Cj1386 is an ankyrin-containing protein involved in heme trafficking to catalase in Campylobacter jejuni.

Abstract:

:Campylobacter jejuni, a microaerophilic bacterium, is the most frequent cause of human bacterial gastroenteritis. C. jejuni is exposed to harmful reactive oxygen species (ROS) produced during its own normal metabolic processes and during infection from the host immune system and from host intestinal microbiota. These ROS will damage DNA and proteins and cause peroxidation of lipids. Consequently, identifying ROS defense mechanisms is important for understanding how Campylobacter survives this environmental stress during infection. Construction of a ΔCj1386 isogenic deletion mutant and phenotypic assays led to its discovery as a novel oxidative stress defense gene. The ΔCj1386 mutant has an increased sensitivity toward hydrogen peroxide. The Cj1386 gene is located directly downstream from katA (catalase) in the C. jejuni genome. A ΔkatAΔ Cj1386 double deletion mutant was constructed and exhibited a sensitivity to hydrogen peroxide similar to that seen in the ΔCj1386 and ΔkatA single deletion mutants. This observation suggests that Cj1386 may be involved in the same detoxification pathway as catalase. Despite identical KatA abundances, catalase activity assays showed that the ΔCj1386 mutant had a reduced catalase activity relative to that of wild-type C. jejuni. Heme quantification of KatA protein from the ΔCj1386 mutant revealed a significant decrease in heme concentration. This indicates an important role for Cj1386 in heme trafficking to KatA within C. jejuni. Interestingly, the ΔCj1386 mutant had a reduced ability to colonize the ceca of chicks and was outcompeted by the wild-type strain for colonization of the gastrointestinal tract of neonate piglets. These results indicate an important role for Cj1386 in Campylobacter colonization and pathogenesis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Flint A,Sun YQ,Stintzi A

doi

10.1128/JB.05740-11

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

334-45

issue

2

eissn

0021-9193

issn

1098-5530

pii

JB.05740-11

journal_volume

194

pub_type

杂志文章
  • Bacteriochlorophyll and heme synthesis in Rhodopseudomonas spheroides: possible role of heme in regulation of the branched biosynthetic pathway.

    abstract::Synthesis of heme, measured by incorporation of iron-59, and of bacteriochlorophyll was studied with wild-type and mutant strains of Rhodopseudomonas spheroides. The wild type formed heme from glycine and succinate at one-fortieth the rate of bacteriochlorophyll under anaerobic-light conditions. Added delta-aminolevul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.712-720.1969

    authors: Lascelles J,Hatch TP

    更新日期:1969-05-01 00:00:00

  • Growth of the fission yeast, Schizosaccharomyces pombe, with late, eccentric, lytic fission in an unbalanced medium.

    abstract::Growth of Schizosaccharomyces pombe in batch cultures in a commercial defined medium was found to be normal until the last generation before the stationary phase. Approximately one-half the progeny of the last division lysed. Lysis occurred at breaks in the cell wall at cell-plates whose fission was eccentric. Normal ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.192-195.1967

    authors: Johnson BF

    更新日期:1967-07-01 00:00:00

  • Construction and analysis of hybrid Escherichia coli-Bacillus subtilis dnaK genes.

    abstract::The highly conserved DnaK chaperones consist of an N-terminal ATPase domain, a central substrate-binding domain, and a C-terminal domain whose function is not known. Since Bacillus subtilis dnaK was not able to complement an Escherichia coli dnaK null mutant, we performed domain element swap experiments to identify th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.6.1971-1974.1999

    authors: Mogk A,Bukau B,Lutz R,Schumann W

    更新日期:1999-03-01 00:00:00

  • Resistance of Salmonella typhimurium mutants to galactose death.

    abstract::A class of galactose-resistant mutants has been derived from strains of Salmonella typhimurium which are defective in uridine diphosphoglucose-4-epimerase. Resistant strains are phenotypically similar to parent organisms but do not lyse in the presence of galactose. Low levels of functional epimerase can be detected i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.103-109.1969

    authors: Shuster CW,Rundell K

    更新日期:1969-10-01 00:00:00

  • Oncoupling of protein and ribonucleic acid synthesis by 5',5',5'-trifluoroleucine in Salmonella typhimurium.

