Pyrogenic principle of the ribonucleic acid from the yeast Candida utilis.

Abstract:

:Experiments were performed to characterize the pyrogenic principle of ribonucleic acid (RNA) from the yeast Candida utilis. It was shown that ribonuclease hydrolysis of the RNA does not lead to inactivation of the pyrogenicity. Pyrogenicity was, however, destroyed by treatment with sodium deoxycholate. On column chromatography with Biogel under sterile and pyrogen-free conditions, the pyrogenic principle of yeast RNA was eluted together with the RNA. After treatment of the RNA with ribonuclease, it was possible to separate the pyrogenic activity from the RNA (hydrolysis products) to a great extent. Column chromatography of Escherichia coli endotoxin showed that the endotoxin was eluted in the same fractions as the pyrogenic activity of yeast RNA. On the basis of the behavior of the pyrogen, it may very well be that the fever reaction is produced not by the nucleic acid but by pyrogenic contaminants of the RNA preparation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kanoh S,Kohlhage H,Siegert R

doi

10.1128/JB.96.3.738-743.1968

subject

Has Abstract

pub_date

1968-09-01 00:00:00

pages

738-43

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

96

pub_type

杂志文章
  • Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids.

    abstract::Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The op...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.153-160.1979

    authors: Emerich DW,Ruiz-Argüeso T,Ching TM,Evans HJ

    更新日期:1979-01-01 00:00:00

  • Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici.

    abstract::Lyons, A. J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and T. G. Pridham. Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici. J. Bacteriol. 83:370-380. 1962.-It is proposed that strain ATCC 3004 (IMRU 3004) be designated hen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.370-380.1962

    authors: LYONS AJ Jr,PRIDHAM TG

    更新日期:1962-02-01 00:00:00

  • Isolation of Klebsiella aerogenes mutants cis-dominant for glutamine synthetase expression.

    abstract::We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glnA region and co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.2.671-679.1980

    authors: Rothstein DM,Magasanik B

    更新日期:1980-02-01 00:00:00

  • Burkholderia spp. alter Pseudomonas aeruginosa physiology through iron sequestration.

    abstract::Pseudomonas aeruginosa and members of the Burkholderia cepacia complex often coexist in both the soil and the lungs of cystic fibrosis patients. To gain an understanding of how these different species affect each other's physiology when coexisting, we performed a screen to identify P. aeruginosa genes that are induced...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.8.2376-2384.2004

    authors: Weaver VB,Kolter R

    更新日期:2004-04-01 00:00:00

  • Gut Microbiota: the Emerging Link to Lung Homeostasis and Disease.

    abstract::The gut microbiota plays a crucial role in the development of the immune system and confers benefits or disease susceptibility to the host. Emerging studies have indicated the gut microbiota could affect pulmonary health and disease through cross talk between the gut microbiota and the lungs. Gut microbiota dysbiosis c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00454-20

    authors: Zhou A,Lei Y,Tang L,Hu S,Yang M,Wu L,Yang S,Tang B

    更新日期:2021-01-25 00:00:00

  • Use of an inducible regulatory protein to identify members of a regulon: application to the regulon controlled by the leucine-responsive regulatory protein (Lrp) in Escherichia coli.

    abstract::Procedures were developed to facilitate the identification of genes that belong to a given regulon and characterization of their responses to the regulator. The regulon controlled by the Escherichia coli leucine-responsive regulatory protein (Lrp) was studied by isolating random transcriptional fusions to lacZ, using ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.20.6254-6263.1997

    authors: Bhagwat SP,Rice MR,Matthews RG,Blumenthal RM

    更新日期:1997-10-01 00:00:00

  • A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes.

    abstract::Virulence genes in Listeria monocytogenes are coordinately expressed under the control of the transcriptional activator PrfA, encoded by prfA, a member of the cyclic AMP (cAMP) receptor protein (CRP)/FNR family of bacterial regulators. Strain P14-A is a spontaneous mutant of L. monocytogenes serovar 4b which produces ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.5.1533-1540.1997

    authors: Ripio MT,Domínguez-Bernal G,Lara M,Suárez M,Vazquez-Boland JA

    更新日期:1997-03-01 00:00:00

  • Cj1386 is an ankyrin-containing protein involved in heme trafficking to catalase in Campylobacter jejuni.

    abstract::Campylobacter jejuni, a microaerophilic bacterium, is the most frequent cause of human bacterial gastroenteritis. C. jejuni is exposed to harmful reactive oxygen species (ROS) produced during its own normal metabolic processes and during infection from the host immune system and from host intestinal microbiota. These ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05740-11

    authors: Flint A,Sun YQ,Stintzi A

    更新日期:2012-01-01 00:00:00

  • TWO LYTIC FACTORS INVOLVED IN INDUCED SYNERGISTIC HEMOLYSIS IN NORMALLY NONHEMOLYTIC STAPHYLOCOCCI.

