The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression.

Abstract:

:We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no more than 4 base pairs downstream from the start site of transcription, on which basis we infer that spoIVC is regulated at the level of transcription initiation. Using a transcriptional fusion of the spoIVC gene to the lacZ gene of Escherichia coli, we found that spoIVC expression was turned on at the third to fourth hour of sporulation (at about the developmental stage [IV] that its products are required in spore formation) and that this transcription was largely restricted to the mother cell chamber of the sporangium. Mutations in many different spo genes (causing blocks at stages 0 to V) were found to influence (negatively and positively) the level of spoIVC expression. Our results distinguish the mode of spoIVC regulation from that of previously studied sporulation genes and indicate that it is representative of a new regulon of mother cell-specific gene expression.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kunkel B,Sandman K,Panzer S,Youngman P,Losick R

doi

10.1128/jb.170.8.3513-3522.1988

subject

Has Abstract

pub_date

1988-08-01 00:00:00

pages

3513-22

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

170

pub_type

杂志文章
  • Flexible Hinges in Bacterial Chemoreceptors.

    abstract::Transmembrane bacterial chemoreceptors are extended, rod-shaped homodimers with ligand-binding sites at one end and interaction sites for signaling complex formation and histidine kinase control at the other. There are atomic-resolution structures of chemoreceptor fragments but not of intact, membrane-inserted recepto...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00593-17

    authors: Akkaladevi N,Bunyak F,Stalla D,White TA,Hazelbauer GL

    更新日期:2018-02-07 00:00:00

  • CHARACTERISTICS OF CLOSTRIDIUM PERFRINGENS STRAINS ASSOCIATED WITH FOOD AND FOOD-BORNE DISEASE.

    abstract::Hall, Herbert E. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Robert Angelotti, Keith H. Lewis, and Milton J. Foter. Characteristics of Clostridium perfringens strains associated with food and food-borne disease. J. Bacteriol. 85:1094-1103. 1963.-A total of 83 strains of Clostridium perfringens-30 f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.5.1094-1103.1963

    authors: HALL HE,ANGELOTTI R,LEWIS KH,FOTER MJ

    更新日期:1963-05-01 00:00:00

  • Chromosome replication and cell division in plasmid-containing Escherichia coli B/r.

    abstract::The kinetics of chromosome replication and cell division have been examined in recA mutants of Escherichia coli B/r containing F' plasmids of various sizes. Plasmid-mediated alterations in growth properties were detected only with the presence of the larger F' plasmids, and were reflected in decreased mean cell sizes ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.3.1151-1157.1979

    authors: Weinberger M,Helmstetter CE

    更新日期:1979-03-01 00:00:00

  • Restoration by ubiquinone of Azotobacter vinelandii reduced nicotinamide adenine dinucleotide oxidase activity.

    abstract::Extraction with pentane virtually abolished reduced nicotinamide adenine dinucleotide oxidase activity in small particles from Azotobacter vinelandii, but activity was largely restored by added ubiquinone. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.311-313.1969

    authors: Swank RT,Burris RH

    更新日期:1969-04-01 00:00:00

  • The dual-specificity protein phosphatase Yvh1p acts upstream of the protein kinase mck1p in promoting spore development in Saccharomyces cerevisiae.

    abstract::Diploid Saccharomyces cerevisiae cells induce YVH1 expression and enter the developmental pathway, leading to sporulation when starved for nitrogen. We show that yvh1 disruption causes a defect in spore maturation; overexpression of MCK1 or IME1 suppresses this yvh1 phenotype. While mck1 mutations are epistatic to tho...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.17.5219-5224.1999

    authors: Beeser AE,Cooper TG

    更新日期:1999-09-01 00:00:00

  • Two genes for carbohydrate catabolism are divergently transcribed from a region of DNA containing the hexC locus in Pseudomonas aeruginosa PAO1.

