The tryptophanase gene cluster of Haemophilus influenzae type b: evidence for horizontal gene transfer.

abstract：:In bacteria, normal mutation frequencies are mostly around 10(-10) per base pair. However, there exists natural isolates, called "mutators," that exhibit permanent mutation occurrences up to 1,000-fold greater than usual. As mutations play essential roles, particularly in the evolution of antibiotic resistance, bacter...

journal_title：Journal of bacteriology

authors： Le Bars H,Bousarghin L,Bonnaure-Mallet M,Jolivet-Gougeon A,Barloy-Hubler F

更新日期：2012-07-01 00:00:00

abstract：:The TatA and TatB proteins are essential components of the twin arginine protein translocation pathway in Escherichia coli. C-terminal truncation analysis of the TatA protein revealed that a plasmid-expressed TatA protein shortened by 40 amino acids is still fully competent to support protein translocation. Similar tr...

journal_title：Journal of bacteriology

authors： Lee PA,Buchanan G,Stanley NR,Berks BC,Palmer T

更新日期：2002-11-01 00:00:00

abstract：:Pneumocystis carinii remains an important opportunistic fungal pathogen causing life-threatening pneumonia in patients with AIDS and malignancy. Currently, little is known about how the organism adapts to environmental stresses and maintains its cellular integrity. We recently discovered an open reading frame approxim...

journal_title：Journal of bacteriology

authors： Kottom TJ,Thomas CF Jr,Limper AH

更新日期：2001-12-01 00:00:00

abstract：:Mutant strains of Escherichia coli K-12 have been isolated in which the synthesis of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase (trp) is partially constitutive. The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus design...

journal_title：Journal of bacteriology

authors： Camakaris J,Pittard J

更新日期：1971-08-01 00:00:00

abstract：:Arginine utilization in Pseudomonas aeruginosa with multiple catabolic pathways represents one of the best examples of the metabolic versatility of this organism. To identify genes involved in arginine catabolism, we have employed DNA microarrays to analyze the transcriptional profiles of this organism in response to ...

journal_title：Journal of bacteriology

authors： Yang Z,Lu CD

更新日期：2007-06-01 00:00:00

abstract：:Streptococcus agalactiae (group B streptococcus [GBS]) is a major cause of infections in newborns, pregnant women, and immunocompromised patients. GBS strain CNCTC10/84 is a clinical isolate that has high virulence in animal models of infection and has been used extensively to study GBS pathogenesis. Two unusual featu...

journal_title：Journal of bacteriology

authors： Zhu L,Beres SB,Yerramilli P,Pruitt L,Cantu CC,Olsen RJ,Musser JM

更新日期：2020-11-04 00:00:00

abstract：:Assays of CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (CGPTase) (EC 2.7.8.12) in membranes isolated from Bacillus subtilis 168 wild type and 11 strains bearing conditional lethal thermosensitive mutations in tagB, tagD, or tagF revealed that CGPTase deficiency was associated only with mutant tagF all...

journal_title：Journal of bacteriology

authors： Pooley HM,Abellan FX,Karamata D

更新日期：1992-01-01 00:00:00

abstract：:The transduction efficiency of Tn551, a staphylococcal transposon coding for erythromycin resistance (ermB), was increased by a factor of about five in acceptor strains carrying the gene for methicillin resistance (mec), as compared with methicillin-sensitive strains. This enhancement was independent of the generalize...

journal_title：Journal of bacteriology

authors： Berger-Bächi B

更新日期：1983-04-01 00:00:00

abstract：:As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...

journal_title：Journal of bacteriology

authors： Lee PJ,Stock AM

更新日期：1996-10-01 00:00:00

abstract：:Bacteroides thetaiotaomicron, a gram-negative obligate anaerobe, appears to utilize starch by first binding the polymer to its surface and then translocating it into the periplasmic space. Several genes that encode enzymes or outer membrane proteins involved in starch utilization have been identified. These have been ...

journal_title：Journal of bacteriology

authors： D'Elia JN,Salyers AA

更新日期：1996-12-01 00:00:00

abstract：:In Escherichia coli the SOS response, induced by DNA-damaging treatments, includes two systems of cell division inhibition, SfiA and SfiC, which are thought to prevent cell division by interacting with the division protein FtsZ. It is shown here that SfiA-mediated division inhibition is readily reversible, even in the...

