Regulation of ergosterol biosynthesis and sterol uptake in a sterol-auxotrophic yeast.

Abstract:

:Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize heme from ALA. Sterol-depleted cells not exposed to ALA or strain PFY3 cells, with a double heme mutation, exposed to ALA did not exhibit inhibition of sterol uptake. Addition of ALA to sterol-depleted FY3 stimulated production of a high endogenous concentration of 2,3-oxidosqualene (25.55 micrograms mg-1 [dry weight]) at 24 h, whereas FY3 not exposed to ALA or PFY3 exposed to ALA did not accumulate 2,3-oxidosqualene. The high concentration of 2,3-oxidosqualene in FY3 with ALA decreased, and 2,3;22,23-dioxidosqualene increased to a very high level. The elevation of 2,3-oxidosqualene by ALA was correlated with a fivefold increase in the activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.34). The enhanced activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase was prevented by cycloheximide but not chloramphenicol and was dependent on a fermentative energy source. Inhibition of sterol uptake could not be attributed to 2,3-oxidosqualene or 2,3;22,23-dioxidosqualene but was due to a nonsaturating level of ergosterol produced as a consequence of heme competency through a leaky erg7 mutation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Lorenz RT,Parks LW

doi

10.1128/jb.169.8.3707-3711.1987

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

3707-11

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

杂志文章
  • Reconstitution of an active lactose carrier in vivo by simultaneous synthesis of two complementary protein fragments.

    abstract::Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5374-5381.1990

    authors: Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

    更新日期:1990-09-01 00:00:00

  • Architecture of the cell envelope of Chlamydia psittaci 6BC.

    abstract::The cysteine-rich envelope proteins of the elementary body form of chlamydiae are thought to be located in the outer membrane on the basis of their insolubility in the weak anionic detergent N-lauryl sarcosinate (Sarkosyl). We found, however, that the insolubility of the small (EnvA) and the large (EnvB) cysteine-rich...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.4.877-882.1995

    authors: Everett KD,Hatch TP

    更新日期:1995-02-01 00:00:00

  • Transcriptional phase variation of a type III restriction-modification system in Helicobacter pylori.

    abstract::Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.23.6615-6624.2002

    authors: de Vries N,Duinsbergen D,Kuipers EJ,Pot RG,Wiesenekker P,Penn CW,van Vliet AH,Vandenbroucke-Grauls CM,Kusters JG

    更新日期:2002-12-01 00:00:00

  • Characterization of xylanolytic enzymes in Clostridium cellulovorans: expression of xylanase activity dependent on growth substrates.

    abstract::Xylanase activity of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized. Most of the activity was secreted into the growth medium when the bacterium was grown on xylan. Furthermore, when the extracellular material was separated into cellulosomal and noncellulosomal fractions...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.24.7037-7043.2001

    authors: Kosugi A,Murashima K,Doi RH

    更新日期:2001-12-01 00:00:00

  • Pilus production in Acinetobacter baumannii is growth phase dependent and essential for natural transformation.

    abstract::Acinetobacter baumannii is a severe threat to human health as a frequently multidrug-resistant hospital-acquired pathogen. Part of the danger from this bacterium comes from its genome plasticity and ability to evolve quickly by taking up and recombining external DNA into its own genome in a process called natural comp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00034-21

    authors: Vesel N,Blokesch M

    更新日期:2021-01-25 00:00:00

  • Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid.

    abstract::An IMP dehydrogenase gene was isolated from Candida albicans on a approximately 2.9-kb XbaI genomic DNA fragment. The putative Candida IMP dehydrogenase gene (IMH3) encodes a protein of 521 amino acids with extensive sequence similarity to the IMP dehydrogenases of Saccharomyces cerevisiae and various other organisms....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.7.2331-2338.1997

    authors: Köhler GA,White TC,Agabian N

    更新日期:1997-04-01 00:00:00

  • Purification and partial characterization of the principal deoxyribonucleic acid polymerase from Mycoplasmatales.

    abstract::In this report we present the first description of the isolation and partial characterization of the deoxyribonucleic acid (DNA) polymerase activity from two species of Mycoplasmatales, Mycoplasma orale type 1 and M. hyorhinis. We have identified only a single DNA polymerase species in the mycoplasma crude extracts, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.641-649.1977

    authors: Mills LB,Stanbridge EJ,Sedwick WD,Korn D

    更新日期:1977-11-01 00:00:00

  • Apparent and real recombination frequencies in multicopy plasmids: the need for a novel approach in frequency determination.

    abstract::Recombination studies of bacteria are often carried out with multicopy plasmids, and recombination frequencies are often deduced from the proportion of cells in the population that express a recombinant phenotype. These frequencies should however be called apparent frequencies, since detection of the recombinant cells...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.3.754-761.1997

    authors: Chédin F,Dervyn R,Ehrlich SD,Noirot P

    更新日期:1997-02-01 00:00:00

  • Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: purification and characterization of the enzyme.

