Genetic analysis of thymidine-resistant and low-thymine-requiring mutants of Escherichia coli K-12 induced by bacteriophage Mu-1.

abstract：:SaPIbov2 is a member of the SaPI family of staphylococcal pathogenicity islands and is very closely related to SaPIbov1. Typically, certain temperate phages can induce excision and replication of one or more of these islands and can package them into special small phage-like particles commensurate with their genome si...

journal_title：Journal of bacteriology

authors： Maiques E,Ubeda C,Tormo MA,Ferrer MD,Lasa I,Novick RP,Penadés JR

更新日期：2007-08-01 00:00:00

abstract：:By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order...

journal_title：Journal of bacteriology

authors： Tucker SD,Gopalakrishnan AS,Bollinger R,Dowhan W,Murgola EJ

更新日期：1982-11-01 00:00:00

abstract：UNLABELLED:Many bacteria utilize actin-like proteins to direct peptidoglycan (PG) synthesis. MreB and MreB-like proteins are thought to act as scaffolds, guiding the localization and activity of key PG-synthesizing proteins during cell elongation. Despite their critical role in viability and cell shape maintenance, ver...

journal_title：Journal of bacteriology

authors： Duan Y,Sperber AM,Herman JK

更新日期：2016-07-13 00:00:00

abstract：:It has been clear for over sixty years that the principal method whereby cells replicate and segregate their DNA is semiconservative. It is much less clear why it should be like this rather than, say, conservative. Recently, evidence has accumulated that supports the hypothesis that one of the functions of the cell cy...

journal_title：Journal of bacteriology

authors： Norris V

更新日期：2019-05-22 00:00:00

abstract：:Heterocysts isolated from Anabaena sp. strain 7120 with lysozyme plus sonication were permeabilized with the cationic detergent cetyltrimethylammonium bromide, and they then exhibited comparable acetylene reduction activity in the light and dark with an ATP-regenerating system plus dithionite. The detergent diminished...

journal_title：Journal of bacteriology

authors： Privalle LS,Burris RH

更新日期：1983-08-01 00:00:00

abstract：:Natural isolates of pathogenic bacteria can exhibit a broad range of phenotypic traits. To investigate the molecular mechanisms contributing to such phenotypic variability, we compared the genomes, transcriptomes, and proteomes of two natural isolates of the gram-negative bacterium Burkholderia pseudomallei, the causa...

journal_title：Journal of bacteriology

authors： Ou K,Ong C,Koh SY,Rodrigues F,Sim SH,Wong D,Ooi CH,Ng KC,Jikuya H,Yau CC,Soon SY,Kesuma D,Lee MA,Tan P

更新日期：2005-06-01 00:00:00

abstract：:MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...

journal_title：Journal of bacteriology

authors： van den Brink-van der Laan E,Boots JW,Spelbrink RE,Kool GM,Breukink E,Killian JA,de Kruijff B

更新日期：2003-07-01 00:00:00

abstract：:Genetic exchange mechanisms, to our knowledge, have not been reported for Legionella pneumophila, and consequently, studies on the genetic organization of L. pneumophila have not appeared in the literature. Here, we describe gene transfer mediated by broad host range conjugative plasmids in Legionella spp. Escherichia...

journal_title：Journal of bacteriology

authors： Dreyfus LA,Iglewski BH

更新日期：1985-01-01 00:00:00

abstract：:Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Ins...

journal_title：Journal of bacteriology

authors： Maynard A,Butler NL,Ito T,da Silva AJ,Murai M,Chen T,Koffas MAG,Miyoshi H,Barquera B

更新日期：2019-05-08 00:00:00

abstract：:The two-component regulatory system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription. In this article, we describe a new OmpR-binding site that is lo...

journal_title：Journal of bacteriology

authors： Huang KJ,Schieberl JL,Igo MM

更新日期：1994-03-01 00:00:00

abstract：:Saccharomyces cerevisiae was grown in a continuous culture at a single dilution rate with input ammonia concentrations whose effects ranged from nitrogen limitation to nitrogen excess and glucose limitation. The rate of ammonia assimilation (in millimoles per gram of cells per hour) was approximately constant. Increas...

journal_title：Journal of bacteriology

authors： ter Schure EG,Silljé HH,Verkleij AJ,Boonstra J,Verrips CT

更新日期：1995-11-01 00:00:00

abstract：:The heme prosthetic group of heme proteins contains iron, which can be a limiting nutrient. Here, we show that cytochrome c1 protein from Bradyrhizobium japonicum was strongly affected by the iron status, with low expression in cells grown under iron limitation. This control was not affected in mutants encoding the ir...

journal_title：Journal of bacteriology

authors： Gao T,O'Brian MR

更新日期：2005-08-01 00:00:00

abstract：:Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA po...

journal_title：Journal of bacteriology

authors： Sebastian J,Bhargava MM,Halvorson HO

更新日期：1973-04-01 00:00:00

abstract：:Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...

journal_title：Journal of bacteriology

authors： Madigan MT,Brock TD

更新日期：1975-05-01 00:00:00

abstract：:The global regulatory protein Crc is involved in the repression of several catabolic pathways for sugars, hydrocarbons, and nitrogenated and aromatic compounds in Pseudomonas putida and Pseudomonas aeruginosa when other preferred carbon sources are present in the culture medium (catabolite repression), therefore modul...