    abstract::The hexC locus of Pseudomonas aeruginosa PAO1 was localized to a 247-bp segment of chromosomal DNA on the multicopy broad-host-range vector pRO1614. The presence of this plasmid (pPZ196) in strain PAO1 produced the so-called "hexC effect," a two- to ninefold increase in the activities of four carbohydrate catabolism e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.15.4700-4709.1994

    authors: Temple L,Sage A,Christie GE,Phibbs PV Jr

    更新日期:1994-08-01 00:00:00

  • The periplasmic protein MppA requires an additional mutated locus to repress marA expression in Escherichia coli.

    abstract::Escherichia coli strain TP985, which has an insertional mutation in the gene for the periplasmic murein tripeptide binding protein MppA, was previously reported to overproduce MarA and exhibit a multiple-antibiotic resistance (Mar) phenotype (H. Li and J. T. Park, J. Bacteriol. 181:4842-4847, 1999). We found that TP98...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.4.1465-1469.2003

    authors: Bina X,Perreten V,Levy SB

    更新日期:2003-02-01 00:00:00

  • Escherichia coli Lrp (leucine-responsive regulatory protein) does not directly regulate expression of the leu operon promoter.

    abstract::Studies by R. Lin et al. (J. Bacteriol. 174:1948-1955, 1992) suggested that the Escherichia coli leu operon might be a member of the Lrp regulon. Their results were obtained with a leucine auxotroph; in leucine prototrophs grown in a medium lacking leucine, there was little difference in leu operon expression between ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.20.6547-6551.1999

    authors: Landgraf JR,Boxer JA,Calvo JM

    更新日期:1999-10-01 00:00:00

  • Function of alpha-D-glucosyl monophosphorylpolyprenol in biosynthesis of cell wall teichoic acids in Bacillus coagulans.

    abstract::D-[alpha-14C]]glucosyl phosphorylpolyprenol ([ 14C]Glc-P-prenol) was formed from UDP-D-[14C]glucose in each of the membrane systems obtained from Bacillus coagulans AHU 1631 and AHU 1634 and two Bacillus megaterium strains. Membranes of these B. coagulans strains, which possess beta-D-glucosyl branches on the repeatin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2835-2841.1989

    authors: Shimada A,Tamatukuri J,Ito E

    更新日期:1989-05-01 00:00:00

  • Isolation and characterization of catabolite repression control mutants of Pseudomonas aeruginosa PAO.

    abstract::Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.15.4700-4706.1991

    authors: Wolff JA,MacGregor CH,Eisenberg RC,Phibbs PV Jr

    更新日期:1991-08-01 00:00:00

  • capA, a cspA-like gene that encodes a cold acclimation protein in the psychrotrophic bacterium Arthrobacter globiformis SI55.

    abstract::By use of Arthrobacter globiformis SI55, a psychrotrophic bacterium capable of growth between -5 and +32 degrees C, we cloned and sequenced capA, a gene homologous to cspA encoding the major cold shock protein in Escherichia coli. The deduced protein sequence has a high level of identity with the sequences of other Cs...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.18.5670-5676.1997

    authors: Berger F,Normand P,Potier P

    更新日期:1997-09-01 00:00:00

  • Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei.

    abstract::Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925-930. 1966.-Lactobacillus casei degrades biotin when it is present ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.4.925-930.1966

    authors: Birnbaum J,Lichstein HC

    更新日期:1966-10-01 00:00:00

  • Relationships among the O-antigen gene clusters of Salmonella enterica groups B, D1, D2, and D3.

    abstract::The O antigen is an important cell wall antigen of gram-negative bacteria, and the genes responsible for its biosynthesis are located in a gene cluster. We have cloned and sequenced the DNA segment unique to the O-antigen gene cluster of Salmonella enterica group D3. This segment includes a novel O-antigen polymerase ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.4.1002-1007.1998

    authors: Curd H,Liu D,Reeves PR

    更新日期:1998-02-01 00:00:00

  • Myxospore induction in a nondispersed growing mutant of Myxococcus xanthus.

