Augmented expression of polysaccharide intercellular adhesin in a defined Staphylococcus epidermidis mutant with the small-colony-variant phenotype.

abstract：:Protein and ribonucleic acid (RNA) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. Immediately after heating, cells were incubated in growth media containing either [14C]leucine or [3H]uracil. The labeled cells were subsequently treated with pen...

journal_title：Journal of bacteriology

authors： Dean RG,McGroarty EJ

更新日期：1979-05-01 00:00:00

abstract：:In untreated cells of the marine pseudomonad studied here, alkaline phosphatase was found to be located in the periplasmic space, at the cell surface, and in the medium into which it had been shed during growth. Washing in 0.5 M NaCl, which removed the loosely bound outer layer, caused a shift of periplasmic enzyme to...

journal_title：Journal of bacteriology

authors： Costerton JW

更新日期：1973-06-01 00:00:00

abstract：UNLABELLED:The Pseudomonas aeruginosa cyclic AMP (cAMP)-Vfr system (CVS) is a global regulator of virulence gene expression. Regulatory targets include type IV pili, secreted proteases, and the type III secretion system (T3SS). The mechanism by which CVS regulates T3SS gene expression remains undefined. Single-cell exp...

journal_title：Journal of bacteriology

authors： Marsden AE,Intile PJ,Schulmeyer KH,Simmons-Patterson ER,Urbanowski ML,Wolfgang MC,Yahr TL

更新日期：2016-04-14 00:00:00

abstract：:Gerhardt, Philipp (University of Michigan, Ann Arbor) and S. H. Black. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J. Bacteriol. 82:750-760. 1961.-More than 100 compounds were tested for their uptake by dormant spores of a bacillus. The extent of penetration was found to be d...

journal_title：Journal of bacteriology

authors： GERHARDT P,BLACK SH

更新日期：1961-11-01 00:00:00

abstract：:Rabbits immunized with mucopeptide derived from cell wall debris of Streptococcus agalactiae by use of the formamide extraction technique developed specific antibodies for bovine heart, skeletal muscle, lymph nodes, and blood buffy coat extracts. ...

journal_title：Journal of bacteriology

authors： Dodd K,Norcross NL

更新日期：1967-02-01 00:00:00

abstract：:In many microorganisms the first step for alkane degradation is the terminal oxidation of the molecule by an alkane hydroxylase. We report the characterization of a gene coding for an alkane hydroxylase in a Burkholderia cepacia strain isolated from an oil-contaminated site. The protein encoded showed similarity to ot...

journal_title：Journal of bacteriology

authors： Marín MM,Smits TH,van Beilen JB,Rojo F

更新日期：2001-07-01 00:00:00

abstract：:Recombinant plasmid pNP5, consisting of plasmid pMB9 on which the uvrB gene is cloned, fully complements for the defects due to chromosomal uvrB mutations in the presence of the amber suppressor sup-6 or supF. Correndonuclease II activity was also completely restored in in UvrB strains containing both plasmid pNP5 and...

journal_title：Journal of bacteriology

authors： Pannekoek H,Noordermeer I,van de Putte P

更新日期：1979-07-01 00:00:00

abstract：:Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...

journal_title：Journal of bacteriology

authors： Zhang-Barber L,Turner AK,Martin G,Frankel G,Dougan G,Barrow PA

更新日期：1997-11-01 00:00:00

abstract：:The ftsZ gene is thought to be an essential cell division gene in Escherichia coli. We constructed a null allele of ftsZ in a strain carrying additional copies of ftsZ on a plasmid with a temperature-sensitive replication defect. This strain was temperature sensitive for cell division and viability, confirming that ft...

journal_title：Journal of bacteriology

authors： Dai K,Lutkenhaus J

更新日期：1991-06-01 00:00:00

abstract：:The ruvB genes of the highly divergent thermophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expressed in Escherichia coli. Both thermostable RuvB proteins were purified to homogeneity. Like E. coli RuvB protein, both purified thermostable RuvB proteins showed strong double-s...

