Growth of the fission yeast, Schizosaccharomyces pombe, with late, eccentric, lytic fission in an unbalanced medium.

Abstract:

:Growth of Schizosaccharomyces pombe in batch cultures in a commercial defined medium was found to be normal until the last generation before the stationary phase. Approximately one-half the progeny of the last division lysed. Lysis occurred at breaks in the cell wall at cell-plates whose fission was eccentric. Normal culture cycle patterns were obtained when the medium was balanced by addition of either 0.20 mg of l-asparagine per ml or 5.0 mg of KH(2)PO(4) per ml, or both. The lytic effect of growth in the unbalanced medium was compared with similar effects of growth in 2-deoxyglucose. These studies with 2-deoxyglucose in the balanced medium confirmed earlier cytological observations (lysis at all growing points), but showed that the similarities were superficial.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Johnson BF

doi

10.1128/JB.94.1.192-195.1967

subject

Has Abstract

pub_date

1967-07-01 00:00:00

pages

192-5

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

94

pub_type

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