    abstract::Myxococcus xanthus RB5, a rough-colony-forming, nondispersed growing mutant of strain FBt, forms macroscopic, multicellular masses of radially oriented cells in shake cultures. The cells appear to be held together by slime fibrils. Physical and enzymatic methods to disrupt the spheres were unsuccessful as were attempt...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.1.302-306.1975

    authors: Burchard RP

    更新日期:1975-04-01 00:00:00

  • Double-bond requirement in a fatty acid desaturase mutant of Saccharomyces cerevisiae.

    abstract::A fatty acid Delta(9)-desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Delta(9)cis double bond is necessary for growth. The need for a specific c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.1.160-165.1970

    authors: Wisnieski BJ,Keith AD,Resnick MR

    更新日期:1970-01-01 00:00:00

  • Reproducible and rapid methods for the isolation and assay of a-factor, a yeast mating hormone.

    abstract::The Saccharomyces cerevisiae mating hormone a-factor has been difficult to isolate reproducibly in sufficient yields by methods using ion-exchange chromatography, probably because of its pronounced hydrophobicity. In this work, a hydrophobic adsorbent (Amberlite XAD-2), in an insoluble bead from, was used to isolate l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.3.1153-1161.1982

    authors: Strazdis JR,MacKay VL

    更新日期:1982-09-01 00:00:00

  • Bacterial dissimilation of streptomycin.

    abstract::Klein, Dolph (Rutgers, The State University, New Brunswick, N. J.), and David Pramer. Bacterial dissimilation of streptomycin. J. Bacteriol. 82:505-510. 1961.-The influence of various nutritional and environmental factors on the dissimilation of streptomycin by a pseudomonad isolated from soil was investigated, and co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.4.505-510.1961

    authors: KLEIN D,PRAMER D

    更新日期:1961-10-01 00:00:00

  • Two paralogous families of a two-gene subtilisin operon are widely distributed in oral treponemes.

    abstract::Certain oral treponemes express a highly proteolytic phenotype and have been associated with periodontal diseases. The periodontal pathogen Treponema denticola produces dentilisin, a serine protease of the subtilisin family. The two-gene operon prcA-prtP is required for expression of active dentilisin (PrtP), a putati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.23.6860-6869.2003

    authors: Correia FF,Plummer AR,Ellen RP,Wyss C,Boches SK,Galvin JL,Paster BJ,Dewhirst FE

    更新日期:2003-12-01 00:00:00

  • Growth and division of Spiroplasma citri: elongation of elementary helices.

    abstract::The smallest viable cell of Spiroplasma citri is a two-turn helix (elementary helix). This elementary helix grows into longer parental cells, which then divide by constriction. The helical morphology is conserved during this process. The growth pattern of S. citri membranes has been investigated by different methods o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.1.23-28.1984

    authors: Garnier M,Clerc M,Bové JM

    更新日期:1984-04-01 00:00:00

  • Alanylclavam biosynthetic genes are clustered together with one group of clavulanic acid biosynthetic genes in Streptomyces clavuligerus.

    abstract::Streptomyces clavuligerus produces at least five different clavam metabolites, including clavulanic acid and the methionine antimetabolite, alanylclavam. In vitro transposon mutagenesis was used to analyze a 13-kb region upstream of the known paralogue gene cluster. The paralogue cluster includes one group of clavulan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00698-08

    authors: Zelyas NJ,Cai H,Kwong T,Jensen SE

    更新日期:2008-12-01 00:00:00

  • Conjugative transfer of Enterococcus faecalis plasmid pAD1: nucleotide sequence and transcriptional fusion analysis of a region involved in positive regulation.

    abstract::The Enterococcus faecalis plasmid pAD1 undergoes conjugative transfer in response to cAD1, a peptide sex pheromone emitted by potential bacterial recipients. Regulation of pAD1 transfer involves a number of plasmid-encoded determinants:iad, which determines a peptide-competitive inhibitor iAD1; signal sensing and tran...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.10.3152-3160.1992

    authors: Pontius LT,Clewell DB

    更新日期:1992-05-01 00:00:00

  • CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F' INCORPORATING THEM.

