Electron transport in the pathway of acetate conversion to methane in the marine archaeon Methanosarcina acetivorans.

Abstract:

:A liquid chromatography-hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry approach was used to determine the differential abundance of proteins in acetate-grown cells compared to that of proteins in methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically labeled with 14N versus 15N. The 246 differentially abundant proteins in M. acetivorans were compared with the previously reported 240 differentially expressed genes of the freshwater isolate Methanosarcina mazei determined by transcriptional profiling of acetate-grown cells compared to methanol-grown cells. Profound differences were revealed for proteins involved in electron transport and energy conservation. Compared to methanol-grown cells, acetate-grown M. acetivorans synthesized greater amounts of subunits encoded in an eight-gene transcriptional unit homologous to operons encoding the ion-translocating Rnf electron transport complex previously characterized from the Bacteria domain. Combined with sequence and physiological analyses, these results suggest that M. acetivorans replaces the H2-evolving Ech hydrogenase complex of freshwater Methanosarcina species with the Rnf complex, which generates a transmembrane ion gradient for ATP synthesis. Compared to methanol-grown cells, acetate-grown M. acetivorans synthesized a greater abundance of proteins encoded in a seven-gene transcriptional unit annotated for the Mrp complex previously reported to function as a sodium/proton antiporter in the Bacteria domain. The differences reported here between M. acetivorans and M. mazei can be attributed to an adaptation of M. acetivorans to the marine environment.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Li Q,Li L,Rejtar T,Lessner DJ,Karger BL,Ferry JG

doi

10.1128/JB.188.2.702-710.2006

keywords:

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

702-10

issue

2

eissn

0021-9193

issn

1098-5530

pii

188/2/702

journal_volume

188

pub_type

杂志文章
  • Regulation of arginase activity by intermediates of the arginine biosynthetic pathway in Neurospora crassa.

    abstract::It has been found that, in Neurospora crassa, arginine synthesized from exogenous citrulline was not as effectively hydrolyzed as exogenous arginine. This was explained by the observed inhibition of arginase in vitro and in vivo by citrulline. The high arginine pool formed from exogenous citrulline feedback inhibits t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.3.870-877.1972

    authors: Mora J,Salceda R,Sanchez S

    更新日期:1972-06-01 00:00:00

  • Immunochemistry of the cell walls of Listeria monocytogenes.

    abstract::The antigenic specificity of Listeria monocytogenes types I, II, III, IVa, and IVb was studied by immunochemical techniques. Immunologically active carbohydrates of the various types were extracted from cell walls and were chemically analyzed. Types I and II contained predominantly glucosamine and rhamnose; type III, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.486-493.1969

    authors: Ullmann WW,Cameron JA

    更新日期:1969-05-01 00:00:00

  • Molecular cloning of the uhp region and evidence for a positive activator for expression of the hexose phosphate transport system of Escherichia coli.

    abstract::The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1062-1070.1983

    authors: Shattuck-Eidens DM,Kadner RJ

    更新日期:1983-09-01 00:00:00

  • Characterization of the genes and proteins of a two-component system from the hyperthermophilic bacterium Thermotoga maritima.

    abstract::As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5579-5585.1996

    authors: Lee PJ,Stock AM

    更新日期:1996-10-01 00:00:00

  • The Conserved Tetratricopeptide Repeat-Containing C-Terminal Domain of Pseudomonas aeruginosa FimV Is Required for Its Cyclic AMP-Dependent and -Independent Functions.

    abstract:UNLABELLED:FimV is a Pseudomonas aeruginosa inner membrane protein that regulates intracellular cyclic AMP (cAMP) levels-and thus type IV pilus (T4P)-mediated twitching motility and type II secretion (T2S)-by activating the adenylate cyclase CyaB. Its cytoplasmic domain contains three predicted tetratricopeptide repeat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00322-16

    authors: Buensuceso RN,Nguyen Y,Zhang K,Daniel-Ivad M,Sugiman-Marangos SN,Fleetwood AD,Zhulin IB,Junop MS,Howell PL,Burrows LL