    abstract::The addition of 5',5',5'-trifluoroleucine (fluoroleucine) to leucine auxotrophs of Salmonella typhimurium permitted protein but not ribonucleic acid (RNA) synthesis to continue after leucine depletion. The uncoupling of the formation of these macromolecules by fluoroleucine was apparent if RNA and protein synthesis wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.1.107-112.1969

    authors: Trela JM,Freundlich M

    更新日期:1969-07-01 00:00:00

  • Morphological study of the reversion to bacillary form, of Bacillus megaterium protoplasts.

    abstract::Protoplasts of Bacillus megaterium readily reverted to bacillary form in liquid media and when plated in a soft-agar layer onto the surface of appropriate agar media. Three phases of the reversion sequence could be differentiated by phase contrast microscopy: (i) increase in size of the individual protoplasts, (ii) no...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.3.1172-1179.1976

    authors: Hadlaczky G,Fodor K,Alföldi L

    更新日期:1976-03-01 00:00:00

  • Mechanism of acetate synthesis from CO2 by Clostridium acidiurici.

    abstract::Total synthesis of acetate from CO2 by Clostridium acidiurici during fermentations of hypoxanthine has been shown to involve synthesis of glycine from methylenetetrahydrofolate, CO2, and NH3. The glycine is converted to serine by the addition of methylenetetrahydrofolate, and the resulting serine is converted to pyruv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.468-478.1979

    authors: Waber LJ,Wood HG

    更新日期:1979-11-01 00:00:00

  • Strain-Dependent RstA Regulation of Clostridioides difficile Toxin Production and Sporulation.

    abstract::The anaerobic spore former Clostridioides difficile causes significant diarrheal disease in humans and other mammals. Infection begins with the ingestion of dormant spores, which subsequently germinate within the host gastrointestinal tract. There, the vegetative cells proliferate and secrete two exotoxins, TcdA and T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00586-19

    authors: Edwards AN,Krall EG,McBride SM

    更新日期:2020-01-02 00:00:00

  • The Moraxella catarrhalis immunoglobulin D-binding protein MID has conserved sequences and is regulated by a mechanism corresponding to phase variation.

    abstract::The prevalence of the Moraxella catarrhalis immunoglobulin D (IgD)-binding outer membrane protein MID and its gene was determined in 91 clinical isolates and in 7 culture collection strains. Eighty-four percent of the clinical Moraxella strains expressed MID-dependent IgD binding. The mid gene was detected in all stra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.7.2285-2295.2003

    authors: Möllenkvist A,Nordström T,Halldén C,Christensen JJ,Forsgren A,Riesbeck K

    更新日期:2003-04-01 00:00:00

  • Precursor in cotranslational secretion of diphtheria toxin.

    abstract::By extracellular labeling of peptides of intact Corynebacterium diphtheriae, followed by fractionation of the cells and chain completion by isolated polysomes, it is shown that diphtheria toxin is formed and secreted cotranslationally by membrane-bound polysomes; free polysomes from none. Moreover, when the chains on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.1.184-189.1980

    authors: Smith WP,Tai PC,Murphy JR,Davis BD

    更新日期:1980-01-01 00:00:00

  • Comparison of lipopolysaccharides from Agmenellum quadruplicatum to Escherichia coli and Salmonella typhimurium by using thin-layer chromatography.

    abstract::Lipopolysaccharide (LPS) was isolated from the unicellular blue-green bacterium Agmenellum quadruplicatum using the procedure of Westphal and Jann (1965). It was composed of a lipid A and polysaccharide region suggesting a similarity to other gram-negative LPSs. Chemical analyses demonstrated the presence of glucose, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1566-1573.1975

    authors: Buttke TM,Ingram LO

    更新日期:1975-12-01 00:00:00

  • Transcriptional regulation of the virA and virG genes of Agrobacterium tumefaciens.

    abstract::We have used transcriptional and translational fusions between various vir gene promoters and the lacZ gene to study the regulation of vir genes. Like other vir promoters, the virA promoter was induced by acetosyringone in a virA virG-dependent fashion. In addition to being induced by acetosyringone, the virG promoter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.4047-4054.1988

    authors: Winans SC,Kerstetter RA,Nester EW

    更新日期:1988-09-01 00:00:00

  • Regulation of metalloprotease gene expression in Vibrio vulnificus by a Vibrio harveyi LuxR homologue.

    abstract::Expression of the Vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when bacterial growth reached the late log phase. A similar pattern of expression has been found in the metalloprotease gene of Vibrio cholerae, and this has been shown to be regulated by a Vibrio harveyi LuxR-like transcriptional act...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.4.1369-1375.2001

    authors: Shao CP,Hor LI

    更新日期:2001-02-01 00:00:00

  • Molecular cloning and analysis of genes for production of K5, K7, K12, and K92 capsular polysaccharides in Escherichia coli.

