The Bacillus subtilis 168 alkaline phosphatase III gene: impact of a phoAIII mutation on total alkaline phosphatase synthesis.

Abstract:

:The first alkaline phosphatase (APase) structural gene mutant of Bacillus subtilis 168 was constructed by using a clone identified by hybridization to a synthetic degenerative oligonucleotide. The design of the probe was based on the first 29 amino acids of the sequenced mature APase III protein, which had been isolated from the secreted fraction of vegetative, phosphate-starved cells. DNA sequencing of the clone revealed the first 80 amino acids of the APase III protein, including a typical procaryotic signal sequence of 32 amino acids preceding the start of the mature protein. The 29 amino acids encoded by the predicted open reading frame immediately following the signal sequence are identical to the first 29 amino acids of the sequenced mature protein. This region shows 80% identity to strand A of the beta sheet that is very well conserved in Escherichia coli and mammalian APases. A phoAIII structural mutant was constructed by insertional mutagenesis with a fragment internal to the coding region. The effects of this mutation on APase production in B. subtilis 168 were analyzed under both phosphate starvation and sporulation conditions. The mutation in APase III reduced the total vegetative APase specific activity by approximately 40% and sporulation APase specific activity by approximately 45%. An APase protein was isolated from sporulating cells at stage III and was identified as APase III by protein sequencing of the amino terminus and by its absence in the phoAIII mutant. The APase III gene has been mapped to approximately 50 degrees on the B. subtilis chromosome.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bookstein C,Edwards CW,Kapp NV,Hulett FM

doi

10.1128/jb.172.7.3730-3737.1990

subject

Has Abstract

pub_date

1990-07-01 00:00:00

pages

3730-7

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

杂志文章
  • Identification of a phosphoenolpyruvate:fructose 1-phosphotransferase system in Azospirillum brasilense.

    abstract::An inducible phosphoenolpyruvate:fructose phosphotransferase system has been detected in Azospirillum brasilense, which requires a minimum of two components of the crude extracts for activity: (i) a soluble fraction (enzyme I) and (ii) a membrane fraction (enzyme II). The uninduced cells neither show any uptake of fru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.3.1204-1206.1984

    authors: Gupta KD,Ghosh S

    更新日期:1984-12-01 00:00:00

  • PENTOSE UTILIZATION BY PEDIOCOCCUS PENTOSACEUS.

    abstract::Dobrogosz, Walter J. (University of Illinois, Urbana) and Ralph D. DeMoss. Pentose utilization by Pediococcus pentosaceus. J. Bacteriol. 85:1356-1364. 1963.-Data are presented which indicate that pentoses are metabolized by Pediococcus pentosaceus through the use of an inducible phosphoketolase pathway. The utilizatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.6.1356-1364.1963

    authors: DOBROGOSZ WJ,DEMOSS RD

    更新日期:1963-06-01 00:00:00

  • Genome sequence of Rhizobium grahamii CCGE502, a broad-host-range symbiont with low nodulation competitiveness in Phaseolus vulgaris.

    abstract::Here we present the genome sequence of Rhizobium grahamii CCGE502. R. grahamii groups with other newly described broad-host-range species, which are not very efficient Phaseolus vulgaris symbionts, with a wide geographic distribution and which constitutes a novel Rhizobium clade. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01785-12

    authors: Althabegoiti MJ,Lozano L,Torres-Tejerizo G,Ormeño-Orrillo E,Rogel MA,González V,Martínez-Romero E

    更新日期:2012-12-01 00:00:00

  • Glycine betaine and potassium ion are the major compatible solutes in the extremely halophilic methanogen Methanohalophilus strain Z7302.

    abstract::Methanohalophilus strain Z7302 was previously isolated from a hypersaline environment and grows over a range of NaCl concentrations from 1.7 to 4.4 M. We examined the relationships between cell growth rate, cell volume, and intracellular solute concentrations with increasing salinity. This extremely halophilic methano...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.22.7474-7477.1992

    authors: Lai MC,Gunsalus RP

    更新日期:1992-11-01 00:00:00

  • BIOCHEMICAL STUDIES OF THE SMOOTH-ROUGH MUTATION IN SALMONELLA MINNESOTA.

