Abstract:
:A temperature-sensitive mutant of Escherichia coli with a temperature-dependent change in the control of initiation of DNA replication was isolated. The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC. In vitro RNA polymerase activity was temperature sensitive. The mutant grew and synthesized DNA at 30 degrees C as did the wild type. After a shift to 39 degrees C, a temperature still permissive for growth, the mutant increased its origin concentration more than twofold. After a shift from 39 to 30 degrees C, initiation of DNA replication was inhibited until the normal origin concentration was reestablished.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Rasmussen KV,Atlung T,Kerszman G,Hansen GE,Hansen FGdoi
10.1128/JB.154.1.443-451.1983subject
Has Abstractpub_date
1983-04-01 00:00:00pages
443-51issue
1eissn
0021-9193issn
1098-5530journal_volume
154pub_type
杂志文章abstract::Virulent bacteria of the genus Yersinia secrete a number of virulence determinants called Yops. These proteins lack typical signal sequences and are not posttranslationally processed. Two gene loci have been identified as being involved in the specific Yop secretion system (G. Cornelis, p. 231-265, In C. E. Hormache, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.9.2619-2626.1994
更新日期:1994-05-01 00:00:00
abstract::Organic compounds exhibit various levels of toxicity toward living organisms based upon their ability to insert into biological membranes and disrupt normal membrane function. The primary mechanism responsible for organic solvent tolerance in many bacteria is energy-dependent extrusion via efflux pumps. One such bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00149-11
更新日期:2011-06-01 00:00:00
abstract::The induction of paraffin oxidation in intact cells of Pseudomonas aeruginosa was investigated. Oxidation of (14)C-heptane by cell-free extracts of adapted cells showed that the activity of whole cells is a reliable reflection of the synthesis of the first enzyme in the degradation of n-alkanes. Induction was signific...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.3.706-712.1968
更新日期:1968-09-01 00:00:00
abstract::By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::The model of pneumonia produced by intranasal inoculation of Mycoplasma pulmonis in gnotobiotic mice provided the opportunity to study the localization and identification of the infecting organisms in the tissues by immunofluorescence techniques. Frozen sections of pneumonic mouse lung were fixed in acetone, layered w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.1.250-258.1968
更新日期:1968-07-01 00:00:00
abstract::Here, we present the complete genome sequence of Rhodococcus pyridinivorans AK37 strain NCAIM PB1376, which was isolated from an oil-polluted site in Hungary. R. pyridinivorans AK37 is an aerobic, nonsporulating, nonmotile, gram-positive bacterium with remarkable aromatic-decomposing activity. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06603-11
更新日期:2012-03-01 00:00:00
abstract::The mutualistic symbiont Vibrio fischeri builds a symbiotic biofilm during colonization of squid hosts. Regulation of the exopolysaccharide component, termed Syp, has been examined in strain ES114, where production is controlled by a phosphorelay that includes the inner membrane hybrid histidine kinase RscS. Most stra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00033-19
更新日期:2019-04-09 00:00:00
abstract::Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00892-07
更新日期:2007-10-01 00:00:00
abstract::Izaki, Kazuo (University of Tokyo, Tokyo, Japan), Kan Kiuchi, and Kei Arima. Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bacteriol. 91:628-633. 1966.-A decrease in the uptake of tetracycline occurred concurrently with a rise in the level of resistanc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.628-633.1966
更新日期:1966-02-01 00:00:00
abstract::Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize heme from ALA. Sterol-depleted cells not exposed to ALA or strain PFY3 cells, with a double heme mutation, exposed to ALA did n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3707-3711.1987
更新日期:1987-08-01 00:00:00
abstract::The sugar composition of lipopolysaccharide (LPS) isolated from whole cells of Alteromonas haloplanktis 214 (previously referred to as marine pseudomonas B-16, ATCC 19855), variant 3, of the lipid A, core, and side-chain fractions derived from it, and of the LPS fractions (LPS I, II, and III) obtained by subjecting it...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.158-167.1978
更新日期:1978-10-01 00:00:00
abstract::Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.5.1554-1567.1992
更新日期:1992-03-01 00:00:00
abstract::The colH gene encoding a collagenase was cloned from Clostridium histolyticum JCM 1403. Nucleotide sequencing showed a major open reading frame encoding a 116-kDa protein of 1,021 amino acid residues. The deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, HEX...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.21.6489-6496.1994
更新日期:1994-11-01 00:00:00
abstract::Vacuoles project streams of vesicles and membranous tubules into the yeast bud where they fuse, founding the daughter cell organelle, vac5-1, which encodes a truncated form of the Pho80 cyclin, inhibits normal vacuole inheritance. An in vitro inheritance assay which measures the fusion of vacuoles serves as a model fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.14.4047-4051.1996
更新日期:1996-07-01 00:00:00