journal_title：Journal of bacteriology

authors： Ruiz-Manzano A,Yuste L,Rojo F

更新日期：2005-06-01 00:00:00

abstract：:This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identica...

journal_title：Journal of bacteriology

authors： Vakharia H,German GJ,Misra R

更新日期：2001-12-01 00:00:00

abstract：:Crude cell extracts of Bacillus subtilis 168T exhibit enzyme activity capable of releasing free uracil from phage PBS1 deoxyribonucleic acid (DNA) in the presence of ethylenediaminetetraacetate. By measuring the enzyme activity in 300 clones that emanated from mutagenized cells, we obtained a mutant strain that did no...

journal_title：Journal of bacteriology

authors：

更新日期：1977-08-01 00:00:00

abstract：:Apparent pK values were determined for a series of commercially available carboxylic acids in a search for a buffer suitable for bacterial studies at pH 7 in media free from nitrogen, sulfur, and phosphorus. Of the compounds possessing pK' values near pH 7,3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid (EMTA) was c...

journal_title：Journal of bacteriology

authors： Mallette MF

更新日期：1967-08-01 00:00:00

abstract：:An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

journal_title：Journal of bacteriology

authors： Woldringh CL,van Iterson W

更新日期：1972-09-01 00:00:00

abstract：:The host range of an octopine Ti plasmid is limited to Rhizobiaceae. This has been extended also to Escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid R772. Its structure was studied by constructing a physical map of R772 and of the R772::pTiB6 cointegrate. An insertion sequence pres...

journal_title：Journal of bacteriology

authors： Hille J,van Kan J,Klasen I,Schilperoort R

更新日期：1983-05-01 00:00:00

abstract：:Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...

journal_title：Journal of bacteriology

authors： Brunskill EW,Bayles KW

更新日期：1996-02-01 00:00:00

abstract：:Growth of Schizosaccharomyces pombe in batch cultures in a commercial defined medium was found to be normal until the last generation before the stationary phase. Approximately one-half the progeny of the last division lysed. Lysis occurred at breaks in the cell wall at cell-plates whose fission was eccentric. Normal ...

journal_title：Journal of bacteriology

authors： Johnson BF

更新日期：1967-07-01 00:00:00

abstract：:Members of all four families of methanogenic bacteria were analyzed for polyamine concentrations. High-performance liquid chromatography analysis of dansylated cell extracts revealed typical polyamine patterns for each family. Members of Methanobacteriaceae (family I) were characterized by very low polyamine concentra...

journal_title：Journal of bacteriology

authors： Scherer P,Kneifel H

更新日期：1983-06-01 00:00:00

abstract：:Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed rev...

journal_title：Journal of bacteriology

authors： Thiele I,Vo TD,Price ND,Palsson BØ

更新日期：2005-08-01 00:00:00

abstract：:Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

journal_title：Journal of bacteriology

authors： Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

更新日期：1990-09-01 00:00:00

abstract：:The expression of R-factor-mediated resistance to tetracycline has been compared in Proteus mirabilis and Escherichia coli. Resistance to a range of concentrations of tetracycline was significantly lower in P. mirabilis than in E. coli in both induced and repressed states. Indirect evidence showed that conditions whic...

journal_title：Journal of bacteriology

authors： Franklin TJ,Rownd R

更新日期：1973-07-01 00:00:00

abstract：:Three gene libraries of Bordetella avium 197 DNA were prepared in Escherichia coli LE392 by using the cosmid vectors pCP13 and pYA2329, a derivative of pCP13 specifying spectinomycin resistance. The cosmid libraries were screened with convalescent-phase anti-B. avium turkey sera and polyclonal rabbit antisera against ...

journal_title：Journal of bacteriology

authors： Gentry-Weeks CR,Hultsch AL,Kelly SM,Keith JM,Curtiss R 3rd

更新日期：1992-12-01 00:00:00

abstract：:Cultures of a stable L-phase variant of Streptococcus faecium F24 produced and retained peptidoglycan precursors intracellularly over the entire growth cycle in a chemically defined medium. The identity of the most abundant precursor, UDP N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine (UDP-MurNAc-penta...

journal_title：Journal of bacteriology

authors： Gregory WW,Gooder H

更新日期：1978-09-01 00:00:00

abstract：:Since relatively few quantitative immunological studies had been conducted with invertebrates, this study was designed to learn more about the immune potential of the oyster, a molluscan representative. Experiments measured the primary and secondary clearance rates of T2 coliphage in the oyster. The fate of phage inje...

journal_title：Journal of bacteriology

authors： Acton RT,Evans EE

更新日期：1968-04-01 00:00:00

abstract：:Selenium has been shown to be present as a labile cofactor in a small class of molybdenum hydroxylase enzymes in several species of clostridia that specialize in the fermentation of purines and pyrimidines. This labile cofactor is poorly understood, yet recent bioinformatic studies have suggested that Enterococcus fae...

journal_title：Journal of bacteriology

authors： Srivastava M,Mallard C,Barke T,Hancock LE,Self WT

更新日期：2011-04-01 00:00:00