Abstract:
:We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mechanism in which an IS256 belonging to one Tn4001 copy attacks an IS256 end carried by the second Tn4001 copy. The resulting structures carry abutted left (inverted left repeat [IRL]) and right (inverted right repeat [IRR]) IS256 ends. Examination of the junction sequence suggested that it may form a relatively good promoter capable of driving transposase synthesis in Escherichia coli. This behavior resembles that of an increasing number of bacterial insertion sequences which generate integrative junctions as part of the transposition cycle. Sequence analysis of the IRL-IRR junctions demonstrated that attack of one end by the other is largely oriented (IRL attacks IRR). Our experiments also defined the functional tips of IS256 as the tips predicted from sequence alignments, confirming that the terminal 4 bp at each end are indeed different. The appearance of these multiple plasmid and transposon forms indicates that care should be exercised when Tn4001 is used in transposition mutagenesis. This is especially true when it is used with naturally transformable hosts, such as Streptococcus pneumoniae, in which reconstitution of the donor plasmid may select for higher-order multimers.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Prudhomme M,Turlan C,Claverys JP,Chandler Mdoi
10.1128/jb.184.2.433-443.2002keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
433-43issue
2eissn
0021-9193issn
1098-5530journal_volume
184pub_type
杂志文章abstract::Phage KB1 has gained use as a generalized transducing phage for Salmonella typhimurium, including strains resistant to phage P22. Integration-defective mutants of KB1 have now been isolated; one of these, int-1, is recommended for transduction when nonlysogenic recombinants are desired. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.2.907-908.1974
更新日期:1974-02-01 00:00:00
abstract::A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6864-6875.2004
更新日期:2004-10-01 00:00:00
abstract::Freeman, B. M. (Houghton Poultry Research Station, Huntingdon, England), and L. G. Chubb. Effect of Salmonella gallinarum on certain Krebs cycle intermediates of domestic fowl. J. Bacteriol. 88:93-95. 1964.-In chickens infected with Salmonella gallinarum, the citric acid content of the blood rose but that of the liver...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.93-95.1964
更新日期:1964-07-01 00:00:00
abstract::Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene cons...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.5177-5180.1994
更新日期:1994-08-01 00:00:00
abstract::The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.426-433.1982
更新日期:1982-02-01 00:00:00
abstract::The reduction of bisulfite by Desulfovibrio vulgaris was investigated. Crude extracts reduced bisulfite to sulfide without the formation (detection) of any intermediates such as trithionate or thiosulfate. When the particulate fractions was removed from crude extracts by high-speed centrifugation, the soluble supernat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.916-923.1978
更新日期:1978-12-01 00:00:00
abstract::narK mutants of Escherichia coli produce wild-type levels of nitrate reductase but, unlike the wild-type strain, do not accumulate nitrite when grown anaerobically on a glucose-nitrate medium. Comparison of the rates of nitrate and nitrite metabolism in cultures growing anaerobically on glucose-nitrate medium revealed...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.11.3303-3310.1991
更新日期:1991-06-01 00:00:00
abstract::Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.1.107-118.1998
更新日期:1998-01-01 00:00:00
abstract::This is the first complete genome sequence of the Enterobacter aerogenes species. Here we present the genome sequence of E. aerogenes KCTC 2190, which contains 5,280,350 bp with a G + C content of 54.8 mol%, 4,912 protein-coding genes, and 109 structural RNAs. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00028-12
更新日期:2012-05-01 00:00:00
abstract::A series of mutations of the P1 plasmid prophage that lead to increased copy number was isolated and analyzed. The copy number of the mutants was elevated at least five- to eightfold relative to wild-type P1, as determined by single-cell resistance to antibiotics, activity of enzymes, content of superhelical DNA, and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.3.1329-1339.1982
更新日期:1982-06-01 00:00:00
abstract::We recently demonstrated that Pseudomonas aeruginosa PAO1 undergoes a pronounced phenotypic change when introduced into the intestines of rats during surgical injury. Recovered strains displayed a specific phenotype (termed the P2 phenotype) characterized by altered pyocyanin production, high collagenase activity, hig...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01050-13
更新日期:2014-01-01 00:00:00
abstract::Two proteins that bind penicillin were observed in Escherichia coli infected with lambda phages 141, 142, 650, and 651 from the Kohara genomic library. These phages carry chromosomal DNA fragments that do not contain any known penicillin binding protein (PBP) genes, indicating that unrecognized gene products were exhi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.19.6112-6121.1997
更新日期:1997-10-01 00:00:00
abstract::We have used transcriptional and translational fusions between various vir gene promoters and the lacZ gene to study the regulation of vir genes. Like other vir promoters, the virA promoter was induced by acetosyringone in a virA virG-dependent fashion. In addition to being induced by acetosyringone, the virG promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4047-4054.1988
更新日期:1988-09-01 00:00:00
abstract::Trent, Dennis W. (University of Oklahoma School of Medicine, Oklahoma City) and L. Vernon Scott. Colorado tick fever virus in cell culture. I. Cell-type susceptibility and interaction with L cells. J. Bacteriol. 88:702-708. 1964.-Colorado tick fever (CTF) virus was serially propagated in monolayer cultures of L and FL...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.702-708.1964
更新日期:1964-09-01 00:00:00