    更新日期:2016-07-28 00:00:00

  • Coordinate regulation of the Escherichia coli formate dehydrogenase fdnGHI and fdhF genes in response to nitrate, nitrite, and formate: roles for NarL and NarP.

    abstract::Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons. To examine how two of the formate dehyrogenase operons (fdnGHI and fdhF) are expressed anaerobically in the presence of low, intermediate, and high levels of nitrate, nitrite, and formate, chemostat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.17.5076-5085.2003

    authors: Wang H,Gunsalus RP

    更新日期:2003-09-01 00:00:00

  • Homology of ribosomal ribonucleic acid of Desulfovibrio species with Desulfovibrio vulgaris.

    abstract::Three species of Desulfovibrio were found to have a high degree of ribosomal ribonucleic acid homology with Desulfovibrio vulgaris. Desulfotomaculum nigrificans, which is also a sulfate-reducing anaerobe, had only 38% ribosomal ribonucleic acid homology with D. vulgaris. The homologies of six other unrelated genera we...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.3.717-719.1971

    authors: Pace B,Campbell LL

    更新日期:1971-06-01 00:00:00

  • Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system.

    abstract::Quie, Paul G. (University of Minnesota, Minneapolis) and Lewis W. Wannamaker. Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system. J. Bacteriol. 82:770-783. 1961.-The staphylococcal factor that produces particulate proteolysis (Muller phenomenon) in whole blood or hemoglobinserum agar plat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.5.770-783.1961

    authors: QUIE PG,WANNAMAKER LW

    更新日期:1961-11-01 00:00:00

  • Purification and characterization of a cell-associated, soluble mannanase from Bacteroides ovatus.

    abstract::Bacteroides ovatus, a human colonic anaerobe, utilizes the galactomannan guar gum as a sole source of carbohydrate. Previously, we found that none of the galactomannan-degrading enzymes were extracellular, and we characterized an outer membrane mannanase which hydrolyzes the backbone of guar gum to produce large fragm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.2038-2043.1987

    authors: Gherardini FC,Salyers AA

    更新日期:1987-05-01 00:00:00

  • Differential recognition of surface proteins in Streptococcus pyogenes by two sortase gene homologs.

    abstract::The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.8.2181-2191.2002

    authors: Barnett TC,Scott JR

    更新日期:2002-04-01 00:00:00

  • Importance of the position of TYR R boxes for repression and activation of the tyrP and aroF genes in Escherichia coli.

    abstract::Tyrosine-mediated repression of aroF and tyrP was studied by inserting DNA sequences between the two adjacent TYR R boxes which, in each case, overlap the respective RNA polymerase binding sites of these genes. In both cases, repression was greatest when homologous regions of these two TYR R boxes were on the same fac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.16.5079-5085.1991

    authors: Andrews AE,Dickson B,Lawley B,Cobbett C,Pittard AJ

    更新日期:1991-08-01 00:00:00

  • Heterogenetic antigens of gram-positive bacteria.

    abstract::Chorpenning, Frank W. (The Ohio State University, Columbus), and Matthew C. Dodd. Heterogenetic antigens of gram-positive bacteria. J. Bacteriol. 91:1440-1445. 1966.-Soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune ra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.4.1440-1445.1966

    authors: Chorpenning FW,Dodd MC

    更新日期:1966-04-01 00:00:00

  • Mutations in a new Streptomyces coelicolor locus which globally block antibiotic biosynthesis but not sporulation.

    abstract::Streptomyces coelicolor produces four known antibiotics. To define genetic elements that regulate antibiotic synthesis, we screened for mutations that visibly blocked synthesis of the two pigmented antibiotics and found that the mutant strains which we recovered were of two classes--double mutants and mutants in which...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.2962-2969.1990

    authors: Adamidis T,Riggle P,Champness W

    更新日期:1990-06-01 00:00:00

  • Genome-wide analyses in bacteria show small-RNA enrichment for long and conserved intergenic regions.