    abstract::The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a pote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.5.1409-1414.1999

    authors: Peciña A,Pascual A,Paneque A

    更新日期:1999-03-01 00:00:00

  • Isolation and characterization of cell wall-defective variants of Bacillus subtilis and Bacillus licheniformis.

    abstract::Quantitative mass conversion of intact bacterial cells of Bacillus subtilis and B. licheniformis to L-phase variants has been effected after lysozyme treatment. After subculture of the unstable L-phase variants for several months in the presence of methicillin, stable L-phase variants were obtained which grew in the a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.1.456-465.1973

    authors: Wyrick PB,Rogers HJ

    更新日期:1973-10-01 00:00:00

  • Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa.

    abstract::The slime polysaccharides produced by Pseudomonas aeruginosa isolated from a variety of human infections were investigated. Slime production in culture seemed optimal when adequate amounts of carbohydrate were present and under conditions of either high osmotic pressure or inadequate protein supply. The polysaccharide...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.915-924.1973

    authors: Evans LR,Linker A

    更新日期:1973-11-01 00:00:00

  • Effect of temperature on Pseudomonas fluorescens chemotaxis.

    abstract::The effects of temperature and attractants on chemotaxis in psychrotrophic Pseudomonas fluorescens were examined using the Adler capillary assay technique. Several organic acids, amino acids, and uronic acids were shown to be attractants, whereas glucose and its oxidation products, gluconate and 2-ketogluconate, elici...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.338-342.1980

    authors: Lynch WH

    更新日期:1980-07-01 00:00:00

  • Cloning, nucleotide sequence, and hybridization studies of the type IIb heat-labile enterotoxin gene of Escherichia coli.

    abstract::Type IIb heat-labile enterotoxin (LT-IIb) is produced by Escherichia coli 41. Restriction fragments of total cell DNA from strain 41 were cloned into a cosmid vector, and one cosmid clone that encoded LT-IIb was identified. The genes for LT-IIb were subcloned into a variety of plasmids, expressed in minicells, sequenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4945-4952.1989

    authors: Pickett CL,Twiddy EM,Coker C,Holmes RK

    更新日期:1989-09-01 00:00:00

  • Genetic and sequence analyses of a Pseudomonas denitrificans DNA fragment containing two cob genes.

    abstract::A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complement...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.6058-6065.1991

    authors: Cameron B,Guilhot C,Blanche F,Cauchois L,Rouyez MC,Rigault S,Levy-Schil S,Crouzet J

    更新日期:1991-10-01 00:00:00

  • Reduce, Induce, Thrive: Bacterial Redox Sensing during Pathogenesis.

    abstract::The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00128-18

    authors: Reniere ML

    更新日期:2018-08-10 00:00:00

  • Regulation of enzyme synthesis in the tryptophan pathway of Acinetobacter calcoaceticus.

    abstract::In Acinetobacter calcoaceticus the seven genes coding for the enzymes responsible for tryptophan synthesis map at three chromosomal locations. Two three-gene clusters, one (trpGDC) specifying the small subunit of anthranilate synthase, phosphoribosyl transferase, and indoleglycerol phosphate synthase and the other (tr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.1.367-379.1976

    authors: Cohn W,Crawford IP

    更新日期:1976-07-01 00:00:00

  • Characterization of exochelins of Mycobacterium avium: evidence for saturated and unsaturated and for acid and ester forms.

    abstract::Mycobacterium avium secretes iron-binding siderophores called exochelins. The exochelins from M. avium have previously been reported to have unsaturated side chains that terminate in carboxylic acid. In contrast, our data show the side chains to be both saturated and unsaturated and to terminate with either a carboxyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.21.6394-6398.1996

    authors: Wong DK,Gobin J,Horwitz MA,Gibson BW

    更新日期:1996-11-01 00:00:00

  • Horizontal gene transfer and homologous recombination drive the evolution of the nitrogen-fixing symbionts of Medicago species.

    abstract::Using nitrogen-fixing Sinorhizobium species that interact with Medicago plants as a model system, we aimed at clarifying how sex has shaped the diversity of bacteria associated with the genus Medicago on the interspecific and intraspecific scales. To gain insights into the diversification of these symbionts, we inferr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00105-07

    authors: Bailly X,Olivieri I,Brunel B,Cleyet-Marel JC,Béna G

    更新日期:2007-07-01 00:00:00

  • Identification of an Escherichia coli operon required for formation of the O-antigen capsule.

    abstract::Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5259-5266.2005

    authors: Peleg A,Shifrin Y,Ilan O,Nadler-Yona C,Nov S,Koby S,Baruch K,Altuvia S,Elgrably-Weiss M,Abe CM,Knutton S,Saper MA,Rosenshine I

    更新日期:2005-08-01 00:00:00

  • A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.

