Abstract:
:To elucidate the biological functions of the ribosomal protein L34, which is encoded by the rpmH gene, the rpmH deletion mutant of Bacillus subtilis and two suppressor mutants were characterized. Although the ΔrpmH mutant exhibited a severe slow-growth phenotype, additional mutations in the yhdP or mgtE gene restored the growth rate of the ΔrpmH strain. Either the disruption of yhdP, which is thought to be involved in the efflux of Mg(2+), or overexpression of mgtE, which plays a major role in the import of Mg(2+), could suppress defects in both the formation of the 70S ribosome and growth caused by the absence of L34. Interestingly, the Mg(2+) content was lower in the ΔrpmH cells than in the wild type, and the Mg(2+) content in the ΔrpmH cells was restored by either the disruption of yhdP or overexpression of mgtE. In vitro experiments on subunit association demonstrated that 50S subunits that lacked L34 could form 70S ribosomes only at a high concentration of Mg(2+). These results showed that L34 is required for efficient 70S ribosome formation and that L34 function can be restored partially by Mg(2+). In addition, the Mg(2+) content was consistently lower in mutants that contained significantly reduced amounts of the 70S ribosome, such as the ΔrplA (L1) and ΔrplW (L23) strains and mutant strains with a reduced number of copies of the rrn operon. Thus, the results indicated that the cellular Mg(2+) content is influenced by the amount of 70S ribosomes.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Akanuma G,Kobayashi A,Suzuki S,Kawamura F,Shiwa Y,Watanabe S,Yoshikawa H,Hanai R,Ishizuka Mdoi
10.1128/JB.01896-14subject
Has Abstractpub_date
2014-11-01 00:00:00pages
3820-30issue
22eissn
0021-9193issn
1098-5530pii
JB.01896-14journal_volume
196pub_type
杂志文章abstract::The genome of Lactobacillus salivarius UCC118 includes a 242-kb megaplasmid, pMP118. We now show that 33 strains of L. salivarius isolated from humans and animals all harbor a megaplasmid, which hybridized with the repA and repE replication origin probes of pMP118. Linear megaplasmids that did not hybridize with the p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00447-07
更新日期:2007-09-01 00:00:00
abstract::Polyamines are present in high concentrations in archaea, yet little is known about their synthesis, except by extrapolation from bacterial and eucaryal systems. S-Adenosylmethionine (AdoMet) decarboxylase, a pyruvoyl group-containing enzyme that is required for spermidine biosynthesis, has been previously identified ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.23.6667-6672.2000
更新日期:2000-12-01 00:00:00
abstract::We have constructed a suicide vector, pU1800, containing the transposable element TnphoA (Tn5 IS50L::phoA), for the purpose of producing protein fusions in vivo between the Escherichia coli alkaline phosphatase (APase) and proteins of the facultative photoheterotroph, Rhodobacter sphaeroides. We introduced TnphoA into...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.8.4385-4394.1989
更新日期:1989-08-01 00:00:00
abstract::In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.375-382.1972
更新日期:1972-08-01 00:00:00
abstract::Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.23.6952-6957.1995
更新日期:1995-12-01 00:00:00
abstract::A nitrate-utilizing strain of marine bacteria was isolated in which luciferase was inducible by l-arginine. The induction was highly specific; structural analogues of arginine were ineffective, as were other natural amino acids. Several metabolites of arginine acted as weak inducers but did not affect the rate of indu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1638-1647.1967
更新日期:1967-11-01 00:00:00
abstract::In Salmonella enterica serovar Typhimurium, the stationary-phase sigma factor sigma(S) (RpoS) is required for virulence, stress resistance, biofilm formation, and development of the rdar morphotype. This morphotype is a multicellular behavior characterized by expression of the adhesive extracellular matrix components ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00033-06
更新日期:2006-06-01 00:00:00
abstract::Bacteriophage T7 RNA polymerase is stable in Escherichia coli but very susceptible to cleavage by at least one endoprotease after cell lysis. The major source of this endoprotease activity was found to be localized to the outer membrane of the cell. A rapid whole-cell assay was developed to screen different strains fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1245-1253.1988
更新日期:1988-03-01 00:00:00
abstract::Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.17.4951-4958.2000
更新日期:2000-09-01 00:00:00
abstract::Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.912-921.1990
更新日期:1990-02-01 00:00:00
abstract::We describe the isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. These mutations, designated spr(Am), were isolated and characterized in a lexA tif sfi genetic background. They abolished the sensitivity of the strain to UV light and resulted in high rates of synthesis of rec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.568-573.1979
更新日期:1979-01-01 00:00:00
abstract::Proteus swarming is the rapid cyclical population migration across surfaces by elongated cells that hyperexpress flagellar and virulence genes. The mini-Tn5 transposon mutant mns2 was isolated as a tight nonswarming mutant that did not elongate or upregulate flagellar and hemolysin genes. Individual cell motility was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.15.4741-4746.1997
更新日期:1997-08-01 00:00:00
abstract::Welker, N. E. (Western Reserve University, Cleveland, Ohio and University of Illinois, Urbana), and L. Leon Campbell. De novo synthesis of alpha-amylase by Bacillus stearothermophilus. J. Bacteriol. 86:1202-1210. 1963.-The pH optimum for the synthesis of alpha-amylase by washed-cell suspensions was 6.7. alpha-Amylase ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.6.1202-1210.1963
更新日期:1963-12-01 00:00:00
abstract::Strains of Escherichia coli K-12 mutant in the genes controlling excision repair (uvr) and genetic recombination (rec) have been studied with reference to their radiosensitivity and their ability to repair X-ray-induced single-strand breaks in deoxyribonucleic acid (DNA). Mutations in the rec genes appreciably increas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.1.49-54.1970
