Abstract:
:Calcium carbonate precipitation, a widespread phenomenon among bacteria, has been investigated due to its wide range of scientific and technological implications. Nevertheless, little is known of the molecular mechanisms by which bacteria foster calcium carbonate mineralization. In our laboratory, we are studying calcite formation by Bacillus subtilis, in order to identify genes involved in the biomineralization process. A previous screening of UV mutants and of more than one thousand mutants obtained from the European B. subtilis Functional Analysis project allowed us to isolate strains altered in the precipitation phenotype. Starting from these results, we focused our attention on a cluster of five genes (lcfA, ysiA, ysiB, etfB, and etfA) called the lcfA operon. By insertional mutagenesis, mutant strains carrying each of the five genes were produced. All of them, with the exception of the strain carrying the mutated lcfA operon, were unable to form calcite crystals. By placing transcription under IPTG (isopropyl-beta-d-thiogalactopyranoside) control, the last gene, etfA, was identified as essential for the precipitation process. To verify cotranscription in the lcfA operon, reverse transcription-PCR experiments were performed and overlapping retrocotranscripts were found comprising three adjacent genes. The genes have putative functions linked to fatty acid metabolism. A link between calcium precipitation and fatty acid metabolism is suggested.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Barabesi C,Galizzi A,Mastromei G,Rossi M,Tamburini E,Perito Bdoi
10.1128/JB.01450-06subject
Has Abstractpub_date
2007-01-01 00:00:00pages
228-35issue
1eissn
0021-9193issn
1098-5530pii
JB.01450-06journal_volume
189pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/JB.100.1.103-109.1969
更新日期:1969-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1992-01-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.143.2.1090-1094.1980
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pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.108.3.1026-1033.1971
更新日期:1971-12-01 00:00:00
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更新日期:1982-08-01 00:00:00
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更新日期:1987-07-01 00:00:00
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更新日期:1988-05-01 00:00:00
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更新日期:1967-01-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-10-01 00:00:00