Abstract:
:DNA involved in the mobilization of broad-host-range plasmid R1162 was localized to a region of 2.7 kilobases within coordinates 3.4 to 6.1 kilobases on the R1162 map. By examining the transfer properties of plasmids containing cloned fragments of DNA from within this region, we showed that at least four trans-active products and a cis-active site (oriT) were involved in mobilization. A cloned DNA fragment of 155 base pairs was capable of providing full oriT activity. This fragment was located within 600 base pairs of DNA containing the origin of replication of R1162, and its nucleotide sequence and that of neighboring DNA were determined. Activation of oriT required R1162-encoded, trans-acting products. Deletions which resulted in the loss of one or more of these had a variable effect on transfer efficiency and indicated the presence of both essential and nonessential Mob products. Regions encoding these products flanked oriT and in one case appeared to overlap a gene essential for plasmid replication. The implications of these findings with respect to the broad host range of R1162 are discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Brasch MA,Meyer RJdoi
10.1128/jb.167.2.703-710.1986subject
Has Abstractpub_date
1986-08-01 00:00:00pages
703-10issue
2eissn
0021-9193issn
1098-5530journal_volume
167pub_type
杂志文章abstract::Acid hydrolysates of 140 strains, representing 11 species of the genus Bacteroides, were analyzed by capillary gas-liquid chromatography for total cellular fatty acid. All samples contained components which appeared to be hydroxy fatty acid. The relative amount and chain length distribution of the hydroxy fatty acids,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.582-587.1980
更新日期:1980-08-01 00:00:00
abstract::Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05989-11
更新日期:2012-03-01 00:00:00
abstract::Liquid holding recovery (LHR) in ultraviolet-irradiated Bacteroides fragilis cells occurred under aerobic conditions but was inhibited by anaerobic conditions. The increase in survival after aerobic LHR resulted in an increase in the shoulder regions of the ultraviolet survival curves. Maximum LHR was obtained after h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.1.1-7.1981
更新日期:1981-01-01 00:00:00
abstract::Spo0A is a DNA binding protein in Bacillus subtilis required for the activation of spoIIG and other promoters at the onset of endospore formation. Activation of some of these promoters may involve interaction of Spo0A and the sigmaA subunit of RNA polymerase. Previous studies identified two single-amino-acid substitut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.14.3578-3583.1998
更新日期:1998-07-01 00:00:00
abstract::We have investigated the mechanism of osmotic stress adaptation (osmoregulation) in Agrobacterium tumefaciens biotype I (salt-tolerant) and biotype II (salt-sensitive) strains. Using natural-abundance 13C nuclear magnetic resonance spectroscopy, we identified all organic solutes that accumulated to significant levels ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.12.6849-6855.1990
更新日期:1990-12-01 00:00:00
abstract::The highly purified lipoprotein of the outer membrane of Escherichia coli forms paracrystals. The ultrastructures of these paracrystals were examined by electron microscopy. The needle-shaped paracrystals show several different band patterns, depending on conditions of paracrystallization. Models are presented to expl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.564-571.1976
更新日期:1976-07-01 00:00:00
abstract::An in vitro system of cell envelopes from Salmonella typhimurium with functional flagella was used to determine the minimal requirements for flagellar rotation. Rotation in the absence of cytoplasmic constituents could be driven either by respiration or by an artificially imposed chemical gradient of protons. No speci...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.1208-1210.1984
更新日期:1984-06-01 00:00:00
abstract::We used Southern blotting to screen a variety of bacterial genes for homology to the kdp genes of Escherichia coli, genes that encode an ATP-driven K+ transport system. We found that most enterobacteria have sequences homologous to those of the three kdp structural genes and the kdpD regulatory gene. A number of dista...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.2.1192-1195.1989
更新日期:1989-02-01 00:00:00
abstract::Protocatechuate 3,4-dioxygenase is a member of a family of bacterial enzymes that cleave the aromatic rings of their substrates between two adjacent hydroxyl groups, a key reaction in microbial metabolism of varied environmental chemicals. In an appropriate genetic background, it is possible to select for Acinetobacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.20.6478-6487.1999
更新日期:1999-10-01 00:00:00
abstract::The Escherichia coli flaA gene product (also called cheC) plays a crucial role in switching flagellar rotational direction during chemotactic responses. Wild-type and mutant alleles have been cloned onto plasmid vectors, and the gene product has been identified as a 37,000-dalton protein. The flaA product appeared as ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.398-405.1985
更新日期:1985-04-01 00:00:00
abstract::Coliphage TuIa, which uses the OmpF protein as a receptor, can adapt by mutation to using instead the OmpC or LamB protein, or both. Most of the phage mutants retained the ability to use the OmpF protein, when present, but one class of mutants lost this ability and could only use the OmpC protein. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.3.1182-1185.1980
更新日期:1980-12-01 00:00:00
abstract::The fim operon of Salmonella enterica serovar Typhimurium encodes type 1 fimbriae. The expression of fim is controlled in response to environmental signals through a complex regulatory cascade involving the proteins FimW, FimY, and FimZ and a genetic locus, fimU, that encodes a rare arginine tRNA. We discovered that a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01388-07
更新日期:2008-01-01 00:00:00
