Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.

Abstract:

:Microarray-based analysis of the transcriptional profiles of the genetically distinct Staphylococcus aureus strains COL, GP268, and Newman indicate that a total of 251 open reading frames (ORFs) are influenced by sigmaB activity. While sigmaB was found to positively control 198 genes by a factor of > or =2 in at least two of the three genetic lineages analyzed, 53 ORFs were repressed in the presence of sigmaB. Gene products that were found to be influenced by sigmaB are putatively involved in all manner of cellular processes, including cell envelope biosynthesis and turnover, intermediary metabolism, and signaling pathways. Most of the genes and/or operons identified as upregulated by sigmaB were preceded by a nucleotide sequence that resembled the sigmaB consensus promoter sequence of Bacillus subtilis. A conspicuous number of virulence-associated genes were identified as regulated by sigmaB activity, with many adhesins upregulated and prominently represented in this group, while transcription of various exoproteins and toxins were repressed. The data presented here suggest that the sigmaB of S. aureus controls a large regulon and is an important modulator of virulence gene expression that is likely to act conversely to RNAIII, the effector molecule of the agr locus. We propose that this alternative transcription factor may be of importance for the invading pathogen to fine-tune its virulence factor production in response to changing host environments.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bischoff M,Dunman P,Kormanec J,Macapagal D,Murphy E,Mounts W,Berger-Bächi B,Projan S

doi

10.1128/JB.186.13.4085-4099.2004

keywords:

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

4085-99

issue

13

eissn

0021-9193

issn

1098-5530

pii

186/13/4085

journal_volume

186

pub_type

杂志文章
  • Immunocytochemical localization of a calmodulinlike protein in Bacillus subtilis cells.

    abstract::To determine possible functions of the calmodulinlike protein of Bacillus subtilis, the time course of its expression during sporulation and its cellular localization were studied. The protein was expressed in a constitutive manner from the end of logarithmic growth through 8 h of sporulation as determined by antibody...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.15.4605-4610.1999

    authors: Dominguez DC,Adams H,Hageman JH

    更新日期:1999-08-01 00:00:00

  • FepA with globular domain deletions lacks activity.

    abstract::TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.19.5508-5512.2002

    authors: Vakharia HL,Postle K

    更新日期:2002-10-01 00:00:00

  • Cells of Escherichia coli contain a protein-tyrosine kinase, Wzc, and a phosphotyrosine-protein phosphatase, Wzb.

    abstract::Two proteins of Escherichia coli, termed Wzc and Wzb, were analyzed for their capacity to participate in the reversible phosphorylation of proteins on tyrosine. First, Wzc was overproduced from its specific gene and purified to homogeneity by affinity chromatography. Upon incubation in the presence of radioactive ATP,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.11.3472-3477.1999

    authors: Vincent C,Doublet P,Grangeasse C,Vaganay E,Cozzone AJ,Duclos B

    更新日期:1999-06-01 00:00:00

  • Genetic determinants of Silicibacter sp. TM1040 motility.

    abstract::Silicibacter sp. TM1040 is a member of the marine Roseobacter clade of Alphaproteobacteria that forms symbioses with unicellular eukaryotic phytoplankton, such as dinoflagellates. The symbiosis is complex and involves a series of steps that physiologically change highly motile bacteria into cells that readily form bio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00429-09

    authors: Belas R,Horikawa E,Aizawa S,Suvanasuthi R

    更新日期:2009-07-01 00:00:00

  • Selective depletion of Sulfolobus solfataricus transcription factor E under heat shock conditions.

    abstract::Archaeal transcriptional machinery is similar to that of eukaryotes. We studied the fates of various components of the Sulfolobus solfataricus transcriptional apparatus under different stresses and found that in cells incubated at 90 degrees C for 1 h, transcription factor E (TFE) is selectively depleted, but its mRNA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01534-09

    authors: Iqbal J,Qureshi SA

    更新日期:2010-06-01 00:00:00

  • Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile.