    abstract::Lüderitz, O. (Max-Planck-Institut für Immunbiologie, Frieburg, Germany), H. J. Risse, H. Schulte-Holthausen, J. L. Strominger, I. W. Sutherland, and O. Westphal. Biochemical studies of the smooth-rough mutation in Salmonella minnesota. J. Bacteriol. 89:343-354. 1965.-A comparative study of the O antigen from the smoot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.2.343-354.1965

    authors: LUEDERITZ O,RISSE HJ,SCHULTE-HOLTHAUSEN H,STROMINGER JL,SUTHERLAND IW,WESTPHAL O

    更新日期:1965-02-01 00:00:00

  • Transport of vitamin B12 in Escherichia coli: common receptor system for vitamin B12 and bacteriophage BF23 on the outer membrane of the cell envelope.

    abstract::We showed previously that the outer membrane of the Escherichia coli cell envelope normally contains about 200 to 250 B12 receptors, and that these receptors function both in B12 transport and as receptors for the E colicins. This paper shows that this receptor system is also shared with bacteriophage BF23. A strong p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.3.1032-1039.1976

    authors: Bradbeer C,Woodrow ML,Khalifah LI

    更新日期:1976-03-01 00:00:00

  • VirB3 to VirB6 and VirB8 to VirB11, but not VirB7, are essential for mediating persistence of Brucella in the reticuloendothelial system.

    abstract::The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00406-08

    authors: den Hartigh AB,Rolán HG,de Jong MF,Tsolis RM

    更新日期:2008-07-01 00:00:00

  • Characteristics of penicillinase secretion by growing cells and protoplasts of Bacillus licheniformis.

    abstract::Cultures of the inducible penicillinase-producing strain 749 of Bacillus licheniformis, induced with small amounts of benzylpenicillin, synthesized penicillinase at a high rate for a short period, after which the rate of synthesis slowly declined. During the period of active synthesis, the rate of secretion, as a frac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.820-826.1969

    authors: Sargent MG,Ghosh BK,Lampen JO

    更新日期:1969-02-01 00:00:00

  • Effect of bacteriophage lambda infection on synthesis of groE protein and other Escherichia coli proteins.

    abstract::We used two-dimensional gel electrophoresis to quantitate the changes in rates of synthesis that follow phage lambda infection for 21 Escherichia coli proteins, including groE and dnaK proteins. Although total protein synthesis and the rates of synthesis of most individual E. coli proteins decreased after infection, s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.1050-1063.1982

    authors: Drahos DJ,Hendrix RW

    更新日期:1982-03-01 00:00:00

  • Temperature-dependent hypermutational phenotype in recA mutants of Thermus thermophilus HB27.

    abstract::The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (deltarecA) derivatives. Transcription of recA in this extreme thermophile was induced by mitomycin C, leading to the synthesis of a monocistronic mRNA. This DNA damage-mediated induction was dependent o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.16.4901-4907.2003

    authors: Castán P,Casares L,Barbé J,Berenguer J

    更新日期:2003-08-01 00:00:00

  • The plant pathogen Pantoea ananatis produces N-acylhomoserine lactone and causes center rot disease of onion by quorum sensing.

    abstract::A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. anana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01054-07

    authors: Morohoshi T,Nakamura Y,Yamazaki G,Ishida A,Kato N,Ikeda T

    更新日期:2007-11-01 00:00:00

  • Two extracytoplasmic function sigma subunits, sigma(E) and sigma(FecI), of Escherichia coli: promoter selectivity and intracellular levels.

    abstract::The promoter selectivity of two extracytoplasmic function (ECF) subfamily sigma subunits, sigma(E) (sigma(24)) and sigma(FecI) (sigma(18)), of Escherichia coli RNA polymerase was analyzed by using an in vitro transcription system and various promoters. The Esigma(E) holoenzyme recognized only the known cognate promote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.4.1181-1184.2000

    authors: Maeda H,Jishage M,Nomura T,Fujita N,Ishihama A

    更新日期:2000-02-01 00:00:00

  • Characterization of the Pseudomonas sp. strain P51 gene tcbR, a LysR-type transcriptional activator of the tcbCDEF chlorocatechol oxidative operon, and analysis of the regulatory region.

