The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression.


:We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no more than 4 base pairs downstream from the start site of transcription, on which basis we infer that spoIVC is regulated at the level of transcription initiation. Using a transcriptional fusion of the spoIVC gene to the lacZ gene of Escherichia coli, we found that spoIVC expression was turned on at the third to fourth hour of sporulation (at about the developmental stage [IV] that its products are required in spore formation) and that this transcription was largely restricted to the mother cell chamber of the sporangium. Mutations in many different spo genes (causing blocks at stages 0 to V) were found to influence (negatively and positively) the level of spoIVC expression. Our results distinguish the mode of spoIVC regulation from that of previously studied sporulation genes and indicate that it is representative of a new regulon of mother cell-specific gene expression.


J Bacteriol


Journal of bacteriology


Kunkel B,Sandman K,Panzer S,Youngman P,Losick R




Has Abstract


1988-08-01 00:00:00












  • Isolation and characterization of the Neurospora crassa endoplasmic reticulum.

    abstract::The endoplasmic reticulum from Neurospora crassa was identified by monitoring the activity of the putative enzyme marker phosphatidylcholine glyceride transferase. After differential centrifugation of a cell homogenate, phosphatidylcholine glyceride transferase activity initially copurified with plasma membrane H+-ATP...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Borgeson CE,Bowman BJ

    更新日期:1983-10-01 00:00:00

  • The All-Alpha Domains of Coupling Proteins from the Agrobacterium tumefaciens VirB/VirD4 and Enterococcus faecalis pCF10-Encoded Type IV Secretion Systems Confer Specificity to Binding of Cognate DNA Substrates.

    abstract:UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

    更新日期:2015-07-01 00:00:00

  • Biosynthesis of the outer membrane receptor for vitamin B12, E colicins, and bacteriophage BF23 by Escherichia coli: kinetics of phenotypic expression after the introduction of bfe+ and bfe alleles.

    abstract::The bfe locus codes for the cell surface receptor for vitamin B12, the E colicins, and bacteriophage BF23 in the Escherichia coli outer membrane. When the bfe+ allele, which is closely linked to the argH locus, was introduced into an argH bfe recipient by conjugation, arg+ recombinant cells rapidly and simultaneously ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bassford PJ Jr,Kadner RJ,Schnaitman CA

    更新日期:1977-01-01 00:00:00

  • Opines stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti plasmid.

    abstract::Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Veluthambi K,Krishnan M,Gould JH,Smith RH,Gelvin SB

    更新日期:1989-07-01 00:00:00

  • Possible involvement of the division cycle in dispersal of Escherichia coli from biofilms.

    abstract::Growth rate control of adherent, sessile populations was achieved by the controlled perfusion of membrane-associated bacterial biofilms by the method of Gilbert et al. (P. Gilbert, D. G. Allison, D. J. Evans, P. S. Handley, and M. R. W. Brown, Appl. Environ. Microbiol. 55:1308-1311, 1989). Changes in cell surface hydr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Allison DG,Evans DJ,Brown MR,Gilbert P

    更新日期:1990-03-01 00:00:00

  • Ethanol production by thermophilic bacteria: metabolic control of end product formation in Thermoanaerobium brockii.

    abstract::Specific changes in the chemical and microbial composition of Thermoanaerobium brockii fermentations were compared and related to alterations of process rates, end product yields, and growth parameters. Fermentation of starch as compared with glucose was associated with significant decreases in growth rate and intrace...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ben-Bassat A,Lamed R,Zeikus JG

    更新日期:1981-04-01 00:00:00

  • Crystal structure of the cystic fibrosis transmembrane conductance regulator inhibitory factor Cif reveals novel active-site features of an epoxide hydrolase virulence factor.

    abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

    更新日期:2010-04-01 00:00:00

  • Genome sequence of Lactococcus garvieae 8831, isolated from rainbow trout lactococcosis outbreaks in Spain.

    abstract::Lactococcus garvieae is the etiological agent of lactococcosis, one of the most important disease threats to the sustainability of the rainbow trout farming industry. Here, we present the draft genome sequence of Lactococcus garvieae strain 8831, isolated from diseased rainbow trout, which is composed of 2,087,276 bp ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Aguado-Urda M,López-Campos GH,Gibello A,Cutuli MT,López-Alonso V,Fernández-Garayzábal JF,Blanco MM

    更新日期:2011-08-01 00:00:00

  • Dual control of sbo-alb operon expression by the Spo0 and ResDE systems of signal transduction under anaerobic conditions in Bacillus subtilis.