    abstract::The isolation of a new thermophilic bacterium, Thermus aquaticus gen. n. and sp. n., is described. Successful enrichment requires incubation at 70 to 75 C, and the use of nutrient media relatively dilute with respect to the organic components. Strains of T. aquaticus have been isolated from a variety of thermal spring...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.289-297.1969

    authors: Brock TD,Freeze H

    更新日期:1969-04-01 00:00:00

  • Polar remodeling and histidine kinase activation, which is essential for Caulobacter cell cycle progression, are dependent on DNA replication initiation.

    abstract::Caulobacter crescentus initiates a single round of DNA replication during each cell cycle. Following the initiation of DNA replication, the essential CckA histidine kinase is activated by phosphorylation, which (via the ChpT phosphotransferase) enables the phosphorylation and activation of the CtrA global regulator. C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00468-10

    authors: Iniesta AA,Hillson NJ,Shapiro L

    更新日期:2010-08-01 00:00:00

  • Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci.

    abstract::Degenerate oligonucleotides based on the published Escherichia coli glutamate decarboxylase (GAD) protein sequence were used in a polymerase chain reaction to generate a DNA probe for the E. coli GAD structural gene. Southern blots showed that there were two cross-hybridizing GAD genes, and both of these were cloned a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.18.5820-5826.1992

    authors: Smith DK,Kassam T,Singh B,Elliott JF

    更新日期:1992-09-01 00:00:00

  • Evidence that the clindamycin-erythromycin resistance gene of Bacteroides plasmid pBF4 is on a transposable element.

    abstract::We constructed a shuttle vector, pE5-2, which can replicate in both Bacteroides spp. and Escherichia coli. pE5-2 contains a cryptic Bacteroides plasmid (pB8-51), a 3.8-kilobase (kb) EcoRI-D fragment from the 41-kb Bacteroides fragilis plasmid pBF4, and RSF1010, an IncQ E. coli plasmid. pE5-2 was mobilized by R751, an ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.626-632.1985

    authors: Shoemaker NB,Guthrie EP,Salyers AA,Gardner JF

    更新日期:1985-05-01 00:00:00

  • Characterization of Brucella suis clpB and clpAB mutants and participation of the genes in stress responses.

    abstract::Pathogens often encounter stressful conditions inside their hosts. In the attempt to characterize the stress response in Brucella suis, a gene highly homologous to Escherichia coli clpB was isolated from Brucella suis, and the deduced amino acid sequence showed features typical of the ClpB ATPase family of stress resp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.8.2677-2681.2001

    authors: Ekaza E,Teyssier J,Ouahrani-Bettache S,Liautard JP,Köhler S

    更新日期:2001-04-01 00:00:00

  • Two divergently transcribed genes, soxR and soxS, control a superoxide response regulon of Escherichia coli.

    abstract::soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.9.2864-2871.1991

    authors: Wu J,Weiss B

    更新日期:1991-05-01 00:00:00

  • Site-specific insertion of biologically functional adventitious genes into the Streptomyces lividans chromosome.

    abstract::We report that transformation of Streptomyces lividans with cloned DNA of the SLP1 genetic element results in integration of the element at the same chromosomal locus (attB) normally occupied by SLP1 in its original host, Streptomyces coelicolor, and in S. lividans that has received SLP1 by mating. We constructed SLP1...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.5.2174-2184.1988

    authors: Omer CA,Stein D,Cohen SN

    更新日期:1988-05-01 00:00:00

  • Neisseria gonorrhoeae Crippled Its Peptidoglycan Fragment Permease To Facilitate Toxic Peptidoglycan Monomer Release.

    abstract::Neisseria gonorrhoeae (gonococci) and Neisseria meningitidis (meningococci) are human pathogens that cause gonorrhea and meningococcal meningitis, respectively. Both N. gonorrhoeae and N. meningitidis release a number of small peptidoglycan (PG) fragments, including proinflammatory PG monomers, although N. meningitidi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00437-16

    authors: Chan JM,Dillard JP

    更新日期:2016-10-07 00:00:00

  • Genome sequence of Enterococcus sp. strain C1, an azo dye decolorizer.

