Comparison of butyric type of fermentation in sporogenic and asporogenic mutants of Clostridium botulinum.

abstract：:Binding sites for the Escherichia coli protein integration host factor (IHF) include a set of conserved bases that can be summarized by the consensus sequence WATCAANNNNTTR (W is dA or dT, R is dA or dG, and N is any nucleotide). However, additional 5'-proximal bases, whose common feature is a high dA+dT content, are ...

journal_title：Journal of bacteriology

authors： Hales LM,Gumport RI,Gardner JF

更新日期：1994-05-01 00:00:00

abstract：:A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

journal_title：Journal of bacteriology

authors： Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

更新日期：1995-07-01 00:00:00

abstract：:We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1 and PUT2, also required a functional GLN3 protein. In addition, GLN3 protein was required for expression of the DAL1, DA...

journal_title：Journal of bacteriology

authors： Daugherty JR,Rai R,el Berry HM,Cooper TG

更新日期：1993-01-01 00:00:00

abstract：:By use of Arthrobacter globiformis SI55, a psychrotrophic bacterium capable of growth between -5 and +32 degrees C, we cloned and sequenced capA, a gene homologous to cspA encoding the major cold shock protein in Escherichia coli. The deduced protein sequence has a high level of identity with the sequences of other Cs...

journal_title：Journal of bacteriology

authors： Berger F,Normand P,Potier P

更新日期：1997-09-01 00:00:00

abstract：:The isolation of a Streptococcus thermophilus CNRZ368 mutant displaying a long-chain phenotype allowed us to identify the cse gene (for cellular segregation). The N terminus of Cse exhibits high similarity to Streptococcus agalactiae surface immunogenic protein (SIP), while its C terminus exhibits high similarity to S...

journal_title：Journal of bacteriology

authors： Borges F,Layec S,Thibessard A,Fernandez A,Gintz B,Hols P,Decaris B,Leblond-Bourget N

更新日期：2005-04-01 00:00:00

abstract：:Fusidic acid or chloramphenicol was used to inhibit peptide synthesis to 1% of normal in Escherichia coli B, strain AS19. After 10 min of inhibition, peptide synthesis could be quickly restored to 80% of the normal rate after washing the bacteria on a filter. However, even in the presence of adenosine 3'-5'-cyclic-mon...

journal_title：Journal of bacteriology

authors： Pastushok C,Kennell D

更新日期：1974-02-01 00:00:00

abstract：:A liquid chromatography-hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry approach was used to determine the differential abundance of proteins in acetate-grown cells compared to that of proteins in methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically lab...

journal_title：Journal of bacteriology

authors： Li Q,Li L,Rejtar T,Lessner DJ,Karger BL,Ferry JG

更新日期：2006-01-01 00:00:00

abstract：:Under secondary metabolic conditions, the lignin-degrading basidiomycete Phanerochaete chrysosporium mineralizes 2,4, 6-trichlorophenol. The pathway for the degradation of 2,4, 6-trichlorophenol has been elucidated by the characterization of fungal metabolites and oxidation products generated by purified lignin peroxi...

journal_title：Journal of bacteriology

authors： Reddy GV,Gelpke MD,Gold MH

更新日期：1998-10-01 00:00:00

abstract：:The number and disposition of flagella harbored by eubacteria are regulated by a specific trait successfully maintained over generations. The genes governing the number of flagella in Bacillus subtilis have never been identified, although the ifm locus has long been recognized to influence the motility phenotype of th...

journal_title：Journal of bacteriology

authors： Calvio C,Celandroni F,Ghelardi E,Amati G,Salvetti S,Ceciliani F,Galizzi A,Senesi S

更新日期：2005-08-01 00:00:00

abstract：:Cell walls of 19 Micromonospora species were analyzed for their components. All the cell walls had xylose and arabinose, but the presence of glucose, galactose, mannose, or rhamnose depended on the strain. Amino acids present in the walls consisted of glycine, glutamic acid, diaminopimelic acid, and alanine, in a mola...

