Membrane-DNA attachment sites in Streptococcus faecalis cells grown at different rates.

abstract：:Yeast cells growing on mineral medium plus ammonia and glucose contained high levels of nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase activity, as measured in crude extracts. After suspension of cells in fresh medium lacking glucose, there was a loss of the glutamate dehydrogenase activ...

journal_title：Journal of bacteriology

authors： Mazón MJ

更新日期：1978-02-01 00:00:00

abstract：:The gene encoding the outer membrane phosphate-selective porin protein P from Pseudomonas aeruginosa was cloned into Escherichia coli. The protein product was expressed and transported to the outer membrane of an E. coli phoE mutant and assembled into functional trimers. Expression of a product of the correct molecula...

journal_title：Journal of bacteriology

authors： Siehnel RJ,Worobec EA,Hancock RE

更新日期：1988-05-01 00:00:00

abstract：:The genes governing three of the enzymes of the isoleucine-valine biosynthetic pathway form the operon: operator-ilvA-ilvD-ilvE. The enzymes are: ilvA, l-threonine deaminase; ilvD, dihydroxy acid dehydrase; and ilvE, transaminase B. A nonsense mutation in the ilvD gene (D-ochre) and a nonsense mutation in the ilvE gen...

journal_title：Journal of bacteriology

authors： Wechsler JA,Adelberg EA

更新日期：1969-06-01 00:00:00

abstract：:The temperate phage TPW22, induced from Lactococcus lactis subsp. cremoris W22, and the evolutionarily interesting integrase of this phage were characterized. Phage TPW22 was propagated lytically on L. lactis subsp. cremoris 3107, which could also be lysogenized by site-specific integration. The attachment site (attP)...

journal_title：Journal of bacteriology

authors： Petersen A,Josephsen J,Johnsen MG

更新日期：1999-11-01 00:00:00

abstract：:The anaerobic spore former Clostridioides difficile causes significant diarrheal disease in humans and other mammals. Infection begins with the ingestion of dormant spores, which subsequently germinate within the host gastrointestinal tract. There, the vegetative cells proliferate and secrete two exotoxins, TcdA and T...

journal_title：Journal of bacteriology

authors： Edwards AN,Krall EG,McBride SM

更新日期：2020-01-02 00:00:00

abstract：:The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...

journal_title：Journal of bacteriology

authors： Inatomi K,Maeda M

更新日期：1988-12-01 00:00:00

abstract：:Chlamydiae are unusual obligately intracellular bacteria that do not synthesize detectable peptidoglycan. However, they possess genes that appear to encode products with peptidoglycan biosynthetic activity. Bioinformatic analysis predicts that chlamydial MurE possesses UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelic acid (...

journal_title：Journal of bacteriology

authors： Patin D,Bostock J,Blanot D,Mengin-Lecreulx D,Chopra I

更新日期：2009-12-01 00:00:00

abstract：:We report here direct evidence that mutations in the par locus affect protein-DNA interactions in vivo at the replication origin of plasmid pSC101. Concomitant with par-mediated plasmid stabilization, two sites in the origin region show an altered methylation pattern as detected by in vivo footprinting with dimethyl s...

journal_title：Journal of bacteriology

authors： Ingmer H,Cohen SN

更新日期：1993-09-01 00:00:00

abstract：:Microtubular structures, which were demonstrated as straight, dense-walled cylinders attached to cell membranes, were found in a stable staphylococcal L-form grown in the absence of antibiotic. ...

journal_title：Journal of bacteriology

authors： Eda T,Kanda Y,Mori C,Kimura S

更新日期：1977-12-01 00:00:00

abstract：:The homotrimeric enzyme Mt-Cam from Methanosarcina thermophila is the archetype of the gamma class of carbonic anhydrases. A search of databases queried with Mt-Cam revealed that a majority of the homologs comprise a putative subclass (CamH) in which there is major conservation of all of the residues essential for the...