journal_title：Journal of bacteriology

authors： Maguin E,Lutkenhaus J,D'Ari R

更新日期：1986-06-01 00:00:00

abstract：:The ultimate membrane localization and function of most of the 185 predicted Pseudomonas aeruginosa PAO1 lipoproteins remain unknown. We constructed a fluorescent lipoprotein, CSFP(OmlA)-ChFP, by fusing the signal peptide and the first four amino acids of the P. aeruginosa outer membrane lipoprotein OmlA to the monome...

journal_title：Journal of bacteriology

authors： Lewenza S,Mhlanga MM,Pugsley AP

更新日期：2008-09-01 00:00:00

abstract：:Deoxycytosine methylase (Dcm) enzyme activity causes mutagenesis in vitro either directly by enzyme-induced deamination of cytosine to uracil in the absence of the methyl donor, S-adenosylmethionine (SAM), or indirectly through spontaneous deamination of [5-methyl]cytosine to thymine. Using a Lac reversion assay, we i...

journal_title：Journal of bacteriology

authors： Macintyre G,Atwood CV,Cupples CG

更新日期：2001-02-01 00:00:00

abstract：:Bacteria have evolved multiple strategies to sense and rapidly adapt to challenging and ever-changing environmental conditions. The ability to alter membrane lipid composition, a key component of the cellular envelope, is crucial for bacterial survival and adaptation in response to environmental stress. However, the p...

journal_title：Journal of bacteriology

authors： Rowlett VW,Mallampalli VKPS,Karlstaedt A,Dowhan W,Taegtmeyer H,Margolin W,Vitrac H

更新日期：2017-06-13 00:00:00

abstract：:The isolation of a new thermophilic bacterium, Thermus aquaticus gen. n. and sp. n., is described. Successful enrichment requires incubation at 70 to 75 C, and the use of nutrient media relatively dilute with respect to the organic components. Strains of T. aquaticus have been isolated from a variety of thermal spring...

journal_title：Journal of bacteriology

authors： Brock TD,Freeze H

更新日期：1969-04-01 00:00:00

abstract：:The respiratory control of adhE, which encodes ethanol (alcohol) dehydrogenase in Escherichia coli, was examined at the transcriptional level by using various phi (adhE'-lacZ) adhE+ merodiploid strains. Expression of the adhE'-lacZ operon fusion was increased about eightfold by anaerobic growth. Under anaerobic growth...

journal_title：Journal of bacteriology

authors： Chen YM,Lin EC

更新日期：1991-12-01 00:00:00

abstract：:Tn5 was used to generate mutants that were deficient in the dissimilatory reduction of nitrite for Pseudomonas sp. strain G-179, which contains a copper nitrite reductase. Three types of mutants were isolated. The first type showed a lack of growth on nitrate, nitrite, and nitrous oxide. The second type grew on nitrat...

journal_title：Journal of bacteriology

authors： Ye RW,Averill BA,Tiedje JM

更新日期：1992-10-01 00:00:00

abstract：:Vibrio vulnificus is an estuarine bacterium and potent opportunistic human pathogen. It enters the food chain by asymptomatically colonizing a variety of marine organisms, most notably oysters. Expression of the brp-encoded extracellular polysaccharide, which enhances cell-surface adherence, is regulated by cyclic di-...

journal_title：Journal of bacteriology

authors： Chodur DM,Guo L,Pu M,Bruger E,Fernandez N,Waters C,Rowe-Magnus DA

更新日期：2017-09-05 00:00:00

abstract：:Escherichia coli cells are shown to be attracted to the l-amino acids alanine, asparagine, aspartate, cysteine, glutamate, glycine, methionine, serine, and threonine, but not to arginine, cystine, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, tryptophan, tyrosine, or valine. Bacteria grown in a pro...

journal_title：Journal of bacteriology

authors： Mesibov R,Adler J

更新日期：1972-10-01 00:00:00

abstract：:PcsB is a protein of unknown function that plays a critical role in cell division in Streptococcus pneumoniae and other ovococcus species of Streptococcus. We constructed isogenic sets of mutants expressing different amounts of PcsB in laboratory strain R6 and virulent serotype 2 strain D39 to evaluate its cellular ro...

