Abstract:
:We have constructed a suicide vector, pU1800, containing the transposable element TnphoA (Tn5 IS50L::phoA), for the purpose of producing protein fusions in vivo between the Escherichia coli alkaline phosphatase (APase) and proteins of the facultative photoheterotroph, Rhodobacter sphaeroides. We introduced TnphoA into the genome of R. sphaeroides at a coupled conjugation-transposition frequency of approximately 1 x 10(-6). Fusions giving rise to APase expression, as judged by blue-colony pigmentation when exconjugants were plated on growth medium containing the chromogenic indicator 5-bromo-4-chloro-3-indolyl phosphate, were observed in about 1% of the exconjugants. Numerous, distinguishable mutant phenotypes have been generated by this method, including those which lack the ability to use dimethyl sulfoxide as a terminal electron acceptor during anaerobic respiration, as well as those which are photosynthetically incompetent or altered in pigment synthesis, and others that express resistance to chlorate. The growth and spectral characteristics of several of these mutants, as well as the localization and quantitation of subcellular APase activity under different physiological conditions, have been examined. The presence of TnphoA in the host genome has been confirmed for each mutant analyzed, and specifically tagged DNA fragments containing TnphoA have been identified and localized; cosmids containing R. sphaeroides genomic DNA capable of complementing individual mutants have also been isolated. The usefulness of this approach in studying gene activity in R. sphaeroides is discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Moore MD,Kaplan Sdoi
10.1128/jb.171.8.4385-4394.1989subject
Has Abstractpub_date
1989-08-01 00:00:00pages
4385-94issue
8eissn
0021-9193issn
1098-5530journal_volume
171pub_type
杂志文章abstract::Using a sensitive primer extension technique, we have carried out studies to localize the start site of replication of the replicon RepFIC. In the course of these studies, we have found evidence that supports the hypothesis that transcription is an integral component of the initiation of replication. On the basis of o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.7.2163-2168.1997
更新日期:1997-04-01 00:00:00
abstract::In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3429-3438.1992
更新日期:1992-06-01 00:00:00
abstract::Green fluorescent protein (GFP) fusions, immunofluorescence microscopy, and cryo-electron tomography revealed that the chemoreceptors of the Lyme disease spirochete Borrelia burgdorferi form long, thin arrays near both cell poles. These arrays are in close proximity to the flagellar motors. This information provides a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01530-10
更新日期:2011-05-01 00:00:00
abstract::We identified in Listeria monocytogenes a gene encoding a protein homologous to MecA, a regulatory protein acting with ClpC and ComK in the competence pathway of Bacillus subtilis. In L. monocytogenes, MecA is involved, along with ClpC and ClpP, in the downregulation of a 64-kDa secreted protein. In B. subtilis, the M...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.20.5931-5934.2000
更新日期:2000-10-01 00:00:00
abstract::The rates of synthesis of Aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a Mg(++)-free medium. Deoxyribonucleic acid (DNA) synthesized during Mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.345-356.1967
更新日期:1967-01-01 00:00:00
abstract::Partial denaturation of Saccharomyces cerevisiae chromosomal DNA was found to occur spontaneously during meiosis. Short regions of strand separation (300 base pairs long) were seen in DNA molecules prepared for electron microscopy by the aqueous spreading technique. These regions were clustered along the DNA. The time...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.629-635.1978
更新日期:1978-05-01 00:00:00
abstract::Alysiella bovis adheres to surfaces by means of short, ruthenium red-staining, rod-like fimbriae. The fimbriae remain associated with the cell envelope of A. bovis, even when sonicated or exposed sequentially to toluene, Triton X-100, lysozyme, ribonuclease, and deoxyribonuclease. Adhesion of outer membrane-derived ce...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.569-576.1984
更新日期:1984-11-01 00:00:00
abstract::A citrate lyase (EC 4.1.3.6) was purified 25-fold from Leuconostoc mesenteroides and was shown to contain three subunits. The first 42 amino acids of the beta subunit were identified, as well as an internal peptide sequence spanning some 20 amino acids into the alpha subunit. Using degenerated primers from these seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.3.647-654.1998
更新日期:1998-02-01 00:00:00
abstract::Cells of Pseudomonas stutzeri are naturally transformed by homologous chromosomal DNA; they do not require chemical treatment to become competent. This capacity to undergo natural transformation was found to be shared by the closely related species P. mendocina, P. alcaligenes, and P. pseudoalcaligenes, but was not de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.93-99.1983
更新日期:1983-01-01 00:00:00
abstract::Biofilms are structured multicellular communities of bacteria that form through a developmental process. In standing culture, undomesticated strains of Bacillus subtilis produce a floating biofilm, called a pellicle, with a distinct macroscopic architecture. Here we report on a comprehensive analysis of B. subtilis pe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00157-07
更新日期:2007-07-01 00:00:00
abstract::Type 3 fimbriae are adhesive organelles found in enterobacterial pathogens. The fimbriae promote biofilm formation on biotic and abiotic surfaces; however, the exact identity of the receptor for the type 3 fimbriae adhesin, MrkD, remains elusive. We analyzed naturally occurring structural and functional variabilities ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00753-13
更新日期:2013-12-01 00:00:00
