Abstract:
:Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the stress-responsive RNA polymerase sigma factor. The mutation appeared to cause RsbU to be hyperactive, because the mutants were more resistant than the parent strain to ethanol stress. In support of this hypothesis, pseudorevertants that regained wild-type levels of sporulation at high temperature had secondary mutations that prevented expression of the mutant rsbU gene. The properties of these RsbU mutants support the idea that activation of SigB diminishes the bacterium's ability to sporulate.IMPORTANCE Most bacterial species encode multiple RNA polymerase promoter recognition subunits (sigma factors). Each sigma factor directs RNA polymerase to different sets of genes; each gene set typically encodes proteins important for responses to specific environmental conditions, such as changes in temperature, salt concentration, and nutrient availability. A selection for mutants of Bacillus subtilis that are temperature sensitive for RNA synthesis during sporulation unexpectedly yielded strains with a point mutation in rsbU, a gene that encodes a protein that normally activates sigma factor B (SigB) under conditions of salt stress. The mutation appears to cause RsbU, and therefore SigB, to be active inappropriately, thereby inhibiting, directly or indirectly, the ability of the cells to transcribe sporulation genes.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Rothstein DM,Lazinski D,Osburne MS,Sonenshein ALdoi
10.1128/JB.00212-17subject
Has Abstractpub_date
2017-06-27 00:00:00issue
14eissn
0021-9193issn
1098-5530pii
JB.00212-17journal_volume
199pub_type
杂志文章abstract::Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.279-287.1984
更新日期:1984-10-01 00:00:00
abstract::Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.6205-6209.1999
更新日期:1999-10-01 00:00:00
abstract::Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.159-168.1983
更新日期:1983-07-01 00:00:00
abstract::The cell wall degradation products released from Escherichia coli during autolysis triggered by cephaloridine or trichloroacetic acid were isolated and characterized. Murein was selectively lost from the disaccharide tetrapeptides and the bisdisaccharide tetrapeptide components. Two major autolytic products accounted ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.759-765.1986
更新日期:1986-09-01 00:00:00
abstract::Weaver, Judith R. (Iowa State University, Ames), and P. A. Pattee. Inducible resistance to erythromycin in Staphylococcus aureus. J. Bacteriol. 88:574-580. 1964.-The dissociated resistance of Staphylococcus aureus to erythromycin was examined and was found to possess the characteristics of an inducible enzyme. The ind...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.574-580.1964
更新日期:1964-09-01 00:00:00
abstract::A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6864-6875.2004
更新日期:2004-10-01 00:00:00
abstract::Shewanella putrefaciens strain 200 respires a wide range of compounds as terminal electron acceptor. The respiratory versatility of Shewanella is attributed in part to a set of c-type cytochromes with widely varying midpoint redox potentials (E'(0)). A point mutant of S. putrefaciens, originally designated Urr14 and h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01249-06
更新日期:2007-02-01 00:00:00
abstract::A chemotaxis system for Pseudomonas aeruginosa was defined by using the method of Adler. Cells were attracted to compounds in the order ammonium chloride greater than amino acids greater than organic acids. Two sugars were assayed and elicited no response. Comparisons with other model systems are discussed. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.427-429.1978
更新日期:1978-01-01 00:00:00
abstract::The mutB gene of Salmonella typhimurium is involved in a methylation-independent repair pathway specific for A/G or A/C mismatches and is the homolog of the Escherichia coli mutY gene. The mutB gene of S. typhimurium was cloned and sequenced. The isolated mutB clone reduced the mutation rate of the mutB mutant to wild...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.2.541-543.1993
更新日期:1993-01-01 00:00:00
abstract::The emerging coverage of diverse habitats by metagenomic shotgun data opens new avenues of discovering functional novelty using computational tools. Here, we apply three different concepts for predicting novel functions within light-mediated microbial pathways in five diverse environments. Using phylogenetic approache...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.01084-08
更新日期:2009-01-01 00:00:00
abstract::The pattern of transcription of the rplKAJLrpoBC gene cluster of Escherichia coli appears to be complex. At least four different promoters and a transcriptional attenuator have been identified. To compare the relative effect of each of the putative promoters and the attenuator on transcription of these genes, we fused...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.279-285.1984
更新日期:1984-04-01 00:00:00
abstract::The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated wi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.29-35.1982
更新日期:1982-07-01 00:00:00
abstract::The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.3.821-824.2000
更新日期:2000-02-01 00:00:00
abstract::Cells of the cyanobacterium Anabaena variabilis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1143-1147.1988
更新日期:1988-03-01 00:00:00
abstract::We report a phenomenon similar to catabolite repression in Rhizobium meliloti. Succinate, which allows the highest observed rate of growth of R. meliloti, caused an immediate reduction of beta-galactosidase activity when added to cells growing in lactose. A Lac- mutant was unaltered in nodulation and nitrogen fixation...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.1197-1200.1978
更新日期:1978-12-01 00:00:00
