Abstract:
:Campylobacter jejuni commensally colonizes the cecum of birds. The RacR (reduced ability to colonize) response regulator was previously shown to be important in avian colonization. To explore the means by which RacR and its cognate sensor kinase RacS may modulate C. jejuni physiology and colonization, ΔracR and ΔracS mutations were constructed in the invasive, virulent strain 81-176, and extensive phenotypic analyses were undertaken. Both the ΔracR and ΔracS mutants exhibited a ~100-fold defect in chick colonization despite no (ΔracS) or minimal (ΔracR) growth defects at 42 °C, the avian body temperature. Each mutant was defective for colony formation at 44°C and in the presence of 0.8% NaCl, both of which are stresses associated with the heat shock response. Promoter-reporter and real-time quantitative PCR (RT-qPCR) analyses revealed that RacR activates racRS and represses dnaJ. Although disregulation of several other heat shock genes was not observed at 38°C, the ΔracR and ΔracS mutants exhibited diminished upregulation of these genes upon a rapid temperature upshift. Furthermore, the ΔracR and ΔracS mutants displayed increased length heterogeneity during exponential growth, with a high proportion of filamented bacteria. Filamented bacteria had reduced swimming speed and were defective for invasion of Caco-2 epithelial cells. Soft-agar studies also revealed that the loss of racR or racS resulted in whole-population motility defects in viscous medium. These findings reveal new roles for RacRS in C. jejuni physiology, each of which is likely important during colonization of the avian host.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Apel D,Ellermeier J,Pryjma M,Dirita VJ,Gaynor ECdoi
10.1128/JB.06041-11subject
Has Abstractpub_date
2012-05-01 00:00:00pages
2342-54issue
9eissn
0021-9193issn
1098-5530pii
JB.06041-11journal_volume
194pub_type
杂志文章abstract::Aggregative amoebae of Dictyostelium discoideum, D. mucoroides, D. purpureum, and D. rosarium react chemotactically to cyclic AMP (cAMP). We measured the chemotactic activity of 14 cAMP analogs and found that these four species have a similar sensitivity to chemical modifications of cAMP; this suggests that the cAMP r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.99-105.1982
更新日期:1982-01-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.119.2.410-415.1974
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JB.120.3.999-1003.1974
更新日期:1974-12-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.413-420.1977
更新日期:1977-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.3.955-959.1971
更新日期:1971-06-01 00:00:00
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更新日期:1972-01-01 00:00:00
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更新日期:1984-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1989-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-03-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.180.16.4287-4290.1998
更新日期:1998-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.2.640-645.1970
更新日期:1970-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.10.3212-3219.1992
更新日期:1992-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.145-160.1973
更新日期:1973-01-01 00:00:00