    abstract::The Bacillus subtilis sbo-alb operon contains sboA, the structural gene for the bacteriocin subtilosin, and the alb genes required for subtilosin production. Transcription from the sbo-alb promoter is highly induced by oxygen limitation. The transcriptional regulation of the sbo-alb operon is under dual control involv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nakano MM,Zheng G,Zuber P

    更新日期:2000-06-01 00:00:00

  • Polymorphisms in inc proteins and differential expression of inc genes among Chlamydia trachomatis strains correlate with invasiveness and tropism of lymphogranuloma venereum isolates.

    abstract::Chlamydia trachomatis is a human bacterial pathogen that multiplies only within an intracellular membrane-bound vacuole, the inclusion. C. trachomatis includes ocular and urogenital strains, usually causing infections restricted to epithelial cells of the conjunctiva and genital mucosa, respectively, and lymphogranulo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Almeida F,Borges V,Ferreira R,Borrego MJ,Gomes JP,Mota LJ

    更新日期:2012-12-01 00:00:00

  • Direct quantitation of the numbers of individual penicillin-binding proteins per cell in Staphylococcus aureus.

    abstract::The penicillin-binding proteins (PBPs) are a set of enzymes that participate in bacterial peptidoglycan assembly. The absolute numbers of each PBP were determined by direct measurement and have been reported for two Staphylococcus aureus strains, RN4220 (methicillin-sensitive S. aureus) and RN450M (methicillin-resista...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pucci MJ,Dougherty TJ

    更新日期:2002-01-01 00:00:00

  • Isolation and expansion of the catabolic potential of a Pseudomonas putida strain able to grow in the presence of high concentrations of aromatic hydrocarbons.

    abstract::Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole C source. The strain was able to grow in the presence of 90% (vol/vol) toluene and was tolerant to organic solvents whose log P(ow) (octanol/water partition coefficient) was higher than 2.3. Solvent toleranc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ramos JL,Duque E,Huertas MJ,Haïdour A

    更新日期:1995-07-01 00:00:00

  • SigmaS controls multiple pathways associated with intracellular multiplication of Legionella pneumophila.

    abstract::Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hovel-Miner G,Pampou S,Faucher SP,Clarke M,Morozova I,Morozov P,Russo JJ,Shuman HA,Kalachikov S

    更新日期:2009-04-01 00:00:00

  • osmY, a new hyperosmotically inducible gene, encodes a periplasmic protein in Escherichia coli.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yim HH,Villarejo M

    更新日期:1992-06-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Leduc M,Frehel C,van Heijenoort J

    更新日期:1985-02-01 00:00:00

  • Bacteriophage T4 self-assembly: in vitro reconstitution of recombinant gp2 into infectious phage.

    abstract::T4 gene 2 mutants have a pleiotropic phenotype: degradation of injected phage DNA by exonuclease V (ExoV) in the recBCD(+) host cell cytoplasm and a low burst size due, at least in part, to a decreased ability for head-to-tail (H-T) joining. The more N terminal the mutation, the more pronounced is the H-T joining defe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang GR,Vianelli A,Goldberg EB

    更新日期:2000-02-01 00:00:00

  • The mere lack of rT modification in initiator tRNA does not facilitate formylation-independent initiation in Escherichia coli.

    abstract::Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Thanedar S,Dineshkumar TK,Varshney U

    更新日期:2001-12-01 00:00:00

  • Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins.

    abstract::Type VI secretion systems (T6SS) are bacteriophage-derived macromolecular machines responsible for the release of at least two proteins in the milieu, which are thought to form an extracellular appendage. Although several T6SS have been shown to be involved in the virulence of animal and plant pathogens, clusters enco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bernard CS,Brunet YR,Gavioli M,Lloubès R,Cascales E

    更新日期:2011-05-01 00:00:00

  • Suppressed nonsense mutations in the araC gene of Escherichia coli provide three novel variant proteins.

    abstract::A total of 400 suppressible mutations have been isolated in the araC gene of Escherichia coli. Based on deletion mapping, growth patterns when suppressed, and intragenic recombination, 37 mutants have been determined to contain unique mutations. Rapid plate assays were developed to test for each of the three AraC prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schecter JB

    更新日期:1983-06-01 00:00:00

  • Spermidine requirement for Bacillus thuringiensis ribosomes in cell-free phenylalanine incorporation.

    abstract::A cell-free system from Bacillus thuringiensis was found to actively incorporate phenylalanine into hot trichloroacetic acid-precipitable material in the presence of synthetic polynucleotide, ribosomes, S-100 supernatant, an energy-generating system, and guanosine triphosphate. Phenylalanine incorporation was absolute...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Changchien LM,Aronson JN

