Specificity in deoxyribonucleic acid uptake by transformable Haemophilus influenzae.

Abstract:

:Cells of Haemophilus influenzae strain Rd competent for genetic transformation irreversibly bound approximately five molecular fragments of H. influenzae deoxyribonucleic acid (DNA) per cell; under identical conditions, DNA derived from Escherichia coli B was not taken up (<1 molecule per 50 cells). Similarly, DNA from Xenopus laevis was not taken up by competent H. influenzae. Of the heterologous DNAs tested, only DNA from H. parainfluenzae interfered with the uptake of H. influenzae DNA, as judged by competition experiments employing either DNA binding or genetic transformation as the test system. The extracellular heterologous DNA did not suffer either single- or double-strand breakage upon exposure to competent H. influenzae.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Scocca JJ,Poland RL,Zoon KC

doi

10.1128/JB.118.2.369-373.1974

subject

Has Abstract

pub_date

1974-05-01 00:00:00

pages

369-73

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

118

pub_type

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