Two nifA-like genes required for expression of alternative nitrogenases by Azotobacter vinelandii.

abstract：:The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escherichia coli lacZ was forced to form tandem repeats by the selection of clones tha...

journal_title：Journal of bacteriology

authors： Piggot PJ,Curtis CA

更新日期：1987-03-01 00:00:00

abstract：:Cleared lysates of 13 species of filamentous cyanobacteria were examined for the presence of extrachromosomal DNA by using agarose gel electrophoresis and ethidium bromide staining. Seven of the 13 species contained extrachromosomal covalently closed circular DNA, and all but 1 species contained multiple elements. The...

journal_title：Journal of bacteriology

authors： Simon RD

更新日期：1978-10-01 00:00:00

abstract：:Vacuoles project streams of vesicles and membranous tubules into the yeast bud where they fuse, founding the daughter cell organelle, vac5-1, which encodes a truncated form of the Pho80 cyclin, inhibits normal vacuole inheritance. An in vitro inheritance assay which measures the fusion of vacuoles serves as a model fo...

journal_title：Journal of bacteriology

authors： Nicolson T,Conradt B,Wickner W

更新日期：1996-07-01 00:00:00

abstract：:We examined the predacious gram-negative bacterium Bdellovibrio bacteriovorous 109J and free-living strains 109J-A1 and 109J-KA1 derived therefrom for penicillin-binding proteins (PBPs). We compared their PBPs with those of the host bacterium, Escherichia coli, and with those of a facultatively predacious bdellovibrio...

journal_title：Journal of bacteriology

authors： Park JT,Mahadevan S

更新日期：1988-08-01 00:00:00

abstract：:Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of c...

journal_title：Journal of bacteriology

authors： Sonstein SA,Baldwin JN

更新日期：1972-01-01 00:00:00

abstract：:We have devised a coupled radiochemical assay for detecting phosphatidylglycerolphosphate (PGP) phosphatase activity in Escherichia coli colonies immobilized on filter paper. There appeared to be at least two enzymes capable of dephosphorylating PGP, as judged by the characterization of mutations in two genes designat...

journal_title：Journal of bacteriology

authors： Icho T,Raetz CR

更新日期：1983-02-01 00:00:00

abstract：:We describe the isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. These mutations, designated spr(Am), were isolated and characterized in a lexA tif sfi genetic background. They abolished the sensitivity of the strain to UV light and resulted in high rates of synthesis of rec...

journal_title：Journal of bacteriology

authors： Pacelli LZ,Edmiston SH,Mount DW

更新日期：1979-01-01 00:00:00

abstract：:The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated in large part by a small noncoding RNA named FsrA. F...

journal_title：Journal of bacteriology

authors： Smaldone GT,Revelles O,Gaballa A,Sauer U,Antelmann H,Helmann JD

更新日期：2012-05-01 00:00:00

abstract：:A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, su...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Bukald NE

更新日期：1973-01-01 00:00:00

abstract：:Faithful DNA replication involves the removal of RNA residues from genomic DNA prior to the ligation of nascent DNA fragments in all living organisms. Because the physiological roles of archaeal type 2 RNase H are not fully understood, the substrate structure requirements for the detection of RNase H activity need fur...

journal_title：Journal of bacteriology

authors： Le Laz S,Le Goaziou A,Henneke G

更新日期：2010-07-01 00:00:00

abstract：:Cells of Haemophilus influenzae strain Rd competent for genetic transformation irreversibly bound approximately five molecular fragments of H. influenzae deoxyribonucleic acid (DNA) per cell; under identical conditions, DNA derived from Escherichia coli B was not taken up (<1 molecule per 50 cells). Similarly, DNA fro...

journal_title：Journal of bacteriology

authors： Scocca JJ,Poland RL,Zoon KC

更新日期：1974-05-01 00:00:00

abstract：:A noncomposite transposon, designated Tn5541, was isolated from strain Fd33 of the filamentous cyanobacterium Fremyella diplosiphon UTEX 481. Sequence analysis showed that Tn5541 is structurally and genetically very similar to Tn5469, which is also endogenous to F. diplosiphon. Both Tn5469 and Tn5541 encode homologous...

journal_title：Journal of bacteriology

authors： Schaefer MR,Kahn K

更新日期：1998-11-01 00:00:00

abstract：:The Arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 secretes an antifreeze protein (AFP) that promotes survival at subzero temperatures. The AFP is unusual in that it also exhibits a low level of ice nucleation activity. A DNA fragment with an open reading frame encoding 473 amino acids was clon...

journal_title：Journal of bacteriology

authors： Muryoi N,Sato M,Kaneko S,Kawahara H,Obata H,Yaish MW,Griffith M,Glick BR

更新日期：2004-09-01 00:00:00

abstract：:The staphylococcal virulon is activated by the density-sensing agr system, which is autoinduced by a short peptide (autoinducing peptide [AIP]) processed from a propeptide encoded by agrD. A central segment of the agr locus, consisting of the C-terminal two-thirds of AgrB (the putative processing enzyme), AgrD, and th...

journal_title：Journal of bacteriology

authors： Jarraud S,Lyon GJ,Figueiredo AM,Lina G,Vandenesch F,Etienne J,Muir TW,Novick RP

