Germination and outgrowth of single spores of Saccharomyces cerevisiae viewed by scanning electron and phase-contrast microscopy.

Abstract:

:Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that development of the vegetative cell from a spore follows a definite sequence of events involving dramatic physical and chemical modifications. These changes are: initial rapid loss in cellular absorbance followed later by an abrupt gain in absorbance; reduction in cell weight and a subsequent progressive increase; modification of the spore surface with concomitant diminution in refractility; elongation of the cell and augmentation of surface irregularities; rapid decline in trehalose content of the cell accompanied by extensive formation of KOH-soluble carbohydrates; and bud formation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rousseau P,Halvorson HO,Bulla LA Jr,St Julian G

doi

10.1128/JB.109.3.1232-1238.1972

subject

Has Abstract

pub_date

1972-03-01 00:00:00

pages

1232-8

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

109

pub_type

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