abstract::Escherichia coli K-12 can utilize a gamma-glutamyl peptide as an amino acid source, for which gamma-glutamyltranspeptidase (EC 2.3.2.2) is essential. We suggest that the gamma-glutamyl linkage of a gamma-glutamyl peptide is hydrolyzed by gamma-glutamyltranspeptidase located in the periplasmic space, and the released a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.18.6038-6040.1993
更新日期:1993-09-01 00:00:00
abstract::The opportunistic bacterial pathogen Vibrio vulnificus causes severe wound infection and fatal septicemia. We used alkaline phosphatase insertion mutagenesis in a clinical isolate of V. vulnificus to find genes necessary for virulence, and we identified fadR, which encodes a regulator of fatty acid metabolism. The fad...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01016-08
更新日期:2008-12-01 00:00:00
abstract::Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C. jejuni-mediated enteric disease is unclear; however, epitopes associated with the LOS are linked to the development of neurologic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.8.2100-2107.2002
更新日期:2002-04-01 00:00:00
abstract::The isolation of a Streptococcus thermophilus CNRZ368 mutant displaying a long-chain phenotype allowed us to identify the cse gene (for cellular segregation). The N terminus of Cse exhibits high similarity to Streptococcus agalactiae surface immunogenic protein (SIP), while its C terminus exhibits high similarity to S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.8.2737-2746.2005
更新日期:2005-04-01 00:00:00
abstract::Exfoliative toxin type B is produced by bacteriophage group II strains of Staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. In addition to exfoliative toxin B, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. These phenotypes, as well as re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.574-580.1986
更新日期:1986-05-01 00:00:00
abstract::Analysis of polypeptides in the crystalline delta-endotoxins from different Bacillus thuringiensis strains revealed two antigenically similar forms of the P2 protein which differed in molecular mass, peptide profile, and amino acid sequence. Purified preparations of the two forms displayed the characteristic dual toxi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.5141-5147.1989
更新日期:1989-09-01 00:00:00
abstract::The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4828-4837.1988
更新日期:1988-10-01 00:00:00
abstract::A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.7-14.1967
更新日期:1967-01-01 00:00:00
abstract::Comprehensive analysis of genome-wide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysaccharide substrates was undertaken with the goal of proposing carbohydrate specificities for transport systems and putative transcriptional regulators. Saccharid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.21.7267-7282.2005
更新日期:2005-11-01 00:00:00
abstract::Chimeric plasmids, which were useful as cloning vehicles in a Streptococcus sanguis (Challis) host vector system, have been constructed. By using three different strategies of restriction endonuclease digestion and ligation, a deoxyribonucleic acid (DNA) fragment bearing an erythromycin resistance determinant was liga...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.3.1425-1435.1980
更新日期:1980-09-01 00:00:00
abstract::Type 1 secretion systems (T1SS) are present in a wide range of Gram-negative bacteria and are involved in the secretion of diverse substrates such as proteases, lipases, and hemophores. T1SS consist of three proteins: an inner membrane ABC (ATP binding cassette) protein, a periplasmic adaptor, and an outer membrane ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00178-10
更新日期:2010-08-01 00:00:00
abstract::In vitro deoxyribonucleic acid (DNA) synthesis systems based on an earlier system using pencillin have been developed which use osmotic lysis of lysozyme-formed spheroplasts of Escherichia coli cells embedded in an agarose matrix. An adenosine 5'-triphosphate (ATP)-dependent semiconservative mode, or replicative mode,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.1.159-170.1975
更新日期:1975-04-01 00:00:00
abstract::High-pressure liquid chromatography of a muramidase digest of murein sacculi from Caulobacter crescentus showed that the absence of D-alanine carboxypeptidase activity in the cells was reflected by a very high content of pentapeptide in the murein. Approximately half of the pentapeptide side chains were shown to conta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.649-655.1983
更新日期:1983-11-01 00:00:00
abstract::Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modificat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00763-06
更新日期:2006-12-01 00:00:00
abstract::Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing. The present study reveals physiological conditions and promoter DNA elements that regulate Escherichia coli pfs transcription. Pfs transcripti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00868-06
更新日期:2006-11-01 00:00:00
abstract::The Brevibacterium acetylicum gsk gene, which encodes guanosine kinase (ATP:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the N-terminal amino acid sequence of the purified protein. The cloned chromosomal fragment containing the gsk gene was sequenc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.22.6959-6964.1997
更新日期:1997-11-01 00:00:00
abstract::A previously undetected Bacillus subtilis aspartokinase isozyme, which we have called aspartokinase III, has been characterized. The new isozyme was most readily detected in extracts of cells grown with lysine, which repressed aspartokinase II and induced aspartokinase III, or in extracts of strain VS11, a mutant lack...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.218-223.1990
更新日期:1990-01-01 00:00:00
abstract::Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.8.2555-2562.2003
更新日期:2003-04-01 00:00:00
abstract::In Klebsiella pneumoniae, products of the nitrogen fixation nifLA operon regulate transcription of the other nif operons. NifA activates transcription by sigma54-holoenzyme. In vivo, NifL antagonizes the action of NifA under aerobic conditions or in the presence of combined nitrogen. In contrast to a previous report, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4679-4687.1996
更新日期:1996-08-01 00:00:00