    abstract::Interest in finding small RNAs (sRNAs) in bacteria has significantly increased in recent years due to their regulatory functions. Development of high-throughput methods and more sophisticated computational algorithms has allowed rapid identification of sRNA candidates in different species. However, given their various...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02359-14

    authors: Tsai CH,Liao R,Chou B,Palumbo M,Contreras LM

    更新日期:2015-01-01 00:00:00

  • Function of a chemotaxis-like signal transduction pathway in modulating motility, cell clumping, and cell length in the alphaproteobacterium Azospirillum brasilense.

    abstract::A chemotaxis signal transduction pathway (hereafter called Che1) has been previously identified in the alphaproteobacterium Azospirillum brasilense. Previous experiments have demonstrated that although mutants lacking CheB and/or CheR homologs from this pathway are defective in chemotaxis, a mutant in which the entire...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00734-08

    authors: Bible AN,Stephens BB,Ortega DR,Xie Z,Alexandre G

    更新日期:2008-10-01 00:00:00

  • The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology.

    abstract::Electron cryotomography was used to analyze the structure of the Lyme disease spirochete, Borrelia burgdorferi. This methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical fixing, dehydration, and staining steps of conventional electron microscopy. Using electron cr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01288-08

    authors: Charon NW,Goldstein SF,Marko M,Hsieh C,Gebhardt LL,Motaleb MA,Wolgemuth CW,Limberger RJ,Rowe N

    更新日期:2009-01-01 00:00:00

  • Role of Na+ cycle in cell volume regulation of Mycoplasma gallisepticum.

    abstract::The mechanism for the extrusion of Na+ from Mycoplasma gallisepticum cells was examined. Na+ efflux from cells was studied by diluting 22Na+-loaded cells into an isoosmotic NaCl solution and measuring the residual 22Na+ in the cells. Uphill 22Na+ efflux was found to be glucose dependent and linear with time over a 60-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.8.4410-4416.1989

    authors: Shirvan MH,Schuldiner S,Rottem S

    更新日期:1989-08-01 00:00:00

  • Purification and characterization of an inorganic pyrophosphatase from the extreme thermophile Thermus aquaticus.

    abstract::An inorganic pyrophosphatase was purified over 600-fold to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme is a tetramer of Mr = 84,000, has a sedimentation coefficient of 5.8S, a Stokes radius of 3.5 nm, and an isoelectric point of 5.7. Like the enzyme of Escherichia coli, the pyrophosphatase ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.1.318-321.1986

    authors: Verhoeven JA,Schenck KM,Meyer RR,Trela JM

    更新日期:1986-10-01 00:00:00

  • A Sorangium cellulosum (myxobacterium) gene cluster for the biosynthesis of the macrolide antibiotic soraphen A: cloning, characterization, and homology to polyketide synthase genes from actinomycetes.

    abstract::A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.13.3673-3679.1995

    authors: Schupp T,Toupet C,Cluzel B,Neff S,Hill S,Beck JJ,Ligon JM

    更新日期:1995-07-01 00:00:00

  • Mutation of waaC, encoding heptosyltransferase I in Campylobacter jejuni 81-176, affects the structure of both lipooligosaccharide and capsular carbohydrate.

    abstract::Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.9.3273-3279.2006

    authors: Kanipes MI,Papp-Szabo E,Guerry P,Monteiro MA

    更新日期:2006-05-01 00:00:00

  • Ultrastructure of Treponema microdentium and Borrelia vincentii.

    abstract::Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.5.1180-1191.1964

    authors: Bladen HA,Hampp EG

    更新日期:1964-05-01 00:00:00

  • PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA.

    abstract::Pseudomonas aeruginosa type IV pili (T4P) are virulence factors that promote infection of cystic fibrosis and immunosuppressed patients. As the absence of T4P impairs colonization, they are attractive targets for the development of novel therapeutics. Genes in the pilMNOPQ operon are important for both T4P assembly an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00032-13

    authors: Tammam S,Sampaleanu LM,Koo J,Manoharan K,Daubaras M,Burrows LL,Howell PL