    abstract::Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.6.1700-1704.1964

    authors: SMITH DC,FOLTZ VD,LORD TH

    更新日期:1964-12-01 00:00:00

  • Implication of the VirD4 coupling protein of the Lvh type 4 secretion system in virulence phenotypes of Legionella pneumophila.

    abstract::The genome of the Philadelphia-1 strain of Legionella pneumophila, the causative organism of Legionnaires' disease, encodes two virulence-associated type 4 secretion systems (T4SSs), the Dot/Icm type 4B (T4BSS) and the Lvh type 4A (T4ASS). Broth stationary-phase cultures of most dot/icm mutants are defective in entry ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00430-13

    authors: Bandyopadhyay P,Lang EA,Rasaputra KS,Steinman HM

    更新日期:2013-08-01 00:00:00

  • Overlapping mRNA transcripts of photosynthesis gene operons in Rhodobacter capsulatus.

    abstract::The crtEF, bchCA, and puf operons of the facultative phototrophic bacterium Rhodobacter capsulatus encode gene products that are necessary for the formation of various components of the photosynthetic apparatus. The crtEF operon encodes two enzymes involved in the biosynthesis of carotenoids, the bchCA operon codes fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.4.1432-1443.1991

    authors: Wellington CL,Beatty JT

    更新日期:1991-02-01 00:00:00

  • Arm-in-Arm Response Regulator Dimers Promote Intermolecular Signal Transduction.

    abstract:UNLABELLED:Bacteriophytochrome photoreceptors (BphPs) and their cognate response regulators make up two-component signal transduction systems which direct bacteria to mount phenotypic responses to changes in environmental light quality. Most of these systems utilize single-domain response regulators to transduce signal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00872-15

    authors: Baker AW,Satyshur KA,Moreno Morales N,Forest KT

    更新日期:2016-03-31 00:00:00

  • Constancy of growth on simple and complex media.

    abstract::An apparatus has been developed in which bacterial growth can be measured very precisely over short intervals of time. Its precision is presented and used to assess the constancy of growth in batch culture. Under certain conditions, i.e., Luria broth or 0.2% glucose-M9 medium at very low cell densities, the specific g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.3.969-975.1978

    authors: Wang CH,Koch AL

    更新日期:1978-12-01 00:00:00

  • Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number.

    abstract::The trfA gene of the broad-host-range plasmid RK2 is essential for initiation of plasmid replication. Two related TrfA proteins of 43 and 32 kilodaltons (kDa) are produced by independent translation initiation at two start codons within the trfA open reading frame. These proteins were o overproduced in Escherichia col...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.3849-3858.1990

    authors: Durland RH,Helinski DR

    更新日期:1990-07-01 00:00:00

  • Trehalose metabolism by Bacillus popilliae.

    abstract::Trehalose was found to be utilized more readily than glucose for the growth of Bacillus popilliae NRRL B-2309MC. The pathway of degradation of trehalose was elucidated and found to differ from that reported for other organisms. Trehalase and trehalose phosphorylase activities could not be detected. Rather, trehalose w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.2.484-493.1974

    authors: Bhumiratana A,Anderson RL,Costilow RN

    更新日期:1974-08-01 00:00:00

  • Heterogenetic antigens of gram-positive bacteria.

    abstract::Chorpenning, Frank W. (The Ohio State University, Columbus), and Matthew C. Dodd. Heterogenetic antigens of gram-positive bacteria. J. Bacteriol. 91:1440-1445. 1966.-Soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune ra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.4.1440-1445.1966

    authors: Chorpenning FW,Dodd MC

    更新日期:1966-04-01 00:00:00

  • Selective mRNA degradation by polynucleotide phosphorylase in cold shock adaptation in Escherichia coli.

    abstract::Upon cold shock, Escherichia coli cell growth transiently stops. During this acclimation phase, specific cold shock proteins (CSPs) are highly induced. At the end of the acclimation phase, their synthesis is reduced to new basal levels, while the non-cold shock protein synthesis is resumed, resulting in cell growth re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.9.2808-2816.2001

    authors: Yamanaka K,Inouye M

    更新日期:2001-05-01 00:00:00

  • Purification, characterization, and physiological function of Bacillus subtilis ornithine transcarbamylase.

    abstract::A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.2.512-521.1983

    authors: Neway JO,Switzer RL

    更新日期:1983-08-01 00:00:00

  • Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12.

    abstract::We cloned the cryptic phn operon of a K-12 strain, phn(EcoK), and analyzed the nucleotide sequence of the phn region (11,672 bp). An mRNA start site upstream of the phnC gene was identified by S1 nuclease mapping. The pho regulon activator PhoB protects a pho box region near the mRNA start in DNase I footprinting and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2665-2672.1991

    authors: Makino K,Kim SK,Shinagawa H,Amemura M,Nakata A

    更新日期:1991-04-01 00:00:00

  • Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes.

