Distance between alleles as a determinant of linkage in natural transformation of Acinetobacter calcoaceticus.

Abstract:

:Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 kb. Ligation of unlinked chromosomal DNA fragments allowed them to be linked genetically through natural transformation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kloos DU,DiMarco AA,Elsemore DA,Timmis KN,Ornston LN

doi

10.1128/jb.177.20.6015-6017.1995

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

6015-7

issue

20

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

杂志文章
  • Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H.

    abstract::The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced. The MRI-encoding mcr operon (mcrBDCGA) has been located immediately upstream from the mtr operon (mtrEDCBA) that encodes N5-methyltetrahydromethanopterin:c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.20.6384-6391.1994

    authors: Pihl TD,Sharma S,Reeve JN

    更新日期:1994-10-01 00:00:00

  • Identification of Campylobacter jejuni ATCC 43431-specific genes by whole microbial genome comparisons.

    abstract::This study describes a novel approach to identify unique genomic DNA sequences from the unsequenced strain C. jejuni ATCC 43431 by comparison with the sequenced strain C. jejuni NCTC 11168. A shotgun DNA microarray was constructed by arraying 9,600 individual DNA fragments from a C. jejuni ATCC 43431 genomic library o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.14.4781-4795.2004

    authors: Poly F,Threadgill D,Stintzi A

    更新日期:2004-07-01 00:00:00

  • Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici.

    abstract::Lyons, A. J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and T. G. Pridham. Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici. J. Bacteriol. 83:370-380. 1962.-It is proposed that strain ATCC 3004 (IMRU 3004) be designated hen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.370-380.1962

    authors: LYONS AJ Jr,PRIDHAM TG

    更新日期:1962-02-01 00:00:00

  • Identification of an Escherichia coli operon required for formation of the O-antigen capsule.

    abstract::Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5259-5266.2005

    authors: Peleg A,Shifrin Y,Ilan O,Nadler-Yona C,Nov S,Koby S,Baruch K,Altuvia S,Elgrably-Weiss M,Abe CM,Knutton S,Saper MA,Rosenshine I

    更新日期:2005-08-01 00:00:00

  • Role of the Nfo (YqfS) and ExoA apurinic/apyrimidinic endonucleases in protecting Bacillus subtilis spores from DNA damage.

    abstract::The Bacillus subtilis enzymes ExoA and Nfo (originally termed YqfS) are endonucleases that can repair apurinic/apyrimidinic (AP) sites and strand breaks in DNA. We have analyzed how the lack of ExoA and Nfo affects the resistance of growing cells and dormant spores of B. subtilis to a variety of treatments, some of wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.21.7374-7381.2005

    authors: Salas-Pacheco JM,Setlow B,Setlow P,Pedraza-Reyes M

    更新日期:2005-11-01 00:00:00

  • Occurrence of a class II restriction endonuclease in Staphylococcus aureus.

    abstract::The occurrence of class II restriction endonucleases (enzymes that both recognize and cleave a specific nucleotide sequence in deoxyribonucleic acid [DNA] in Staphylococcus aureus has been investigated by analysis of crude extracts obtained from different propagating strains of the International Phage Typing System. O...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.2.645-649.1977

    authors: Stobberingh EE,Schiphof R,Sussenbach JS

    更新日期:1977-08-01 00:00:00

  • Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein.

    abstract::The Vibrio fischeri luminescence (lux) genes are regulated by the 250-amino-acid-residue LuxR protein and a V. fischeri metabolite termed autoinducer. The V. fischeri lux regulon consists of two divergently transcribed units. Autoinducer and LuxR activate transcription of the luxICDABE operon and autoregulate the luxR...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.12.4064-4069.1992

    authors: Choi SH,Greenberg EP

    更新日期:1992-06-01 00:00:00

  • Induction and control of the autolytic system of Escherichia coli.

    abstract::Various methods of inducing autolysis of Escherichia coli cells were investigated, some being described here for the first time. For the autolysis of growing cells only induction methods interfering with the biosynthesis of peptidoglycan were taken into consideration, whereas with harvested cells autolysis was induced...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Leduc M,Kasra R,van Heijenoort J

    更新日期:1982-10-01 00:00:00

  • Return of Streptococcus faecalis DNA cloned in Escherichia coli to its original host via transformation of Streptococcus sanguis followed by conjugative mobilization.

    abstract::Cloning vectors were introduced into Streptococcus faecalis by conjugation. A conjugative plasmid (pVA797) and cloning vector pVA838 recombined in Streptococcus sanguis at homologous sequences, forming a cointegrate. The pVA797::pVA838 cointegrate transferred to S. faecalis by conjugation. Recombination between homolo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.3.1109-1114.1984

    authors: Smith MD,Clewell DB

    更新日期:1984-12-01 00:00:00

  • Toxicity of Pasteurella tularensis killed by ionizing radiation.

