In vivo and in vitro kinetics of nitrogenase.

abstract：:The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...

journal_title：Journal of bacteriology

authors： Peng JB,Yan WM,Bao XZ

更新日期：1994-05-01 00:00:00

abstract：:In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...

journal_title：Journal of bacteriology

authors： Wong PK,Popham D,Keener J,Kustu S

更新日期：1987-06-01 00:00:00

abstract：:Secretion vectors based on the genes from Bacillus amyloliquefaciens P for alkaline protease (aprBamP) and neutral protease (nprBamP) were constructed. With both aprBamP and nprBamP, a unique restriction site was introduced 3' of the predicted signal coding region by using the technique of oligonucleotide-directed mut...

journal_title：Journal of bacteriology

authors： Vasantha N,Thompson LD

更新日期：1986-03-01 00:00:00

abstract：:The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all prepar...

journal_title：Journal of bacteriology

authors： Volk WA

更新日期：1968-03-01 00:00:00

abstract：UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

journal_title：Journal of bacteriology

authors： Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

更新日期：2015-07-01 00:00:00

abstract：:Microtubular and lamellate membrane structures were observed at the log phase of growth in the stable L-forms of Escherichia coli cultured in the absence of penicillin. ...

journal_title：Journal of bacteriology

authors： Eda T,Kanda Y,Kimura S

更新日期：1976-09-01 00:00:00

abstract：:A lipoteichoic acid (LTA) was extracted from Listeria monocytogenes (serotype 1) by phenol-water partition and isolated by gel-filtration chromatography. The LTA exhibited amphiphilic properties by changes in gel-filtration mobility in the presence of detergent buffers and after mild base hydrolysis. In a hemagglutina...

journal_title：Journal of bacteriology

authors： Hether NW,Jackson LL

更新日期：1983-11-01 00:00:00

abstract：:One step in de novo pyridoxine (vitamin B6) and pyridoxal 5'-phosphate biosynthesis was predicted to be an oxidation catalyzed by an unidentified D-erythrose-4-phosphate dehydrogenase (E4PDH). To help identify this E4PDH, we purified the Escherichia coli K-12 gapA- and gapB-encoded dehydrogenases to homogeneity and te...

journal_title：Journal of bacteriology

authors： Zhao G,Pease AJ,Bharani N,Winkler ME

更新日期：1995-05-01 00:00:00

abstract：:Under starvation conditions, a swarm of Myxococcus xanthus cells will undergo development, a multicellular process culminating in the formation of many aggregates called fruiting bodies, each of which contains up to 100,000 spores. The mechanics of symmetry breaking and the self-organization of cells into fruiting bod...

journal_title：Journal of bacteriology

authors： Zhang H,Angus S,Tran M,Xie C,Igoshin OA,Welch RD

更新日期：2011-10-01 00:00:00

abstract：:The M-band technique was used to assess the number of attachment points of DNA to the cell membrane of Streptococcus faecalis grown at three different rates. Cells were X irradiated in liquid nitrogen and then analyzed simultaneously for the introduction of double-strand breaks into the chromosome and the degree of re...

journal_title：Journal of bacteriology

authors： Parks LC,Rigney D,Daneo-Moore L,Higgins ML

更新日期：1982-10-01 00:00:00

abstract：:The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich regio...

journal_title：Journal of bacteriology

authors： Rathsam C,Giffard PM,Jacques NA

更新日期：1993-07-01 00:00:00

abstract：:As a result of serial exposures to a mutagenic agent, N-methyl-N'-nitro-N-nitrosoguanidine, the yield of enterotoxin A produced by the last mutant in the series was increased nearly 20-fold over the amount produced by the parent Staphylococcus aureus 100. ...

journal_title：Journal of bacteriology

authors： Friedman ME,Howard MB

更新日期：1971-04-01 00:00:00

abstract：:In response to molecular oxygen and/or fixed nitrogen, the product of the Klebsiella pneumoniae nitrogen fixation L (nifL) gene inhibits NifA-mediated transcriptional activation. Nitrogen regulation of NifL function occurs at two levels: transcription of the nifLA operon is regulated by the general Ntr system, and the...

journal_title：Journal of bacteriology

authors： He L,Soupene E,Kustu S

更新日期：1997-12-01 00:00:00

abstract：:In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...

journal_title：Journal of bacteriology

authors： Myers CR,Myers JM

更新日期：1992-06-01 00:00:00

abstract：:Previous studies have indicated that a single enzyme, "kynureninase," catalyzes the reactions of l-kynurenine to anthranilate and l-3-hydroxykynurenine to 3-hydroxyanthranilate in Neurospora crassa and in other organisms. The present report describes separate enzymes which catalyze these reactions in N. crassa. The fi...

journal_title：Journal of bacteriology

authors： Gaertner FH,Cole KW,Welch GR

更新日期：1971-11-01 00:00:00

abstract：:The yfdXWUVE operon appears to encode proteins that enhance the ability of Escherichia coli MG1655 to survive under acidic conditions. Although the molecular mechanisms underlying this phenotypic behavior remain to be elucidated, findings from structural genomic studies have shown that the structure of YfdW, the prote...

journal_title：Journal of bacteriology

authors： Toyota CG,Berthold CL,Gruez A,Jónsson S,Lindqvist Y,Cambillau C,Richards NG

更新日期：2008-04-01 00:00:00

abstract：:It has been clear for over sixty years that the principal method whereby cells replicate and segregate their DNA is semiconservative. It is much less clear why it should be like this rather than, say, conservative. Recently, evidence has accumulated that supports the hypothesis that one of the functions of the cell cy...