journal_title：Journal of bacteriology

authors： Kawamoto I,Oka T,Nara T

更新日期：1981-05-01 00:00:00

abstract：:The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functiona...

journal_title：Journal of bacteriology

authors： Hennig S,Ziebuhr W

更新日期：2008-02-01 00:00:00

abstract：:When an Escherichia coli K-12 culture was starved for glucose, 50% of the cells lost viability in about 6 days. When a K-12 mutant lacking five distinct peptidase activities, CM89, was starved in the same manner, viability was lost much more rapidly; 50% of the cells lost viability in about 2 days, whereas a parent st...

journal_title：Journal of bacteriology

authors： Reeve CA,Bockman AT,Matin A

更新日期：1984-03-01 00:00:00

abstract：:The cell wall constituents of Leuconostoc citrovorum 8082, L. mesenteroides 10830a, and L. mesenteroides 11449 have been ascertained. All three strains contained glycerol. Glucose and rhamnose were the major reducing sugar constituents. Alanine, glutamic acid, lysine, glucosamine, and muramic acid were the principal a...

journal_title：Journal of bacteriology

authors： Harney SJ,Simopoulos ND,Ikawa M

更新日期：1967-01-01 00:00:00

abstract：:Shiga toxin (STX), a bacterial toxin produced by Shigella dysenteriae type 1, is a hexamer composed of five receptor-binding B subunits which encircle an alpha-helix at the carboxyl terminus of the enzymatic A polypeptide. Hybrid toxins constructed by fusing the A polypeptide sequences of STX and Shiga-like toxin type...

journal_title：Journal of bacteriology

authors： Jemal C,Haddad JE,Begum D,Jackson MP

更新日期：1995-06-01 00:00:00

abstract：:This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...

journal_title：Journal of bacteriology

authors： Harley JB,Santangelo GM,Rasmussen H,Goldfine H

更新日期：1978-06-01 00:00:00

abstract：:Spiralin could not be solubilized in the absence of detergents, and it was shown by charge-shift crossed immunoelectrophoresis that this protein was capable of binding detergents under nondenaturing conditions. These properties indicate the amphiphilic nature of spiralin, which therefore should be regarded as an intri...

journal_title：Journal of bacteriology

authors： Wróblewski H

更新日期：1979-11-01 00:00:00

abstract：:Clostridium perfringens causes gas gangrene and gastrointestinal (GI) diseases in humans. The most common cause of C. perfringens-associated food poisoning is the consumption of C. perfringens vegetative cells followed by sporulation and production of enterotoxin in the gut. Despite the importance of spore formation i...

journal_title：Journal of bacteriology

authors： Ohtani K,Hirakawa H,Paredes-Sabja D,Tashiro K,Kuhara S,Sarker MR,Shimizu T

更新日期：2013-06-01 00:00:00

abstract：:We cloned and sequenced a fragment of the Bacillus stearothermophilus NUB36 chromosome that contains two open reading frames (ORFs) whose products were detected only in cells of cultures grown in complex medium at high temperature. The nucleotide sequence of the two ORFs exhibited significant identity to the sequence ...

journal_title：Journal of bacteriology

authors： Wu L,Welker NE

更新日期：1991-08-01 00:00:00

abstract：:The rcs phosphorelay pathway components were originally identified as regulators of capsule synthesis. In addition to the transmembrane sensor kinase RcsC, the RcsA coregulator, and the response regulator RcsB, two new components have been characterized, RcsD and RcsF. RcsD, the product of the yojN gene, now renamed r...

journal_title：Journal of bacteriology

authors： Majdalani N,Heck M,Stout V,Gottesman S

更新日期：2005-10-01 00:00:00

abstract：:The metE gene of Salmonella typhimurium was cloned into plasmid pACYC184 by using a lambda gt7 -metE transducing phage as a source of metE DNA. The recombinant plasmid, designated pGS41 , carries a 12.8-kilobase-pair EcoRI insert fragment. The metE gene was subcloned from pGS41 into plasmid pBR322 on a 4.2-kilobase-pa...

