Liao CL,Atkinson DE

    更新日期:1971-04-01 00:00:00

  • Homology among Escherichia coli K1 and K92 polysialytransferases.

    abstract::The neuS-encoded polysialytransferase (polyST) in Escherichia coli K1 catalyzes synthesis of polysialic acid homopolymers composed of unbranched sialyl alpha 2,8 linkages. Subcloning and complementation experiments showed that the K1 neuS was functionally interchangeable with the neuS from E. coli K92 (S. M. Steenberg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vimr ER,Bergstrom R,Steenbergen SM,Boulnois G,Roberts I

    更新日期:1992-08-01 00:00:00

  • Novel cellular organization in a gliding mycoplasma, Mycoplasma insons.

    abstract::Mycoplasmas that are known to exhibit gliding motility possess a differentiated tip structure. This polar organelle mediates cytadherence and gliding motor activity and contains a cytoskeleton-like component that provides structural support. Here, we describe gliding motility and a unique cytoskeleton in Mycoplasma in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Relich RF,Friedberg AJ,Balish MF

    更新日期:2009-08-01 00:00:00

  • Augmented expression of polysaccharide intercellular adhesin in a defined Staphylococcus epidermidis mutant with the small-colony-variant phenotype.

    abstract::While coagulase-negative staphylococci (CoNS), with their ability to form a thick, multilayered biofilm on foreign bodies, have been identified as the major cause of implant-associated infections, no data are available about biofilm formation by staphylococcal small-colony variants (SCVs). In the past years, a number ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Al Laham N,Rohde H,Sander G,Fischer A,Hussain M,Heilmann C,Mack D,Proctor R,Peters G,Becker K,von Eiff C

    更新日期:2007-06-01 00:00:00

  • Alanylclavam biosynthetic genes are clustered together with one group of clavulanic acid biosynthetic genes in Streptomyces clavuligerus.

    abstract::Streptomyces clavuligerus produces at least five different clavam metabolites, including clavulanic acid and the methionine antimetabolite, alanylclavam. In vitro transposon mutagenesis was used to analyze a 13-kb region upstream of the known paralogue gene cluster. The paralogue cluster includes one group of clavulan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zelyas NJ,Cai H,Kwong T,Jensen SE

    更新日期:2008-12-01 00:00:00

  • Evidence for a type III secretion system in Aeromonas salmonicida subsp. salmonicida.

    abstract::Aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. We have screened this bacterium with a broad-host-range probe directed against yscV, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type III ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Burr SE,Stuber K,Wahli T,Frey J

    更新日期:2002-11-01 00:00:00

  • Method for determining whether a gene of Escherichia coli is essential: application to the polA gene.

    abstract::We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Joyce CM,Grindley ND

    更新日期:1984-05-01 00:00:00

  • Identification of the mutation responsible for the temperature-sensitive lipopolysaccharide O-antigen defect in the Pseudomonas aeruginosa cystic fibrosis isolate 2192.

    abstract::Pseudomonas aeruginosa in the lungs of cystic fibrosis (CF) patients is characterized by a series of genotypic and phenotypic changes that reflect the transition from acute to chronic infection. These include the overproduction of the exopolysaccharide alginate and the loss of complete lipopolysaccharide (LPS). LPS is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Davis MR Jr,Muszynski A,Lollett IV,Pritchett CL,Carlson RW,Goldberg JB

    更新日期:2013-04-01 00:00:00

  • Functional expression of plastid allophycocyanin genes in a cyanobacterium.

    abstract::In Cyanophora paradoxa, the allophycocyanin apoprotein subunits, alpha and beta, are encoded in the cyanelle (plastid) genome. These genes were transferred to the cyanobacterium Synechococcus sp. PCC 7002 on a plasmid replicon. Phycobilisomes isolated from transformed cyanobacteria were found to contain C. paradoxa al...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: de Lorimier R,Guglielmi G,Bryant DA,Stevens SE Jr

    更新日期:1987-05-01 00:00:00

  • 3-hydroxypropionyl-coenzyme A dehydratase and acryloyl-coenzyme A reductase, enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in the Sulfolobales.

    abstract::A 3-hydroxypropionate/4-hydroxybutyrate cycle operates in autotrophic CO(2) fixation in various Crenarchaea, as studied in some detail in Metallosphaera sedula. This cycle and the autotrophic 3-hydroxypropionate cycle in Chloroflexus aurantiacus have in common the conversion of acetyl-coenzyme A (CoA) and two bicarbon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Teufel R,Kung JW,Kockelkorn D,Alber BE,Fuchs G

    更新日期:2009-07-01 00:00:00

  • An Extended Cyclic Di-GMP Network in the Predatory Bacterium Bdellovibrio bacteriovorus.

    abstract:UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rotem O,Nesper J,Borovok I,Gorovits R,Kolot M,Pasternak Z,Shin I,Glatter T,Pietrokovski S,Jenal U,Jurkevitch E

    更新日期:2015-08-31 00:00:00

  • Biochemical characterization of UDP-Gal:GlcNAc-pyrophosphate-lipid β-1,4-Galactosyltransferase WfeD, a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating-unit synthesis.

    abstract::The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Xu C,Liu B,Hu B,Han Y,Feng L,Allingham JS,Szarek WA,Wang L,Brockhausen I

    更新日期:2011-01-01 00:00:00

  • Crystalline inclusions of bacterium 22M.

    abstract::Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kim KS,Barksdale L

    更新日期:1969-06-01 00:00:00

  • Characterization of CMP-N-acetylneuraminic acid synthetase of group B streptococci.

    abstract::The capsular polysaccharide is a critical virulence factor for group B streptococci associated with human infections, yet little is known about capsule biosynthesis. We detected CMP-Neu5Ac synthetase, the enzyme which activates N-acetylneuraminic acid (Neu5Ac, or sialic acid) for transfer to the nascent capsular polys...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Haft RF,Wessels MR

    更新日期:1994-12-01 00:00:00