abstract::Gaffar, Abdul (Brigham Young University, Provo, Utah), David R. Terry, and Richard D. Sagers. Amino acid composition of walls from single and filamentous cells of Clostridium acidiurici. J. Bacteriol. 91:1618-1624. 1966.-The walls from single and filamentous cells of Clostridium acidiurici were shown to contain 11 ami...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.4.1618-1624.1966
更新日期:1966-04-01 00:00:00
abstract::MinD binds to phospholipid vesicles in the presence of ATP and is released by MinE, which stimulates the MinD ATPase. Membrane binding requires a short conserved C-terminal region, which has the potential to form an amphipathic helix. This finding has led to a model in which the binding of ATP regulates the formation ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.15.4326-4335.2003
更新日期:2003-08-01 00:00:00
abstract::Earlier observations that phenol is a repellent for Salmonella typhimurium but an attractant for Escherichia coli were confirmed. This behavioral difference was found to correlate with a difference in the effect phenol had on receptor methylation levels; it caused net demethylation in S. typhimurium but net methylatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.1.371-379.1987
更新日期:1987-01-01 00:00:00
abstract::The Escherichia coli pap genetic determinant includes 11 genes and encodes expression of Pap pili on the bacterial surface. An RNase E-dependent mRNA-processing event in the intercistronic papB-papA region results in the accumulation of a papA-gene-specific mRNA in considerable excess of the primary papB-papA mRNA tra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.3.683-690.1996
更新日期:1996-02-01 00:00:00
abstract::Sinorhizobium meliloti is a soil-dwelling alphaproteobacterium that engages in a nitrogen-fixing root nodule symbiosis with leguminous plants. Cell surface polysaccharides are important both for adapting to stresses in the soil and for the development of an effective symbiotic interaction. Among the polysaccharides ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00501-17
更新日期:2018-01-10 00:00:00
abstract::Some Escherichia coli strains with impaired cell division form branched cells at high frequencies during certain growth conditions. Here, we show that neither FtsI nor FtsZ activity is required for the development of branches. Buds did not form at specific positions along the cell surface during high-branching conditi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.21.6607-6614.1999
更新日期:1999-11-01 00:00:00
abstract::Inducible cat and erm genes are regulated by translational attenuation. In this regulatory model, gene activation results from chloramphenicol- or erythromycin-dependent stalling of a ribosome at a precise site in the leader region of cat or erm transcripts. The stalled ribosome is believed to destabilize a downstream...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.11.6282-6290.1990
更新日期:1990-11-01 00:00:00
abstract::Addition of urea to an uninduced culture of Saccharomyces at 22 C results in appearance of allophanate hydrolase activity after a lag of 12 min. We have previously demonstrated that both ribonucleic acid (RNA) and protein synthesis are needed for this induction to occur. To elucidate the time intervals occupied by kno...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.198-204.1976
更新日期:1976-04-01 00:00:00
abstract::Many pathogenic bacteria translocate virulence factors into their eukaryotic hosts by means of type IV secretion systems (T4SS) spanning the inner and outer membranes. Genes encoding components of these systems have been identified within the order Rickettsiales based upon their sequence similarities to other prototyp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00301-20
更新日期:2020-11-04 00:00:00
abstract::Phase-partitioning studies of the euryhaline bacterium Halomonas elongata demonstrated that the hydrophobic-hydrophilic nature of the cell surface changed as the bacterium grew in different NaCl concentrations. Mid-log-phase cells grown in a high (3.4 M) NaCl concentration were more hydrophilic than were cells grown i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.132-135.1988
更新日期:1988-01-01 00:00:00
abstract::Clostridium perfringens food poisoning is caused by type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe), while C. perfringens-associated non-food-borne gastrointestinal (GI) diseases are caused by isolates carrying a plasmid-borne cpe gene (P-cpe). C. perfringens spores are thought to be the importan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01748-07
更新日期:2008-02-01 00:00:00
abstract::A 16-kb BamHI fragment of the lactose plasmid pNZ63 from Leuconostoc lactis NZ6009 was cloned in Escherichia coli MC1061 by using pACYC184 and was found to express a functional beta-galactosidase. Deletion and complementation analysis showed that the coding region for beta-galactosidase was located on a 5.8-kb SalI-Ba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.13.4475-4481.1992
更新日期:1992-07-01 00:00:00
abstract::Spore formation of Myxococcus xanthus can occur not only on agar plates during fruiting body formation, but also in a liquid culture by simply adding glycerol, dimethyl sulfoxide, or phenethyl alcohol to the culture. This chemically-induced spore formation occurs synchronously and much faster than that occurring durin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.3.1076-1082.1980
更新日期:1980-12-01 00:00:00
abstract::Sequences of the 16S ribosomal DNA (rDNA) from psychrotolerant and mesophilic strains of the Bacillus cereus group revealed signatures which were specific for these two thermal groups of bacteria. Further analysis of the genomic DNA from a wide range of food and soil isolates showed that B. cereus group strains have b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.8.2624-2630.1999
更新日期:1999-04-01 00:00:00
abstract::A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.3.854-859.1984
更新日期:1984-12-01 00:00:00
abstract::The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique patter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.10.3551-3571.2006
更新日期:2006-05-01 00:00:00