journal_title：Journal of bacteriology

authors： Schulte LL,Stauffer LT,Stauffer GV

更新日期：1984-06-01 00:00:00

abstract：:Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...

journal_title：Journal of bacteriology

authors： Rushing BG,Long SR

更新日期：1995-12-01 00:00:00

abstract：:A novel tungstate and molybdate binding protein has been discovered from the hyperthermophilic archaeon Pyrococcus furiosus. This tungstate transport protein A (WtpA) is part of a new ABC transporter system selective for tungstate and molybdate. WtpA has very low sequence similarity with the earlier-characterized tran...

journal_title：Journal of bacteriology

authors： Bevers LE,Hagedoorn PL,Krijger GC,Hagen WR

更新日期：2006-09-01 00:00:00

abstract：:To examine the role of the amino acid residues (between positions 258 and 275 and positions 297 and 298) of the alpha-subunit of RNA polymerase in TyrR-mediated activation of the mtr promoter, we have carried out in vitro transcription experiments using a set of mutant RNA polymerases with a supercoiled mtr template. ...

journal_title：Journal of bacteriology

authors： Yang J,Murakami K,Camakaris H,Fujita N,Ishihama A,Pittard AJ

更新日期：1997-10-01 00:00:00

abstract：:Thirteen different serotypes of Listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. Although the known virulence genes are present in all serotypes, greater than 90% of human cases of listeriosis are caused by serotypes 1/2a, 1/2b, and 4b and nearly all outbreaks of...

journal_title：Journal of bacteriology

authors： Zhang C,Zhang M,Ju J,Nietfeldt J,Wise J,Terry PM,Olson M,Kachman SD,Wiedmann M,Samadpour M,Benson AK

更新日期：2003-09-01 00:00:00

abstract：:The plasmid ColE2-P9 Rep protein specifically binds to the cognate replication origin to initiate DNA replication. The replicons of the plasmids ColE2-P9 and ColE3-CA38 are closely related, although the actions of the Rep proteins on the origins are specific to the plasmids. The previous chimera analysis identified tw...

journal_title：Journal of bacteriology

authors： Aoki K,Shinohara M,Itoh T

更新日期：2007-03-01 00:00:00

abstract：:Using nitrogen-fixing Sinorhizobium species that interact with Medicago plants as a model system, we aimed at clarifying how sex has shaped the diversity of bacteria associated with the genus Medicago on the interspecific and intraspecific scales. To gain insights into the diversification of these symbionts, we inferr...

journal_title：Journal of bacteriology

authors： Bailly X,Olivieri I,Brunel B,Cleyet-Marel JC,Béna G

更新日期：2007-07-01 00:00:00

abstract：:Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was fo...

journal_title：Journal of bacteriology

authors： Markus Z,Silverman GJ

更新日期：1969-02-01 00:00:00

abstract：:The gene for the catabolic NAD-linked glutamate dehydrogenase of Peptostreptococcus asaccharolyticus was cloned by selection of Escherichia coli for complementation of a biosynthetic defect. Cloned fragments containing the gene and the P. asaccharolyticus transcription and translation signals are very highly expressed...

journal_title：Journal of bacteriology

authors： Snedecor B,Chu H,Chen E

更新日期：1991-10-01 00:00:00

abstract：:The major cotransduction gap of the Escherichia coli chromosome extends from mini 31 to 34. We have inserted transposons through this gap which, by sequential transduction, link sbcA (min 29.8) with manA (min 35.7) and thus eliminate the gap. These results indicate that the length of DNA in the region, as measured by ...

journal_title：Journal of bacteriology

authors： Fouts KE,Barbour SD

更新日期：1982-01-01 00:00:00

abstract：:Mutational analysis of the carboxy-terminal region of Escherichia coli HlyC was performed by site-directed mutagenesis. Replacement of residue Val-127 or Lys-129 reduced the activity of HlyC to about 30 or 60%, respectively, of that of the wild type, while replacement of Gly-128 reduced the activity to less than 1% of...

journal_title：Journal of bacteriology

authors： Guzmán-Verri C,García F,Arvidson S

更新日期：1997-09-01 00:00:00