abstract::Because very little is known about cell division in noncylindrical bacteria and cyanobacteria, we investigated 10 putative cytokinetic proteins in the unicellular spherical cyanobacterium Synechocystis strain PCC 6803. Concerning the eight penicillin-binding proteins (PBPs), which define three classes, we found that S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00620-09
更新日期:2009-08-01 00:00:00
abstract::Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.1.300-308.1971
更新日期:1971-10-01 00:00:00
abstract::Daniel, John W. (University of Wisconsin, Madison), Karlee L. Babcock, Alice H. Sievert, and Harold P. Rusch. Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum. J. Bacteriol. 86:324-331. 1963.-An isolate of Physarum polycephalum was maintained in submersed pure culture in a co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.2.324-331.1963
更新日期:1963-08-01 00:00:00
abstract::Type III secretion systems (T3SSs) are essential virulence devices for many gram-negative bacteria that are pathogenic for plants, animals, and humans. They serve to translocate virulence effector proteins directly into eukaryotic host cells. T3SSs are composed of a large cytoplasmic bulb and a transmembrane region in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01004-08
更新日期:2009-01-01 00:00:00
abstract::The major acid-soluble spore proteins (ASSPs) of Bacillus subtilis were detected by immunoprecipitation of radioactively labeled in vitro- and in vivo-synthesized proteins. ASSP synthesis in vivo began 2 h after the initiation of sporulation (t2) and reached its maximum rate at t7. This corresponded to the time of syn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.7.2247-2258.1997
更新日期:1997-04-01 00:00:00
abstract::Mutagenesis of Nitrosomonas europaea was achieved by electroporation and recombination. To demonstrate this, an aminoglycoside 3'-phosphotransferase (kan) gene was specifically inserted into each of the three gene copies of hao individually. Southern hybridizations and PCR analysis showed the incorporation of the kan ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3710-3714.1996
更新日期:1996-07-01 00:00:00
abstract::Evident in its name, the gastric pathogen Helicobacter pylori has a helical cell morphology which facilitates efficient colonization of the human stomach. An improved light-focusing strategy allowed us to robustly distinguish even subtle perturbations of H. pylori cell morphology by deviations in light-scattering prop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00724-18
更新日期:2019-06-21 00:00:00
abstract::Porphyromonas gingivalis, an obligate anaerobe, exhibits a relatively high degree of aerotolerance and possesses superoxide dismutase (SOD) which is induced by exposure to air. To clarify roles for SOD in this organism, the gene encoding SOD (sod) on the P. gingivalis chromosome was disrupted in a gene-directed way by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.1939-1943.1994
更新日期:1994-04-01 00:00:00
abstract::Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate. UV sensitivity ranges from moderate to extreme, and four of the mutants are also sensitive to nitrous acid. Ranking in terms of UV sensitivity does not parallel ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.3.571-575.1967
更新日期:1967-09-01 00:00:00
abstract::YoeB is a bacterial toxin encoded by the yefM-yoeB toxin-antitoxin system found in various bacterial genomes. Here, we show that Staphylococcus aureus contains two YoeB homologues, both of which function as ribosome-dependent mRNA interferases to inhibit translation initiation in a manner identical to that of YoeB-ec ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00623-09
更新日期:2009-09-01 00:00:00
abstract::Escherichia coli has a formate hydrogenlyase system which allows it to maintain an electron balance during anaerobic growth by passing electrons from formate to H+ ions, thus generating H2. The Mu d1(Ap lac) bacteriophage was used to generate mutants that were defective in passing electrons from formate to benzyl viol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.180-186.1984
更新日期:1984-04-01 00:00:00
abstract::While coagulase-negative staphylococci (CoNS), with their ability to form a thick, multilayered biofilm on foreign bodies, have been identified as the major cause of implant-associated infections, no data are available about biofilm formation by staphylococcal small-colony variants (SCVs). In the past years, a number ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00160-07
更新日期:2007-06-01 00:00:00
abstract::A new alginate regulatory gene, algQ, was identified in a chromosomal region which, when tandemly amplified, induces mucoidy in Pseudomonas aeruginosa. The algQ gene was found closely linked to the previously identified algR gene. Both algQ and algR were required for transcription of the key alginate biosynthetic gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3680-3688.1989
更新日期:1989-07-01 00:00:00
abstract::We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3513-3522.1988
更新日期:1988-08-01 00:00:00
abstract::We have isolated and determined the nucleotide sequence of a 1,626-base-pair fragment from R-plasmid R483 which encodes a trimethoprim-resistant dihydrofolate reductase. Analysis of the nucleotide sequence of this fragment revealed the presence of two open reading frames, each sufficient to encode polypeptides of appr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1001-1008.1983
更新日期:1983-09-01 00:00:00