journal_title：Journal of bacteriology

authors： Norris V

更新日期：2019-05-22 00:00:00

abstract：:A nicotinamide adenine dinucleotide-specific L-(+)-lactate dehydrogenase (LDH) (EC 1.11.27) from Actinomyces viscosus T-6-1600 was purified approximately 110-fold by a combination of diethylaminoethyl-cellulose and 0.5 M Agarose A column chromatography. The ldh was stable at 26 C, but was quite labile at temperatures ...

journal_title：Journal of bacteriology

authors： Brown AT,Christian CP,Eifert RL

更新日期：1975-06-01 00:00:00

abstract：:More than 98% of reported human listeriosis cases are caused by Listeria monocytogenes serotypes within lineages I and II. Serotypes within lineage III (4a and 4c) are commonly isolated from environmental and food specimens. We report the first complete genome sequence of a lineage III isolate, HCC23, which will be us...

journal_title：Journal of bacteriology

authors： Steele CL,Donaldson JR,Paul D,Banes MM,Arick T,Bridges SM,Lawrence ML

更新日期：2011-07-01 00:00:00

abstract：:We have compared the amino acid incorporating activities of extracts of Escherichia coli and Salmonella typhimurium in in vitro protein-synthesizing systems directed by bacterial messenger ribonucleic acid (mRNA) of both species and by the genomes of coliphages Qbeta and f2. E. coli and S. typhimurium extracts transla...

journal_title：Journal of bacteriology

authors： Bassel BA,Curry ME

更新日期：1973-11-01 00:00:00

abstract：:The penultimate step in the biosynthesis of riboflavin (vitamin B2) involves the condensation of 3,4-dihydroxy-2-butanone 4-phosphate with 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, which is catalyzed by 6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase). Pathogenic Brucella species adapted to an intr...

journal_title：Journal of bacteriology

authors： Zylberman V,Klinke S,Haase I,Bacher A,Fischer M,Goldbaum FA

更新日期：2006-09-01 00:00:00

abstract：:Two sets of genes for the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) were detected in the photosynthetic purple sulfur bacterium Chromatium vinosum by hybridization analysis with RuBisCO gene probes, cloned by using the lambda Fix vector, and designated rbcL-rbcS and rbcA-rbc...

journal_title：Journal of bacteriology

authors： Viale AM,Kobayashi H,Akazawa T

更新日期：1989-05-01 00:00:00

abstract：:Total synthesis of acetate from CO2 by Clostridium acidiurici during fermentations of hypoxanthine has been shown to involve synthesis of glycine from methylenetetrahydrofolate, CO2, and NH3. The glycine is converted to serine by the addition of methylenetetrahydrofolate, and the resulting serine is converted to pyruv...

journal_title：Journal of bacteriology

authors： Waber LJ,Wood HG

更新日期：1979-11-01 00:00:00

abstract：:Glycerol trinitrate (GTN) reductase, which enables Agrobacterium radiobacter to utilize GTN and related explosives as sources of nitrogen for growth, was purified and characterized, and its gene was cloned and sequenced. The enzyme was a 39-kDa monomeric protein which catalyzed the NADH-dependent reductive scission of...

journal_title：Journal of bacteriology

authors： Snape JR,Walkley NA,Morby AP,Nicklin S,White GF

更新日期：1997-12-01 00:00:00

abstract：:A comparison of the distribution of CO oxidation activity between soluble and particulate protein fractions obtained after disruption of CO-grown Rhodopseudomonas gelatinosa 1 by French pressure cell breakage and osmotic lysis of spheroplasts suggested that, in situ, the enzyme complex was associated with the cell mem...

journal_title：Journal of bacteriology

authors： Wakim BT,Uffen RL

更新日期：1983-01-01 00:00:00

abstract：:The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis conse...

journal_title：Journal of bacteriology

authors： Gunasekaran P,Karunakaran T,Cami B,Mukundan AG,Preziosi L,Baratti J

更新日期：1990-12-01 00:00:00

abstract：:The Escherichia coli pur regulon repressor protein was overproduced in a phage T7 expression system. The overexpressed repressor constituted approximately 35% of the soluble cellular protein. Pur repressor was purified to near homogeneity by two chromatographic steps. Hypoxanthine or guanine was required for binding o...

journal_title：Journal of bacteriology

authors： Rolfes RJ,Zalkin H

更新日期：1990-10-01 00:00:00

abstract：:The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...

journal_title：Journal of bacteriology

authors： Leroux M,Jani N,Sandler SJ

更新日期：2017-08-08 00:00:00

abstract：:The roles of three TATA binding protein (TBP) homologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using genetic and molecular approaches. Although tbp2 and tbp3 deletion mutants were readily obtained, a tbp1 mutant was not obtained, and the growth of a conditional tbp1 expre...

journal_title：Journal of bacteriology

authors： Reichlen MJ,Murakami KS,Ferry JG

更新日期：2010-03-01 00:00:00

abstract：:Using nitrogen-fixing Sinorhizobium species that interact with Medicago plants as a model system, we aimed at clarifying how sex has shaped the diversity of bacteria associated with the genus Medicago on the interspecific and intraspecific scales. To gain insights into the diversification of these symbionts, we inferr...

journal_title：Journal of bacteriology

authors： Bailly X,Olivieri I,Brunel B,Cleyet-Marel JC,Béna G

更新日期：2007-07-01 00:00:00