Abstract:
:The WO-1 strain of Candida albicans is capable of alternating between two highly distinct yeast cell types termed white and opaque (E. H. A. Rikkerrink, B. B. Magee, and P. T. Magee, J. Bacteriol. 170:895-899, 1988; B. Slutsky, M. Staebell, J. Anderson, L. Risen, M. Pfaller, and D. R. Soll, J. Bacteriol. 169:189-197, 1987). We have isolated WO-1 mutants that show a marked deficiency at being able to switch from the white form to the opaque form under conditions normally favorable for this transition. Pulsed-field electrophoresis demonstrated that one of the initial two spontaneous nonswitching mutants lacked the smallest chromosome that is normally present in WO-1. The availability of a WO-1 derivative whose only functional ADE2 gene is located on this small chromosome made possible, through the induction of chromosome nondisjunction, the isolation of numerous new mutants missing this chromosome as well as mutants containing two copies of the chromosome. Mutants missing the smallest chromosome showed a greatly diminished ability to produce opaque sectors and to produce germ tubes in the presence of human serum. Mutants containing two copies of the small chromosome showed an increased ability to produce germ tubes. These results indicate that this small chromosome carries one or more genes involved in both the white-opaque switch and the yeast-hyphal switch.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
McEachern MJ,Hicks JBdoi
10.1128/jb.173.23.7436-7442.1991subject
Has Abstractpub_date
1991-12-01 00:00:00pages
7436-42issue
23eissn
0021-9193issn
1098-5530journal_volume
173pub_type
杂志文章abstract::Sixteen independent Azorhizobium sesbaniae ORS571 vector insertion (Vi) mutants defective in ammonium assimilation (Asm-) were selected; genomic DNA sequences flanking the insertion endpoints were cloned directly. Resulting recombinant plasmids were used to identify, by hybridization, corresponding wild-type DNA seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1197-1204.1988
更新日期:1988-03-01 00:00:00
abstract::Bacterial chemotaxis is based on modulation of the probability to switch the direction of flagellar rotation. Responses to many stimuli are transduced by a two-component system via reversible phosphorylation of CheY, a small cytoplasmic protein that directly interacts with the switch complex at the flagellar motor. We...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.6882-6887.1996
更新日期:1996-12-01 00:00:00
abstract::Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3448-3452.1998
更新日期:1998-07-01 00:00:00
abstract::Transcription of an aqpZ-lac fusion in a single copy on the Escherichia coli chromosome increased as cells entered the stationary growth phase. This was true in a variety of media, and increased transcription in enriched medium required the RpoS sigma factor. Expression of the aqpZ-lac fusion was not affected by up- o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.15.4304-4307.2002
更新日期:2002-08-01 00:00:00
abstract::In Rhodobacter sphaeroides, MreB, MreC, MreD, PBP2, and RodA are encoded at the same locus. The localizations of PBP2, MreB, and MreC, which have all been implicated in the synthesis of the peptidoglycan layer, were investigated under different growth conditions to gain insight into the relationships between these pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.5.1691-1700.2006
更新日期:2006-03-01 00:00:00
abstract::The cheA locus of Escherichia coli encodes two similar proteins, CheAL (654 amino acids) and CheAS (557 amino acids), which are made by initiating translation from different in-frame start sites [start(L) and start(S)]. CheAL plays an essential role in chemotactic signaling. It autophosphorylates at a histidine residu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2713-2720.1995
更新日期:1995-05-01 00:00:00
abstract:UNLABELLED:Cell shape has been suggested to play an important role in the regulation of bacterial attachment to surfaces and the formation of communities associated with surfaces. We found that a cardiolipin synthase (Δcls) mutant of the rod-shaped bacterium Rhodobacter sphaeroides--in which synthesis of the anionic, h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00420-15
更新日期:2015-11-01 00:00:00
abstract::Colonies of S. mutans OMZ61, Streptococcus sp. D182, Staphylococcus aureus Oxford NCTC 6571, and Candida albicans type A, MRL 3153 were grown on various media. Cubes of agar bearing two to three colonies were excised and processed for scanning electron microscopy. The characteristic shape of the colonies was seen when...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.2.902-909.1970
更新日期:1970-11-01 00:00:00
abstract::The group A streptococcus (GAS) Streptococcus pyogenes is known to cause self-limiting purulent infections in humans. The role of GAS pili in host cell adhesion and biofilm formation is likely fundamental in early colonization. Pilus genes are found in the FCT (fibronectin-binding protein, collagen-binding protein, an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06283-11
更新日期:2012-02-01 00:00:00
abstract::Synchronized cultures of Anacystis nidulans (Synechococcus PCC 6301), an obligate phototroph, are obtained by incubating exponential cultures in the dark for 12 to 16 h. A temporal and sequential order of macromolecular synthesis is observed within the cell division cycle of a synchronously dividing culture in the lig...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1315-1321.1983
更新日期:1983-03-01 00:00:00
abstract::The potassium, calcium, magnesium, and zinc ion content of cells in exponential and synchronously growing cultures of Escherichia coli B/r was determined with an X-ray fluorescence spectrometer and an atomic absorption spectrophotometer. Cellular potassium, calcium, and magnesium content increased smoothly during the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.3.1089-1095.1976
更新日期:1976-06-01 00:00:00
abstract::A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restricti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.1087-1092.1994