abstract::The diphtheria toxin repressor (DtxR) of Corynebacterium diphtheriae uses Fe(2+) as a corepressor. Holo-DtxR inhibits transcription from the iron-regulated promoters (IRPs) designated IRP1 through IRP5 as well as from the promoters for the tox and hmuO genes. DtxR binds to 19-bp operators with the consensus sequence 5...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.2.432-438.2000
更新日期:2000-01-01 00:00:00
abstract::Shewanella oneidensis MR-1 is a facultatively anaerobic bacterium capable of using soluble and insoluble forms of manganese [Mn(III/IV)] and iron [Fe(III)] as terminal electron acceptors during anaerobic respiration. To assess the structural association of two outer membrane-associated c-type decaheme cytochromes (i.e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01966-05
更新日期:2006-07-01 00:00:00
abstract::Most motile bacteria are able to bias their movement towards more favorable environments or to escape from obnoxious substances by a process called chemotaxis. Chemotaxis depends on a chemosensory system that is able to sense specific environmental signals and generate a behavioral response. Typically, the signal is t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00793-17
更新日期:2018-03-12 00:00:00
abstract::After treatment with N-methyl-N'-nitro-N-nitrosoguanidine, 133 independent mutants of a haploid strain of Escherichia coli able to use phenyl-beta-galactoside as a carbon source were obtained. The galactoside was specific in selecting for mutants with increases in their uninduced levels of beta-galactosidase. Virtuall...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.500-507.1974
更新日期:1974-08-01 00:00:00
abstract::Synchrony in phototrophic cultures of Rhodospirillum rubrum was induced by stationary-phase cycling or by alterations in light intensity. Intracytoplasmic chromatophore membranes were prepared by differential centrifugation. Analysis of the composition of chromatophores obtained from cells at different times indicated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.818-823.1986
更新日期:1986-06-01 00:00:00
abstract::Accumulation of abnormal proteins in cells of bacteria or eukaryotes can induce synthesis of a set of heat shock proteins. We examined such induction following addition of azetidine (a proline analog) or synthesis of a heterologous protein (human prourokinase) in Escherichia coli. Synthesis of heat shock proteins unde...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5648-5653.1994
更新日期:1994-09-01 00:00:00
abstract::A nonpathogenic mutant of Erwinia carotovora obtained by Mu d1 mutagenesis was defective in the ability to utilize several carbon sources. The basis of the mutation was analyzed biochemically and shown to be a defect in the ability to form UDP glucose-pyrophosphorylase. The nonpathogenic phenotype of the mutant was ca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.473-476.1985
更新日期:1985-10-01 00:00:00
abstract::The general stress response in Alphaproteobacteria was recently described to depend on the alternative sigma factor σ(EcfG), whose activity is regulated by its anti-sigma factor NepR. The response regulator PhyR, in turn, regulates NepR activity in a partner-switching mechanism according to which phosphorylation of Ph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06006-11
更新日期:2011-12-01 00:00:00
abstract::The cell walls of selected bacteria were studied by X-ray diffraction analysis to determine and characterize crystalline components. The walls were isolated by mechanical disruption and purified by enzymatic and washing procedures. The X-ray diffraction lines which appeared from the gram-positive cell walls were shown...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.122-124.1967
更新日期:1967-01-01 00:00:00
abstract::A fusion between lacZ and ftsZ of Escherichia coli was constructed to obtain a beta-galactosidase-FtsZ fusion protein. This fusion protein was used to raise antibodies against cell division protein FtsZ. Six monoclonal antibodies were obtained, and they reacted with FtsZ from cytoplasm and membrane fractions. The epit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.1886-1893.1994
更新日期:1994-04-01 00:00:00
abstract::The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.3.1096-1100.2001
更新日期:2001-02-01 00:00:00
abstract::Klebsiella pneumoniae LZ is a bacterium isolated from soil which can produce 1,3-propanediol from glycerol. Here we present a 5,431,750-bp assembly of its genome sequence. We annotated 9 coding sequences (CDSs) responsible for glycerol fermentation to 1,3-propanediol, 19 CDSs encoding glycerol utilization, and 134 CDS...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00864-12
更新日期:2012-08-01 00:00:00
abstract::Allantoate uptake appears to be mediated by an energy-dependent active transport system with an apparent Michaelis constant of about 50 microM. Cells were able to accumulate allantoate to greater than 3,000 times the extracellular concentration. The rate of accumulation was maximum at pH 5.7 to 5.8. The energy source ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.971-979.1979
更新日期:1979-12-01 00:00:00
abstract::Izaki, Kazuo (University of Tokyo, Tokyo, Japan), Kan Kiuchi, and Kei Arima. Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bacteriol. 91:628-633. 1966.-A decrease in the uptake of tetracycline occurred concurrently with a rise in the level of resistanc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.628-633.1966
更新日期:1966-02-01 00:00:00
abstract::Guanine is transported into germinated conidia of Neurospora crassa by the general purine base transport system. Guanine uptake is inhibited by adenine and hypoxanthine but not xanthine. Guanine phosphoribosyltransferase (GPRTase) activity was demonstrated in cell extracts of wild-type germinated conidia. The Km for g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.941-947.1982
更新日期:1982-03-01 00:00:00