    更新日期:1970-09-01 00:00:00

  • Assignment of tra cistrons to EcoRI fragments of F sex factor DNA.

    abstract::We describe here the cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector. These plasmids, as well as the series of Skurray et al. (Proc. Natl. Acad. Sci. U.S.A. 73:64-68, 1976), have been used to refine the map positions of tra cistrons on the F factor as well as to define a new D...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Achtman M,Skurray RA,Thompson R,Helmuth R,Hall S,Beutin L,Clark AJ

    更新日期:1978-03-01 00:00:00

  • EbfC (YbaB) is a new type of bacterial nucleoid-associated protein and a global regulator of gene expression in the Lyme disease spirochete.

    abstract::Nearly every known species of Eubacteria encodes a homolog of the Borrelia burgdorferi EbfC DNA-binding protein. We now demonstrate that fluorescently tagged EbfC associates with B. burgdorferi nucleoids in vivo and that chromatin immunoprecipitation (ChIP) of wild-type EbfC showed it to bind in vivo to sites througho...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jutras BL,Bowman A,Brissette CA,Adams CA,Verma A,Chenail AM,Stevenson B

    更新日期:2012-07-01 00:00:00

  • Purification of penicillin-binding protein 2 of Escherichia coli.

    abstract::Penicillin-binding protein 2 (PBP-2) of Escherichia coli K-12 was purified by covalent affinity chromatography using 6-aminopenicillanic acid covalently coupled to carboxymethyl-Sepharose (6-APA-CM-Sepharose). Purification of PBP-2 was accomplished by prebinding the methoxy cephalosporin, cefoxitin, to the Triton X-10...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Curtis SJ,Strominger JL

    更新日期:1981-01-01 00:00:00

  • Control of mucoidy in Pseudomonas aeruginosa: transcriptional regulation of algR and identification of the second regulatory gene, algQ.

    abstract::A new alginate regulatory gene, algQ, was identified in a chromosomal region which, when tandemly amplified, induces mucoidy in Pseudomonas aeruginosa. The algQ gene was found closely linked to the previously identified algR gene. Both algQ and algR were required for transcription of the key alginate biosynthetic gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Deretic V,Konyecsni WM

    更新日期:1989-07-01 00:00:00


    abstract::Niederpruem, Donald J. (Indiana University Medical Center, Indianapolis). Respiration of basidiospores of Schizophyllum commune. J. Bacteriol. 88:210-215. 1964.-The aerobic metabolism of basidiospores of the wood-rotting mushroom Schizophyllum commune was investigated by use of manometric techniques and specific respi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: NIEDERPRUEM DJ

    更新日期:1964-07-01 00:00:00

  • Transcriptomic analysis of the sulfate starvation response of Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tralau T,Vuilleumier S,Thibault C,Campbell BJ,Hart CA,Kertesz MA

    更新日期:2007-10-01 00:00:00

  • Growth of mycobacteria on carbon monoxide and methanol.

    abstract::Several mycobacterial strains, such as Mycobacterium flavescens, Mycobacterium gastri, Mycobacterium neoaurum, Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium vaccae, were found to grow on carbon monoxide (CO) as the sol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Park SW,Hwang EH,Park H,Kim JA,Heo J,Lee KH,Song T,Kim E,Ro YT,Kim SW,Kim YM

    更新日期:2003-01-01 00:00:00

  • Induction and repression of nitrate reductase in Neurospora crassa.

    abstract::Synthesis of wild-type Neurospora crassa assimilatory nitrate reductase is induced in the presence of nitrate ions and repressed in the presence of ammonium ions. Effects of several Neurospora mutations on the regulation of this enzyme are shown: (i) the mutants, nit-1 and nit-3, involving separate lesions, lack reduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dantzig AH,Zurowski WK,Ball TM,Nason A

    更新日期:1978-02-01 00:00:00

  • Suppression of an Hsp70 mutant phenotype in Saccharomyces cerevisiae through loss of function of the chromatin component Sin1p/Spt2p.

    abstract::The Ssa subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded by SSA1, SSA2, SSA3, and SSA4. Deletion of the two constitutively expressed genes, SSA1 and SSA2, results in cells which are slow growing and temperature sensitive. In this study, we demonstrate that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Baxter BK,Craig EA

    更新日期:1998-12-01 00:00:00

  • Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis.

    abstract::Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Popham DL,Meador-Parton J,Costello CE,Setlow P

    更新日期:1999-10-01 00:00:00