Cloning and nucleotide sequence analysis of the colH gene from Clostridium histolyticum encoding a collagenase and a gelatinase.

abstract：:A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for...

journal_title：Journal of bacteriology

authors： Smith CJ

更新日期：1985-10-01 00:00:00

abstract：:Hirota, Yukinori (University of Osaka, Osaka, Japan), Toshio Fujii, and Yukinobu Nishimura. Loss and repair of conjugal fertility and infectivity of the resistance factor and sex factor in Escherichia coli. J. Bacteriol. 91:1298-1304. 1966.-The drug-resistance factor, R, and the sex factor, F, have homologous traits, ...

journal_title：Journal of bacteriology

authors： Hirota Y,Fujii T,Nishimua Y

更新日期：1966-03-01 00:00:00

abstract：:Mutants deficient in the production of bacteriochlorophyll c (Bchl c) and one mutant lacking colored carotenoids were isolated from the filamentous gliding bacterium Chloroflexus aurantiacus. Mutagenesis was achieved by using UV radiation or N-methyl-N'-nitro-N-nitrosoguanidine. Several clones were isolated that were ...

journal_title：Journal of bacteriology

authors： Pierson BK,Keith LM,Leovy JG

更新日期：1984-07-01 00:00:00

abstract：:In Escherichia coli, the three branched-chain amino acid activating enzymes appear to be essential for multivalent repression of the isoleucine- and valine-forming enzymes. The results of experiments with a mutant, strain CU18, having an altered threonine deaminase, indicate that free isoleucine and some form of threo...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1974-01-01 00:00:00

abstract：:The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...

journal_title：Journal of bacteriology

authors： Mukerjea R,Ghosh P

更新日期：2013-10-01 00:00:00

abstract：:Bacillus subtilis responds to phosphate starvation stress by inducing the PhoP and SigB regulons. While the PhoP regulon provides a specific response to phosphate starvation stress, maximizing the acquisition of phosphate (P(i)) from the environment and reducing the cellular requirement for this essential nutrient, th...

journal_title：Journal of bacteriology

authors： Allenby NE,O'Connor N,Prágai Z,Ward AC,Wipat A,Harwood CR

更新日期：2005-12-01 00:00:00

abstract：:Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...

journal_title：Journal of bacteriology

authors： Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KA

更新日期：2008-03-01 00:00:00

abstract：:Rhodococcus sp. strain DK17 is capable of utilizing various derivatives of benzene and bicyclics containing both aromatic and alicyclic moieties as sole carbon and energy sources. Here, we present the 9,107,362-bp draft genome sequence of DK17 and its genomic analysis in comparison with other members of the genus Rhod...

journal_title：Journal of bacteriology

authors： Yoo M,Kim D,Choi KY,Chae JC,Zylstra GJ,Kim E

更新日期：2012-08-01 00:00:00

abstract：:We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mech...

journal_title：Journal of bacteriology

authors： Prudhomme M,Turlan C,Claverys JP,Chandler M

更新日期：2002-01-01 00:00:00

abstract：:A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were prev...

journal_title：Journal of bacteriology

authors： Freedman HH,Fox AE,Willis RS,Schwartz BS

更新日期：1968-02-01 00:00:00

abstract：:In contrast to previous findings, we demonstrate that the dissimilatory (bi)sulfite reductase genes (dsrAB) of Desulfobacula toluolica were vertically inherited. Furthermore, Desulfobacterium anilini and strain mXyS1 were identified, by dsrAB sequencing of 17 reference strains, as members of the donor lineage for thos...

journal_title：Journal of bacteriology

authors： Zverlov V,Klein M,Lücker S,Friedrich MW,Kellermann J,Stahl DA,Loy A,Wagner M

更新日期：2005-03-01 00:00:00

abstract：:Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...

journal_title：Journal of bacteriology

authors： Huyer M,Page WJ

更新日期：1989-07-01 00:00:00

abstract：:Plasmid Rms312, specifying resistance to tetracycline (Tc), chloramphenicol (Cm), streptomycin (Sm), sulfonamide (Su), and mercury chloride (Mer), deletes both Tc and Cm Sm Su Mer determinants at a high frequency in Salmonella typhimurium LT2. S. typhimurium mutants that were stable carriers of Rms312 were isolated by...

journal_title：Journal of bacteriology

authors： Watanabe H,Hashimoto H,Mitsuhashi S

更新日期：1980-04-01 00:00:00

abstract：:Con minus mutants, conjugation-defective mutants of Escherichia coli K-12, have been shown to be tolerant to colicins K and L. They map at approximately 14 min on the genetic map. The significance of the colicin tolerance of conjugation-defective mutants is discussed. ...

journal_title：Journal of bacteriology

authors： Davies JK,Reeves P

更新日期：1975-07-01 00:00:00

abstract：:Eubacterium sp. strain VPI 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. At least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. One of these, of molecular weight 27,000 (P-27), was shown to be involved in the 7-de...