    abstract::Enterococcus sp. strain C1 is a facultative anaerobe which was coisolated with Citrobacter sp. strain A1 from a sewage oxidation pond. Strain C1 could degrade azo dyes very efficiently via azo reduction and desulfonation in a microaerophilic environment. Here the draft genome sequence of Enterococcus sp. C1 is reporte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01372-12

    authors: Chan GF,Gan HM,Rashid NA

    更新日期:2012-10-01 00:00:00

  • Glutamine synthetase mutations which affect expression of nitrogen fixation genes in Klebsiella pneumoniae.

    abstract::Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in Klebsiella pneumoniae. We report here the isolation of a new class of K. pneumoniae mutants which exhibit altered pattern...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.597-606.1979

    authors: Ausubel FM,Bird SC,Durbin KJ,Janssen KA,Margolskee RF,Peskin AP

    更新日期:1979-11-01 00:00:00

  • Membrane-bound cytochrome c is an alternative electron donor for cytochrome aa3 in Nitrobacter winogradskyi.

    abstract::We purified membrane-bound cytochrome c-550 [cytochrome c-550(m)] to an electrophoretically homogeneous state from Nitrobacter winogradskyi. The cytochrome showed peaks at 409 and 525 nm in the oxidized form and peaks at 416, 521, and 550 nm in the reduced form. The molecular weight of the cytochrome was estimated to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.14.4400-4404.1993

    authors: Nomoto T,Fukumori Y,Yamanaka T

    更新日期:1993-07-01 00:00:00

  • Under Elevated c-di-GMP in Escherichia coli, YcgR Alters Flagellar Motor Bias and Speed Sequentially, with Additional Negative Control of the Flagellar Regulon via the Adaptor Protein RssB.

    abstract::In Escherichia coli and Salmonella, the c-di-GMP effector YcgR inhibits flagellar motility by interacting directly with the motor to alter both its bias and speed. Here, we demonstrate that in both of these bacteria, YcgR acts sequentially, altering motor bias first and then decreasing motor speed. We show that when c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00578-19

    authors: Nieto V,Partridge JD,Severin GB,Lai RZ,Waters CM,Parkinson JS,Harshey RM

    更新日期:2019-12-06 00:00:00

  • Proteins antigenically related to methyl-accepting chemotaxis proteins of Escherichia coli detected in a wide range of bacterial species.

    abstract::The four methyl-accepting chemotaxis proteins of Escherichia coli, often called transducers, are transmembrane receptor proteins that exhibit substantial identity among the sequences of their cytoplasmic domains. Thus, antiserum raised to one of these proteins recognizes the others and might be expected to recognize r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.1.133-140.1993

    authors: Morgan DG,Baumgartner JW,Hazelbauer GL

    更新日期:1993-01-01 00:00:00

  • Population genetic analysis of Helicobacter pylori by multilocus enzyme electrophoresis: extensive allelic diversity and recombinational population structure.

    abstract::Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes encoding metabolic housekeeping enzymes by multilocus enzyme electrophoresis. Seventy-three distinct allele profiles, r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.13.3934-3938.1996

    authors: Go MF,Kapur V,Graham DY,Musser JM

    更新日期:1996-07-01 00:00:00

  • Biosynthesis of uridine diphosphate N-acetylmannosaminuronic acid in rff mutants of Salmonella tryphimurium.

    abstract::In Salmonella typhimurium, three groups of genes located in rfb, rfe, and rff clusters are known to be involved in the biosynthesis of the enterobacterial common antigen. We found that enzymatic synthesis of uridine diphosphate N-acetylmannosaminouric acid, the activated form of a constituent sugar of the common antig...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.227-233.1978

    authors: Lew HC,Nikaido H,Mäkelä PH

    更新日期:1978-10-01 00:00:00

  • Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli.

    abstract::The fec region of the Escherichia coli chromosome determines a citrate-dependent iron(III) transport system. The nucleotide sequence of fec revealed five genes, fecABCDE, which are transcribed from fecA to fecE. The fecA gene encodes a previously described outer membrane receptor protein. The fecB gene product is form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2626-2633.1989

    authors: Staudenmaier H,Van Hove B,Yaraghi Z,Braun V

    更新日期:1989-05-01 00:00:00

  • Purification and characterization of a primary-secondary alcohol dehydrogenase from two strains of Clostridium beijerinckii.