abstract::Studies on the active product of pepsin digestion of rabbit immunoglobulin G (IgG), F(ab')(2) produced from antiserum against Neurospora glutamate dehydrogenase, showed it to behave in the same way, in both antienzyme and precipitation experiments, as homologous IgG. It is proposed that the inhibition of glutamate deh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.958-962.1967
更新日期:1967-10-01 00:00:00
abstract::Synthesis of the autoinducer signal involved in the cell density-dependent activation of Vibrio fischeri luminescence is directed by luxI. The autoinducer is N-(3-oxohexanoyl)homoserine lactone, and little is known about its synthesis. We have measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.17.5291-5294.1996
更新日期:1996-09-01 00:00:00
abstract::The flagella of Methanococcus voltae were isolated by using three procedures. Initially, cells were sheared to release the filaments, which were purified by differential centrifugation and banding in KBr gradients. Flagella were also prepared by solubilization of cells with 1% (vol/vol) Triton X-100 and purified as de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1752-1758.1988
更新日期:1988-04-01 00:00:00
abstract::The replication order of markers was studied in Bacillus subtilis strains bearing the trpE26 mutation by the use of the density transfer technique. An important difference in this order was observed in comparison with that of strain 168 T-. All markers tested of a chromosome segment extending from trpD to ilvA replica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.886-898.1975
更新日期:1975-06-01 00:00:00
abstract::C(1), a lytic bacteriophage infecting group C streptococci, is one of the earliest-isolated phages, and the method of bacterial classification known as phage typing was defined by using this bacteriophage. We present for the first time a detailed analysis of this phage by use of electron microscopy, protein profiling,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.11.3325-3332.2003
更新日期:2003-06-01 00:00:00
abstract::Ikeda, Y. (University of Tokyo, Tokyo, Japan) and T. Iijima. Heterocaryosis-like phenomenon in Bacillus subtilis. J. Bacteriol. 83:1025-1028. 1962.-When a mixture of methionine-dependent, histidine-dependent, streptomycin-resistant cells and tryptophan-dependent, streptomycin-sensitive cells of Bacillus subtilis K is ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.5.1025-1028.1962
更新日期:1962-05-01 00:00:00
abstract::Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found to trigger biofilm dispersal in the opportunistic pathogen Pseudomonas aeruginosa at low, nontoxic concentrations ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00975-09
更新日期:2009-12-01 00:00:00
abstract::Plasmid pUB110 was previously used as a vector to clone fragments of deoxyribonucleic acid that complement the trpC2 mutation in Bacillus subtilis from endonuclease EcoRI digested B. licheniformis, B. pumilus, and B. subtilis cellular deoxyribonucleic acid. Each of several such trp plasmids was subsequently shown to c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.1001-1006.1979
更新日期:1979-09-01 00:00:00
abstract::In a search for genes that produce hypersensitivity to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used. One of the mutant strains obtained was sensitive to Cd2+ and Zn2+. Sequence analysis showed that the TnphoA insertion was located in the dsbA gene coding for a periplasmic prote...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.8.2769-2771.1997
更新日期:1997-04-01 00:00:00
abstract::The Firmicutes Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes convert thiosulfate, forming sulfur globules inside and outside cells. X-ray absorption near-edge structure analysis revealed that the sulfur consisted mainly of sulfur chains with organic end groups similar to sulfur formed ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00782-07
更新日期:2007-10-01 00:00:00
abstract::The growth cycle of Rickettsia quintana was studied for the first time in liquid culture. Growth of the microorganism in a transparent broth medium was made possible by the finding that fetal calf serum (FCS), but not calf serum (CS), satisfied the requirement of R. quintana (Fuller strain) for red blood cell lysate. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.1.184-190.1970
更新日期:1970-07-01 00:00:00
abstract::A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.6.2521-2526.1988
更新日期:1988-06-01 00:00:00
abstract::Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, a quinolone related to nalidixic acid. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.5.2385-2387.1988
更新日期:1988-05-01 00:00:00
abstract::Wallace, W. R. (Louisiana State University, Baton Rouge), and G. T. Dimopoullos. Catalase activity in Anaplasma marginale. J. Bacteriol. 91:309-311. 1965.-Extracts of erythrocytes infected with Anaplasma marginale were found to contain more catalase activity than normal erythrocytic preparations. The increase in catal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.309-311.1965
更新日期:1965-08-01 00:00:00
abstract::Cores are large, rod-shaped structures that have been found almost exclusively in group D streptococci, measure 0.1 to 0.16 mum in diameter, and extend the width or length of cells. This study has shown that cores are produced in the cells at a reproducible point in early stationary growth after extensive mesosomal fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.445-456.1977
更新日期:1977-01-01 00:00:00
abstract::PsaA, the subunit of the fimbria originally referred to as the "pH 6 antigen," is required for full virulence of Yersinia pestis during bubonic plague. The expression of psaA is dependent upon specific environmental signals, and while the signals (high temperature and acidic pH) are defined, the mechanisms underlying ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00217-19
更新日期:2019-07-24 00:00:00
abstract::Bacterial cell surface components can be important determinants of virulence. At least three gene clusters important for extracellular polysaccharide (EPS) biosynthesis have been previously identified in the plant pathogen Pseudomonas solanacearum. We have found that one of these gene clusters, named ops, is also requ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.24.7841-7847.1991
更新日期:1991-12-01 00:00:00
abstract::Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05851-11
更新日期:2011-10-01 00:00:00