    abstract::Approximately 2 x 10(11) viable Pasteurella tularensis cells per ml, contained in suspensions, were killed by exposure to 10(6) r of gamma-radiation. When injected intraperitoneally into mice, the irradiated suspensions initially contained about 10 ld(50) per ml, and immunized mice against challenge with fully virulen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Landay ME,Wright GG,Pulliam JD,Finegold MJ

    更新日期:1968-09-01 00:00:00

  • The caulobacter Tol-Pal complex is essential for outer membrane integrity and the positioning of a polar localization factor.

    abstract::Cell division in Caulobacter crescentus involves constriction and fission of the inner membrane (IM) followed about 20 min later by fission of the outer membrane (OM) and daughter cell separation. In contrast to Escherichia coli, the Caulobacter Tol-Pal complex is essential. Cryo-electron microscopy images of the Caul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00607-10

    authors: Yeh YC,Comolli LR,Downing KH,Shapiro L,McAdams HH

    更新日期:2010-10-01 00:00:00

  • Pyrrolidone carboxylyl peptidase in Streptococcus cremoris: dependence on an interaction with membrane components.

    abstract::A study of the distribution of pyrrolidone carboxylyl peptidase (PCP) activity among cell fractions of Streptococcus cremoris HP revealed that this enzyme is associated with a particulate fraction, which mainly consists of membrane material. This location could only be established using a gentle nonmechanical method f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1281-1288.1977

    authors: Exterkate FA

    更新日期:1977-03-01 00:00:00

  • Meeting report on the ASM Conference on Mechanisms of Interbacterial Cooperation and Competition.

    abstract::The ASM Conference on Mechanisms of Interbacterial Cooperation and Competition was held in Washington DC, from 1 to 4 March 2017. The conference provided an international forum for sociomicrobiologists from different disciplines to present and discuss new findings. The meeting covered a wide range of topics, spanning ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00403-17

    authors: Lories B,Parijs I,Foster KR,Steenackers HP

    更新日期:2017-08-14 00:00:00

  • Isolation of Neurospora crassa bradytrophs.

    abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1133-1136.1979

    authors: Kinsey JA

    更新日期:1979-12-01 00:00:00

  • Electron microscopy of Bacillus megaterium undergoing isolation of its nuclear bodies.

    abstract::Fitz-James, Philip (University of Western Ontario, London, Canada). Electron microscopy of Bacillus megaterium undergoing isolation of its nucelar bodies. J. Bacteriol. 87:1202-1210. 1964.-The various steps of treatment leading to the isolation of nuclear bodies were followed by thin-section electron microscopy. Nucle...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.5.1202-1210.1964

    authors: Fitz-James P

    更新日期:1964-05-01 00:00:00

  • Regulation and function of cytochrome c' in Rhodobacter sphaeroides 2.4.3.

    abstract::Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.12.4077-4085.2005

    authors: Choi PS,Grigoryants VM,Abruña HD,Scholes CP,Shapleigh JP

    更新日期:2005-06-01 00:00:00

  • Evidence for adenylate nucleotide transport (ATP-ADP translocation) in vesicles of Frankia sp. strain EAN1pec.

    abstract::Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3053-3057.1988

    authors: Tisa LS,Ensign JC

    更新日期:1988-07-01 00:00:00

  • Clostridium perfringens spore germination: characterization of germinants and their receptors.

    abstract::Clostridium perfringens food poisoning is caused by type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe), while C. perfringens-associated non-food-borne gastrointestinal (GI) diseases are caused by isolates carrying a plasmid-borne cpe gene (P-cpe). C. perfringens spores are thought to be the importan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01748-07

    authors: Paredes-Sabja D,Torres JA,Setlow P,Sarker MR

    更新日期:2008-02-01 00:00:00

  • Identification of an operon involved in sulfolipid biosynthesis in Rhodobacter sphaeroides.

    abstract::Two new mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening mutagenized cell lines for polar lipid composition by thin-layer chromatography of lipid extracts. A genomic clone which complemented the mutations in these two lines, but not the previously described su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.20.6479-6487.1992

    authors: Benning C,Somerville CR

    更新日期:1992-10-01 00:00:00

  • Denitrification by Alcaligenes eutrophus is plasmid dependent.

    abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.852-854.1985

    authors: Römermann D,Friedrich B

    更新日期:1985-05-01 00:00:00

  • Bacteriophage infection in rod-shaped gram-positive bacteria: evidence for a preferential polar route for phage SPP1 entry in Bacillus subtilis.