    abstract::With a DNA fragment from within the region encoding the transport functions for K1 production as a hybridization probe in Southern blot experiments, homologous DNA sequences were detected in the DNA from Escherichia coli strains producing K5, K7, K92, and K100 capsular polysaccharides. No homology with the laboratory ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.3.1228-1233.1986

    authors: Roberts I,Mountford R,High N,Bitter-Suermann D,Jann K,Timmis K,Boulnois G

    更新日期:1986-12-01 00:00:00

  • Functional organization of the autotransporter adhesin involved in diffuse adherence.

    abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01238-07

    authors: Charbonneau ME,Mourez M

    更新日期:2007-12-01 00:00:00

  • Phosphatidylinositol, a phospholipid of ice-nucleating bacteria.

    abstract::The nature of the phospholipids of the various bacteria that have ice nucleation activity in supercooled water has been determined. The seven bacteria studied included Pseudomonas syringae, Erwinia herbicola, three Escherichia coli K-12 strains that are phenotypically Ice+ because they contain plasmids with different ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.6.2053-2060.1991

    authors: Kozloff LM,Turner MA,Arellano F,Lute M

    更新日期:1991-03-01 00:00:00

  • Metabolism of biphenyl by Aspergillus toxicarius: induction of hydroxylating activity and accumulation of water-soluble conjugates.

    abstract::Biphenyl metabolism in Aspergillus toxicarius occurs by successive hydroxylations in the 4- and 4'-positions, followed by conjugation with sulfate to produce 4-hydroxybiphenyl-O-sulfonic acid and 4,4'-dihydroxybiphenyl-O-sulfonic acid. The hydroxylation reactions normally occur only after a prolonged lag period after ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.1.49-57.1983

    authors: Golbeck JH,Albaugh SA,Radmer R

    更新日期:1983-10-01 00:00:00

  • Comparison of butyric type of fermentation in sporogenic and asporogenic mutants of Clostridium botulinum.

    abstract::Glucose-adapted cells of a sporogenic mutant. MSp(+), and an asporogenic mutant, RSpoIIIa, of Clostridium botulinum type E rapidly fermented glucose, fructose, maltose, and sucrose, resulting in cytoplasmic granulation, heavy growth, a pH of <6.0, and sporulation of the MSp(+) mutant ranging from 60 to 80%. In Tryptic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.1.74-80.1974

    authors: Emeruwa AC,Hawirko RZ,Halvorson H,Suzuki I

    更新日期:1974-10-01 00:00:00

  • Induction of colicin 1a at high temperature.

    abstract::Induction of colicin la at a high temperature (42 C) was demonstrated in a wild strain of Shigella sonnei. After transfer of the Col factor, a similar effect was observed in those recipient cells acquiring colicinogeny. The induction is suggested to be due to the presence of a thermosensitive colicin repressor. The wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.3.720-723.1971

    authors: Gromkova RH

    更新日期:1971-06-01 00:00:00

  • Proteus mirabilis MR/P fimbrial operon: genetic organization, nucleotide sequence, and conditions for expression.

    abstract::Proteus mirabilis, an agent of urinary tract infection, expresses at least four fimbrial types. Among these are the MR/P (mannose-resistant/Proteus-like) fimbriae. MrpA, the structural subunit, is optimally expressed at 37 degrees C in Luria broth cultured statically for 48 h by each of seven strains examined. Genes e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.11.3412-3419.1994

    authors: Bahrani FK,Mobley HL

    更新日期:1994-06-01 00:00:00

  • Pilus production in Acinetobacter baumannii is growth phase dependent and essential for natural transformation.

    abstract::Acinetobacter baumannii is a severe threat to human health as a frequently multidrug-resistant hospital-acquired pathogen. Part of the danger from this bacterium comes from its genome plasticity and ability to evolve quickly by taking up and recombining external DNA into its own genome in a process called natural comp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00034-21

    authors: Vesel N,Blokesch M

    更新日期:2021-01-25 00:00:00

  • Genetic determinants of Silicibacter sp. TM1040 motility.

    abstract::Silicibacter sp. TM1040 is a member of the marine Roseobacter clade of Alphaproteobacteria that forms symbioses with unicellular eukaryotic phytoplankton, such as dinoflagellates. The symbiosis is complex and involves a series of steps that physiologically change highly motile bacteria into cells that readily form bio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00429-09

    authors: Belas R,Horikawa E,Aizawa S,Suvanasuthi R

    更新日期:2009-07-01 00:00:00

  • The FtsH protein accumulates at the septum of Bacillus subtilis during cell division and sporulation.