journal_title：Journal of bacteriology

authors： Coleman JP,White WB,Lijewski M,Hylemon PB

更新日期：1988-05-01 00:00:00

abstract：:The anaerobic pathogen Clostridium perfringens encodes either toxin genes or antibiotic resistance determinants on a unique family of conjugative plasmids that have a novel conjugation region, the tcp locus. Studies of the paradigm conjugative plasmid from C. perfringens, the 47-kb tetracycline resistance plasmid pCW3...

journal_title：Journal of bacteriology

authors： Wisniewski JA,Teng WL,Bannam TL,Rood JI

更新日期：2015-02-15 00:00:00

abstract：:Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproduc...

journal_title：Journal of bacteriology

authors： Engelberg D,Mimran A,Martinetto H,Otto J,Simchen G,Karin M,Fink GR

更新日期：1998-08-01 00:00:00

abstract：:Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene). Twenty MRSP and 2 l...

journal_title：Journal of bacteriology

authors： Ralph D,McClelland M,Welsh J,Baranton G,Perolat P

更新日期：1993-02-01 00:00:00

abstract：:During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores i...

journal_title：Journal of bacteriology

authors： Wesp A,Prinz S,Fink GR

更新日期：2001-04-01 00:00:00

abstract：:A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynami...

journal_title：Journal of bacteriology

authors： McKinney J,Guerrier-Takada C,Galán J,Altman S

更新日期：2002-11-01 00:00:00

abstract：:Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not been elucidated. In this study, we found that motility of B. burgdorferi required an environment similar to interstitial ...

journal_title：Journal of bacteriology

authors： Shi W,Yang Z,Geng Y,Wolinsky LE,Lovett MA

更新日期：1998-01-01 00:00:00

abstract：:Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number...

journal_title：Journal of bacteriology

authors： Sun LV,Foster JM,Tzertzinis G,Ono M,Bandi C,Slatko BE,O'Neill SL

更新日期：2001-04-01 00:00:00

abstract：:ComX and CSF are Bacillus subtilis extracellular signaling peptides. Many different strains of B. subtilis do not communicate due to strain-specific variation of ComX. We demonstrate that CSF is a species-specific signaling molecule that partially compensates for the lack of ComX-mediated communication between differe...

journal_title：Journal of bacteriology

authors： Pottathil M,Jung A,Lazazzera BA

更新日期：2008-06-01 00:00:00

abstract：:ZmaR is a resistance determinant of unusual abundance in the environment and confers on gram-positive and gram-negative bacteria resistance to zwittermicin A, a novel broad-spectrum antibiotic produced by species of Bacillus. The ZmaR protein has no sequence similarity to proteins of known function; thus, the purpose ...

journal_title：Journal of bacteriology

authors： Stohl EA,Brady SF,Clardy J,Handelsman J

更新日期：1999-09-01 00:00:00

abstract：:We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no m...

journal_title：Journal of bacteriology

authors： Kunkel B,Sandman K,Panzer S,Youngman P,Losick R

更新日期：1988-08-01 00:00:00

abstract：:A 7.5-kbp fragment of chromosomal DNA downstream of the Vibrio cholerae vibriobactin outer membrane receptor, viuA, and the vibriobactin utilization gene, viuB, was recovered from a Sau3A lambda library of O395 chromosomal DNA. By analogy with the genetic organization of the Escherichia coli enterobactin gene cluster,...

journal_title：Journal of bacteriology

authors： Butterton JR,Choi MH,Watnick PI,Carroll PA,Calderwood SB

更新日期：2000-03-01 00:00:00

abstract：:Supplementation of the growth medium with high concentrations of NaCl, KCl, or sucrose caused a drastic change in the ratio of the two peptidoglycan-associated major outer membrane proteins of Escherichia coli K-12 in that the amounts of proteins b and c present in cell envelope preparations decreased and increased, r...

journal_title：Journal of bacteriology

authors： Alphen WV,Lugtenberg B

更新日期：1977-08-01 00:00:00

abstract：:Cell division in Caulobacter crescentus involves constriction and fission of the inner membrane (IM) followed about 20 min later by fission of the outer membrane (OM) and daughter cell separation. In contrast to Escherichia coli, the Caulobacter Tol-Pal complex is essential. Cryo-electron microscopy images of the Caul...

journal_title：Journal of bacteriology

authors： Yeh YC,Comolli LR,Downing KH,Shapiro L,McAdams HH

更新日期：2010-10-01 00:00:00

abstract：:Transport systems for K+ in Escherichia coli are not detectable in membrane vesicles, but vesicles will take up K+ (and Rb+) in the presence of valinomycin. It is generally believed that valinomycin acts as a lipid-soluble cation carrier and that it does not interact with or activate cation transport systems. This vie...

journal_title：Journal of bacteriology

authors： Altendorf K,Epstein W,Löhmann A

更新日期：1986-04-01 00:00:00

abstract：:The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...

journal_title：Journal of bacteriology

authors： Beck C,von Meyenburg HK

更新日期：1968-08-01 00:00:00