    更新日期:2013-05-01 00:00:00

  • RELATIONSHIP BETWEEN TOXIGENICITY AND SPORULATING POTENCY OF CLOSTRIDIUM NOVYI.

    abstract::Nishida, Shoki (Kanazawa University, Kanazawa, Japan), and Gizo Nakagawara. Relationship between toxigenicity and sporulating potency of Clostridium novyi. J. Bacteriol. 89:993-995. 1965.-The less toxigenic the strains, the stronger was the sporulating potency of Clostridium novyi strains isolated. This was confirmed ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.4.993-995.1965

    authors: NISHIDA S,NAKAGAWARA G

    更新日期:1965-04-01 00:00:00

  • Specific inhibition of mature fungal serine proteinases and metalloproteinases by their propeptides.

    abstract::The function of the long propeptides of fungal proteinases is not known. Aspergillus fumigatus produces a 33-kDa serine proteinase of the subtilisin family and a 42-kDa metalloproteinase of the thermolysin family. These extracellular enzymes are synthesized as preproenzymes containing large amino-terminal propeptides....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.8.2211-2215.1996

    authors: Markaryan A,Lee JD,Sirakova TD,Kolattukudy PE

    更新日期:1996-04-01 00:00:00

  • Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli.

    abstract::DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA helicase II. Deletion muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2569-2575.1991

    authors: Washburn BK,Kushner SR

    更新日期:1991-04-01 00:00:00

  • Regulation of the potassium to sodium ratio and of the osmotic potential in relation to salt tolerance in yeasts.

    abstract::By using the isotope pairs (22)Na-(24)Na and (42)K-(86)Rb, the uptake and retention of Na and K was studied in the salt-tolerant Debaryomyces hansenii and in the less tolerant Saccharomyces cerevisiae at NaCl levels of 4 mm and 0.68, 1.35, and 2.7 m in the medium. The ratio of K to Na is much higher in the cells than ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.836-845.1969

    authors: Norkrans B,Kylin A

    更新日期:1969-11-01 00:00:00

  • Horizontal transference of S-layer genes within Thermus thermophilus.

    abstract::The S-layers of Thermus thermophilus HB27 and T. thermophilus HB8 are composed of protein units of 95 kDa (P95) and 100 kDa (P100), respectively. We have selected S-layer deletion mutants from both strains by complete replacement of the slpA gene. Mutants of the two strains showed similar defects in growth and morphol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.19.5460-5466.1995

    authors: Fernández-Herrero LA,Olabarría G,Castón JR,Lasa I,Berenguer J

    更新日期:1995-10-01 00:00:00

  • Sequence of b cytochromes relative to ubiquinone in the electron transport chain of Escherichia coli.

    abstract::A ubiquinone-deficient mutant, carrying mutations in two genes affecting ubiquinone biosynthesis, has been used, in comparison with a normal strain, to determine the sequence of some of the components of the electron transport chain of Escherichia coli. The amounts of cytochromes reduced during aerobic steady-state co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.477-484.1978

    authors: Downie JA,Cox GB

    更新日期:1978-02-01 00:00:00

  • Bacillus subtilis gene cluster involved in calcium carbonate biomineralization.

    abstract::Calcium carbonate precipitation, a widespread phenomenon among bacteria, has been investigated due to its wide range of scientific and technological implications. Nevertheless, little is known of the molecular mechanisms by which bacteria foster calcium carbonate mineralization. In our laboratory, we are studying calc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01450-06

    authors: Barabesi C,Galizzi A,Mastromei G,Rossi M,Tamburini E,Perito B

    更新日期:2007-01-01 00:00:00

  • Uptake of the glucose analogue 2-deoxyglucose by germinating mitospores of Allomyces macrogynus.

    abstract::Mitospores or cysts of Allomyces macrogynus do not take up the glucose analogue 2-deoxyglucose. Uptake of 2-deoxyglucose by germlings begins at 25 min into germination, the start of the rhizoid stage, and increases in rate by approximately 50-fold until 100 min into germination. The rate remains constant from 100 to 2...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.2.843-848.1975

    authors: Burke DD

    更新日期:1975-11-01 00:00:00