Conditional change of DNA replication control in an RNA polymerase mutant of Escherichia coli.

Abstract:

:A temperature-sensitive mutant of Escherichia coli with a temperature-dependent change in the control of initiation of DNA replication was isolated. The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC. In vitro RNA polymerase activity was temperature sensitive. The mutant grew and synthesized DNA at 30 degrees C as did the wild type. After a shift to 39 degrees C, a temperature still permissive for growth, the mutant increased its origin concentration more than twofold. After a shift from 39 to 30 degrees C, initiation of DNA replication was inhibited until the normal origin concentration was reestablished.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rasmussen KV,Atlung T,Kerszman G,Hansen GE,Hansen FG

doi

10.1128/JB.154.1.443-451.1983

subject

Has Abstract

pub_date

1983-04-01 00:00:00

pages

443-51

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

154

pub_type

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