    abstract::Plasmid pP51 of Pseudomonas sp. strain P51 contains two gene clusters encoding the degradation of chlorinated benzenes, tcbAB and tcbCDEF. A regulatory gene, tcbR, was located upstream and divergently transcribed from the chlorocatechol oxidative gene cluster tcbCDEF. The tcbR gene was characterized by DNA sequencing ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3700-3708.1991

    authors: van der Meer JR,Frijters AC,Leveau JH,Eggen RI,Zehnder AJ,de Vos WM

    更新日期:1991-06-01 00:00:00

  • An ATP-dependent L-carnitine transporter in Listeria monocytogenes Scott A is involved in osmoprotection.

    abstract::Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can contribute significantly to growth of L. monocytogenes at high osmolarity (R. R. Beumer, M. C. te Giffel, L. J. Cox, F....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.11.3205-3212.1995

    authors: Verheul A,Rombouts FM,Beumer RR,Abee T

    更新日期:1995-06-01 00:00:00

  • Two Escherichia coli chromosomal cistrons, sfrA and sfrB, which are needed for expression of F factor tra functions.

    abstract::Twelve mutants of Escherichia coli K-12 have been isolated which carry chromosomal mutations that exhibit pleiotropic effects on the expression of F factor tra cistrons. F pilus synthesis, deoxyribonucleic acid transfer, and surface exclusion are all inhibited. Six of the mutants carry sfrA mutations, and six carry sf...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.3.730-737.1979

    authors: Beutin L,Achtman M

    更新日期:1979-09-01 00:00:00

  • The Conserved DNA Binding Protein WhiA Influences Chromosome Segregation in Bacillus subtilis.

    abstract::The DNA binding protein WhiA is conserved in Gram-positive bacteria and is present in the genetically simple cell wall-lacking mycoplasmas. The protein shows homology to eukaryotic homing endonucleases but lacks nuclease activity. WhiA was first characterized in streptomycetes, where it regulates the expression of key...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00633-17

    authors: Bohorquez LC,Surdova K,Jonker MJ,Hamoen LW

    更新日期:2018-03-26 00:00:00

  • Biochemical and phylogenetic characterization of a novel diaminopimelate biosynthesis pathway in prokaryotes identifies a diverged form of LL-diaminopimelate aminotransferase.

    abstract::A variant of the diaminopimelate (DAP)-lysine biosynthesis pathway uses an LL-DAP aminotransferase (DapL, EC 2.6.1.83) to catalyze the direct conversion of L-2,3,4,5-tetrahydrodipicolinate to LL-DAP. Comparative genomic analysis and experimental verification of DapL candidates revealed the existence of two diverged fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01381-07

    authors: Hudson AO,Gilvarg C,Leustek T

    更新日期:2008-05-01 00:00:00

  • A type II protein secretory pathway required for levansucrase secretion by Gluconacetobacter diazotrophicus.

    abstract::The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10) to utilize plant sucrose. LsdA, unlike other extracellular levansucrases from gram-negative bacteria, is transported to the periplasm by a signal-peptide-dependent pathway. We identified an u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.15.5031-5039.2004

    authors: Arrieta JG,Sotolongo M,Menéndez C,Alfonso D,Trujillo LE,Soto M,Ramírez R,Hernández L

    更新日期:2004-08-01 00:00:00

  • HmuP is a coactivator of Irr-dependent expression of heme utilization genes in Bradyrhizobium japonicum.

    abstract::Utilization of heme as an iron source by Bradyrhizobium japonicum involves induction of the outer membrane heme receptor gene hmuR and other genes within the heme utilization locus. Here, we discovered the hmuP gene located upstream of hmuR and transcribed divergently from it along with hmuTUV. hmuP encodes a small pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00071-12

    authors: Escamilla-Hernandez R,O'Brian MR

    更新日期:2012-06-01 00:00:00

  • Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2).

    abstract::Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.2.416-421.1975

    authors: Schrempf H,Bujard H,Hopwood DA,Goebel W

    更新日期:1975-02-01 00:00:00

  • The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite.

    abstract::The Bacillus subtilis skin element confers resistance to arsenate and arsenite. The ars operon in the skin element contains four genes in the order arsR, ORF2, arsB, and arsC. Three of these genes are homologous to the arsR, arsB, and arsC genes from the staphylococcal plasmid pI258, while no homologs of ORF2 have bee...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1655-1661.1998

    authors: Sato T,Kobayashi Y

    更新日期:1998-04-01 00:00:00

  • Genetic and biochemical characterization of a high-affinity betaine uptake system (BusA) in Lactococcus lactis reveals a new functional organization within bacterial ABC transporters.