journal_title：Journal of bacteriology

authors： Zimmerman SA,Tomb JF,Ferry JG

更新日期：2010-03-01 00:00:00

abstract：:Deletion of bacterial DNA fragments is stimulated in induced Mucts62 lysogens. The host genes located proximally to the prophage are more frequently lost than those which are unlinked to the Mu genome. Genes located on either side of a Mu genome are deleted in the same manner. Like the other Mu-induced rearrangements,...

journal_title：Journal of bacteriology

authors： Faelen M,Toussaint A

更新日期：1978-11-01 00:00:00

abstract：:A temperature-sensitive mutant of Escherichia coli with a temperature-dependent change in the control of initiation of DNA replication was isolated. The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC. In vitro RNA polymerase activity was temperature sensitive. The mutant grew and s...

journal_title：Journal of bacteriology

authors： Rasmussen KV,Atlung T,Kerszman G,Hansen GE,Hansen FG

更新日期：1983-04-01 00:00:00

abstract：:In Escherichia coli, T:G mismatches in specific contexts are corrected by a very short patch (VSP) repair system. Previous studies have shown that the product of gene vsr mediates correction of T:G to C:G in the 5'CTAGG/3'GGTCC context and in some related contexts. Amber mutations that arose in CAG sequences in gene c...

journal_title：Journal of bacteriology

authors： Lieb M,Rehmat S

更新日期：1995-02-01 00:00:00

abstract：:Uranyl nitrate inhibited the transport of several amino acids, a vitamin, and a disaccharide into yeast. ...

journal_title：Journal of bacteriology

authors： Maxwell WA,Metzler R,Spoerl E

更新日期：1971-03-01 00:00:00

abstract：:The Bacillus spo0H gene codes for sigma H, which, as part of the RNA polymerase holoenzyme E sigma H, is responsible for the transcription of several genes which are expressed at the beginning of the sporulation process. In this communication, we examined the regulation of the spo0H gene of Bacillus subtilis by using ...

journal_title：Journal of bacteriology

authors： Weir J,Predich M,Dubnau E,Nair G,Smith I

更新日期：1991-01-01 00:00:00

abstract：:Diacyl phthiocerol esters and their congeners are mycobacterial virulence factors. The biosynthesis of these complex lipids remains poorly understood. Insight into their biosynthesis will aid the development of rationally designed drugs that inhibit their production. In this study, we investigate a biosynthetic step r...

journal_title：Journal of bacteriology

authors： Onwueme KC,Vos CJ,Zurita J,Soll CE,Quadri LE

更新日期：2005-07-01 00:00:00

abstract：:A 16-kb BamHI fragment of the lactose plasmid pNZ63 from Leuconostoc lactis NZ6009 was cloned in Escherichia coli MC1061 by using pACYC184 and was found to express a functional beta-galactosidase. Deletion and complementation analysis showed that the coding region for beta-galactosidase was located on a 5.8-kb SalI-Ba...

journal_title：Journal of bacteriology

authors： David S,Stevens H,van Riel M,Simons G,de Vos WM

更新日期：1992-07-01 00:00:00

abstract：:Strains of avian pathogenic Escherichia coli (APEC), the common pathogen of avian colibacillosis, encounter reactive oxygen species (ROS) during the infection process. Superoxide dismutases (SODs), acting as antioxidant factors, can protect against ROS-mediated host defenses. Our previous reports showed that the sodA ...

journal_title：Journal of bacteriology

authors： Gao Q,Xia L,Wang X,Ye Z,Liu J,Gao S

更新日期：2019-02-25 00:00:00

abstract：:Polyribosomes detected in extracts of spores harvested with water also were found in extracts prepared from spores harvested with nonaqueous fluids. ...

journal_title：Journal of bacteriology

authors： Brambl R

更新日期：1975-06-01 00:00:00

abstract：:Mutants for 9 of the 10 steps in histidine biosynthesis have been isolated and identified by enzyme assay. Each locus has been mapped in relation to the aro cluster and to other histidine loci by deoxyribonucleic acid-mediated transformation. The genes which code for enzymes 3, 6, and 8 of the pathway are linked to th...