journal_title：Journal of bacteriology

authors： Tong J,Wetmur JG

更新日期：1996-05-01 00:00:00

abstract：:Proline dehydrogenase (PutA) is a bifunctional enzyme that catalyzes the oxidation of proline to glutamate. In Sinorhizobium meliloti, as in other microorganisms, the putA gene is transcriptionally activated in response to proline. In Rhodobacter capsulatus, Agrobacterium, and most probably in Bradyrhizobium, this act...

journal_title：Journal of bacteriology

authors： Soto MJ,Jiménez-Zurdo JI,van Dillewijn P,Toro N

更新日期：2000-04-01 00:00:00

abstract：:Escherichia coli ssrA encodes a small stable RNA molecule, tmRNA, that has many diverse functions, including tagging abnormal proteins for degradation, supporting phage growth, and modulating the activity of DNA binding proteins. Here we show that ssrA plays a role in Salmonella enterica serovar Typhimurium pathogenes...

journal_title：Journal of bacteriology

authors： Julio SM,Heithoff DM,Mahan MJ

更新日期：2000-03-01 00:00:00

abstract：:Salmonella typhimurium LT2 excreted under certain conditions an antibiotic substance designated typhimuricin. It is suggested that the LT2 "cryptic" plasmid is involved in its production and in the immunity to it. Preliminary characterization of typhimuricin is presented. ...

journal_title：Journal of bacteriology

authors： Ben-Gurion R,Flashner Y

更新日期：1982-10-01 00:00:00

abstract：:Lactobacillus fructivorans is important in the generation of particular flavors and in other ripening processes associated with fermented food. Here, we present the draft genome sequence of the type strain Lactobacillus fructivorans KCTC 3543 (1,373,326 bp, with a G+C content of 38.9%), which consists of 5 scaffolds. ...

journal_title：Journal of bacteriology

authors： Nam SH,Choi SH,Kang A,Lee KS,Kim DW,Kim RN,Kim DS,Park HS

更新日期：2012-04-01 00:00:00

abstract：:Gene sequences encoding the enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from many bacterial sources were analyzed. It was shown that whereas gram-negative bacteria have only one murA gene, gram-positive bacteria have two distinct genes encoding these enzymes which have possibly arisen from gene dupl...

journal_title：Journal of bacteriology

authors： Du W,Brown JR,Sylvester DR,Huang J,Chalker AF,So CY,Holmes DJ,Payne DJ,Wallis NG

更新日期：2000-08-01 00:00:00

abstract：:Bacterial cell division is a complex process that relies on a multiprotein complex composed of a core of widely conserved and generally essential proteins and on accessory proteins that vary in number and identity in different bacteria. The assembly of this complex and, particularly, the initiation of constriction are...

journal_title：Journal of bacteriology

authors： Osorio A,Camarena L,Cevallos MA,Poggio S

更新日期：2017-03-28 00:00:00

abstract：:The 2,760-base-pair (bp) PstI-EcoRI segment of the chromosome of Pseudomonas aeruginosa PA103 which carries the exotoxin A structural gene was expressed from an internal promoter when cloned in a pUC9 derivative and transformed into a nontoxigenic mutant of P. aeruginosa PAO1. The unique terminal EcoRI site was delete...

journal_title：Journal of bacteriology

authors： Tsaur ML,Clowes RC

更新日期：1989-05-01 00:00:00

abstract：:A temperature-sensitive Schizosaccharomyces pombe mutant, cdc16-116, has been isolated which undergoes uncontrolled septation during its cell division cycle. The mutant accumulates two types of cells after 3 h of growth at the restrictive temperature: (i) type I cells (85% of the population), which complete nuclear di...

journal_title：Journal of bacteriology

authors： Minet M,Nurse P,Thuriaux P,Mitchison JM

更新日期：1979-01-01 00:00:00

abstract：:A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the molecular level. Nucleotide sequence determination revealed the presence of two open reading frames. Both open reading frames were found by deletion and complementation analysis to be necessary for expression of the he...

journal_title：Journal of bacteriology

authors： Gilmore MS,Cruz-Rodz AL,Leimeister-Wächter M,Kreft J,Goebel W

更新日期：1989-02-01 00:00:00

abstract：:After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...

