Two extracytoplasmic function sigma subunits, sigma(E) and sigma(FecI), of Escherichia coli: promoter selectivity and intracellular levels.

abstract：:An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruptio...

journal_title：Journal of bacteriology

authors： Earl AM,Rankin SK,Kim KP,Lamendola ON,Battista JR

更新日期：2002-02-01 00:00:00

abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...

journal_title：Journal of bacteriology

authors： Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

更新日期：2010-04-01 00:00:00

abstract：:In vitro complementation assays have been used to demonstrate the induction of alkane hydroxylase proteins in mutants lacking the ability to convert n-alkanes to their primary alcohols. Purified heptane is an effective inducer in a mutant lacking detectable hydroxylase activity. ...

journal_title：Journal of bacteriology

authors： Benson S,Shapiro J

更新日期：1975-08-01 00:00:00

abstract：:A colorimetric assay was developed for studying the kinetics of iron oxidation with whole cells of the chemoautotroph, Ferrobacillus ferrooxidans. The assay was more advantageous than the conventional method of Warburg manometry because of its simplicity, rapidity, and the small amount of cells required. The assay mea...

journal_title：Journal of bacteriology

authors： Schnaitman CA,Korczynski MS,Lundgren DG

更新日期：1969-08-01 00:00:00

abstract：:Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most gram-negative bacteria, and its structure and biosynthetic pathway are well known. Nevertheless, the mechanisms of transport and assembly of this molecule at the cell surface are poorly understood. The inner membrane (IM) transport p...

journal_title：Journal of bacteriology

authors： Sperandeo P,Lau FK,Carpentieri A,De Castro C,Molinaro A,Dehò G,Silhavy TJ,Polissi A

更新日期：2008-07-01 00:00:00

abstract：:Izaki, Kazuo (University of Tokyo, Tokyo, Japan), Kan Kiuchi, and Kei Arima. Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bacteriol. 91:628-633. 1966.-A decrease in the uptake of tetracycline occurred concurrently with a rise in the level of resistanc...

journal_title：Journal of bacteriology

authors： Izaki K,Kiuchi K,Arima K

更新日期：1966-02-01 00:00:00

abstract：:Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modu...

journal_title：Journal of bacteriology

authors： Basu J,Mahapatra S,Kundu M,Mukhopadhyay S,Nguyen-Distèche M,Dubois P,Joris B,Van Beeumen J,Cole ST,Chakrabarti P,Ghuysen JM

更新日期：1996-03-01 00:00:00

abstract：:Integrons are genetic elements that contribute to lateral gene transfer in bacteria as a consequence of possessing a site-specific recombination system. This system facilitates the spread of genes when they are part of mobile cassettes. Most integrons are contained within chromosomes and are confined to specific bacte...

journal_title：Journal of bacteriology

authors： Stokes HW,Nesbø CL,Holley M,Bahl MI,Gillings MR,Boucher Y

更新日期：2006-08-01 00:00:00

abstract：:A naturally occurring constitutive macrolide-lincosamide-streptogramin B (MLS) resistance plasmid, pNE131, from Staphylococcus epidermidis was chosen to study the molecular basis of constitutive expression. Restriction and functional maps of pNE131 are presented along with the nucleotide sequence of ermM, the gene whi...

journal_title：Journal of bacteriology

authors： Lampson BC,Parisi JT

更新日期：1986-05-01 00:00:00

abstract：:Rickettsia typhi, the causative agent of murine typhus, is an obligate intracellular bacterium with a life cycle involving both vertebrate and invertebrate hosts. Here we present the complete genome sequence of R. typhi (1,111,496 bp) and compare it to the two published rickettsial genome sequences: R. prowazekii and ...

journal_title：Journal of bacteriology

authors： McLeod MP,Qin X,Karpathy SE,Gioia J,Highlander SK,Fox GE,McNeill TZ,Jiang H,Muzny D,Jacob LS,Hawes AC,Sodergren E,Gill R,Hume J,Morgan M,Fan G,Amin AG,Gibbs RA,Hong C,Yu XJ,Walker DH,Weinstock GM

更新日期：2004-09-01 00:00:00

abstract：:In Caulobacter crescentus biogenesis of the flagellar organelle occurs during one stage of its complex life cycle. Thus in synchronous cultures it is possible to assay the sequential synthesis and assembly of the flagellum and hook in vivo with a combination of biochemical and radioimmunological techniques. The period...

journal_title：Journal of bacteriology

authors： Lagenaur C,Agabian N

更新日期：1978-09-01 00:00:00

abstract：:The characteristics of a system for the in vitro synthesis of mitochondrial deoxyribonucleic acid (mtDNA) in mitochondria isolated from Saccharomyces cerevisiae are described. In this system the exclusive product of the reaction is mtDNA. Under optimal conditions the initial rate of synthesis is close to the calculate...

journal_title：Journal of bacteriology

authors： Mattick JS,Hall RM

更新日期：1977-06-01 00:00:00

abstract：:Normal animal sera inhibit at least one Clostridium histolyticum proteinase. An assay procedure based on immune hemolysis was developed for the estimation of this inhibition. This inhibitory activity occurs in various levels in the sera of different animal species. The highest titers have been obtained with rat sera. ...

journal_title：Journal of bacteriology

authors： Luzzati A,Goldlust MB,Levine L

更新日期：1968-12-01 00:00:00

abstract：:The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibo...

journal_title：Journal of bacteriology

authors： Zhang YX,Shi Y,Zhou M,Petsko GA

更新日期：1994-02-01 00:00:00

abstract：:Using nitrogen-fixing Sinorhizobium species that interact with Medicago plants as a model system, we aimed at clarifying how sex has shaped the diversity of bacteria associated with the genus Medicago on the interspecific and intraspecific scales. To gain insights into the diversification of these symbionts, we inferr...