更新日期:1994-02-01 00:00:00
abstract::Vibrio cholerae is a highly motile bacterium which possesses a single polar flagellum as a locomotion organelle. Motility is thought to be an important factor for the virulence of V. cholerae. The genome sequencing project of this organism is in progress, and the genes that are highly homologous to the essential genes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.6.1927-1930.1999
更新日期:1999-03-01 00:00:00
abstract::The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00511-19
更新日期:2019-11-20 00:00:00
abstract::Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture la...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.19.5668-5674.2001
更新日期:2001-10-01 00:00:00
abstract::Campylobacter jejuni commensally colonizes the cecum of birds. The RacR (reduced ability to colonize) response regulator was previously shown to be important in avian colonization. To explore the means by which RacR and its cognate sensor kinase RacS may modulate C. jejuni physiology and colonization, ΔracR and ΔracS ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06041-11
更新日期:2012-05-01 00:00:00
abstract::Cytotoxicity is an important virulence determinant in the pathogenesis of Vibrio vulnificus, and two cytotoxins, RTX (encoded by rtxA1) and cytolysin/hemolysin (encoded by vvhA), have been identified in this organism. We showed that the quorum-sensing regulator LuxO controlled the cytotoxicity of this organism: a Δlux...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01259-10
更新日期:2011-05-01 00:00:00
abstract::Mechanisms of Li+ stimulation of proline transport were studied in cells of Escherichia coli 7 and NR70, a mutant of strain 7 lacking adenosine triphosphatase (EC 3.6.1.3). An electrochemical potential difference of Li+ induced in an inward direction of energy-depleted cells caused a transient uptake of proline depend...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.560-564.1979
更新日期:1979-08-01 00:00:00
abstract::We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.24.8094-8101.1992
更新日期:1992-12-01 00:00:00
abstract::Mutants with altered forms of GltC, a positive LysR-type regulator of Bacillus subtilis glutamate synthase gene expression, were isolated. The mutant GltC proteins stimulated expression from the wild-type gltA promoter region 1.5- to 2.0-fold and from mutant promoter regions up to 80-fold. Moreover, expression of gltA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.19.5696-5700.1995
更新日期:1995-10-01 00:00:00
abstract:UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00422-15
更新日期:2015-08-31 00:00:00
abstract::Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00468-16
更新日期:2016-09-22 00:00:00
abstract::An RNA polymerase mutant with a single-base-pair change in the rpoC gene affects chromosome initiation control. The mutation, which is recessive, is a G to A transition leading to the substitution of aspartate for glycine at amino acid residue 1033 in the RNA polymerase beta' subunit. The chromosome copy number is inc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.16.5200-5206.1991
更新日期:1991-08-01 00:00:00
abstract::Lag is a temporary period of nonreplication seen in bacteria that are introduced to new media. Despite latency being described by Müller in 1895, only recently have we gained insights into the cellular processes characterizing lag phase. This review covers literature to date on the transcriptomic, proteomic, metabolom...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00697-18
更新日期:2019-03-13 00:00:00
abstract::Analyses of the Staphylococcus epidermidis multiresistance plasmids pSK697 and pSK818 have revealed them to be closely related to the trimethoprim resistance plasmid pSK639, also isolated from S. epidermidis. pSK697 and pSK818 were found to contain a cointegrated copy of a second plasmid related to the S. epidermidis ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.20.6070-6073.1996
更新日期:1996-10-01 00:00:00
abstract::We used Southern blotting to screen a variety of bacterial genes for homology to the kdp genes of Escherichia coli, genes that encode an ATP-driven K+ transport system. We found that most enterobacteria have sequences homologous to those of the three kdp structural genes and the kdpD regulatory gene. A number of dista...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.1192-1195.1989
更新日期:1989-02-01 00:00:00
abstract:UNLABELLED:CbrA is an atypical sensor kinase found in Pseudomonas. The autokinase domain is connected to a putative transporter of the sodium/solute symporter family (SSSF). CbrA functions together with its cognate response regulator, CbrB, and plays an important role in nutrient acquisition, including regulation of hu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00361-15
更新日期:2015-09-01 00:00:00
abstract::Pseudomonas aeruginosa in the lungs of cystic fibrosis (CF) patients is characterized by a series of genotypic and phenotypic changes that reflect the transition from acute to chronic infection. These include the overproduction of the exopolysaccharide alginate and the loss of complete lipopolysaccharide (LPS). LPS is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01999-12
更新日期:2013-04-01 00:00:00
abstract::The double-stranded DNA bacteriophage PRD1 uses an IncP plasmid-encoded conjugal transfer complex as a receptor. Plasmid functions in the PRD1 life cycle are restricted to phage adsorption and DNA entry. A single phage structural protein, P2, located at the fivefold capsid vertices, is responsible for PRD1 attachment ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.21.6689-6696.1999
更新日期:1999-11-01 00:00:00
abstract::The CpxA-CpxR two-component signal transduction pathway of Escherichia coli was studied in a mutant (pss-93) lacking phosphatidylethanolamine (PE). Several properties of this mutant are comparable to phenotypes of cpxA point mutants, indicating that this two-component pathway is activated in PE-deficient cells. In con...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.4.1029-1034.1997
更新日期:1997-02-01 00:00:00