Abstract:
:PsaA, the subunit of the fimbria originally referred to as the "pH 6 antigen," is required for full virulence of Yersinia pestis during bubonic plague. The expression of psaA is dependent upon specific environmental signals, and while the signals (high temperature and acidic pH) are defined, the mechanisms underlying this regulation remain unclear. In the closely related species Yersinia pseudotuberculosis, psaA transcription requires two regulatory genes, psaE and psaF, and it is speculated that posttranscriptional regulation of PsaE and/or PsaF contributes to the regulation of psaA transcription. Few studies have examined the regulation of psaA expression in Y. pestis, and prior to this work, the roles of psaE and psaF in Y. pestis had not been defined. The data presented here show that both psaE and psaF are required for psaA transcription in Y. pestis and that the impact of temperature and pH is mediated through discrete posttranscriptional effects on PsaE and PsaF. By generating antibodies that recognize endogenous PsaE and PsaF, we determined that the levels of both proteins are impacted by temperature and pH. High temperature is required for psaE and psaF translation via discrete mechanisms mediated by the mRNA 5' untranslated region (UTR) upstream of each gene. Additionally, levels of PsaE and PsaF are impacted by pH. We show that PsaF enhances the stability of PsaE, and thus, both PsaE and PsaF are required for psaA transcription. Our data indicate that the environmental signals (temperature and pH) impact the expression of psaA by affecting the translation of psaE and psaF and the stability of PsaE and PsaF.IMPORTANCEY. pestis is a Gram-negative bacterial pathogen that causes bubonic plague. As a vector-borne pathogen, Y. pestis fluctuates between an arthropod vector (flea) and mammalian host. As such, Y. pestis must recognize environmental signals encountered within each host environment and respond by appropriately regulating gene expression. PsaA is a key Y. pestis mammalian virulence determinant that forms fimbriae. Our work provides evidence that Y. pestis utilizes multiple posttranscriptional mechanisms to regulate the levels of two PsaA regulatory proteins in response to both temperature and pH. This study offers insight into mechanisms that bacteria utilize to sense environmental cues and regulate the expression of determinants required for mammalian disease.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Quinn JD,Weening EH,Miner TA,Miller VLdoi
10.1128/JB.00217-19subject
Has Abstractpub_date
2019-07-24 00:00:00issue
16eissn
0021-9193issn
1098-5530pii
JB.00217-19journal_volume
201pub_type
杂志文章abstract::The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth r...
journal_title:Journal of bacteriology
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doi:10.1128/JB.158.1.296-299.1984
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doi:10.1128/jb.177.24.7131-7140.1995
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更新日期:1969-01-01 00:00:00
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更新日期:1983-04-01 00:00:00
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doi:10.1128/jb.172.4.2113-2123.1990
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doi:10.1128/JB.101.1.218-231.1970
更新日期:1970-01-01 00:00:00
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更新日期:1992-04-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1997-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
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更新日期:1999-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.21.6352-6356.1996
更新日期:1996-11-01 00:00:00
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