    abstract::In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3864-3871.1991

    authors: Vartak NB,Liu L,Wang BM,Berg CM

    更新日期:1991-06-01 00:00:00

  • Identification of FkpA as a key quality control factor for the biogenesis of outer membrane proteins under heat shock conditions.

    abstract::The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp do...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01069-13

    authors: Ge X,Lyu ZX,Liu Y,Wang R,Zhao XS,Fu X,Chang Z

    更新日期:2014-02-01 00:00:00

  • Influence of temperature on tRNA modification in archaea: Methanococcoides burtonii (optimum growth temperature [Topt], 23 degrees C) and Stetteria hydrogenophila (Topt, 95 degrees C).

    abstract::We report the first study of tRNA modification in psychrotolerant archaea, specifically in the archaeon Methanococcoides burtonii grown at 4 and 23 degrees C. For comparison, unfractionated tRNA from the archaeal hyperthermophile Stetteria hydrogenophila cultured at 93 degrees C was examined. Analysis of modified nucl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.18.5483-5490.2003

    authors: Noon KR,Guymon R,Crain PF,McCloskey JA,Thomm M,Lim J,Cavicchioli R

    更新日期:2003-09-01 00:00:00

  • Chloroflexus aurantiacus has 30S ribosomal subunits of the eubacterial type.

    abstract::Ribosomal subunits from Chloroflexus aurantiacus were isolated and examined by sucrose gradient sedimentation, gel electrophoresis, and electron microscopy. The 30S subunits had all the characteristic structural features of other eubacterial 30S subunits. The data support the proposal that the absence of the archaebac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.2.900-902.1983

    authors: Henderson E,Pierson BK,Lake JA

    更新日期:1983-08-01 00:00:00

  • Beta-2-linked glucans secreted by fast-growing species of Rhizobium.

    abstract::Fast-growing species of Rhizobium were found to secrete low-molecular-weight beta-2-linked glucans when cultured in synthetic liquid medium. These glucans are quite similar to beta-2-linked glucans produced by species of Agrobacterium. No reducing terminus was detected in these glucans. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.1.243-248.1980

    authors: York WS,McNeil M,Darvill AG,Albersheim P

    更新日期:1980-04-01 00:00:00

  • Mapping of rfa Genes in Salmonella typhimurium by ES18 and P22 Transduction and by Conjugation.

    abstract::Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.48-57.1972

    authors: Kuo TT,Stocker BA

    更新日期:1972-10-01 00:00:00

  • Tungsten and molybdenum regulation of formate dehydrogenase expression in Desulfovibrio vulgaris Hildenborough.

    abstract::Formate is an important energy substrate for sulfate-reducing bacteria in natural environments, and both molybdenum- and tungsten-containing formate dehydrogenases have been reported in these organisms. In this work, we studied the effect of both metals on the levels of the three formate dehydrogenases encoded in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00042-11

    authors: da Silva SM,Pimentel C,Valente FM,Rodrigues-Pousada C,Pereira IA

    更新日期:2011-06-01 00:00:00

  • Mutagenic DNA repair in enterobacteria.

    abstract::Sixteen species of enterobacteria have been screened for mutagenic DNA repair activity. In Escherichia coli, mutagenic DNA repair is encoded by the umuDC operon. Synthesis of UmuD and UmuC proteins is induced as part of the SOS response to DNA damage, and after induction, the UmuD protein undergoes an autocatalytic cl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5604-5611.1991

    authors: Sedgwick SG,Ho C,Woodgate R

    更新日期:1991-09-01 00:00:00

  • A Ti plasmid-encoded enzyme required for degradation of mannopine is functionally homologous to the T-region-encoded enzyme required for synthesis of this opine in crown gall tumors.

    abstract::The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3285-3292.1996

    authors: Kim KS,Chilton WS,Farrand SK

    更新日期:1996-06-01 00:00:00

  • SIGNIFICANCE OF SALMONELLAE ISOLATED FROM APPARENTLY HEALTHY MICE.

    abstract::Morello, Josephine A. (Boston University School of Medicine, Boston, Mass.), Teresa A. DiGenio, and Edgar E. Baker. Significance of salmonellae isolated from apparently healthy mice. J. Bacteriol. 89:1460-1464. 1965.-Three species of Salmonella were isolated from three groups of apparently healthy laboratory mice. The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.6.1460-1464.1965

    authors: MORELLO JA,DIGENIO TA,BAKER EE

    更新日期:1965-06-01 00:00:00

  • Characteristics and osmoregulatory roles of uptake systems for proline and glycine betaine in Lactococcus lactis.

    abstract::Lactococcus lactis subsp. lactis ML3 contains high pools of proline or betaine when grown under conditions of high osmotic strength. These pools are created by specific transport systems. A high-affinity uptake system for glycine betaine (betaine) with a Km of 1.5 microM is expressed constitutively. The activity of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5438-5444.1993

    authors: Molenaar D,Hagting A,Alkema H,Driessen AJ,Konings WN

    更新日期:1993-09-01 00:00:00