    abstract::The cytoplasmic accumulation of exogenous betaine stimulates the growth of Lactococcus lactis cultivated under hyperosmotic conditions. We report that L. lactis possesses a single betaine transport system that belongs to the ATP-binding cassette (ABC) superfamily of transporters. Through transposon mutagenesis, a muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.20.6238-6246.1999

    authors: Obis D,Guillot A,Gripon JC,Renault P,Bolotin A,Mistou MY

    更新日期:1999-10-01 00:00:00

  • The Saccharomyces cerevisiae MEC1 gene, which encodes a homolog of the human ATM gene product, is required for G1 arrest following radiation treatment.

    abstract::The Saccharomyces cerevisiae gene MEC1 represents a structural homolog of the human gene ATM mutated in ataxia telangiectasia patients. Like human ataxia telangiectasia cell lines, mec1 mutants are defective in G2 and S-phase cell cycle checkpoints in response to radiation treatment. Here we show an additional defect ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5841-5843.1996

    authors: Siede W,Allen JB,Elledge SJ,Friedberg EC

    更新日期:1996-10-01 00:00:00

  • Genetics and regulation of peptidase N in Escherichia coli K-12.

    abstract::Escherichia coli K-12 strains contain a cytoplasmic activity, peptidase N, capable of hydrolyzing alanine-p-nitroanilide. Mutations in the structural gene for the enzyme, pepN, were mapped, and the properties of mutant strains were examined. The pepN locus lay between ompF and asnS at approximately 20.8 min on the E. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: McCaman MT,McPartland A,Villarejo MR

    更新日期:1982-11-01 00:00:00

  • Effect of ribonuclease on the association of deoxyribonucleic acid with the membrane in Escherichia coli.

    abstract::The Mg-2+-Sarkosyl crystals (M band) procedure was used to study the effect of ribonuclease (RNase) A on the association of Escherichia coli deoxyribonucleic acid (DNA) with membrane. Incubation of gently prepared cell extracts with RNase results in the release of DNA from membrane. This effect appears to result from ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.2.592-598.1975

    authors: McIntosh MA,Earhart CF

    更新日期:1975-05-01 00:00:00

  • Aerobactin utilization by Neisseria gonorrhoeae and cloning of a genomic DNA fragment that complements Escherichia coli fhuB mutations.

    abstract::Aerobactin, a dihydroxamate siderophore produced by many strains of enteric bacteria, stimulated the growth of Neisseria gonorrhoeae FA19 and F62 in iron-limiting medium. However, gonococci did not produce detectable amounts of aerobactin in the Escherichia coli LG1522 aerobactin bioassay. We probed gonococcal genomic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.8.3414-3421.1987

    authors: West SE,Sparling PF

    更新日期:1987-08-01 00:00:00

  • Tn5401, a new class II transposable element from Bacillus thuringiensis.

    abstract::A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2835-2845.1994

    authors: Baum JA

    更新日期:1994-05-01 00:00:00

  • The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.

    abstract::Two temperature-sensitive mutations, ams-1 and rne-3071, in the ams (altered mRNA stability) gene have been used extensively to investigate the processing and decay of RNA in Escherichia coli. We have sequenced these temperature-sensitive alleles and found that the mutations are separated by only 6 nucleotides and cau...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.13.4245-4249.1993

    authors: McDowall KJ,Hernandez RG,Lin-Chao S,Cohen SN

    更新日期:1993-07-01 00:00:00

  • Promoter of the pertussis toxin operon and production of pertussis toxin.

    abstract::Pertussis toxin (PT), the major virulence factor of Bordetella pertussis, is composed of five different subunits whose genes are organized as an operon. We report the mapping of the promoter region of the PT operon and show that this promoter is only weakly active in Escherichia coli. Bordetella parapertussis and Bord...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2843-2846.1987

    authors: Nicosia A,Rappuoli R

    更新日期:1987-06-01 00:00:00

  • Transformation of Kluyveromyces fragilis.

    abstract::For the transformation of the yeast species Kluyveromyces fragilis, we have constructed a vector containing a bacterial kanamycin resistance (Kmr) gene, the TRP1 gene of Saccharomyces cerevisiae, and an autonomously replicating sequence of Kluyveromyces lactis called KARS2 . By utilizing the method based on treatment ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.1165-1167.1984

    authors: Das S,Kellermann E,Hollenberg CP

    更新日期:1984-06-01 00:00:00