更新日期：2000-11-01 00:00:00

abstract：:An unusual xylose isomerase produced by Thermoanaerobacterium strain JW/SL-YS 489 was purified 28-fold to gel electrophoretic homogeneity, and the biochemical properties were determined. Its pH optimum distinguishes this enzyme from all other previously described xylose isomerases. The purified enzyme had maximal acti...

journal_title：Journal of bacteriology

authors： Liu SY,Wiegel J,Gherardini FC

更新日期：1996-10-01 00:00:00

abstract：:Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite. ...

journal_title：Journal of bacteriology

authors： Berg CM,Rossi JJ,Coleman JV,Conlon M

更新日期：1975-03-01 00:00:00

abstract：:The serotype 5 capsule gene cluster of Staphylococcus aureus comprises 16 genes (cap5A through cap5P), but little is known about how the putative gene products function in capsule biosynthesis. We propose that the N-acetylmannosaminuronic acid (ManNAcA) component of the S. aureus serotype 5 capsular polysaccharide (CP...

journal_title：Journal of bacteriology

authors： Kiser KB,Bhasin N,Deng L,Lee JC

更新日期：1999-08-01 00:00:00

abstract：:The relative stability of membrane proteins in Escherichia coli was investigated to determine whether these proteins are degraded at heterogeneous rates and, if so, whether the degradative rates are correlated with the sizes or charges of the proteins. Cells growing in a glucose-limited chemostat with a generation tim...

journal_title：Journal of bacteriology

authors： Schroer DW,St John AC

更新日期：1981-05-01 00:00:00

abstract：:The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered Escherichia coli. Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of Switching frequency and bias centering at...

journal_title：Journal of bacteriology

authors： Montrone M,Eisenbach M,Oesterhelt D,Marwan W

更新日期：1998-07-01 00:00:00

abstract：:Conditions have been established for the preparation of spheroplasts of Saccharomyces cerevisiae which are able to increase their net content of protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), several-fold upon incubation in a medium stabilized with 1 m sorbitol. The rate of RNA and protein synthesis...

journal_title：Journal of bacteriology

authors： Hutchison HT,Hartwell LH

更新日期：1967-11-01 00:00:00

abstract：:Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2...

journal_title：Journal of bacteriology

authors： Chang YF,Feingold DS

更新日期：1970-04-01 00:00:00

abstract：:The pelA and pelC genes from Erwinia chrysanthemi EC16 were sequenced and overexpressed in Escherichia coli cells. These genes and two others from the same strain that were characterized previously encode catalytically related pectate lyase proteins that are involved with the maceration and soft-rotting of plant tissu...

journal_title：Journal of bacteriology

authors： Tamaki SJ,Gold S,Robeson M,Manulis S,Keen NT

更新日期：1988-08-01 00:00:00

abstract：:Postembedding staining of intracellular carbohydrates on thin sections of Staphylococcus aureus was studied by the silver methenamine and the wheat germ agglutinin-gold techniques. Staining of silver grains was observed on both the cell wall and the cross wall. The staining was interpreted to be due to teichoic acid. ...

journal_title：Journal of bacteriology

authors： Morioka H,Tachibana M,Suganuma A

更新日期：1987-03-01 00:00:00

abstract：:A systematic analysis of the type III secretion (T3S) genes of Pseudomonas aeruginosa strain PAO1 revealed that they are under quorum-sensing control. This observation was supported by the down-regulation of the T3S regulon in the presence of RhlR-C4HSL and the corresponding advanced secretion of ExoS in a rhlI mutant...

journal_title：Journal of bacteriology

authors： Bleves S,Soscia C,Nogueira-Orlandi P,Lazdunski A,Filloux A

更新日期：2005-06-01 00:00:00

abstract：:Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...

journal_title：Journal of bacteriology

authors： Vela GR,Cagle GD,Holmgren PR

更新日期：1970-11-01 00:00:00

abstract：:A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the...

journal_title：Journal of bacteriology

authors： Neway JO,Switzer RL

更新日期：1983-08-01 00:00:00

abstract：:We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

journal_title：Journal of bacteriology

authors： Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

更新日期：2000-10-01 00:00:00

abstract：:Using a system of chromatography through columns of DEAE-Bio-Gel, HTP-Bio-Gel, and CM-Bio-Gel, we isolated and characterized six different (1 leads to 3)-beta-glucanases from cell wall autolysates and cell extracts of Saccharomyces cerevisiae haploid strain 2180B. These enzymes were designated glucanases I, II, IIIA, ...

journal_title：Journal of bacteriology

authors： Hien NH,Fleet GH

更新日期：1983-12-01 00:00:00

abstract：:Yersinia pestis is an important human pathogen that is maintained in flea-rodent enzootic cycles in many parts of the world. During its life cycle, Y. pestis senses host-specific environmental cues such as temperature and regulates gene expression appropriately to adapt to the insect or mammalian host. For example, Y....

journal_title：Journal of bacteriology

authors： Rebeil R,Ernst RK,Jarrett CO,Adams KN,Miller SI,Hinnebusch BJ

更新日期：2006-02-01 00:00:00

abstract：:Burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with ∼2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these B. pseudomallei genes, which can be discerned through evolutionar...

journal_title：Journal of bacteriology

authors： Singh AP,Lai SC,Nandi T,Chua HH,Ooi WF,Ong C,Boyce JD,Adler B,Devenish RJ,Tan P

更新日期：2013-12-01 00:00:00