journal_title：Journal of bacteriology

authors： Friedman SM,Axel R,Weinstein IB

更新日期：1967-05-01 00:00:00

abstract：:Broad-host-range plasmids and cloning vectors were conjugatively transferred to Acetobacter xylinum. One of the plasmids, RP4::Mu cts61, was used for the insertion of Tn1 into the 16-, 44-, and 64-kilobase-pair plasmids of A. xylinum. The Tn1-labeled plasmids could be mobilized by a helper plasmid. Many of the Tn1 ins...

journal_title：Journal of bacteriology

authors： Valla S,Coucheron DH,Kjosbakken J

更新日期：1986-01-01 00:00:00

abstract：:The temperature-sensitive cyr1-2 mutant in Saccharomyces cerevisiae produces low levels of adenylate cyclase and cyclic AMP at 25 degrees C and is unable to synthesize repressible acid phosphatase at 25 degrees C. Suppressor mutants of cyr1-2 were isolated by detecting acid phosphatase activity. One of the dominant su...

journal_title：Journal of bacteriology

authors： Morishita T,Uno I

更新日期：1991-04-01 00:00:00

abstract：:The PDC1 gene coding for a pyruvate decarboxylase (PDC; EC 4.1.1.1) was deleted from the Saccharomyces cerevisiae genome. The resulting pdc1(0) mutants were able to grow on glucose and still contained 60 to 70% of the wild-type PDC activity. Two DNA fragments with sequences homologous to that of the PDC1 gene were clo...

journal_title：Journal of bacteriology

authors： Seeboth PG,Bohnsack K,Hollenberg CP

更新日期：1990-02-01 00:00:00

abstract：:The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...

journal_title：Journal of bacteriology

authors： Shimoda E,Muto T,Horiuchi T,Furuya N,Komano T

更新日期：2008-02-01 00:00:00

abstract：:Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type w...

journal_title：Journal of bacteriology

authors： Scott WA

更新日期：1977-06-01 00:00:00

abstract：:A novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important poly...

journal_title：Journal of bacteriology

authors： Sabirova JS,Ferrer M,Lünsdorf H,Wray V,Kalscheuer R,Steinbüchel A,Timmis KN,Golyshin PN

更新日期：2006-12-01 00:00:00

abstract：:A modified 3-hydroxypropionate cycle has been proposed as the autotrophic CO2 fixation pathway for the thermoacidophilic crenarchaeon Metallosphaera sedula. The cycle requires the reductive conversion of 3-hydroxypropionate to propionyl-coenzyme A (propionyl-CoA). The specific activity of the 3-hydroxypropionate-, CoA...

journal_title：Journal of bacteriology

authors： Alber BE,Kung JW,Fuchs G

更新日期：2008-02-01 00:00:00

abstract：:A compound, isolated from the culture fluid of a nadC auxotroph of Escherichia coli grown in a minimal medium, supports the growth of both a nadA and a nadB mutant. This metabolite exhibits an ultraviolet light absorption spectrum and a mass spectrum, different from quinolinic acid. This compound may be the precursor ...

journal_title：Journal of bacteriology

authors： Chen JL,Tritz GJ

更新日期：1975-01-01 00:00:00

abstract：:Two isoleucine analogues and two leucine analogues were examined for their ability to replace the natural amino acid preventing the accumulation of threonine deaminase-forming potential. The procedure used to study repression by the analogues distinguishes between true repression and the formation of inactive enzyme b...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1973-11-01 00:00:00

abstract：:The oral bacterium Streptococcus salivarius takes up lactose via a transporter called LacS that shares 95% identity with the LacS from Streptococcus thermophilus, a phylogenetically closely related organism. S. thermophilus releases galactose into the medium during growth on lactose. Expulsion of galactose is mediated...

journal_title：Journal of bacteriology

authors： Lessard C,Cochu A,Lemay JD,Roy D,Vaillancourt K,Frenette M,Moineau S,Vadeboncoeur C

更新日期：2003-12-01 00:00:00