    abstract::The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.2178-2180.1990

    authors: Aslanidis C,Schmitt R

    更新日期:1990-04-01 00:00:00

  • A Genome-Wide Helicobacter pylori Morphology Screen Uncovers a Membrane-Spanning Helical Cell Shape Complex.

    abstract::Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering prop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00724-18

    authors: Yang DC,Blair KM,Taylor JA,Petersen TW,Sessler T,Tull CM,Leverich CK,Collar AL,Wyckoff TJ,Biboy J,Vollmer W,Salama NR

    更新日期:2019-06-21 00:00:00

  • A Xanthomonas campestris pv. campestris protein similar to catabolite activation factor is involved in regulation of phytopathogenicity.

    abstract::A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.5877-5883.1990

    authors: de Crecy-Lagard V,Glaser P,Lejeune P,Sismeiro O,Barber CE,Daniels MJ,Danchin A

    更新日期:1990-10-01 00:00:00

  • Structure and Mutational Analyses of Escherichia coli ZapD Reveal Charged Residues Involved in FtsZ Filament Bundling.

    abstract:UNLABELLED:Bacterial cell division is an essential and highly coordinated process. It requires the polymerization of the tubulin homologue FtsZ to form a dynamic ring (Z-ring) at midcell. Z-ring formation relies on a group of FtsZ-associated proteins (Zap) for stability throughout the process of division. In Escherichi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00969-15

    authors: Roach EJ,Wroblewski C,Seidel L,Berezuk AM,Brewer D,Kimber MS,Khursigara CM

    更新日期:2016-05-13 00:00:00

  • Cyclic nucleotide-independent protein kinases from ribosomes and phosphorylation of a single 40S ribosomal subunit protein in zoospores of Blastocladiella emersonii.

    abstract::Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.3.1467-1471.1983

    authors: Bonato MC,da Costa Maia JC,Juliani MH

    更新日期:1983-06-01 00:00:00

  • Effects of environmental changes on expression of the oligopeptide permease (opp) genes of Borrelia burgdorferi.

    abstract::We analyzed expression of a putative oligopeptide permease (Opp) of Borrelia burgdorferi. Unlike the opp operons of other bacteria for which there is a single substrate binding protein, B. burgdorferi codes for three substrate binding proteins (OppA-I to -III) in its opp operon and an additional two homologs on plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.22.6198-6206.2002

    authors: Wang XG,Lin B,Kidder JM,Telford S,Hu LT

    更新日期:2002-11-01 00:00:00

  • Interaction of Copper Toxicity and Oxidative Stress in Campylobacter jejuni.

    abstract::Copper is both a required micronutrient and a source of toxicity in most organisms, including Campylobacter jejuni Two proteins expressed in C. jejuni (termed CopA and CueO) have been shown to be a copper transporter and multicopper oxidase, respectively. We have isolated strains with mutations in these genes, and her...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00208-18

    authors: Gardner SP,Olson JW

    更新日期:2018-10-10 00:00:00

  • Turnover of phosphatidylglycerol in Escherichia coli.

    abstract::In growing cultures of Escherichia coli, the nonacylated glycerol of phosphatidylglycerol (PG) is labeled more rapidly than is the acylated glycerol. This is, in part, due to a rapid exchange reaction of the nonacylated glycerol. Only some of the PG molecules undergo this reaction while others are stable. Using a muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.1.210-214.1973

    authors: Ballesta JP,De Garcia CL,Schaechter M

    更新日期:1973-10-01 00:00:00

  • Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane.

    abstract::Two plasmids containing the penicillinase gene of alkalophilic Bacillus sp. strain 170, pEAP1 and pEAP2, were constructed. Most of the penicillinase produced by Escherichia coli, which carried these plasmids, was found in the culture medium. This excretion is caused by the cloned DNA fragment which contains some compo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.2.949-951.1983

    authors: Kudo T,Kato C,Horikoshi K

    更新日期:1983-11-01 00:00:00

  • Functional organization of the autotransporter adhesin involved in diffuse adherence.

    abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01238-07

    authors: Charbonneau ME,Mourez M

    更新日期:2007-12-01 00:00:00

  • Mitochondrial translation products during release from glucose repression in Saccharomyces cerevisiae.

    abstract::Mitochondrial protein synthesis was studied during release from glucose repression in Saccharomyces cerevisiae cells bearing different mitochondrial genomes. The increase in the rate of the synthesis of mitochondrial translation products was analyzed during respiratory induction. Different kinetic patterns were found ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.3.1125-1132.1983

    authors: Falcone C,Agostinelli M,Frontali L

    更新日期:1983-03-01 00:00:00