    abstract::Two primary alcohols (1-butanol and ethanol) are major fermentation products of several clostridial species. In addition to these two alcohols, the secondary alcohol 2-propanol is produced to a concentration of about 100 mM by some strains of Clostridium beijerinckii. An alcohol dehydrogenase (ADH) has been purified t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.16.5097-5105.1993

    authors: Ismaiel AA,Zhu CX,Colby GD,Chen JS

    更新日期:1993-08-01 00:00:00

  • Germination and outgrowth of single spores of Saccharomyces cerevisiae viewed by scanning electron and phase-contrast microscopy.

    abstract::Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that develop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.3.1232-1238.1972

    authors: Rousseau P,Halvorson HO,Bulla LA Jr,St Julian G

    更新日期:1972-03-01 00:00:00

  • Lag Phase Is a Dynamic, Organized, Adaptive, and Evolvable Period That Prepares Bacteria for Cell Division.

    abstract::Lag is a temporary period of nonreplication seen in bacteria that are introduced to new media. Despite latency being described by Müller in 1895, only recently have we gained insights into the cellular processes characterizing lag phase. This review covers literature to date on the transcriptomic, proteomic, metabolom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00697-18

    authors: Bertrand RL

    更新日期:2019-03-13 00:00:00

  • Characterization of Pseudomonas aeruginosa derepressed R-plasmids.

    abstract::A genetic study of conjugal transmissibility of two R-plasmids was undertaken in Pseudomonas aeruginosa. Conjugally derepressed mutants of the R-plasmids were isolated, and examination of 11 independent mutants revealed that 10 were recessive to the wild-type transfer repressor, whereas 1 mutant was cis dominant. Cros...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.2.596-603.1977

    authors: Chandler PM,Krishnapillai V

    更新日期:1977-05-01 00:00:00

  • Functional and structural analysis of HicA3-HicB3, a novel toxin-antitoxin system of Yersinia pestis.

    abstract::The mechanisms involved in the virulence of Yersinia pestis, the plague pathogen, are not fully understood. In previous research, we found that a Y. pestis mutant lacking the HicB3 (YPO3369) putative orphan antitoxin was attenuated for virulence in a murine model of bubonic plague. Toxin-antitoxin systems (TASs) are w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01932-14

    authors: Bibi-Triki S,Li de la Sierra-Gallay I,Lazar N,Leroy A,Van Tilbeurgh H,Sebbane F,Pradel E

    更新日期:2014-11-01 00:00:00

  • Active transport of D-alanine and related amino acids by whole cells of Bacillus subtilis.

    abstract::Whole cells of Bacillus subtilis transported d-alanine and l-alanine by two different systems. The high-affinity system (K(m) of 1 muM and V(max) of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (K(m) of 10 muM for l-alanine and 20 muM for d-alanine a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.3.1085-1092.1974

    authors: Clark VL,Young FE

    更新日期:1974-12-01 00:00:00

  • Change in direction of flagellar rotation in Escherichia coli mediated by acetate kinase.

    abstract::Strains of Escherichia coli lacking all cytoplasmic chemotaxis proteins except CheY swim smoothly under most conditions. However, they tumble when exposed to acetate. Acetate coenzyme A synthetase (EC 6.2.1.1) was thought to be essential for this response. New evidence suggests that the tumbling is mediated instead by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.10.3236-3239.1993

    authors: Dailey FE,Berg HC

    更新日期:1993-05-01 00:00:00

  • Bacteriocin (hemolysin) of Streptococcus zymogenes.

    abstract::The sensitivity of Streptococcus faecalis (ATTC 8043) to S. zymogenes X-14 bacteriocin depends greatly on its physiological age. Sensitivity decreases from the mid-log phase on and is completely lost in the stationary phase. The sensitivity of erythrocytes to the hemolytic capacity of the bacteriocin showed considerab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.6.1895-1902.1968

    authors: Basinger SF,Jackson RW

    更新日期:1968-12-01 00:00:00

  • A quorum-sensing system in the free-living photosynthetic bacterium Rhodobacter sphaeroides.

    abstract::Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7530-7537.1997

    authors: Puskas A,Greenberg EP,Kaplan S,Schaefer AL

    更新日期:1997-12-01 00:00:00