Truncation analysis of TatA and TatB defines the minimal functional units required for protein translocation.

abstract：:S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...

journal_title：Journal of bacteriology

authors： Thibaut D,Couder M,Crouzet J,Debussche L,Cameron B,Blanche F

更新日期：1990-11-01 00:00:00

abstract：:A characteristic of N2-fixing cyanobacteria in symbiotic associations appears to be release of N2-derived NH4+. The specific activity of the primary ammonium-assimilating enzyme, glutamine synthetase (GS), was found to be three- to fourfold lower in Nostoc sp. strain 7801 grown in symbiotic association with the bryoph...

journal_title：Journal of bacteriology

authors： Joseph CM,Meeks JC

更新日期：1987-06-01 00:00:00

abstract：:The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP...

journal_title：Journal of bacteriology

authors： Wu CY,Blaszczak LC,Smith MC,Skatrud PL

更新日期：1994-03-01 00:00:00

abstract：:The temperate phage TPW22, induced from Lactococcus lactis subsp. cremoris W22, and the evolutionarily interesting integrase of this phage were characterized. Phage TPW22 was propagated lytically on L. lactis subsp. cremoris 3107, which could also be lysogenized by site-specific integration. The attachment site (attP)...

journal_title：Journal of bacteriology

authors： Petersen A,Josephsen J,Johnsen MG

更新日期：1999-11-01 00:00:00

abstract：:Streptomyces coelicolor Müller contains two superoxide dismutases (SODs), nickel-containing (NiSOD) and iron- and zinc-containing SOD (FeZnSOD). The sodF gene encoding FeZnSOD was isolated by using PCR primers corresponding to the N-terminal peptide sequence of the purified FeZnSOD and a C-terminal region conserved am...

journal_title：Journal of bacteriology

authors： Kim EJ,Chung HJ,Suh B,Hah YC,Roe JH

更新日期：1998-04-01 00:00:00

abstract：:Mechanisms of Li+ stimulation of proline transport were studied in cells of Escherichia coli 7 and NR70, a mutant of strain 7 lacking adenosine triphosphatase (EC 3.6.1.3). An electrochemical potential difference of Li+ induced in an inward direction of energy-depleted cells caused a transient uptake of proline depend...

journal_title：Journal of bacteriology

authors： Kayama-Gonda Y,Kawasaki T

更新日期：1979-08-01 00:00:00

abstract：:Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...

journal_title：Journal of bacteriology

authors： LeVier K,Walker GC

更新日期：2001-11-01 00:00:00

abstract：:Whole-genome sequence analysis of Mycobacterium leprae has revealed a limited number of protein-coding genes, with half of the genome composed of pseudogenes and noncoding regions. We previously showed that some M. leprae pseudogenes are transcribed at high levels and that their expression levels change following infe...

journal_title：Journal of bacteriology

authors： Akama T,Suzuki K,Tanigawa K,Kawashima A,Wu H,Nakata N,Osana Y,Sakakibara Y,Ishii N

更新日期：2009-05-01 00:00:00

abstract：:The staphylococcal virulon is activated by the density-sensing agr system, which is autoinduced by a short peptide (autoinducing peptide [AIP]) processed from a propeptide encoded by agrD. A central segment of the agr locus, consisting of the C-terminal two-thirds of AgrB (the putative processing enzyme), AgrD, and th...

journal_title：Journal of bacteriology

authors： Jarraud S,Lyon GJ,Figueiredo AM,Lina G,Vandenesch F,Etienne J,Muir TW,Novick RP

更新日期：2000-11-01 00:00:00

abstract：:Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cyste...

journal_title：Journal of bacteriology

authors： Mansilla MC,de Mendoza D

更新日期：1997-02-01 00:00:00

abstract：:The metabolism of one- and two-carbon compounds by the methylotrophic bacterium Methylobacterium extorquens AM1 involves high carbon flux through the ethylmalonyl coenzyme A (ethylmalonyl-CoA) pathway (EMC pathway). During growth on ethylamine, the EMC pathway operates as a linear pathway carrying the full assimilator...