journal_title：Journal of bacteriology

authors： Chapman LF,Nester EW

更新日期：1969-03-01 00:00:00

abstract：:Microarray-based analysis of the transcriptional profiles of the genetically distinct Staphylococcus aureus strains COL, GP268, and Newman indicate that a total of 251 open reading frames (ORFs) are influenced by sigmaB activity. While sigmaB was found to positively control 198 genes by a factor of > or =2 in at least...

journal_title：Journal of bacteriology

authors： Bischoff M,Dunman P,Kormanec J,Macapagal D,Murphy E,Mounts W,Berger-Bächi B,Projan S

更新日期：2004-07-01 00:00:00

abstract：:Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

journal_title：Journal of bacteriology

authors： Rocco JM,Churchward G

更新日期：2006-03-01 00:00:00

abstract：:A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. A systematic searc...

journal_title：Journal of bacteriology

authors： Wirth R,An FY,Clewell DB

更新日期：1986-03-01 00:00:00

abstract：:Several authors have suggested that the SOS-associated (sfiA-dependent) system of division inhibition, normally induced by perturbations of DNA replication, also regulates steady-state (unperturbed) cell division. The present work shows that mean cell mass is identical in sfiA+ and sfiA mutant cultures during steady-s...

journal_title：Journal of bacteriology

authors： Huisman O,Jacques M,D'ari R,Caro L

更新日期：1983-02-01 00:00:00

abstract：:Multicopy single-stranded DNA is found as a small single-stranded RNA-DNA complex in certain wild-type strains of Escherichia coli as well as in other gram-negative bacteria. Using the promoter region of the previously characterized retron-Ec107 from E. coli ECOR70, I constructed a chromosomally located lacZ operon fu...

journal_title：Journal of bacteriology

authors： Herzer PJ

更新日期：1996-08-01 00:00:00

abstract：:In the chloroplasts of higher plants and algae, the biosynthesis of the chlorophyll precursor delta-aminolevulinic acid (ALA) involves at least three enzymes and a tRNA species. Here we demonstrate that in cell extracts of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 ALA was formed from glutamate i...

journal_title：Journal of bacteriology

authors： O'Neill GP,Peterson DM,Schön A,Chen MW,Söll D

更新日期：1988-09-01 00:00:00

abstract：:The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (molecular weight, 9,00...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schwartz M

更新日期：1983-10-01 00:00:00

abstract：:It was shown in an accompanying paper (Buck and Groman, J. Bacteriol. 148: 131-142, 1981) that gamma-tsr-1 phage stocks produced by heat induction of lysogens are a mixture of two phages which differ in the content of their deoxyribonucleic acid (DNA). This difference is evidenced by the appearance of "heterogeneous" ...

journal_title：Journal of bacteriology

authors： Buck GA,Groman NB

更新日期：1981-10-01 00:00:00

abstract：:A homologue of the grmA spore germination gene of Bacillus megaterium and of a NaH-antiporter gene (napA) of Enterococcus hirae has been identified in Bacillus cereus 569 (ATCC 10876). The putative protein product has 58 and 43% amino acid identity with GrmA and NapA, respectively. Insertional inactivation of this B. ...

journal_title：Journal of bacteriology

authors： Thackray PD,Behravan J,Southworth TW,Moir A

更新日期：2001-01-01 00:00:00

abstract：:Strain IMCC3088, cultivated from the Yellow Sea, is a novel isolate belonging to the OM60/NOR5 clade and is closely related to clone OM241, Congregibacter litoralis, and strain HTCC2080. Here, the genome sequence of strain IMCC3088 is presented, showing the absence of photosynthetic gene clusters and the presence of p...

journal_title：Journal of bacteriology

authors： Jang Y,Oh HM,Kang I,Lee K,Yang SJ,Cho JC

更新日期：2011-07-01 00:00:00