    abstract::Entry into the host bacterial cell is one of the least understood steps in the life cycle of bacteriophages. The different envelopes of Gram-negative and Gram-positive bacteria, with a fluid outer membrane and exposing a thick peptidoglycan wall to the environment respectively, impose distinct challenges for bacteriop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05104-11

    authors: Jakutytė L,Baptista C,São-José C,Daugelavičius R,Carballido-López R,Tavares P

    更新日期:2011-09-01 00:00:00

  • Tripartite Regulation of the glpFKD Operon Involved in Glycerol Catabolism by GylR, Crp, and SigF in Mycobacterium smegmatis.

    abstract::The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00511-19

    authors: Bong HJ,Ko EM,Song SY,Ko IJ,Oh JI

    更新日期:2019-11-20 00:00:00

  • Extracellular hemolysins of aerobic sporogenic bacilli.

    abstract::Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.5.1541-1543.1967

    authors: Bernheimer AW,Grushoff P

    更新日期:1967-05-01 00:00:00

  • Hypersensitivity to Hg2+ and hyperbinding activity associated with cloned fragments of the mercurial resistance operon of plasmid NR1.

    abstract::The region of plasmid NR1 concerned with resistance to Hg2+ and organomercurials consists of sequences found on restriction endonuclease fragments EcoRI-H and EcoRI-I. When both fragments were cloned together into a derivative of plasmid ColE1, the hybrid plasmid conferred properties indistinguishable from those of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.1.161-166.1979

    authors: Nakahara H,Silver S,Miki T,Rownd RH

    更新日期:1979-10-01 00:00:00

  • Chromosome rearrangement and diversification of Francisella tularensis revealed by the type B (OSU18) genome sequence.

    abstract::The gamma-proteobacterium Francisella tularensis is one of the most infectious human pathogens, and the highly virulent organism F. tularensis subsp. tularensis (type A) and less virulent organism F. tularensis subsp. holarctica (type B) are most commonly associated with significant disease in humans and animals. Here...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00506-06

    authors: Petrosino JF,Xiang Q,Karpathy SE,Jiang H,Yerrapragada S,Liu Y,Gioia J,Hemphill L,Gonzalez A,Raghavan TM,Uzman A,Fox GE,Highlander S,Reichard M,Morton RJ,Clinkenbeard KD,Weinstock GM

    更新日期:2006-10-01 00:00:00

  • Stringent control of peptidoglycan biosynthesis in Escherichia coli K-12.

    abstract::[3H]Diaminopimelic acid (Dap) was incorporated exclusively into peptidoglycan by Escherichia coli strains auxotrophic for both lysine and Dap. The rate of [3H]Dap incorporation by stringent (rel+) strains was significantly decreased when cells were deprived of required amino acids. The addition of chloramphenicol to a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1119-1126.1976

    authors: Ishiguro EE,Ramey WD

    更新日期:1976-09-01 00:00:00

  • Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.

    abstract::Microarray-based analysis of the transcriptional profiles of the genetically distinct Staphylococcus aureus strains COL, GP268, and Newman indicate that a total of 251 open reading frames (ORFs) are influenced by sigmaB activity. While sigmaB was found to positively control 198 genes by a factor of > or =2 in at least...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.13.4085-4099.2004

    authors: Bischoff M,Dunman P,Kormanec J,Macapagal D,Murphy E,Mounts W,Berger-Bächi B,Projan S

    更新日期:2004-07-01 00:00:00

  • Highly conjugative pMG1-like plasmids carrying Tn1546-like transposons that encode vancomycin resistance in Enterococcus faecium.

    abstract::A total of 12 VanA-type vancomycin-resistant enterococci, consisting of 10 Enterococcus faecium isolates and two Enterococcus avium isolates, were examined in detail. The vancomycin resistance conjugative plasmids pHTalpha (65.9 kbp), pHTbeta (63.7 kbp), and pHTgamma (66.5 kbp) were isolated from each of three differe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.23.7024-7028.2003

    authors: Tomita H,Tanimoto K,Hayakawa S,Morinaga K,Ezaki K,Oshima H,Ike Y

    更新日期:2003-12-01 00:00:00

  • Permeability of Pseudomonas aeruginosa outer membrane to hydrophilic solutes.

    abstract::Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydroly...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Yoshimura F,Nikaido H

    更新日期:1982-11-01 00:00:00

  • icaR encodes a transcriptional repressor involved in environmental regulation of ica operon expression and biofilm formation in Staphylococcus epidermidis.

    abstract::Biofilm formation in Staphylococcus epidermidis is dependent upon the ica operon-encoded polysaccharide intercellular adhesin, which is subject to phase-variable and environmental regulation. The icaR gene, located adjacent to the ica operon, appears to be a member of the tetR family of transcriptional regulators. In ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.16.4400-4408.2002

    authors: Conlon KM,Humphreys H,O'Gara JP

    更新日期:2002-08-01 00:00:00