journal_title：Journal of bacteriology

authors： Barendt SM,Land AD,Sham LT,Ng WL,Tsui HC,Arnold RJ,Winkler ME

更新日期：2009-05-01 00:00:00

abstract：:Yeast mutants deficient in orotidine-5'-phosphate (OMP) pyrophosphorylase activity have been obtained. The gene encoding this enzyme is unlinked to the four pyrimidine genes previously described. The specific activity of OMP pyrophosphorylase remains constant under all of the physiological conditions used to repress, ...

journal_title：Journal of bacteriology

authors： Jund R,Lacroute F

更新日期：1972-01-01 00:00:00

abstract：:rpoS, a gene that encodes an alternative sigma factor (also known as katF), is critical for the ability of Yersinia enterocolitica grown at 37 degrees C, but not at 26 degrees C, to survive diverse environmental insults such as high temperature, hydrogen peroxide, osmolarity, and low pH. However, a Y. enterocolitica r...

journal_title：Journal of bacteriology

authors： Badger JL,Miller VL

更新日期：1995-09-01 00:00:00

abstract：:A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...

journal_title：Journal of bacteriology

authors： Day JM,Janssen GR

更新日期：2004-10-01 00:00:00

abstract：:A circular genetic map of Bacillus stearothermophilus NUB36 was constructed by transduction with bacteriophage TP-42C and protoplast fusion. Sixty-four genes were tentatively assigned a cognate Bacillus subtilis gene based on growth response to intermediates or end products of metabolism, cross-feeding, accumulation o...

journal_title：Journal of bacteriology

authors： Vallier H,Welker NE

更新日期：1990-02-01 00:00:00

abstract：:A group of enzymes were prepared from the culture fluids of streptococci belonging to groups A, B, C, G, and L, and from a strain of Streptococcus sanguis. These streptococcal enzymes (designated St-sialidases) released a substance shown to belong to the sialic acid group from the specific substrate BSM-St, a sialomuc...

journal_title：Journal of bacteriology

authors： Hayano S,Tanaka A

更新日期：1969-03-01 00:00:00

abstract：:The well-known large catabolic potential of rhodococci is greatly facilitated by an impressive gene multiplicity. This study reports on the multiplicity of kshA, encoding the oxygenase component of 3-ketosteroid 9α-hydroxylase, a key enzyme in steroid catabolism. Five kshA homologues (kshA1 to kshA5) were previously i...

journal_title：Journal of bacteriology

authors： Petrusma M,Hessels G,Dijkhuizen L,van der Geize R

更新日期：2011-08-01 00:00:00

abstract：:When Agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (DNA) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. Such association is not...

journal_title：Journal of bacteriology

authors： Yajko DM,Hegeman GD

更新日期：1971-12-01 00:00:00

abstract：:We purified membrane-bound cytochrome c-550 [cytochrome c-550(m)] to an electrophoretically homogeneous state from Nitrobacter winogradskyi. The cytochrome showed peaks at 409 and 525 nm in the oxidized form and peaks at 416, 521, and 550 nm in the reduced form. The molecular weight of the cytochrome was estimated to ...

journal_title：Journal of bacteriology

authors： Nomoto T,Fukumori Y,Yamanaka T

更新日期：1993-07-01 00:00:00

abstract：:The messenger ribonucleic acid (mRNA) pattern of log-phase and sporulating cells of Bacillus subtilis has been analyzed by deoxyribonucleic acid (DNA)-RNA hybrid studies with the complementary-strand fragments of DNA. Approximately 80% of the mRNA of log-phase and sporulating cells from stages I, III, and IV hybridize...

journal_title：Journal of bacteriology

authors： Sumida-Yasumoto C,Doi RH

更新日期：1974-02-01 00:00:00

abstract：:When heat-activated spores of Bacillus cereus T (thy(-)) were germinated and grown in medium containing (3)H-thymidine, a significant amount of radioactivity was incorporated into ribonucleic acid and deoxyribonucleic acid (DNA). A method was developed to restrict the incorporation of radioactivity from (3)H-thymidine...

journal_title：Journal of bacteriology

authors： Rana RS,Halvorson HO

更新日期：1972-02-01 00:00:00