journal_title：Journal of bacteriology

authors： Good NM,Martinez-Gomez NC,Beck DA,Lidstrom ME

更新日期：2015-02-15 00:00:00

abstract：:MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...

journal_title：Journal of bacteriology

authors： van den Brink-van der Laan E,Boots JW,Spelbrink RE,Kool GM,Breukink E,Killian JA,de Kruijff B

更新日期：2003-07-01 00:00:00

abstract：:The conjugation system of the IncP alpha plasmid RK2/RP4 is encoded by transfer regions designated Tra1, Tra2, and Tra3. The Tra1 core region, cloned on plasmid pDG4 delta 22, consists of the origin of transfer (oriT) and 2.6 kilobases of flanking DNA providing IncP alpha plasmid-specific functions that allow pDG4 del...

journal_title：Journal of bacteriology

authors： Guiney DG,Deiss C,Simnad V,Yee L,Pansegrau W,Lanka E

更新日期：1989-07-01 00:00:00

abstract：:Autoinducer-2 (AI-2) is required for biofilm formation and virulence of the oral pathogen Aggregatibacter actinomycetemcomitans, and we previously showed that lsrB codes for a receptor for AI-2. The lsrB gene is expressed as part of the lsrACDBFG operon, which is divergently transcribed from an adjacent lsrRK operon. ...

journal_title：Journal of bacteriology

authors： Torres-Escobar A,Juárez-Rodríguez MD,Lamont RJ,Demuth DR

更新日期：2013-01-01 00:00:00

abstract：UNLABELLED:It is well established that the RpoN-RpoS sigma factor (σ(54)-σ(S)) cascade plays an essential role in differential gene expression during the enzootic cycle of Borrelia burgdorferi, the causative agent of Lyme disease. The RpoN-RpoS pathway is activated by the response regulator/σ(54)-dependent activator (a...

journal_title：Journal of bacteriology

authors： Yin Y,Yang Y,Xiang X,Wang Q,Yang ZN,Blevins J,Lou Y,Yang XF

更新日期：2016-04-28 00:00:00

abstract：:Phosphatidylglycerol, the most abundant acidic phospholipid in Escherichia coli, is considered to play specific roles in various cellular processes that are essential for cell viability. A null mutation of pgsA, which encodes phosphatidylglycerophosphate synthase, does indeed confer lethality. However, pgsA null mutan...

journal_title：Journal of bacteriology

authors： Suzuki M,Hara H,Matsumoto K

更新日期：2002-10-01 00:00:00

abstract：:Total synthesis of acetate from CO2 by Clostridium acidiurici during fermentations of hypoxanthine has been shown to involve synthesis of glycine from methylenetetrahydrofolate, CO2, and NH3. The glycine is converted to serine by the addition of methylenetetrahydrofolate, and the resulting serine is converted to pyruv...

journal_title：Journal of bacteriology

authors： Waber LJ,Wood HG

更新日期：1979-11-01 00:00:00

abstract：:The ability of deoxyribonucleic acid (DNA) isolated from a number of actinomycetes to reassociate with reference DNA from Actinoplanes philippinensis or Streptomyces venezuelae has been measured. All of the DNA preparations except for those from Nocardia erythropolis and Thermomonospora viridis contained 70 to 73 mole...

journal_title：Journal of bacteriology

authors： Farina G,Bradley SG

更新日期：1970-04-01 00:00:00

abstract：:LcrV, an essential piece of the Yop virulon, is encoded by the large lcrGVsycDyopBD operon. In spite of repeated efforts, the role of LcrV in the Yop virulon remains elusive. In an attempt to clarify this, we engineered a complete deletion of lcrV in the pYV plasmid of Yersinia enterocolitica E40 and characterized the...

journal_title：Journal of bacteriology

authors： Sarker MR,Neyt C,Stainier I,Cornelis GR

更新日期：1998-03-01 00:00:00

abstract：:We have constructed a suicide vector, pU1800, containing the transposable element TnphoA (Tn5 IS50L::phoA), for the purpose of producing protein fusions in vivo between the Escherichia coli alkaline phosphatase (APase) and proteins of the facultative photoheterotroph, Rhodobacter sphaeroides. We introduced TnphoA into...

