Abstract:
:It was shown in an accompanying paper (Buck and Groman, J. Bacteriol. 148: 131-142, 1981) that gamma-tsr-1 phage stocks produced by heat induction of lysogens are a mixture of two phages which differ in the content of their deoxyribonucleic acid (DNA). This difference is evidenced by the appearance of "heterogeneous" (HET) fragments in restriction enzyme digests of gamma-tsr-1 phage DNA. It was estimated that 20 to 80% of the phage in these lysates produced HET fragments. The appearance of HET fragments correlated with the appearance of a DNA insertion (DI-1) in the gamma phage genome as revealed in heteroduplexes of DNA from gamma-tsr-1 and beta corynebacteriophages. The HET fragments were seen in DNA from heat-induced lysates, but not in DNA from phage stocks produced by lytic infection. By DNA-DNA hybridization analysis it was shown that a fraction of gamma-tsr-1 phages from heat-induced lysates carried an insertion of bacterial DNA in the vegetative phage attachment site (attP), and that this insertion was responsible for the formation of HET fragments. Since the phage produced by this event carried a complete phage genome plus a small segment of bacterial DNA, they were called transducing elements. On the basis of these facts it was concluded that heat-induced gamma-tsr-1 prophage was excised at an abnormal site at a very high frequency. Abnormal excision was highly specific, and the change in excision specificity occurred simultaneously with the spontaneous mutation of the phage to heat inducibility. From this and other data it was postulated that a mutation in the immune repressor was reponsible for an alteration in the specificity of the normal excision process. This distinguishes the mechanism of formation of gamma-tsr-1 transducing elements from that employed by other phages. A second DNA insertion (DI-2) in the tox (diphtheria toxin) gene of gamma-tsr-1 and gamma-tsr-2 was also identified as an insertion of bacterial DNA. The DI-2 insertion had a stem-and-loop structure similar to that seen in heteroduplexes visualizing transposons or insertion elements. It seems likely that gamma wild-type phage, which is mutant for tox, was originally tox(+), but that transposition of bacterial DNA into the gene inactivated it.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Buck GA,Groman NBdoi
10.1128/JB.148.1.143-152.1981subject
Has Abstractpub_date
1981-10-01 00:00:00pages
143-52issue
1eissn
0021-9193issn
1098-5530journal_volume
148pub_type
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doi:10.1128/JB.164.2.578-584.1985
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doi:10.1128/JB.120.1.495-501.1974
更新日期:1974-10-01 00:00:00
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doi:10.1128/JB.150.3.1485-1488.1982
更新日期:1982-06-01 00:00:00
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更新日期:1970-11-01 00:00:00
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更新日期:1986-01-01 00:00:00
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更新日期:2003-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1995-11-01 00:00:00
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更新日期:1988-04-01 00:00:00