更新日期:1970-07-01 00:00:00
abstract::The nitrogen assimilation control protein (NAC) binds to a site within the promoter region of the histidine utilization operon (hutUH) of Klebsiella aerogenes, and NAC bound at this site activates transcription of hutUH. This NAC-binding site was characterized by a combination of random and directed DNA mutagenesis. M...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.3.578-585.1998
更新日期:1998-02-01 00:00:00
abstract::Benyesh-Melnick, Matilda (Baylor University College of Medicine, Houston, Tex.), Fern Probstmeyer, Robert McCombs, Jean P. Brunschwig, and Vladimir Vonka. Correlation between infectivity and physical virus particles in human cyto-megalovirus. J. Bacteriol. 92:1555-1561. 1966.-Infectivity titers [measured as plaque-for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.5.1555-1561.1966
更新日期:1966-11-01 00:00:00
abstract::Mycoplasma pneumoniae adherence to host cells is a multifactorial process that requires the cytadhesin P1 and additional accessory proteins. The hmw gene cluster consists of the genes p30, hmw3, and hmw1, the products of which are known to be essential for cytadherence, the rpsD gene, and six open reading frames of un...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.4978-4985.1999
更新日期:1999-08-01 00:00:00
abstract::Pantothenate kinase (CoaA) is a key regulator of coenzyme A (CoA) biosynthesis in Escherichia coli, and its activity is controlled by feedback inhibition by CoA and its thioesters. The importance of feedback inhibition in the control of the intracellular CoA levels was tested by constructing three site-directed mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.11.3410-3415.2003
更新日期:2003-06-01 00:00:00
abstract::An incompetent mutant (4S Com(-)) does not release competence-inducing factor (CF) into the culture medium and is not infected with TP-1C phage deoxyribonucleic acid (DNA) unless CF is added to the transfection assay. A TP-1C phage-resistant mutant (4S Ton-r) releases relatively large amounts of CF into the culture me...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.3.955-959.1971
更新日期:1971-06-01 00:00:00
abstract::Lundgren, D. G. (Syracuse University, Syracuse, N.Y.) and J. J. Cooney. Chemical analyses of asporogenic mutants of Bacillus cereus. J. Bacteriol. 83:1287-1293. 1962.-Bacillus cereus ATCC 4342 and three temperature-sensitive asporogenic mutants were compared regarding some basic cellular components and response to met...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.6.1287-1293.1962
更新日期:1962-06-01 00:00:00
abstract::Acclimation to high salt concentrations involves concerted changes in gene expression. For the majority of salt-regulated genes, the mechanism underlying the induction process is not known. The gene ggpS (sll1566), which encodes the glucosylglycerol-phosphate synthase responsible for the synthesis of the compatible so...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00481-10
更新日期:2010-09-01 00:00:00
abstract::It has been reported that the accumulation of K+ by Streptococcus faecalis is mediated by a transport system which required both ATP and the proton motive force (Bakker and Harold, J. Biol. Chem. 255:433-440, 1980). My results indicate that S. faecalis has a second transport system for K+. The features of this system ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.506-511.1982
更新日期:1982-05-01 00:00:00
abstract::The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.3.1096-1100.2001
更新日期:2001-02-01 00:00:00
abstract::Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.23.7530-7537.1997
更新日期:1997-12-01 00:00:00
abstract::Galactose-negative mutants of the group H Streptococcus strain Challis were obtained by treatment with nitrosoguanidine. Enzyme assays of extracts of these mutants revealed that 12 of the mutants were lacking one of the enzymes of the Leloir pathway. Thus, the Leloir pathway is the major means of galactose metabolism ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.2.512-519.1972
更新日期:1972-02-01 00:00:00
abstract::The stable inheritance of the broad-host-range plasmid RK2 is due at least in part to functions within a region located at coordinates 32.8 to 35.9 kb, termed the RK2 par locus. This locus encodes four previously identified genes in two operons (parCBA and parD; M. Gerlitz, O. Hrabak, and H. Schwab, J. Bacteriol. 172:...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.24.8119-8132.1992
更新日期:1992-12-01 00:00:00
abstract::The hyperthermophilic eubacterium Thermotoga maritima possesses an operon encoding an Hsp70 molecular chaperone protein and a protein with meaningful homology to the small heat shock protein family of chaperones. This represents the first demonstrated co-operon organization for these two important classes of molecular...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.14.4237-4244.1999
更新日期:1999-07-01 00:00:00
abstract::A homologue of the grmA spore germination gene of Bacillus megaterium and of a NaH-antiporter gene (napA) of Enterococcus hirae has been identified in Bacillus cereus 569 (ATCC 10876). The putative protein product has 58 and 43% amino acid identity with GrmA and NapA, respectively. Insertional inactivation of this B. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.2.476-482.2001
更新日期:2001-01-01 00:00:00
abstract::An increased demand for cytochromes is associated with symbiotic development and microaerobic metabolism in the bacterium Bradyrhizobium japonicum, and evidence suggests that hemB, rather than hemA, is the first essential bacterial heme synthesis gene in symbiosis with soybean. Steady-state levels of mRNA and protein ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3706-3710.1997
更新日期:1997-06-01 00:00:00
abstract::SugE of Escherichia coli, first identified as a suppressor of groEL mutations but a member of the small multidrug resistance family, has not previously been shown to confer a drug resistance phenotype. We show that high-level expression of sugE leads to resistance to a subset of toxic quaternary ammonium compounds. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.9.2543-2545.2002
更新日期:2002-05-01 00:00:00