journal_title：Journal of bacteriology

authors： Moore MD,Kaplan S

更新日期：1989-08-01 00:00:00

abstract：:The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...

journal_title：Journal of bacteriology

authors： Nelson DR,Rittenberg SC

更新日期：1981-09-01 00:00:00

abstract：:The complete nucleotide sequence of the linear plasmid pBD2 from Rhodococcus erythropolis BD2 comprises 210,205 bp. Sequence analyses of pBD2 revealed 212 putative open reading frames (ORFs), 97 of which had an annotatable function. These ORFs could be assigned to six functional groups: plasmid replication and mainten...

journal_title：Journal of bacteriology

authors： Stecker C,Johann A,Herzberg C,Averhoff B,Gottschalk G

更新日期：2003-09-01 00:00:00

abstract：:Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEA...

journal_title：Journal of bacteriology

authors： Bonato MC,da Costa Maia JC,Juliani MH

更新日期：1983-06-01 00:00:00

abstract：:A series of R (rough) Salmonella minnesota mutants with rfb, rfe, and rfa mutations leading to various defects in the biosynthesis of cell wall lipopolysaccharide was analyzed as to their enterobacterial common antigen (CA) content. All mutants that had functional rfe genes were CA(+) as is the wild-type parent. This ...

journal_title：Journal of bacteriology

authors： Mäkelä PH,Mayer H,Whang HY,Neter E

更新日期：1974-09-01 00:00:00

abstract：:Acid titrations of intact and butanol-treated cells of Streptococcus faecalis revealed that nearly all of the intracellular K(+) ions could diffuse into the suspending medium in association with small anions, including ribonucleic acid breakdown products, when the cell membrane was damaged. In contrast, nearly all of ...

journal_title：Journal of bacteriology

authors： Marquis RE,Porterfield N,Matsumura P

更新日期：1973-05-01 00:00:00

abstract：:Lüderitz, O. (Max-Planck-Institut für Immunbiologie, Frieburg, Germany), H. J. Risse, H. Schulte-Holthausen, J. L. Strominger, I. W. Sutherland, and O. Westphal. Biochemical studies of the smooth-rough mutation in Salmonella minnesota. J. Bacteriol. 89:343-354. 1965.-A comparative study of the O antigen from the smoot...

journal_title：Journal of bacteriology

authors： LUEDERITZ O,RISSE HJ,SCHULTE-HOLTHAUSEN H,STROMINGER JL,SUTHERLAND IW,WESTPHAL O

更新日期：1965-02-01 00:00:00

abstract：:A study of the distribution of pyrrolidone carboxylyl peptidase (PCP) activity among cell fractions of Streptococcus cremoris HP revealed that this enzyme is associated with a particulate fraction, which mainly consists of membrane material. This location could only be established using a gentle nonmechanical method f...

journal_title：Journal of bacteriology

authors： Exterkate FA

更新日期：1977-03-01 00:00:00

abstract：:Streptomyces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B(2)) analog. Here, we report the 9,466,619-bp linear chromosome of S. davawensis JCM 4913 and a 89,331-bp linear plasmid. The sequence has an average G+C content of 70.58% and contains six rRNA operons (16S-23S-5...

journal_title：Journal of bacteriology

authors： Jankowitsch F,Schwarz J,Rückert C,Gust B,Szczepanowski R,Blom J,Pelzer S,Kalinowski J,Mack M

更新日期：2012-12-01 00:00:00

abstract：:Members of the pPT23A plasmid family of Pseudomonas syringae play an important role in the interaction of this bacterial pathogen with host plants. Complete sequence analysis of several pPT23A family plasmids (PFPs) has provided a glimpse of the gene content and virulence function of these plasmids. We constructed a m...

journal_title：Journal of bacteriology

authors： Zhao Y,Ma Z,Sundin GW

更新日期：2005-03-01 00:00:00

abstract：:Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...

journal_title：Journal of bacteriology

authors： Price KE,Camilli A

更新日期：2009-04-01 00:00:00