    abstract::The ftsH gene encodes an ATP- and Zn(2+)-dependent metalloprotease which is anchored to the cytoplasmic membrane via two transmembrane segments in such a way that the very short amino- and the long carboxy termini are exposed to the cytoplasm. Deletion of the ftsH gene in Bacillus subtilis results in a pleiotropic phe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.13.3870-3873.2000

    authors: Wehrl W,Niederweis M,Schumann W

    更新日期:2000-07-01 00:00:00

  • A 54-kilodalton protein encoded by pBtoxis is required for parasporal body structural integrity in Bacillus thuringiensis subsp. israelensis.

    abstract::Strains of Bacillus thuringiensis such as B. thuringiensis subsp. israelensis (ONR-60A) and B. thuringiensis subsp. morrisoni (PG-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06095-11

    authors: Diaz-Mendoza M,Bideshi DK,Federici BA

    更新日期:2012-03-01 00:00:00

  • Cell shape and division in Escherichia coli: experiments with shape and division mutants.

    abstract::Double mutants which carry mutations in genes (rodA, pbpA) required for cell elongation (i.e., maintenance of rod shape) in combination with mutations in genes (ftsA, ftsI, ftsQ, or ftsZ) required for septation were constructed. Such mutants were able to grow for about two mass doublings at a normal rate at the restri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.2.615-622.1985

    authors: Begg KJ,Donachie WD

    更新日期:1985-08-01 00:00:00

  • Rhodobacter sphaeroides WS8 expresses a polypeptide that is similar to MotB of Escherichia coli.

    abstract::A gene which complements a paralyzed flagellar mutant of Rhodobacter sphaeroides was sequenced. The derived protein sequence has similarity to MotB. R. sphaeroides MotB lacks the C-terminal peptidoglycan-binding motif of other MotB proteins. This divergence of sequence may reflect the unusual, unidirectional, stop-sta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.10.2929-2932.1995

    authors: Shah DS,Armitage JP,Sockett RE

    更新日期:1995-05-01 00:00:00

  • Detection and characterization of conjugative degradative plasmids in xenobiotic-degrading Sphingomonas strains.

    abstract::A systematic survey for the presence of plasmids in 17 different xenobiotic-degrading Sphingomonas strains was performed. In almost all analyzed strains, two to five plasmids with sizes of about 50 to 500 kb were detected by using pulsed-field gel electrophoresis. A comparison of plasmid preparations untreated or trea...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.12.3862-3872.2004

    authors: Basta T,Keck A,Klein J,Stolz A

    更新日期:2004-06-01 00:00:00

  • Proteus mirabilis mutants defective in swarmer cell differentiation and multicellular behavior.

    abstract::Proteus mirabilis is a dimorphic bacterium which exists in liquid cultures as a 1.5- to 2.0-microns motile swimmer cell possessing 6 to 10 peritrichous flagella. When swimmer cells are placed on a surface, they differentiate by a combination of events that ultimately produce a swarmer cell. Unlike the swimmer cell, th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.6279-6288.1991

    authors: Belas R,Erskine D,Flaherty D

    更新日期:1991-10-01 00:00:00

  • Role and specificity of plasmid RP4-encoded DNA primase in bacterial conjugation.

    abstract::The role of the DNA primase of IncP plasmids was examined with a derivative of RP4 containing Tn7 in the primase gene (pri). The mutant was defective in mediating bacterial conjugation, with the deficiency varying according to the bacterial strains used as donors and recipients. Complementation tests involving recombi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.1.12-17.1986

    authors: Merryweather A,Barth PT,Wilkins BM

    更新日期:1986-07-01 00:00:00

  • AmpC and AmpH, proteins related to the class C beta-lactamases, bind penicillin and contribute to the normal morphology of Escherichia coli.

    abstract::Two proteins that bind penicillin were observed in Escherichia coli infected with lambda phages 141, 142, 650, and 651 from the Kohara genomic library. These phages carry chromosomal DNA fragments that do not contain any known penicillin binding protein (PBP) genes, indicating that unrecognized gene products were exhi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.19.6112-6121.1997

    authors: Henderson TA,Young KD,Denome SA,Elf PK

    更新日期:1997-10-01 00:00:00