journal_title：Journal of bacteriology

authors： Bailly X,Olivieri I,Brunel B,Cleyet-Marel JC,Béna G

更新日期：2007-07-01 00:00:00

abstract：:Vibrio cholerae, the causative agent of the human diarrheal disease cholera, is a motile bacterium with a single polar flagellum. Motility has been implicated as a virulence determinant in some animal models of cholera, but the relationship between motility and virulence has not yet been clearly defined. We have begun...

journal_title：Journal of bacteriology

authors： Klose KE,Mekalanos JJ

更新日期：1998-01-01 00:00:00

abstract：:A previous study of the aerotolerant bacterium Lactobacillus plantarum, which lacks superoxide dismutase (SOD), demonstrated that it possesses a novel substitute for this defensive enzyme. Thus, L. plantarum contains 20 to 25 mM Mn(II),m in a dialyzable form, which is able to scavenge O2- apparently as effectively as ...

journal_title：Journal of bacteriology

authors： Archibald FS,Fridovich I

更新日期：1981-06-01 00:00:00

abstract：:Type 1 secretion systems (T1SS) are present in a wide range of Gram-negative bacteria and are involved in the secretion of diverse substrates such as proteases, lipases, and hemophores. T1SS consist of three proteins: an inner membrane ABC (ATP binding cassette) protein, a periplasmic adaptor, and an outer membrane ch...

journal_title：Journal of bacteriology

authors： Masi M,Wandersman C

更新日期：2010-08-01 00:00:00

abstract：:Salmonella typhimurium contains a transport system for sn-glycerol-3-phosphate that is inducible by growth on glycerol and sn-glycerol-3-phosphate. In fully induced cells, the system exhibited an apparent Km of 50 microM and a Vmax of 2.2 nmol/min . 10(8) cells. The corresponding system in Escherichia coli exhibits, u...

journal_title：Journal of bacteriology

authors： Hengge R,Larson TJ,Boos W

更新日期：1983-07-01 00:00:00

abstract：:The complete nucleotide sequence of the hutH gene, encoding histidine ammonia-lyase (histidase), in Pseudomonas putida ATCC 12633 has been determined from the appropriate portions of the hut region that had been cloned into Escherichia coli. The resulting DNA sequence revealed an open reading frame of 1,530 base pairs...

journal_title：Journal of bacteriology

authors： Consevage MW,Phillips AT

更新日期：1990-05-01 00:00:00

abstract：:Nickel-deficient (Nic-) mutants of Alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. The Nic- mutants carried defined deletions in the hydrogenase gene cluster of the indigenous pHG megaplasmid. Nickel deficiency correlated with a low level of the nicke...

journal_title：Journal of bacteriology

authors： Eberz G,Eitinger T,Friedrich B

更新日期：1989-03-01 00:00:00

abstract：:Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 ...

journal_title：Journal of bacteriology

authors： Kloos DU,DiMarco AA,Elsemore DA,Timmis KN,Ornston LN

更新日期：1995-10-01 00:00:00

abstract：:High-pressure liquid chromatography of a muramidase digest of murein sacculi from Caulobacter crescentus showed that the absence of D-alanine carboxypeptidase activity in the cells was reflected by a very high content of pentapeptide in the murein. Approximately half of the pentapeptide side chains were shown to conta...

journal_title：Journal of bacteriology

authors： Markiewicz Z,Glauner B,Schwarz U

更新日期：1983-11-01 00:00:00

abstract：:The ultrastructure of seven Thiobacillus species was studied. The structure of their cell envelopes is similar, if not identical, to that found in other gram-negative bacteria. Obvious differences were noted in the middle layer of the cell envelope of the seven cultures. Polyhedral inclusion bodies were apparent in fo...

journal_title：Journal of bacteriology

authors： Shively JM,Decker GL,Greenawalt JW

更新日期：1970-02-01 00:00:00

abstract：:Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...

journal_title：Journal of bacteriology

authors： Friedberg D,Platko JV,Tyler B,Calvo JM

更新日期：1995-03-01 00:00:00

abstract：:The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...

journal_title：Journal of bacteriology

authors： Stitt BL,Mosig G

更新日期：1989-07-01 00:00:00

abstract：:In Bacillus subtilis, extracellular peptide signaling regulates several biological processes. Secreted Phr signaling peptides are imported into the cell and act intracellularly to antagonize the activity of regulators known as Rap proteins. B. subtilis encodes several Rap proteins and Phr peptides, and the processes r...

journal_title：Journal of bacteriology

authors： Auchtung JM,Lee CA,Grossman AD

更新日期：2006-07-01 00:00:00

abstract：:Streptococcus suis serotype 2 (S. suis 2) is an important swine and human pathogen responsible for septicemia and meningitis. A novel gene, designated atl and encoding a major autolysin of S. suis 2 virulent strain HA9801, was identified and characterized in this study. The Atl protein contains 1,025 amino acids with ...

journal_title：Journal of bacteriology

authors： Ju CX,Gu HW,Lu CP

更新日期：2012-03-01 00:00:00

abstract：:A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during growth affected the eff...

journal_title：Journal of bacteriology

authors： Katsumata R,Ozaki A,Oka T,Furuya A

更新日期：1984-07-01 00:00:00

abstract：:Periplasmic membrane fusion proteins (MFPs) are essential components of multidrug efflux pumps and type I protein secretion systems of gram-negative bacteria. Located in the periplasm, MFPs function by creating a physical link between inner membrane transporters and outer membrane channels. The most conserved sequence...

journal_title：Journal of bacteriology

authors： Ge Q,Yamada Y,Zgurskaya H

更新日期：2009-07-01 00:00:00