    abstract::Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is loca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.554-561.1964

    authors: ISHIBASHI M,SUGINO Y,HIROTA Y

    更新日期:1964-03-01 00:00:00

  • F1C fimbriae of a uropathogenic Escherichia coli strain: genetic and functional organization of the foc gene cluster and identification of minor subunits.

    abstract::The genetic organization of the foc gene cluster has been studied; six genes involved in the biogenesis of F1C fimbriae were identified. focA encodes the major fimbrial subunit, focC encodes a product that is indispensable for fimbria formation, focG and focH encode minor fimbrial subunits, and focI encodes a protein ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.1114-1120.1990

    authors: Riegman N,Kusters R,Van Veggel H,Bergmans H,Van Bergen en Henegouwen P,Hacker J,Van Die I

    更新日期:1990-02-01 00:00:00

  • Role of 3-Nitropropanoic acid in nitrate formation by Aspergillus flavus.

    abstract::Doxtader, K. G. (Cornell University, Ithaca, N.Y.), and M. Alexander. Role of 3-nitropropanoic acid in nitrate formation by Aspergillus flavus. J. Bacteriol. 91:1186-1191. 1966.-Aspergillus flavus formed nitrate, 3-nitropropanoic acid (3-NPA), kojic acid, and a substance tentatively identified as N-formyl-N-hydroxy-gl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.1186-1191.1966

    authors: Doxtader KG,Alexander M

    更新日期:1966-03-01 00:00:00

  • Growth of mycobacteria on carbon monoxide and methanol.

    abstract::Several mycobacterial strains, such as Mycobacterium flavescens, Mycobacterium gastri, Mycobacterium neoaurum, Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium vaccae, were found to grow on carbon monoxide (CO) as the sol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.1.142-147.2003

    authors: Park SW,Hwang EH,Park H,Kim JA,Heo J,Lee KH,Song T,Kim E,Ro YT,Kim SW,Kim YM

    更新日期:2003-01-01 00:00:00

  • Genome sequence of Reyranella massiliensis, a bacterium associated with amoebae.

    abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01260-12

    authors: Pagnier I,Croce O,Robert C,Raoult D,La Scola B

    更新日期:2012-10-01 00:00:00

  • Role of genetic redundancy in polyhydroxyalkanoate (PHA) polymerases in PHA biosynthesis in Rhodospirillum rubrum.

    abstract::This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polym...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01111-12

    authors: Jin H,Nikolau BJ

    更新日期:2012-10-01 00:00:00

  • FOOD-POISONING POTENTIAL OF THE ENTEROCOCCI.

    abstract::Deibel, R. H. (American Meat Institute Foundation, Chicago, Ill.), and J. H. Silliker. Food-poisoning potential of the enterococci. J. Bacteriol. 85:827-832. 1963.-A total of 23 enterococcus strains were fed to two and sometimes three human volunteers in an effort to elicit food-poisoning symptoms. Each culture was co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.4.827-832.1963

    authors: DEIBEL RH,SILLIKER JH

    更新日期:1963-04-01 00:00:00

  • Chromosomal mutation that affects excretion of hemolysin in Escherichia coli.

    abstract::Two types of mutants unable to excrete hemolysin were obtained when E. coli 5K carrying the multicopy hemolytic recombinant plasmid pANN202-312 was mutagenized with Mu d1. One type is altered in the plasmid hly-specific gene, hlyBb, but the other is caused by an insertion of Mu d1 into a chromosomal locus. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.3.1083-1085.1984

    authors: Juarez A,Goebel W

    更新日期:1984-09-01 00:00:00

  • Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene.

    abstract::Two different 30-nucleotide regions of the cryIAc insecticidal protoxin gene from Bacillus thuringiensis were randomly mutagenized. One region was within one of seven amphipathic helices believed to be important for the formation of ion channels. There was no loss of toxicity for three test insects by any of 27 mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.14.4059-4065.1995

    authors: Aronson AI,Wu D,Zhang C

    更新日期:1995-07-01 00:00:00